Assessment of the Bacterial Pollution and Detection of Antibiotic Resistance Genes in Benin: Case of the Hydrographic Channel Complex Cotonou-Nokoué Lake
The study aims to document the level of contamination of the aquatic ecosystem of the Cotonou-Lake Nokoué canal hydrographic complex by multidrug-resistant bacteria and their resistance genes. For this purpose, water samples were taken from several points of the complex and from the sediments at the...
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| Veröffentlicht in: | Journal of environmental and public health 2021, Vol.2021, p.1-8 |
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| creator | Ichola, Oloufemi Daniel Dougnon, Victorien Tamegnon Koudokpon, Charles Hornel Agbankpe, Alidehou Jerrold Deguenon, Esther Ayena, Aime Cezaire Soclo, Henri Houenoukpo |
| description | The study aims to document the level of contamination of the aquatic ecosystem of the Cotonou-Lake Nokoué canal hydrographic complex by multidrug-resistant bacteria and their resistance genes. For this purpose, water samples were taken from several points of the complex and from the sediments at the depth of the lake. Samples of several species of freshly caught fish products from the lake were also collected. Bacteriological analyses were carried out according to the AFNOR standard (NF U: 47–100). The identification of the different bacterial species isolated was then carried out using the API 20E gallery and specific biochemical tests. The antibiogram of the strains was performed according to the recommendations of the EUCAST. Molecular characterization of the identified strains was carried out by searching for resistance and virulence genes. The results obtained revealed the presence of several bacterial species in water samples and in sediment and intestine samples of fishery products with a predominance of Gram-negative bacilli. The resistance profile of Gram-negative bacilli showed a total resistance to metronidazole (100%). 23% of the strains were also resistant to ciprofloxacin, 41% to amoxicillin, and 60% to aztreonam. Of the Gram-positive cocci identified, 66% was resistant to vancomycin, 7.5% to ciprofloxacin, 71% to erythromycin, and 22% to tetracycline. Regarding the genes sought, blaTEM (46%), blaSHV (24%), and blaCTX-M-15 (31%) were present in the genome of Gram-negative bacilli as resistance genes and fimH (41%) as virulence gene. As for Gram-positive cocci, the van B gene was completely absent. The van A was present at 6.25% in Staphylococcus aureus and mecA at 21.88 and 33.33%, respectively, in Staphylococcus aureus and coagulase-negative staphylococci strains. The high resistance of isolated bacterial strains is a matter of concern and calls for a rational use of antibiotics in order to avoid the transmission of antibiotic resistance from the environment to humans. |
| doi_str_mv | 10.1155/2021/6664816 |
| format | Article |
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For this purpose, water samples were taken from several points of the complex and from the sediments at the depth of the lake. Samples of several species of freshly caught fish products from the lake were also collected. Bacteriological analyses were carried out according to the AFNOR standard (NF U: 47–100). The identification of the different bacterial species isolated was then carried out using the API 20E gallery and specific biochemical tests. The antibiogram of the strains was performed according to the recommendations of the EUCAST. Molecular characterization of the identified strains was carried out by searching for resistance and virulence genes. The results obtained revealed the presence of several bacterial species in water samples and in sediment and intestine samples of fishery products with a predominance of Gram-negative bacilli. The resistance profile of Gram-negative bacilli showed a total resistance to metronidazole (100%). 23% of the strains were also resistant to ciprofloxacin, 41% to amoxicillin, and 60% to aztreonam. Of the Gram-positive cocci identified, 66% was resistant to vancomycin, 7.5% to ciprofloxacin, 71% to erythromycin, and 22% to tetracycline. Regarding the genes sought, blaTEM (46%), blaSHV (24%), and blaCTX-M-15 (31%) were present in the genome of Gram-negative bacilli as resistance genes and fimH (41%) as virulence gene. As for Gram-positive cocci, the van B gene was completely absent. The van A was present at 6.25% in Staphylococcus aureus and mecA at 21.88 and 33.33%, respectively, in Staphylococcus aureus and coagulase-negative staphylococci strains. The high resistance of isolated bacterial strains is a matter of concern and calls for a rational use of antibiotics in order to avoid the transmission of antibiotic resistance from the environment to humans.</description><identifier>ISSN: 1687-9805</identifier><identifier>EISSN: 1687-9813</identifier><identifier>DOI: 10.1155/2021/6664816</identifier><identifier>PMID: 34285697</identifier><language>eng</language><publisher>New York: Hindawi</publisher><subject>Amoxicillin ; Antibiotic resistance ; Antibiotics ; Antimicrobial agents ; Aquatic ecosystems ; Aztreonam ; Bacilli ; Bacteria ; Bacterial infections ; Biochemical tests ; Ciprofloxacin ; Coagulase ; Cocci ; Contamination ; Developing countries ; Drug resistance ; Drug resistance in microorganisms ; Erythromycin ; Fisheries ; Fishery products ; Genes ; Genomes ; Gram-negative bacilli ; Gram-positive cocci ; High resistance ; Intestine ; Lakes ; LDCs ; Methylene blue ; Metronidazole ; Multidrug resistance ; Multidrug resistant organisms ; Pollution detection ; Public health ; Sediments ; Species ; Staphylococcus aureus ; Strains (organisms) ; Tetracycline ; Tetracyclines ; Vancomycin ; Virulence ; Virulence (Microbiology) ; Water analysis ; Water depth ; Water quality ; Water sampling</subject><ispartof>Journal of environmental and public health, 2021, Vol.2021, p.1-8</ispartof><rights>Copyright © 2021 Oloufemi Daniel Ichola et al.</rights><rights>COPYRIGHT 2021 John Wiley & Sons, Inc.</rights><rights>Copyright © 2021 Oloufemi Daniel Ichola et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Oloufemi Daniel Ichola et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-50aad6a4b7c9a70072967f77825b6ee28036ee5989f06081f21f70fe57f638033</citedby><cites>FETCH-LOGICAL-c481t-50aad6a4b7c9a70072967f77825b6ee28036ee5989f06081f21f70fe57f638033</cites><orcidid>0000-0002-0547-7065 ; 0000-0001-9047-7299</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275426/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275426/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,27923,27924,27925,53791,53793</link.rule.ids></links><search><contributor>Kharat, Arun S.</contributor><contributor>Arun S Kharat</contributor><creatorcontrib>Ichola, Oloufemi Daniel</creatorcontrib><creatorcontrib>Dougnon, Victorien Tamegnon</creatorcontrib><creatorcontrib>Koudokpon, Charles Hornel</creatorcontrib><creatorcontrib>Agbankpe, Alidehou Jerrold</creatorcontrib><creatorcontrib>Deguenon, Esther</creatorcontrib><creatorcontrib>Ayena, Aime Cezaire</creatorcontrib><creatorcontrib>Soclo, Henri Houenoukpo</creatorcontrib><title>Assessment of the Bacterial Pollution and Detection of Antibiotic Resistance Genes in Benin: Case of the Hydrographic Channel Complex Cotonou-Nokoué Lake</title><title>Journal of environmental and public health</title><description>The study aims to document the level of contamination of the aquatic ecosystem of the Cotonou-Lake Nokoué canal hydrographic complex by multidrug-resistant bacteria and their resistance genes. For this purpose, water samples were taken from several points of the complex and from the sediments at the depth of the lake. Samples of several species of freshly caught fish products from the lake were also collected. Bacteriological analyses were carried out according to the AFNOR standard (NF U: 47–100). The identification of the different bacterial species isolated was then carried out using the API 20E gallery and specific biochemical tests. The antibiogram of the strains was performed according to the recommendations of the EUCAST. Molecular characterization of the identified strains was carried out by searching for resistance and virulence genes. The results obtained revealed the presence of several bacterial species in water samples and in sediment and intestine samples of fishery products with a predominance of Gram-negative bacilli. The resistance profile of Gram-negative bacilli showed a total resistance to metronidazole (100%). 23% of the strains were also resistant to ciprofloxacin, 41% to amoxicillin, and 60% to aztreonam. Of the Gram-positive cocci identified, 66% was resistant to vancomycin, 7.5% to ciprofloxacin, 71% to erythromycin, and 22% to tetracycline. Regarding the genes sought, blaTEM (46%), blaSHV (24%), and blaCTX-M-15 (31%) were present in the genome of Gram-negative bacilli as resistance genes and fimH (41%) as virulence gene. As for Gram-positive cocci, the van B gene was completely absent. The van A was present at 6.25% in Staphylococcus aureus and mecA at 21.88 and 33.33%, respectively, in Staphylococcus aureus and coagulase-negative staphylococci strains. The high resistance of isolated bacterial strains is a matter of concern and calls for a rational use of antibiotics in order to avoid the transmission of antibiotic resistance from the environment to humans.</description><subject>Amoxicillin</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Aquatic ecosystems</subject><subject>Aztreonam</subject><subject>Bacilli</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Biochemical tests</subject><subject>Ciprofloxacin</subject><subject>Coagulase</subject><subject>Cocci</subject><subject>Contamination</subject><subject>Developing countries</subject><subject>Drug resistance</subject><subject>Drug resistance in microorganisms</subject><subject>Erythromycin</subject><subject>Fisheries</subject><subject>Fishery products</subject><subject>Genes</subject><subject>Genomes</subject><subject>Gram-negative 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of the Bacterial Pollution and Detection of Antibiotic Resistance Genes in Benin: Case of the Hydrographic Channel Complex Cotonou-Nokoué Lake</title><author>Ichola, Oloufemi Daniel ; Dougnon, Victorien Tamegnon ; Koudokpon, Charles Hornel ; Agbankpe, Alidehou Jerrold ; Deguenon, Esther ; Ayena, Aime Cezaire ; Soclo, Henri Houenoukpo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-50aad6a4b7c9a70072967f77825b6ee28036ee5989f06081f21f70fe57f638033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Amoxicillin</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Aquatic ecosystems</topic><topic>Aztreonam</topic><topic>Bacilli</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Biochemical 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Kharat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of the Bacterial Pollution and Detection of Antibiotic Resistance Genes in Benin: Case of the Hydrographic Channel Complex Cotonou-Nokoué Lake</atitle><jtitle>Journal of environmental and public health</jtitle><date>2021</date><risdate>2021</risdate><volume>2021</volume><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>1687-9805</issn><eissn>1687-9813</eissn><abstract>The study aims to document the level of contamination of the aquatic ecosystem of the Cotonou-Lake Nokoué canal hydrographic complex by multidrug-resistant bacteria and their resistance genes. For this purpose, water samples were taken from several points of the complex and from the sediments at the depth of the lake. Samples of several species of freshly caught fish products from the lake were also collected. Bacteriological analyses were carried out according to the AFNOR standard (NF U: 47–100). The identification of the different bacterial species isolated was then carried out using the API 20E gallery and specific biochemical tests. The antibiogram of the strains was performed according to the recommendations of the EUCAST. Molecular characterization of the identified strains was carried out by searching for resistance and virulence genes. The results obtained revealed the presence of several bacterial species in water samples and in sediment and intestine samples of fishery products with a predominance of Gram-negative bacilli. The resistance profile of Gram-negative bacilli showed a total resistance to metronidazole (100%). 23% of the strains were also resistant to ciprofloxacin, 41% to amoxicillin, and 60% to aztreonam. Of the Gram-positive cocci identified, 66% was resistant to vancomycin, 7.5% to ciprofloxacin, 71% to erythromycin, and 22% to tetracycline. Regarding the genes sought, blaTEM (46%), blaSHV (24%), and blaCTX-M-15 (31%) were present in the genome of Gram-negative bacilli as resistance genes and fimH (41%) as virulence gene. As for Gram-positive cocci, the van B gene was completely absent. The van A was present at 6.25% in Staphylococcus aureus and mecA at 21.88 and 33.33%, respectively, in Staphylococcus aureus and coagulase-negative staphylococci strains. The high resistance of isolated bacterial strains is a matter of concern and calls for a rational use of antibiotics in order to avoid the transmission of antibiotic resistance from the environment to humans.</abstract><cop>New York</cop><pub>Hindawi</pub><pmid>34285697</pmid><doi>10.1155/2021/6664816</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-0547-7065</orcidid><orcidid>https://orcid.org/0000-0001-9047-7299</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1687-9805 |
| ispartof | Journal of environmental and public health, 2021, Vol.2021, p.1-8 |
| issn | 1687-9805 1687-9813 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8275426 |
| source | PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central; Alma/SFX Local Collection |
| subjects | Amoxicillin Antibiotic resistance Antibiotics Antimicrobial agents Aquatic ecosystems Aztreonam Bacilli Bacteria Bacterial infections Biochemical tests Ciprofloxacin Coagulase Cocci Contamination Developing countries Drug resistance Drug resistance in microorganisms Erythromycin Fisheries Fishery products Genes Genomes Gram-negative bacilli Gram-positive cocci High resistance Intestine Lakes LDCs Methylene blue Metronidazole Multidrug resistance Multidrug resistant organisms Pollution detection Public health Sediments Species Staphylococcus aureus Strains (organisms) Tetracycline Tetracyclines Vancomycin Virulence Virulence (Microbiology) Water analysis Water depth Water quality Water sampling |
| title | Assessment of the Bacterial Pollution and Detection of Antibiotic Resistance Genes in Benin: Case of the Hydrographic Channel Complex Cotonou-Nokoué Lake |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T13%3A55%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Assessment%20of%20the%20Bacterial%20Pollution%20and%20Detection%20of%20Antibiotic%20Resistance%20Genes%20in%20Benin:%20Case%20of%20the%20Hydrographic%20Channel%20Complex%20Cotonou-Nokou%C3%A9%20Lake&rft.jtitle=Journal%20of%20environmental%20and%20public%20health&rft.au=Ichola,%20Oloufemi%20Daniel&rft.date=2021&rft.volume=2021&rft.spage=1&rft.epage=8&rft.pages=1-8&rft.issn=1687-9805&rft.eissn=1687-9813&rft_id=info:doi/10.1155/2021/6664816&rft_dat=%3Cgale_pubme%3EA696897975%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2552745107&rft_id=info:pmid/34285697&rft_galeid=A696897975&rfr_iscdi=true |