Geochemical compositional controls on DNA strand breaks induced in in vitro cell-free assays by crushed rock powders from the Panasqueira mine area, Portugal
DNA strand breaks are a common form of DNA damage that can contribute to chromosomal instability or gene mutations. Such strand breaks may be caused by exposure to heavy metals. The aim of this study was to assess the level of DNA strand breaks caused by µm-scale solid particles of known chemical co...
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| Veröffentlicht in: | Environmental geochemistry and health 2021-07, Vol.43 (7), p.2631-2647 |
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| creator | Badri, Hatim Polya, David A. Povey, Andrew. C. |
| description | DNA strand breaks are a common form of DNA damage that can contribute to chromosomal instability or gene mutations. Such strand breaks may be caused by exposure to heavy metals. The aim of this study was to assess the level of DNA strand breaks caused by µm-scale solid particles of known chemical composition with elevated heavy metals/metalloids, notably arsenic, using an in vitro cell-free DNA plasmid scission assay. These samples were incubated with and without H
2
O
2
to see whether damage occurs directly or indirectly through the Fenton reaction. Levels of DNA damage in the absence of H
2
O
2
were |
| doi_str_mv | 10.1007/s10653-020-00653-9 |
| format | Article |
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2
O
2
to see whether damage occurs directly or indirectly through the Fenton reaction. Levels of DNA damage in the absence of H
2
O
2
were < 10%, but in the presence of H
2
O
2
, all samples showed higher levels of damage ranging from 10 to 100% suggesting that damage was being incurred through the Fenton reaction. Using bivariate correlation analysis and multiple linear regression, manganese oxide (MnO), sulphur (S), copper (Cu), and zinc (Zn) concentrations in the particulates were found to be the most significant predictors of DNA damage. The mechanism of this DNA damage formation has yet to be thoroughly investigated but is hypothesised to be due to reactive oxygen species formation. Further work is required to assess the extent of contribution of reactive oxygen species to this DNA damage, but this study highlights the potential role of chemistry and/or mineralogy to the extent and/or nature of DNA damage caused by particulates.</description><identifier>ISSN: 0269-4042</identifier><identifier>EISSN: 1573-2983</identifier><identifier>DOI: 10.1007/s10653-020-00653-9</identifier><identifier>PMID: 32648060</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Arsenic ; Arsenic - analysis ; Bivariate analysis ; Chemical composition ; Chromosomes ; Cleavage ; Copper ; Correlation analysis ; Damage ; Deoxyribonucleic acid ; DNA ; DNA - drug effects ; DNA damage ; DNA Damage - drug effects ; Earth and Environmental Science ; Environment ; Environmental Chemistry ; Environmental Health ; Genomic instability ; Geochemistry ; Heavy metals ; Hydrogen peroxide ; Hydrogen Peroxide - analysis ; Manganese ; Manganese oxides ; Metalloids ; Metalloids - analysis ; Metals ; Metals, Heavy - analysis ; Mineralogy ; Minerals - analysis ; Mutation ; Original Paper ; Oxygen ; Particulates ; Plasmids ; Portugal ; Powders ; Public Health ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Soil Science & Conservation ; Sulfur ; Sulphur ; Terrestrial Pollution ; Zinc</subject><ispartof>Environmental geochemistry and health, 2021-07, Vol.43 (7), p.2631-2647</ispartof><rights>The Author(s) 2020</rights><rights>2020. The Author(s).</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-669481827ca0511cb8b82643d38bc15839b22f671298c5596c76e0482ce2486c3</citedby><cites>FETCH-LOGICAL-c474t-669481827ca0511cb8b82643d38bc15839b22f671298c5596c76e0482ce2486c3</cites><orcidid>0000-0002-7484-6696</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10653-020-00653-9$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10653-020-00653-9$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32648060$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Badri, Hatim</creatorcontrib><creatorcontrib>Polya, David A.</creatorcontrib><creatorcontrib>Povey, Andrew. C.</creatorcontrib><title>Geochemical compositional controls on DNA strand breaks induced in in vitro cell-free assays by crushed rock powders from the Panasqueira mine area, Portugal</title><title>Environmental geochemistry and health</title><addtitle>Environ Geochem Health</addtitle><addtitle>Environ Geochem Health</addtitle><description>DNA strand breaks are a common form of DNA damage that can contribute to chromosomal instability or gene mutations. Such strand breaks may be caused by exposure to heavy metals. The aim of this study was to assess the level of DNA strand breaks caused by µm-scale solid particles of known chemical composition with elevated heavy metals/metalloids, notably arsenic, using an in vitro cell-free DNA plasmid scission assay. These samples were incubated with and without H
2
O
2
to see whether damage occurs directly or indirectly through the Fenton reaction. Levels of DNA damage in the absence of H
2
O
2
were < 10%, but in the presence of H
2
O
2
, all samples showed higher levels of damage ranging from 10 to 100% suggesting that damage was being incurred through the Fenton reaction. Using bivariate correlation analysis and multiple linear regression, manganese oxide (MnO), sulphur (S), copper (Cu), and zinc (Zn) concentrations in the particulates were found to be the most significant predictors of DNA damage. The mechanism of this DNA damage formation has yet to be thoroughly investigated but is hypothesised to be due to reactive oxygen species formation. Further work is required to assess the extent of contribution of reactive oxygen species to this DNA damage, but this study highlights the potential role of chemistry and/or mineralogy to the extent and/or nature of DNA damage caused by particulates.</description><subject>Arsenic</subject><subject>Arsenic - analysis</subject><subject>Bivariate analysis</subject><subject>Chemical composition</subject><subject>Chromosomes</subject><subject>Cleavage</subject><subject>Copper</subject><subject>Correlation analysis</subject><subject>Damage</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - drug effects</subject><subject>DNA damage</subject><subject>DNA Damage - drug effects</subject><subject>Earth and Environmental Science</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Genomic instability</subject><subject>Geochemistry</subject><subject>Heavy metals</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - analysis</subject><subject>Manganese</subject><subject>Manganese oxides</subject><subject>Metalloids</subject><subject>Metalloids - analysis</subject><subject>Metals</subject><subject>Metals, Heavy - analysis</subject><subject>Mineralogy</subject><subject>Minerals - analysis</subject><subject>Mutation</subject><subject>Original Paper</subject><subject>Oxygen</subject><subject>Particulates</subject><subject>Plasmids</subject><subject>Portugal</subject><subject>Powders</subject><subject>Public Health</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Soil Science & Conservation</subject><subject>Sulfur</subject><subject>Sulphur</subject><subject>Terrestrial Pollution</subject><subject>Zinc</subject><issn>0269-4042</issn><issn>1573-2983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9UU1v1DAQtRCILoU_wAFZ4kpg_BnnglQVKEgV9ABny3GcXbdJvHiSov0x_Fe8u6XABcnS2Jo37_nNI-Q5g9cMoH6DDLQSFXCo4HBrHpAVU7WoeGPEQ7ICrptKguQn5AniNQA0tTSPyYngWhrQsCI_L0LymzBG7wbq07hNGOeYpsNrmnMakKaJvvt8RnHObupom4O7QRqnbvGhK3V_bmOBUh-GoepzCNQhuh3Sdkd9XnBTcDn5G7pNP7qQkfY5jXTeBHrlJofflxCzo2OcymBhf0WvUp6XtRuekke9GzA8u6un5NuH91_PP1aXXy4-nZ9dVl7Wcq60bqRhhtfegWLMt6Y1xaHohGk9U0Y0Lee9rlnZi1eq0b7WAaThPnBptBen5O2Rd7u0Y-h8KM7dYLc5ji7vbHLR_tuZ4sau060tmkrJuhC8vCPIqdjB2V6nJZctouVKgdJKwh7FjyifE2IO_b0CA7uP1B4jtSVSe4jUNmXoxd9_ux_5nWEBiCMAS2tah_xH-z-0vwBSoK7D</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Badri, Hatim</creator><creator>Polya, David A.</creator><creator>Povey, Andrew. C.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>SOI</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-7484-6696</orcidid></search><sort><creationdate>20210701</creationdate><title>Geochemical compositional controls on DNA strand breaks induced in in vitro cell-free assays by crushed rock powders from the Panasqueira mine area, Portugal</title><author>Badri, Hatim ; Polya, David A. ; Povey, Andrew. 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C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Geochemical compositional controls on DNA strand breaks induced in in vitro cell-free assays by crushed rock powders from the Panasqueira mine area, Portugal</atitle><jtitle>Environmental geochemistry and health</jtitle><stitle>Environ Geochem Health</stitle><addtitle>Environ Geochem Health</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>43</volume><issue>7</issue><spage>2631</spage><epage>2647</epage><pages>2631-2647</pages><issn>0269-4042</issn><eissn>1573-2983</eissn><abstract>DNA strand breaks are a common form of DNA damage that can contribute to chromosomal instability or gene mutations. Such strand breaks may be caused by exposure to heavy metals. The aim of this study was to assess the level of DNA strand breaks caused by µm-scale solid particles of known chemical composition with elevated heavy metals/metalloids, notably arsenic, using an in vitro cell-free DNA plasmid scission assay. These samples were incubated with and without H
2
O
2
to see whether damage occurs directly or indirectly through the Fenton reaction. Levels of DNA damage in the absence of H
2
O
2
were < 10%, but in the presence of H
2
O
2
, all samples showed higher levels of damage ranging from 10 to 100% suggesting that damage was being incurred through the Fenton reaction. Using bivariate correlation analysis and multiple linear regression, manganese oxide (MnO), sulphur (S), copper (Cu), and zinc (Zn) concentrations in the particulates were found to be the most significant predictors of DNA damage. The mechanism of this DNA damage formation has yet to be thoroughly investigated but is hypothesised to be due to reactive oxygen species formation. Further work is required to assess the extent of contribution of reactive oxygen species to this DNA damage, but this study highlights the potential role of chemistry and/or mineralogy to the extent and/or nature of DNA damage caused by particulates.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>32648060</pmid><doi>10.1007/s10653-020-00653-9</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0002-7484-6696</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Environmental geochemistry and health, 2021-07, Vol.43 (7), p.2631-2647 |
| issn | 0269-4042 1573-2983 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Arsenic Arsenic - analysis Bivariate analysis Chemical composition Chromosomes Cleavage Copper Correlation analysis Damage Deoxyribonucleic acid DNA DNA - drug effects DNA damage DNA Damage - drug effects Earth and Environmental Science Environment Environmental Chemistry Environmental Health Genomic instability Geochemistry Heavy metals Hydrogen peroxide Hydrogen Peroxide - analysis Manganese Manganese oxides Metalloids Metalloids - analysis Metals Metals, Heavy - analysis Mineralogy Minerals - analysis Mutation Original Paper Oxygen Particulates Plasmids Portugal Powders Public Health Reactive oxygen species Reactive Oxygen Species - metabolism Soil Science & Conservation Sulfur Sulphur Terrestrial Pollution Zinc |
| title | Geochemical compositional controls on DNA strand breaks induced in in vitro cell-free assays by crushed rock powders from the Panasqueira mine area, Portugal |
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