Methods Evaluation for Rapid Concentration and Quantification of SARS-CoV-2 in Raw Wastewater Using Droplet Digital and Quantitative RT-PCR

Wastewater surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging public health tool to understand the spread of Coronavirus Disease 2019 (COVID-19) in communities. The performance of different virus concentration methods and PCR methods needs to be evaluated to...

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Veröffentlicht in:	Food and environmental virology 2021-09, Vol.13 (3), p.303-315
Hauptverfasser:	Flood, Matthew T., D’Souza, Nishita, Rose, Joan B., Aw, Tiong Gim
Format:	Artikel
Sprache:	eng
Schlagworte:	Biomedical and Life Sciences Biomedicine Chemical Fractionation - methods Chemical Precipitation Chemistry/Food Science Coronaviruses COVID-19 Droplets Environmental Monitoring Food Science Genetic markers Manholes Markers Original Paper Phages Polyethylene glycol Polyethylene Glycols - chemistry Polymerase chain reaction Public Health Raw wastewater Recovery Reverse Transcriptase Polymerase Chain Reaction - methods Reverse transcription SARS-CoV-2 - genetics SARS-CoV-2 - isolation & purification Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Sewage Sewage - chemistry Sewage - virology Ultrafiltration Ultrafiltration - methods Viral diseases Viral envelope proteins Viral Proteins - genetics Virology Viruses Waste Water - chemistry Waste Water - virology Wastewater treatment Wastewater treatment plants Water Pollution - analysis
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