Comprehensive Molecular Profiling of Desmoplastic Small Round Cell Tumor

Desmoplastic small round cell tumor (DSRCT) is characterized by the t(11;22) (p13:q12) translocation. Few additional putative drivers have been identified, and research has suffered from a lack of model systems. Next-generation sequencing (NGS) data from 68 matched tumor-normal samples, whole-genome...

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Veröffentlicht in:Molecular cancer research 2021-07, Vol.19 (7), p.1146-1155
Hauptverfasser: Slotkin, Emily K, Bowman, Anita S, Levine, Max F, Dela Cruz, Filemon, Coutinho, Diego F, Sanchez, Glorymar I, Rosales, Nestor, Modak, Shakeel, Tap, William D, Gounder, Mrinal M, Thornton, Katherine A, Bouvier, Nancy, You, Daoqi, Gundem, Gunes, Gerstle, Justin T, Heaton, Todd E, LaQuaglia, Michael P, Wexler, Leonard H, Meyers, Paul A, Kung, Andrew L, Papaemmanuil, Elli, Zehir, Ahmet, Ladanyi, Marc, Shukla, Neerav
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Zusammenfassung:Desmoplastic small round cell tumor (DSRCT) is characterized by the t(11;22) (p13:q12) translocation. Few additional putative drivers have been identified, and research has suffered from a lack of model systems. Next-generation sequencing (NGS) data from 68 matched tumor-normal samples, whole-genome sequencing data from 10 samples, transcriptomic and affymetrix array data, and a bank of DSRCT patient-derived xenograft (PDX) are presented. fusions were noted to be simple, balanced events. Recurrent mutations were uncommon, but were noted in (3%), (6%), (5%), and (3%), and recurrent loss of heterozygosity (LOH) at 11p, 11q, and 16q was identified in 18%, 22%, and 34% of samples, respectively. Comparison of tumor-normal matched versus unmatched analysis suggests overcalling of somatic mutations in prior publications of DSRCT NGS data. Alterations in fibroblast growth factor receptor 4 ( ) were identified in 5 of 68 (7%) of tumor samples, whereas differential overexpression of was confirmed orthogonally using 2 platforms. PDX models harbored the pathognomic fusion and were highly representative of corresponding tumors. Our analyses confirm DSRCT as a genomically quiet cancer defined by the balanced translocation, t(11;22)(p13:q12), characterized by a paucity of secondary mutations but a significant number of copy number alterations. Against this genomically quiet background, recurrent activating alterations of stood out, and suggest that this receptor tyrosine kinase, also noted to be highly expressed in DSRCT, should be further investigated. Future studies of DSRCT biology and preclinical therapeutic strategies should benefit from the PDX models characterized in this study. IMPLICATIONS: These data describe the general quiescence of the desmoplastic small round cell tumor (DSRCT) genome, present the first available bank of DSRCT model systems, and nominate as a key receptor tyrosine kinase in DSRCT, based on high expression, recurrent amplification, and recurrent activating mutations.
ISSN:1541-7786
1557-3125
DOI:10.1158/1541-7786.MCR-20-0722