Transcriptomic profiles of human livers undergoing rewarming machine perfusion before transplantation—first insights
Machine perfusion by controlled oxygenated rewarming (COR) is feasible and safe in clinical application and result in a promising outcome. This study utilizes next-generation sequencing (NGS) to investigate the transcriptome of human liver tissue undergoing COR before liver transplantation. Cold-sto...
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| Veröffentlicht in: | Functional & integrative genomics 2021-07, Vol.21 (3-4), p.367-376 |
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| creator | Hoyer, Dieter Paul Swoboda, Sandra Treckmann, Juergen Walter Benkö, Tamas Paul, Andreas Brocke-Ahmadinejad, Nahal |
| description | Machine perfusion by controlled oxygenated rewarming (COR) is feasible and safe in clinical application and result in a promising outcome. This study utilizes next-generation sequencing (NGS) to investigate the transcriptome of human liver tissue undergoing COR before liver transplantation. Cold-stored livers were subjected to machine-assisted slow COR for ~120 min before transplantation. Biopsies were taken before (preCOR) and after COR (postCOR) and 1 h after reperfusion (postRep). The samples were sequenced, using RNA-seq to analyze differential transcriptional changes between the different stages and treatments of the grafts. Comparison of differential gene expression preCOR and postCOR demonstrated 10 upregulated genes. postRep 97 and 178 genes were upregulated and 7 and 13 downregulated compared to preCOR and postCOR, respectively. A shift of gene expressions by machine perfusion to the TGF-beta pathway was observed. The present study demonstrates distinct transcriptome profiles associated with machine perfusion by COR and transplantation of human livers. Such data provide a deeper understanding of the molecular mechanisms of machine perfusion technology in human liver transplantation. |
| doi_str_mv | 10.1007/s10142-021-00781-0 |
| format | Article |
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This study utilizes next-generation sequencing (NGS) to investigate the transcriptome of human liver tissue undergoing COR before liver transplantation. Cold-stored livers were subjected to machine-assisted slow COR for ~120 min before transplantation. Biopsies were taken before (preCOR) and after COR (postCOR) and 1 h after reperfusion (postRep). The samples were sequenced, using RNA-seq to analyze differential transcriptional changes between the different stages and treatments of the grafts. Comparison of differential gene expression preCOR and postCOR demonstrated 10 upregulated genes. postRep 97 and 178 genes were upregulated and 7 and 13 downregulated compared to preCOR and postCOR, respectively. A shift of gene expressions by machine perfusion to the TGF-beta pathway was observed. The present study demonstrates distinct transcriptome profiles associated with machine perfusion by COR and transplantation of human livers. Such data provide a deeper understanding of the molecular mechanisms of machine perfusion technology in human liver transplantation.</description><identifier>ISSN: 1438-793X</identifier><identifier>EISSN: 1438-7948</identifier><identifier>DOI: 10.1007/s10142-021-00781-0</identifier><identifier>PMID: 33733319</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Aged ; Animal Genetics and Genomics ; Biochemistry ; Bioinformatics ; Biomedical and Life Sciences ; Cell Biology ; Cryopreservation ; Female ; Gene expression ; Humans ; Life Sciences ; Liver - metabolism ; Liver transplantation ; Liver Transplantation - methods ; Male ; Microbial Genetics and Genomics ; Middle Aged ; Molecular modelling ; Next-generation sequencing ; Organ Preservation ; Original ; Original Article ; Perfusion ; Perfusion - instrumentation ; Perfusion - methods ; Plant Genetics and Genomics ; Reperfusion ; Rewarming ; Transcription ; Transcriptome ; Transcriptomes</subject><ispartof>Functional & integrative genomics, 2021-07, Vol.21 (3-4), p.367-376</ispartof><rights>The Author(s) 2021</rights><rights>2021. 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This study utilizes next-generation sequencing (NGS) to investigate the transcriptome of human liver tissue undergoing COR before liver transplantation. Cold-stored livers were subjected to machine-assisted slow COR for ~120 min before transplantation. Biopsies were taken before (preCOR) and after COR (postCOR) and 1 h after reperfusion (postRep). The samples were sequenced, using RNA-seq to analyze differential transcriptional changes between the different stages and treatments of the grafts. Comparison of differential gene expression preCOR and postCOR demonstrated 10 upregulated genes. postRep 97 and 178 genes were upregulated and 7 and 13 downregulated compared to preCOR and postCOR, respectively. A shift of gene expressions by machine perfusion to the TGF-beta pathway was observed. The present study demonstrates distinct transcriptome profiles associated with machine perfusion by COR and transplantation of human livers. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Functional & integrative genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoyer, Dieter Paul</au><au>Swoboda, Sandra</au><au>Treckmann, Juergen Walter</au><au>Benkö, Tamas</au><au>Paul, Andreas</au><au>Brocke-Ahmadinejad, Nahal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptomic profiles of human livers undergoing rewarming machine perfusion before transplantation—first insights</atitle><jtitle>Functional & integrative genomics</jtitle><stitle>Funct Integr Genomics</stitle><addtitle>Funct Integr Genomics</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>21</volume><issue>3-4</issue><spage>367</spage><epage>376</epage><pages>367-376</pages><issn>1438-793X</issn><eissn>1438-7948</eissn><abstract>Machine perfusion by controlled oxygenated rewarming (COR) is feasible and safe in clinical application and result in a promising outcome. This study utilizes next-generation sequencing (NGS) to investigate the transcriptome of human liver tissue undergoing COR before liver transplantation. Cold-stored livers were subjected to machine-assisted slow COR for ~120 min before transplantation. Biopsies were taken before (preCOR) and after COR (postCOR) and 1 h after reperfusion (postRep). The samples were sequenced, using RNA-seq to analyze differential transcriptional changes between the different stages and treatments of the grafts. Comparison of differential gene expression preCOR and postCOR demonstrated 10 upregulated genes. postRep 97 and 178 genes were upregulated and 7 and 13 downregulated compared to preCOR and postCOR, respectively. A shift of gene expressions by machine perfusion to the TGF-beta pathway was observed. The present study demonstrates distinct transcriptome profiles associated with machine perfusion by COR and transplantation of human livers. 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| subjects | Aged Animal Genetics and Genomics Biochemistry Bioinformatics Biomedical and Life Sciences Cell Biology Cryopreservation Female Gene expression Humans Life Sciences Liver - metabolism Liver transplantation Liver Transplantation - methods Male Microbial Genetics and Genomics Middle Aged Molecular modelling Next-generation sequencing Organ Preservation Original Original Article Perfusion Perfusion - instrumentation Perfusion - methods Plant Genetics and Genomics Reperfusion Rewarming Transcription Transcriptome Transcriptomes |
| title | Transcriptomic profiles of human livers undergoing rewarming machine perfusion before transplantation—first insights |
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