Spatially resolved qualified sewage spot sampling to track SARS-CoV-2 dynamics in Munich - One year of experience
Wastewater-based epidemiology (WBE) is a tool now increasingly proposed to monitor the SARS-CoV-2 burden in populations without the need for individual mass testing. It is especially interesting in metropolitan areas where spread can be very fast, and proper sewage systems are available for sampling...
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| Veröffentlicht in: | The Science of the total environment 2021-11, Vol.797, p.149031-149031, Article 149031 |
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| creator | Rubio-Acero, Raquel Beyerl, Jessica Muenchhoff, Maximilian Castelletti, Noemi Paunovic, Ivana Springer, Bernd Nagel, Christian Boehm, Bernhard Graf, Alexander Krebs, Stefan Keppler, Oliver T. Osterman, Andreas Khan, Zohaib Nisar Hoelscher, Michael Wieser, Andreas Jared, Anderson Abhishek, Bakuli Olimbek, Bemirayev Rebecca, Böhnlein Isabel, Brand Jan, Bruger Noemi, Castelletti Josephine, Coleman Lorenzo, Contento Alina, Czwienzek Flora, Deák Diefenbach Maximilian, N. Jürgen, Durner Ute, Eberle Tabea, Eser Philine, Falk Manuela, Feyereisen Felix, Forster Turid, Frahnow Jonathan, Frese Christiane, Fuchs Otto, Geisenberger Kristina, Gillig Elias, Golschan Michelle, Guggenbuehl Noller Jessica Pablo, Gutierrez Anslem, Haderer Lena, Hartinger Jan, Hasenauer Alejandra, Hernandez Tim, Hofberger Michael, Hölscher Kristina, Huber Christian, Janke Antonia, Keßler Zohaib, Khan Johanna, Kresin Inge, Kroidl Arne, Kroidl Magdalena, Lang Clemens, Lang Silvan, Lange Michael, Laxy Ronan, Le Gleut Reiner, Leidl Leopold, Liedl Xhovana, Lucaj Sophie, Nafziger Alexandra Alisa, Markgraf Rebecca, Mayrhofer Dafni, Metaxa Laura, Olbrich Ivana, Paunovic Michael, Plank Claire, Pleimelding Stephan, Prückner Kerstin, Puchinger Katja, Radon Jakob, Reich Friedrich, Riess Camilla, Rothe Raquel, Rubio-Acero Viktoria, Ruci Elmar, Saathoff Nicole, Schäfer Yannik, Schälte Lara, Schneider Mirjam, Schunk Alba, Soler Kathrin, Strobl Jeni, Tang Fabian, Theis Verena, Thiel Sophie, Thiesbrummel Emilia, Von Lovenberg Jonathan, Von Lovenberg Claudia, Wallrauch Andreas, Wieser Simon, Winter Roman, Wölfel Julia, Wolff Tobias, Würfel Sabine, Zange Eleftheria, Zeggini Thorbjörn, Zimmer |
| description | Wastewater-based epidemiology (WBE) is a tool now increasingly proposed to monitor the SARS-CoV-2 burden in populations without the need for individual mass testing. It is especially interesting in metropolitan areas where spread can be very fast, and proper sewage systems are available for sampling with short flow times and thus little decay of the virus. We started in March 2020 to set up a once-a-week qualified spot sampling protocol in six different locations in Munich carefully chosen to contain primarily wastewater of permanent residential areas, rather than industry or hospitals. We used RT-PCR and sequencing to track the spread of SARS-CoV-2 in the Munich population with temporo-spatial resolution.
The study became fully operational in mid-April 2020 and has been tracking SARS-CoV-2 RNA load weekly for one year. Sequencing of the isolated viral RNA was performed to obtain information about the presence and abundance of variants of concern in the Munich area over time.
We demonstrate that the evolution of SARS-CoV-2 RNA loads (between |
| doi_str_mv | 10.1016/j.scitotenv.2021.149031 |
| format | Article |
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Beyerl, Jessica ; Muenchhoff, Maximilian ; Castelletti, Noemi ; Paunovic, Ivana ; Springer, Bernd ; Nagel, Christian ; Boehm, Bernhard ; Graf, Alexander ; Krebs, Stefan ; Keppler, Oliver T. ; Osterman, Andreas ; Khan, Zohaib Nisar ; Hoelscher, Michael ; Wieser, Andreas ; Jared, Anderson ; Abhishek, Bakuli ; Olimbek, Bemirayev ; Rebecca, Böhnlein ; Isabel, Brand ; Jan, Bruger ; Noemi, Castelletti ; Josephine, Coleman ; Lorenzo, Contento ; Alina, Czwienzek ; Flora, Deák ; Diefenbach Maximilian, N. ; Jürgen, Durner ; Ute, Eberle ; Tabea, Eser ; Philine, Falk ; Manuela, Feyereisen ; Felix, Forster ; Turid, Frahnow ; Jonathan, Frese ; Christiane, Fuchs ; Otto, Geisenberger ; Kristina, Gillig ; Elias, Golschan ; Michelle, Guggenbuehl Noller Jessica ; Pablo, Gutierrez ; Anslem, Haderer ; Lena, Hartinger ; Jan, Hasenauer ; Alejandra, Hernandez ; Tim, Hofberger ; Michael, Hölscher ; Kristina, Huber ; Christian, Janke ; Antonia, Keßler ; Zohaib, Khan ; Johanna, Kresin ; Inge, Kroidl ; Arne, Kroidl ; Magdalena, Lang ; Clemens, Lang ; Silvan, Lange ; Michael, Laxy ; Ronan, Le Gleut ; Reiner, Leidl ; Leopold, Liedl ; Xhovana, Lucaj ; Sophie, Nafziger Alexandra ; Alisa, Markgraf ; Rebecca, Mayrhofer ; Dafni, Metaxa ; Laura, Olbrich ; Ivana, Paunovic ; Michael, Plank ; Claire, Pleimelding ; Stephan, Prückner ; Kerstin, Puchinger ; Katja, Radon ; Jakob, Reich ; Friedrich, Riess ; Camilla, Rothe ; Raquel, Rubio-Acero ; Viktoria, Ruci ; Elmar, Saathoff ; Nicole, Schäfer ; Yannik, Schälte ; Lara, Schneider ; Mirjam, Schunk ; Alba, Soler ; Kathrin, Strobl ; Jeni, Tang ; Fabian, Theis ; Verena, Thiel ; Sophie, Thiesbrummel ; Emilia, Von Lovenberg ; Jonathan, Von Lovenberg ; Claudia, Wallrauch ; Andreas, Wieser ; Simon, Winter ; Roman, Wölfel ; Julia, Wolff ; Tobias, Würfel ; Sabine, Zange ; Eleftheria, Zeggini ; Thorbjörn, Zimmer</creator><creatorcontrib>Rubio-Acero, Raquel ; Beyerl, Jessica ; Muenchhoff, Maximilian ; Castelletti, Noemi ; Paunovic, Ivana ; Springer, Bernd ; Nagel, Christian ; Boehm, Bernhard ; Graf, Alexander ; Krebs, Stefan ; Keppler, Oliver T. ; Osterman, Andreas ; Khan, Zohaib Nisar ; Hoelscher, Michael ; Wieser, Andreas ; Jared, Anderson ; Abhishek, Bakuli ; Olimbek, Bemirayev ; Rebecca, Böhnlein ; Isabel, Brand ; Jan, Bruger ; Noemi, Castelletti ; Josephine, Coleman ; Lorenzo, Contento ; Alina, Czwienzek ; Flora, Deák ; Diefenbach Maximilian, N. ; Jürgen, Durner ; Ute, Eberle ; Tabea, Eser ; Philine, Falk ; Manuela, Feyereisen ; Felix, Forster ; Turid, Frahnow ; Jonathan, Frese ; Christiane, Fuchs ; Otto, Geisenberger ; Kristina, Gillig ; Elias, Golschan ; Michelle, Guggenbuehl Noller Jessica ; Pablo, Gutierrez ; Anslem, Haderer ; Lena, Hartinger ; Jan, Hasenauer ; Alejandra, Hernandez ; Tim, Hofberger ; Michael, Hölscher ; Kristina, Huber ; Christian, Janke ; Antonia, Keßler ; Zohaib, Khan ; Johanna, Kresin ; Inge, Kroidl ; Arne, Kroidl ; Magdalena, Lang ; Clemens, Lang ; Silvan, Lange ; Michael, Laxy ; Ronan, Le Gleut ; Reiner, Leidl ; Leopold, Liedl ; Xhovana, Lucaj ; Sophie, Nafziger Alexandra ; Alisa, Markgraf ; Rebecca, Mayrhofer ; Dafni, Metaxa ; Laura, Olbrich ; Ivana, Paunovic ; Michael, Plank ; Claire, Pleimelding ; Stephan, Prückner ; Kerstin, Puchinger ; Katja, Radon ; Jakob, Reich ; Friedrich, Riess ; Camilla, Rothe ; Raquel, Rubio-Acero ; Viktoria, Ruci ; Elmar, Saathoff ; Nicole, Schäfer ; Yannik, Schälte ; Lara, Schneider ; Mirjam, Schunk ; Alba, Soler ; Kathrin, Strobl ; Jeni, Tang ; Fabian, Theis ; Verena, Thiel ; Sophie, Thiesbrummel ; Emilia, Von Lovenberg ; Jonathan, Von Lovenberg ; Claudia, Wallrauch ; Andreas, Wieser ; Simon, Winter ; Roman, Wölfel ; Julia, Wolff ; Tobias, Würfel ; Sabine, Zange ; Eleftheria, Zeggini ; Thorbjörn, Zimmer ; on behalf of the KoCo19-Study Group ; KoCo19-Study Group</creatorcontrib><description>Wastewater-based epidemiology (WBE) is a tool now increasingly proposed to monitor the SARS-CoV-2 burden in populations without the need for individual mass testing. It is especially interesting in metropolitan areas where spread can be very fast, and proper sewage systems are available for sampling with short flow times and thus little decay of the virus. We started in March 2020 to set up a once-a-week qualified spot sampling protocol in six different locations in Munich carefully chosen to contain primarily wastewater of permanent residential areas, rather than industry or hospitals. We used RT-PCR and sequencing to track the spread of SARS-CoV-2 in the Munich population with temporo-spatial resolution.
The study became fully operational in mid-April 2020 and has been tracking SARS-CoV-2 RNA load weekly for one year. Sequencing of the isolated viral RNA was performed to obtain information about the presence and abundance of variants of concern in the Munich area over time.
We demonstrate that the evolution of SARS-CoV-2 RNA loads (between <7.5 and 3874/ml) in these different areas within Munich correlates well with official seven day incidence notification data (between 0.0 and 327 per 100,000) obtained from the authorities within the respective region. Wastewater viral loads predicted the dynamic of SARS-CoV-2 local incidence about 3 weeks in advance of data based on respiratory swab analyses. Aligning with multiple different point-mutations characteristic for certain variants of concern, we could demonstrate the gradual increase of variant of concern B.1.1.7 in the Munich population beginning in January 2021, weeks before it became apparent in sequencing results of swabs samples taken from patients living in Munich.
Overall, the study highlights the potential of WBE to monitor the SARS-CoV-2 pandemic, including the introduction of variants of concern in a local population.
[Display omitted]
•Waste-water based epidemiology can be convenient for representative SARS-CoV-2 sampling in metropolitan areas.•38ml qualified spot sampled sewage once weekly enabled the tracking of SARS-CoV-2 dynamics in Munich over a one-year period.•Ultracentrifugation combined with standard RNA extraction and RT-PCR/ddPCR ensure easy SARS-CoV-2 detection at a low cost.•RNA extract sequencing detects presence and frequencies of variants of concern prior to frequent detection in swab samples.</description><identifier>ISSN: 0048-9697</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2021.149031</identifier><identifier>PMID: 34346361</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>B.1.1.7 ; COVID-19 ; Humans ; PCR ; RNA, Viral ; SARS-CoV-2 ; Sequencing ; Sewage ; Surveillance ; Wastewater</subject><ispartof>The Science of the total environment, 2021-11, Vol.797, p.149031-149031, Article 149031</ispartof><rights>2021</rights><rights>Copyright © 2021. Published by Elsevier B.V.</rights><rights>2021 Published by Elsevier B.V. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-6409f3350e31e2152d056f1db306abdd283e411c0dcb51004e6972567975221c3</citedby><cites>FETCH-LOGICAL-c541t-6409f3350e31e2152d056f1db306abdd283e411c0dcb51004e6972567975221c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0048969721041036$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34346361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rubio-Acero, Raquel</creatorcontrib><creatorcontrib>Beyerl, Jessica</creatorcontrib><creatorcontrib>Muenchhoff, Maximilian</creatorcontrib><creatorcontrib>Castelletti, Noemi</creatorcontrib><creatorcontrib>Paunovic, Ivana</creatorcontrib><creatorcontrib>Springer, Bernd</creatorcontrib><creatorcontrib>Nagel, Christian</creatorcontrib><creatorcontrib>Boehm, Bernhard</creatorcontrib><creatorcontrib>Graf, Alexander</creatorcontrib><creatorcontrib>Krebs, Stefan</creatorcontrib><creatorcontrib>Keppler, Oliver T.</creatorcontrib><creatorcontrib>Osterman, Andreas</creatorcontrib><creatorcontrib>Khan, Zohaib Nisar</creatorcontrib><creatorcontrib>Hoelscher, Michael</creatorcontrib><creatorcontrib>Wieser, Andreas</creatorcontrib><creatorcontrib>Jared, Anderson</creatorcontrib><creatorcontrib>Abhishek, Bakuli</creatorcontrib><creatorcontrib>Olimbek, Bemirayev</creatorcontrib><creatorcontrib>Rebecca, Böhnlein</creatorcontrib><creatorcontrib>Isabel, Brand</creatorcontrib><creatorcontrib>Jan, Bruger</creatorcontrib><creatorcontrib>Noemi, Castelletti</creatorcontrib><creatorcontrib>Josephine, Coleman</creatorcontrib><creatorcontrib>Lorenzo, Contento</creatorcontrib><creatorcontrib>Alina, Czwienzek</creatorcontrib><creatorcontrib>Flora, Deák</creatorcontrib><creatorcontrib>Diefenbach Maximilian, N.</creatorcontrib><creatorcontrib>Jürgen, Durner</creatorcontrib><creatorcontrib>Ute, Eberle</creatorcontrib><creatorcontrib>Tabea, Eser</creatorcontrib><creatorcontrib>Philine, Falk</creatorcontrib><creatorcontrib>Manuela, Feyereisen</creatorcontrib><creatorcontrib>Felix, Forster</creatorcontrib><creatorcontrib>Turid, Frahnow</creatorcontrib><creatorcontrib>Jonathan, Frese</creatorcontrib><creatorcontrib>Christiane, Fuchs</creatorcontrib><creatorcontrib>Otto, Geisenberger</creatorcontrib><creatorcontrib>Kristina, Gillig</creatorcontrib><creatorcontrib>Elias, Golschan</creatorcontrib><creatorcontrib>Michelle, Guggenbuehl Noller Jessica</creatorcontrib><creatorcontrib>Pablo, Gutierrez</creatorcontrib><creatorcontrib>Anslem, Haderer</creatorcontrib><creatorcontrib>Lena, Hartinger</creatorcontrib><creatorcontrib>Jan, Hasenauer</creatorcontrib><creatorcontrib>Alejandra, Hernandez</creatorcontrib><creatorcontrib>Tim, Hofberger</creatorcontrib><creatorcontrib>Michael, Hölscher</creatorcontrib><creatorcontrib>Kristina, Huber</creatorcontrib><creatorcontrib>Christian, Janke</creatorcontrib><creatorcontrib>Antonia, Keßler</creatorcontrib><creatorcontrib>Zohaib, Khan</creatorcontrib><creatorcontrib>Johanna, Kresin</creatorcontrib><creatorcontrib>Inge, Kroidl</creatorcontrib><creatorcontrib>Arne, Kroidl</creatorcontrib><creatorcontrib>Magdalena, Lang</creatorcontrib><creatorcontrib>Clemens, Lang</creatorcontrib><creatorcontrib>Silvan, Lange</creatorcontrib><creatorcontrib>Michael, Laxy</creatorcontrib><creatorcontrib>Ronan, Le Gleut</creatorcontrib><creatorcontrib>Reiner, Leidl</creatorcontrib><creatorcontrib>Leopold, Liedl</creatorcontrib><creatorcontrib>Xhovana, Lucaj</creatorcontrib><creatorcontrib>Sophie, Nafziger Alexandra</creatorcontrib><creatorcontrib>Alisa, Markgraf</creatorcontrib><creatorcontrib>Rebecca, Mayrhofer</creatorcontrib><creatorcontrib>Dafni, Metaxa</creatorcontrib><creatorcontrib>Laura, Olbrich</creatorcontrib><creatorcontrib>Ivana, Paunovic</creatorcontrib><creatorcontrib>Michael, Plank</creatorcontrib><creatorcontrib>Claire, Pleimelding</creatorcontrib><creatorcontrib>Stephan, Prückner</creatorcontrib><creatorcontrib>Kerstin, Puchinger</creatorcontrib><creatorcontrib>Katja, Radon</creatorcontrib><creatorcontrib>Jakob, Reich</creatorcontrib><creatorcontrib>Friedrich, Riess</creatorcontrib><creatorcontrib>Camilla, Rothe</creatorcontrib><creatorcontrib>Raquel, Rubio-Acero</creatorcontrib><creatorcontrib>Viktoria, Ruci</creatorcontrib><creatorcontrib>Elmar, Saathoff</creatorcontrib><creatorcontrib>Nicole, Schäfer</creatorcontrib><creatorcontrib>Yannik, Schälte</creatorcontrib><creatorcontrib>Lara, Schneider</creatorcontrib><creatorcontrib>Mirjam, Schunk</creatorcontrib><creatorcontrib>Alba, Soler</creatorcontrib><creatorcontrib>Kathrin, Strobl</creatorcontrib><creatorcontrib>Jeni, Tang</creatorcontrib><creatorcontrib>Fabian, Theis</creatorcontrib><creatorcontrib>Verena, Thiel</creatorcontrib><creatorcontrib>Sophie, Thiesbrummel</creatorcontrib><creatorcontrib>Emilia, Von Lovenberg</creatorcontrib><creatorcontrib>Jonathan, Von Lovenberg</creatorcontrib><creatorcontrib>Claudia, Wallrauch</creatorcontrib><creatorcontrib>Andreas, Wieser</creatorcontrib><creatorcontrib>Simon, Winter</creatorcontrib><creatorcontrib>Roman, Wölfel</creatorcontrib><creatorcontrib>Julia, Wolff</creatorcontrib><creatorcontrib>Tobias, Würfel</creatorcontrib><creatorcontrib>Sabine, Zange</creatorcontrib><creatorcontrib>Eleftheria, Zeggini</creatorcontrib><creatorcontrib>Thorbjörn, Zimmer</creatorcontrib><creatorcontrib>on behalf of the KoCo19-Study Group</creatorcontrib><creatorcontrib>KoCo19-Study Group</creatorcontrib><title>Spatially resolved qualified sewage spot sampling to track SARS-CoV-2 dynamics in Munich - One year of experience</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>Wastewater-based epidemiology (WBE) is a tool now increasingly proposed to monitor the SARS-CoV-2 burden in populations without the need for individual mass testing. It is especially interesting in metropolitan areas where spread can be very fast, and proper sewage systems are available for sampling with short flow times and thus little decay of the virus. We started in March 2020 to set up a once-a-week qualified spot sampling protocol in six different locations in Munich carefully chosen to contain primarily wastewater of permanent residential areas, rather than industry or hospitals. We used RT-PCR and sequencing to track the spread of SARS-CoV-2 in the Munich population with temporo-spatial resolution.
The study became fully operational in mid-April 2020 and has been tracking SARS-CoV-2 RNA load weekly for one year. Sequencing of the isolated viral RNA was performed to obtain information about the presence and abundance of variants of concern in the Munich area over time.
We demonstrate that the evolution of SARS-CoV-2 RNA loads (between <7.5 and 3874/ml) in these different areas within Munich correlates well with official seven day incidence notification data (between 0.0 and 327 per 100,000) obtained from the authorities within the respective region. Wastewater viral loads predicted the dynamic of SARS-CoV-2 local incidence about 3 weeks in advance of data based on respiratory swab analyses. Aligning with multiple different point-mutations characteristic for certain variants of concern, we could demonstrate the gradual increase of variant of concern B.1.1.7 in the Munich population beginning in January 2021, weeks before it became apparent in sequencing results of swabs samples taken from patients living in Munich.
Overall, the study highlights the potential of WBE to monitor the SARS-CoV-2 pandemic, including the introduction of variants of concern in a local population.
[Display omitted]
•Waste-water based epidemiology can be convenient for representative SARS-CoV-2 sampling in metropolitan areas.•38ml qualified spot sampled sewage once weekly enabled the tracking of SARS-CoV-2 dynamics in Munich over a one-year period.•Ultracentrifugation combined with standard RNA extraction and RT-PCR/ddPCR ensure easy SARS-CoV-2 detection at a low cost.•RNA extract sequencing detects presence and frequencies of variants of concern prior to frequent detection in swab samples.</description><subject>B.1.1.7</subject><subject>COVID-19</subject><subject>Humans</subject><subject>PCR</subject><subject>RNA, 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Thiel</creator><creator>Sophie, Thiesbrummel</creator><creator>Emilia, Von Lovenberg</creator><creator>Jonathan, Von Lovenberg</creator><creator>Claudia, Wallrauch</creator><creator>Andreas, Wieser</creator><creator>Simon, Winter</creator><creator>Roman, Wölfel</creator><creator>Julia, Wolff</creator><creator>Tobias, Würfel</creator><creator>Sabine, Zange</creator><creator>Eleftheria, Zeggini</creator><creator>Thorbjörn, Zimmer</creator><general>Elsevier B.V</general><general>Published by Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20211125</creationdate><title>Spatially resolved qualified sewage spot sampling to track SARS-CoV-2 dynamics in Munich - One year of experience</title><author>Rubio-Acero, Raquel ; Beyerl, Jessica ; Muenchhoff, Maximilian ; Castelletti, Noemi ; Paunovic, Ivana ; Springer, Bernd ; Nagel, Christian ; Boehm, Bernhard ; Graf, Alexander ; Krebs, Stefan ; Keppler, Oliver T. ; Osterman, Andreas ; Khan, Zohaib Nisar ; Hoelscher, Michael ; Wieser, Andreas ; Jared, Anderson ; Abhishek, Bakuli ; Olimbek, Bemirayev ; Rebecca, Böhnlein ; Isabel, Brand ; Jan, Bruger ; Noemi, Castelletti ; Josephine, Coleman ; Lorenzo, Contento ; Alina, Czwienzek ; Flora, Deák ; Diefenbach Maximilian, N. ; Jürgen, Durner ; Ute, Eberle ; Tabea, Eser ; Philine, Falk ; Manuela, Feyereisen ; Felix, Forster ; Turid, Frahnow ; Jonathan, Frese ; Christiane, Fuchs ; Otto, Geisenberger ; Kristina, Gillig ; Elias, Golschan ; Michelle, Guggenbuehl Noller Jessica ; Pablo, Gutierrez ; Anslem, Haderer ; Lena, Hartinger ; Jan, Hasenauer ; Alejandra, Hernandez ; Tim, Hofberger ; Michael, Hölscher ; Kristina, Huber ; Christian, Janke ; Antonia, Keßler ; Zohaib, Khan ; Johanna, Kresin ; Inge, Kroidl ; Arne, Kroidl ; Magdalena, Lang ; Clemens, Lang ; Silvan, Lange ; Michael, Laxy ; Ronan, Le Gleut ; Reiner, Leidl ; Leopold, Liedl ; Xhovana, Lucaj ; Sophie, Nafziger Alexandra ; Alisa, Markgraf ; Rebecca, Mayrhofer ; Dafni, Metaxa ; Laura, Olbrich ; Ivana, Paunovic ; Michael, Plank ; Claire, Pleimelding ; Stephan, Prückner ; Kerstin, Puchinger ; Katja, Radon ; Jakob, Reich ; Friedrich, Riess ; Camilla, Rothe ; Raquel, Rubio-Acero ; Viktoria, Ruci ; Elmar, Saathoff ; Nicole, Schäfer ; Yannik, Schälte ; Lara, Schneider ; Mirjam, Schunk ; Alba, Soler ; Kathrin, Strobl ; Jeni, Tang ; Fabian, Theis ; Verena, Thiel ; Sophie, Thiesbrummel ; Emilia, Von Lovenberg ; Jonathan, Von Lovenberg ; Claudia, Wallrauch ; Andreas, Wieser ; Simon, Winter ; Roman, Wölfel ; Julia, Wolff ; Tobias, Würfel ; Sabine, Zange ; Eleftheria, Zeggini ; Thorbjörn, Zimmer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-6409f3350e31e2152d056f1db306abdd283e411c0dcb51004e6972567975221c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>B.1.1.7</topic><topic>COVID-19</topic><topic>Humans</topic><topic>PCR</topic><topic>RNA, Viral</topic><topic>SARS-CoV-2</topic><topic>Sequencing</topic><topic>Sewage</topic><topic>Surveillance</topic><topic>Wastewater</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rubio-Acero, Raquel</creatorcontrib><creatorcontrib>Beyerl, Jessica</creatorcontrib><creatorcontrib>Muenchhoff, Maximilian</creatorcontrib><creatorcontrib>Castelletti, Noemi</creatorcontrib><creatorcontrib>Paunovic, Ivana</creatorcontrib><creatorcontrib>Springer, Bernd</creatorcontrib><creatorcontrib>Nagel, Christian</creatorcontrib><creatorcontrib>Boehm, Bernhard</creatorcontrib><creatorcontrib>Graf, Alexander</creatorcontrib><creatorcontrib>Krebs, Stefan</creatorcontrib><creatorcontrib>Keppler, Oliver T.</creatorcontrib><creatorcontrib>Osterman, Andreas</creatorcontrib><creatorcontrib>Khan, Zohaib Nisar</creatorcontrib><creatorcontrib>Hoelscher, Michael</creatorcontrib><creatorcontrib>Wieser, Andreas</creatorcontrib><creatorcontrib>Jared, Anderson</creatorcontrib><creatorcontrib>Abhishek, Bakuli</creatorcontrib><creatorcontrib>Olimbek, Bemirayev</creatorcontrib><creatorcontrib>Rebecca, Böhnlein</creatorcontrib><creatorcontrib>Isabel, Brand</creatorcontrib><creatorcontrib>Jan, Bruger</creatorcontrib><creatorcontrib>Noemi, Castelletti</creatorcontrib><creatorcontrib>Josephine, Coleman</creatorcontrib><creatorcontrib>Lorenzo, Contento</creatorcontrib><creatorcontrib>Alina, Czwienzek</creatorcontrib><creatorcontrib>Flora, Deák</creatorcontrib><creatorcontrib>Diefenbach Maximilian, N.</creatorcontrib><creatorcontrib>Jürgen, Durner</creatorcontrib><creatorcontrib>Ute, Eberle</creatorcontrib><creatorcontrib>Tabea, Eser</creatorcontrib><creatorcontrib>Philine, Falk</creatorcontrib><creatorcontrib>Manuela, Feyereisen</creatorcontrib><creatorcontrib>Felix, Forster</creatorcontrib><creatorcontrib>Turid, Frahnow</creatorcontrib><creatorcontrib>Jonathan, Frese</creatorcontrib><creatorcontrib>Christiane, Fuchs</creatorcontrib><creatorcontrib>Otto, Geisenberger</creatorcontrib><creatorcontrib>Kristina, Gillig</creatorcontrib><creatorcontrib>Elias, Golschan</creatorcontrib><creatorcontrib>Michelle, Guggenbuehl Noller Jessica</creatorcontrib><creatorcontrib>Pablo, Gutierrez</creatorcontrib><creatorcontrib>Anslem, Haderer</creatorcontrib><creatorcontrib>Lena, Hartinger</creatorcontrib><creatorcontrib>Jan, Hasenauer</creatorcontrib><creatorcontrib>Alejandra, Hernandez</creatorcontrib><creatorcontrib>Tim, Hofberger</creatorcontrib><creatorcontrib>Michael, Hölscher</creatorcontrib><creatorcontrib>Kristina, Huber</creatorcontrib><creatorcontrib>Christian, Janke</creatorcontrib><creatorcontrib>Antonia, Keßler</creatorcontrib><creatorcontrib>Zohaib, Khan</creatorcontrib><creatorcontrib>Johanna, Kresin</creatorcontrib><creatorcontrib>Inge, Kroidl</creatorcontrib><creatorcontrib>Arne, Kroidl</creatorcontrib><creatorcontrib>Magdalena, Lang</creatorcontrib><creatorcontrib>Clemens, Lang</creatorcontrib><creatorcontrib>Silvan, Lange</creatorcontrib><creatorcontrib>Michael, Laxy</creatorcontrib><creatorcontrib>Ronan, Le Gleut</creatorcontrib><creatorcontrib>Reiner, Leidl</creatorcontrib><creatorcontrib>Leopold, Liedl</creatorcontrib><creatorcontrib>Xhovana, Lucaj</creatorcontrib><creatorcontrib>Sophie, Nafziger Alexandra</creatorcontrib><creatorcontrib>Alisa, Markgraf</creatorcontrib><creatorcontrib>Rebecca, Mayrhofer</creatorcontrib><creatorcontrib>Dafni, Metaxa</creatorcontrib><creatorcontrib>Laura, Olbrich</creatorcontrib><creatorcontrib>Ivana, Paunovic</creatorcontrib><creatorcontrib>Michael, Plank</creatorcontrib><creatorcontrib>Claire, Pleimelding</creatorcontrib><creatorcontrib>Stephan, Prückner</creatorcontrib><creatorcontrib>Kerstin, Puchinger</creatorcontrib><creatorcontrib>Katja, Radon</creatorcontrib><creatorcontrib>Jakob, Reich</creatorcontrib><creatorcontrib>Friedrich, Riess</creatorcontrib><creatorcontrib>Camilla, Rothe</creatorcontrib><creatorcontrib>Raquel, Rubio-Acero</creatorcontrib><creatorcontrib>Viktoria, Ruci</creatorcontrib><creatorcontrib>Elmar, Saathoff</creatorcontrib><creatorcontrib>Nicole, Schäfer</creatorcontrib><creatorcontrib>Yannik, Schälte</creatorcontrib><creatorcontrib>Lara, Schneider</creatorcontrib><creatorcontrib>Mirjam, Schunk</creatorcontrib><creatorcontrib>Alba, Soler</creatorcontrib><creatorcontrib>Kathrin, Strobl</creatorcontrib><creatorcontrib>Jeni, Tang</creatorcontrib><creatorcontrib>Fabian, Theis</creatorcontrib><creatorcontrib>Verena, Thiel</creatorcontrib><creatorcontrib>Sophie, Thiesbrummel</creatorcontrib><creatorcontrib>Emilia, Von Lovenberg</creatorcontrib><creatorcontrib>Jonathan, Von Lovenberg</creatorcontrib><creatorcontrib>Claudia, Wallrauch</creatorcontrib><creatorcontrib>Andreas, Wieser</creatorcontrib><creatorcontrib>Simon, Winter</creatorcontrib><creatorcontrib>Roman, Wölfel</creatorcontrib><creatorcontrib>Julia, Wolff</creatorcontrib><creatorcontrib>Tobias, Würfel</creatorcontrib><creatorcontrib>Sabine, Zange</creatorcontrib><creatorcontrib>Eleftheria, Zeggini</creatorcontrib><creatorcontrib>Thorbjörn, Zimmer</creatorcontrib><creatorcontrib>on behalf of the KoCo19-Study Group</creatorcontrib><creatorcontrib>KoCo19-Study Group</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rubio-Acero, Raquel</au><au>Beyerl, Jessica</au><au>Muenchhoff, Maximilian</au><au>Castelletti, Noemi</au><au>Paunovic, Ivana</au><au>Springer, Bernd</au><au>Nagel, Christian</au><au>Boehm, Bernhard</au><au>Graf, Alexander</au><au>Krebs, Stefan</au><au>Keppler, Oliver T.</au><au>Osterman, Andreas</au><au>Khan, Zohaib Nisar</au><au>Hoelscher, Michael</au><au>Wieser, Andreas</au><au>Jared, Anderson</au><au>Abhishek, Bakuli</au><au>Olimbek, Bemirayev</au><au>Rebecca, Böhnlein</au><au>Isabel, Brand</au><au>Jan, Bruger</au><au>Noemi, Castelletti</au><au>Josephine, Coleman</au><au>Lorenzo, Contento</au><au>Alina, Czwienzek</au><au>Flora, Deák</au><au>Diefenbach Maximilian, N.</au><au>Jürgen, Durner</au><au>Ute, Eberle</au><au>Tabea, Eser</au><au>Philine, Falk</au><au>Manuela, Feyereisen</au><au>Felix, Forster</au><au>Turid, Frahnow</au><au>Jonathan, Frese</au><au>Christiane, Fuchs</au><au>Otto, Geisenberger</au><au>Kristina, Gillig</au><au>Elias, Golschan</au><au>Michelle, Guggenbuehl Noller Jessica</au><au>Pablo, Gutierrez</au><au>Anslem, Haderer</au><au>Lena, Hartinger</au><au>Jan, Hasenauer</au><au>Alejandra, Hernandez</au><au>Tim, Hofberger</au><au>Michael, Hölscher</au><au>Kristina, Huber</au><au>Christian, Janke</au><au>Antonia, Keßler</au><au>Zohaib, Khan</au><au>Johanna, Kresin</au><au>Inge, Kroidl</au><au>Arne, Kroidl</au><au>Magdalena, Lang</au><au>Clemens, Lang</au><au>Silvan, Lange</au><au>Michael, Laxy</au><au>Ronan, Le Gleut</au><au>Reiner, Leidl</au><au>Leopold, Liedl</au><au>Xhovana, Lucaj</au><au>Sophie, Nafziger Alexandra</au><au>Alisa, Markgraf</au><au>Rebecca, Mayrhofer</au><au>Dafni, Metaxa</au><au>Laura, Olbrich</au><au>Ivana, Paunovic</au><au>Michael, Plank</au><au>Claire, Pleimelding</au><au>Stephan, Prückner</au><au>Kerstin, Puchinger</au><au>Katja, Radon</au><au>Jakob, Reich</au><au>Friedrich, Riess</au><au>Camilla, Rothe</au><au>Raquel, Rubio-Acero</au><au>Viktoria, Ruci</au><au>Elmar, Saathoff</au><au>Nicole, Schäfer</au><au>Yannik, Schälte</au><au>Lara, Schneider</au><au>Mirjam, Schunk</au><au>Alba, Soler</au><au>Kathrin, Strobl</au><au>Jeni, Tang</au><au>Fabian, Theis</au><au>Verena, Thiel</au><au>Sophie, Thiesbrummel</au><au>Emilia, Von Lovenberg</au><au>Jonathan, Von Lovenberg</au><au>Claudia, Wallrauch</au><au>Andreas, Wieser</au><au>Simon, Winter</au><au>Roman, Wölfel</au><au>Julia, Wolff</au><au>Tobias, Würfel</au><au>Sabine, Zange</au><au>Eleftheria, Zeggini</au><au>Thorbjörn, Zimmer</au><aucorp>on behalf of the KoCo19-Study Group</aucorp><aucorp>KoCo19-Study Group</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spatially resolved qualified sewage spot sampling to track SARS-CoV-2 dynamics in Munich - One year of experience</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2021-11-25</date><risdate>2021</risdate><volume>797</volume><spage>149031</spage><epage>149031</epage><pages>149031-149031</pages><artnum>149031</artnum><issn>0048-9697</issn><eissn>1879-1026</eissn><abstract>Wastewater-based epidemiology (WBE) is a tool now increasingly proposed to monitor the SARS-CoV-2 burden in populations without the need for individual mass testing. It is especially interesting in metropolitan areas where spread can be very fast, and proper sewage systems are available for sampling with short flow times and thus little decay of the virus. We started in March 2020 to set up a once-a-week qualified spot sampling protocol in six different locations in Munich carefully chosen to contain primarily wastewater of permanent residential areas, rather than industry or hospitals. We used RT-PCR and sequencing to track the spread of SARS-CoV-2 in the Munich population with temporo-spatial resolution.
The study became fully operational in mid-April 2020 and has been tracking SARS-CoV-2 RNA load weekly for one year. Sequencing of the isolated viral RNA was performed to obtain information about the presence and abundance of variants of concern in the Munich area over time.
We demonstrate that the evolution of SARS-CoV-2 RNA loads (between <7.5 and 3874/ml) in these different areas within Munich correlates well with official seven day incidence notification data (between 0.0 and 327 per 100,000) obtained from the authorities within the respective region. Wastewater viral loads predicted the dynamic of SARS-CoV-2 local incidence about 3 weeks in advance of data based on respiratory swab analyses. Aligning with multiple different point-mutations characteristic for certain variants of concern, we could demonstrate the gradual increase of variant of concern B.1.1.7 in the Munich population beginning in January 2021, weeks before it became apparent in sequencing results of swabs samples taken from patients living in Munich.
Overall, the study highlights the potential of WBE to monitor the SARS-CoV-2 pandemic, including the introduction of variants of concern in a local population.
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•Waste-water based epidemiology can be convenient for representative SARS-CoV-2 sampling in metropolitan areas.•38ml qualified spot sampled sewage once weekly enabled the tracking of SARS-CoV-2 dynamics in Munich over a one-year period.•Ultracentrifugation combined with standard RNA extraction and RT-PCR/ddPCR ensure easy SARS-CoV-2 detection at a low cost.•RNA extract sequencing detects presence and frequencies of variants of concern prior to frequent detection in swab samples.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>34346361</pmid><doi>10.1016/j.scitotenv.2021.149031</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0048-9697 |
| ispartof | The Science of the total environment, 2021-11, Vol.797, p.149031-149031, Article 149031 |
| issn | 0048-9697 1879-1026 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8294104 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | B.1.1.7 COVID-19 Humans PCR RNA, Viral SARS-CoV-2 Sequencing Sewage Surveillance Wastewater |
| title | Spatially resolved qualified sewage spot sampling to track SARS-CoV-2 dynamics in Munich - One year of experience |
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