Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae
Objectives To explore Saccharomyces cerevisiae as an expression platform for dengue oral immune complex vaccine development. Results Molecular engineering was applied to create a fusion gene construct (scEDIII-PIGS) consisting of a yeast codon optimized sequence encoding for a synthetic consensus de...
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| Veröffentlicht in: | Biotechnology letters 2021-09, Vol.43 (9), p.1895-1904 |
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| container_title | Biotechnology letters |
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| creator | So, Kum-Kang Chun, Jeesun Luong, Nguyen Ngoc Seo, Hee-Won Kim, Dae-Hyuk |
| description | Objectives
To explore
Saccharomyces cerevisiae
as an expression platform for dengue oral immune complex vaccine development.
Results
Molecular engineering was applied to create a fusion gene construct (scEDIII-PIGS) consisting of a yeast codon optimized sequence encoding for a synthetic consensus dengue envelope domain III (scEDIII) followed by a modified IgG Fc domain (PIGS). Northern blot showed transcription of the target gene, with a temporal expression pattern similar to those from previous work. Western blot showed assembly of various immune complexes from monomer to hexamer. Partial purification of scEDIII-PIGS was also attempted to demonstrate the feasibility of yeast system for immune complex vaccine development. Approximately 1 mg of scEDIII-PIGS can be produced from 1 l culture.
Conclusion
This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in
S. cerevisiae
for future application in developing oral and injectable vaccines against various pathogens. |
| doi_str_mv | 10.1007/s10529-021-03161-7 |
| format | Article |
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To explore
Saccharomyces cerevisiae
as an expression platform for dengue oral immune complex vaccine development.
Results
Molecular engineering was applied to create a fusion gene construct (scEDIII-PIGS) consisting of a yeast codon optimized sequence encoding for a synthetic consensus dengue envelope domain III (scEDIII) followed by a modified IgG Fc domain (PIGS). Northern blot showed transcription of the target gene, with a temporal expression pattern similar to those from previous work. Western blot showed assembly of various immune complexes from monomer to hexamer. Partial purification of scEDIII-PIGS was also attempted to demonstrate the feasibility of yeast system for immune complex vaccine development. Approximately 1 mg of scEDIII-PIGS can be produced from 1 l culture.
Conclusion
This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in
S. cerevisiae
for future application in developing oral and injectable vaccines against various pathogens.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-021-03161-7</identifier><identifier>PMID: 34245387</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antigen-antibody complexes ; Applied Microbiology ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Consensus Sequence ; Conserved sequence ; Dengue fever ; Dengue Vaccines - genetics ; Dengue Vaccines - metabolism ; Dengue Virus - genetics ; Domains ; Fusion protein ; Gene expression ; Immunoglobulin Fc Fragments - genetics ; Immunoglobulin G ; Immunoglobulin G - chemistry ; Immunoglobulin G - genetics ; Life Sciences ; Microbiology ; Original Research Paper ; Protein Domains ; Protein Engineering ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Swine ; Transcription ; Vaccine Development ; Vaccines ; Vector-borne diseases ; Viral Envelope Proteins - chemistry ; Viral Envelope Proteins - genetics ; Yeast ; Yeasts</subject><ispartof>Biotechnology letters, 2021-09, Vol.43 (9), p.1895-1904</ispartof><rights>The Author(s) 2021</rights><rights>2021. The Author(s).</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-75e0f799c3d1cfb69ad152354b08023fa5695888b8dadbcb0b01d5ec2344b8a53</citedby><cites>FETCH-LOGICAL-c474t-75e0f799c3d1cfb69ad152354b08023fa5695888b8dadbcb0b01d5ec2344b8a53</cites><orcidid>0000-0002-9948-5313</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-021-03161-7$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-021-03161-7$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34245387$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>So, Kum-Kang</creatorcontrib><creatorcontrib>Chun, Jeesun</creatorcontrib><creatorcontrib>Luong, Nguyen Ngoc</creatorcontrib><creatorcontrib>Seo, Hee-Won</creatorcontrib><creatorcontrib>Kim, Dae-Hyuk</creatorcontrib><title>Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Objectives
To explore
Saccharomyces cerevisiae
as an expression platform for dengue oral immune complex vaccine development.
Results
Molecular engineering was applied to create a fusion gene construct (scEDIII-PIGS) consisting of a yeast codon optimized sequence encoding for a synthetic consensus dengue envelope domain III (scEDIII) followed by a modified IgG Fc domain (PIGS). Northern blot showed transcription of the target gene, with a temporal expression pattern similar to those from previous work. Western blot showed assembly of various immune complexes from monomer to hexamer. Partial purification of scEDIII-PIGS was also attempted to demonstrate the feasibility of yeast system for immune complex vaccine development. Approximately 1 mg of scEDIII-PIGS can be produced from 1 l culture.
Conclusion
This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in
S. cerevisiae
for future application in developing oral and injectable vaccines against various pathogens.</description><subject>Antigen-antibody complexes</subject><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Consensus Sequence</subject><subject>Conserved sequence</subject><subject>Dengue fever</subject><subject>Dengue Vaccines - genetics</subject><subject>Dengue Vaccines - metabolism</subject><subject>Dengue Virus - genetics</subject><subject>Domains</subject><subject>Fusion protein</subject><subject>Gene expression</subject><subject>Immunoglobulin Fc Fragments - genetics</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - chemistry</subject><subject>Immunoglobulin G - genetics</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Original Research Paper</subject><subject>Protein Domains</subject><subject>Protein Engineering</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Swine</subject><subject>Transcription</subject><subject>Vaccine Development</subject><subject>Vaccines</subject><subject>Vector-borne diseases</subject><subject>Viral Envelope Proteins - chemistry</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kctu1DAUhi0EokPhBVggS2zYBHyNnQ0SqloYqRILYG05zknGVWIHO5m2L8Bz4-mUclmwOov_O5999CP0kpK3lBD1LlMiWVMRRivCaU0r9QhtqFS8qpWqH6MNoYJWUjTsBD3L-YoQ0iiinqITLpiQXKsN-nF-MyfI2ceAY49twH6a1hBdnOYRbrCLIfu8-DAc4guH-2SHCcKC-zVDh6_9ssP2DoOQ14w7CMMKGMIexjgD7uJkfcDb7RaX8cU6t7MpTrcOMnaQYO-zt_AcPentmOHF_TxF3y7Ov559qi4_f9yefbisnFBiqZQE0qumcbyjrm_rxnZUMi5FSzRhvLeybqTWutWd7VrXkpbQToJjXIhWW8lP0fujd17bCTpXDkl2NHPyk023Jlpv_k6C35kh7o1miglRF8Gbe0GK31fIi5l8djCONkBcs2FSElZrxVRBX_-DXsU1hXJeoWrJSkf6IGRHyqWYc4L-4TOUmEPN5lizKTWbu5rNQf3qzzMeVn71WgB-BHKJwgDp99v_0f4EREe2GQ</recordid><startdate>20210901</startdate><enddate>20210901</enddate><creator>So, Kum-Kang</creator><creator>Chun, Jeesun</creator><creator>Luong, Nguyen Ngoc</creator><creator>Seo, Hee-Won</creator><creator>Kim, Dae-Hyuk</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9948-5313</orcidid></search><sort><creationdate>20210901</creationdate><title>Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae</title><author>So, Kum-Kang ; Chun, Jeesun ; Luong, Nguyen Ngoc ; Seo, Hee-Won ; Kim, Dae-Hyuk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-75e0f799c3d1cfb69ad152354b08023fa5695888b8dadbcb0b01d5ec2344b8a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antigen-antibody complexes</topic><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Consensus Sequence</topic><topic>Conserved sequence</topic><topic>Dengue fever</topic><topic>Dengue Vaccines - genetics</topic><topic>Dengue Vaccines - metabolism</topic><topic>Dengue Virus - genetics</topic><topic>Domains</topic><topic>Fusion protein</topic><topic>Gene expression</topic><topic>Immunoglobulin Fc Fragments - genetics</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - chemistry</topic><topic>Immunoglobulin G - genetics</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Original Research Paper</topic><topic>Protein Domains</topic><topic>Protein Engineering</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Swine</topic><topic>Transcription</topic><topic>Vaccine Development</topic><topic>Vaccines</topic><topic>Vector-borne diseases</topic><topic>Viral Envelope Proteins - chemistry</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>So, Kum-Kang</creatorcontrib><creatorcontrib>Chun, Jeesun</creatorcontrib><creatorcontrib>Luong, Nguyen Ngoc</creatorcontrib><creatorcontrib>Seo, Hee-Won</creatorcontrib><creatorcontrib>Kim, Dae-Hyuk</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>So, Kum-Kang</au><au>Chun, Jeesun</au><au>Luong, Nguyen Ngoc</au><au>Seo, Hee-Won</au><au>Kim, Dae-Hyuk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2021-09-01</date><risdate>2021</risdate><volume>43</volume><issue>9</issue><spage>1895</spage><epage>1904</epage><pages>1895-1904</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>Objectives
To explore
Saccharomyces cerevisiae
as an expression platform for dengue oral immune complex vaccine development.
Results
Molecular engineering was applied to create a fusion gene construct (scEDIII-PIGS) consisting of a yeast codon optimized sequence encoding for a synthetic consensus dengue envelope domain III (scEDIII) followed by a modified IgG Fc domain (PIGS). Northern blot showed transcription of the target gene, with a temporal expression pattern similar to those from previous work. Western blot showed assembly of various immune complexes from monomer to hexamer. Partial purification of scEDIII-PIGS was also attempted to demonstrate the feasibility of yeast system for immune complex vaccine development. Approximately 1 mg of scEDIII-PIGS can be produced from 1 l culture.
Conclusion
This work demonstrated for the first time that various immunocomplex structures of our target protein could be efficiently produced in
S. cerevisiae
for future application in developing oral and injectable vaccines against various pathogens.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>34245387</pmid><doi>10.1007/s10529-021-03161-7</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-9948-5313</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Biotechnology letters, 2021-09, Vol.43 (9), p.1895-1904 |
| issn | 0141-5492 1573-6776 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Antigen-antibody complexes Applied Microbiology Biochemistry Biomedical and Life Sciences Biotechnology Consensus Sequence Conserved sequence Dengue fever Dengue Vaccines - genetics Dengue Vaccines - metabolism Dengue Virus - genetics Domains Fusion protein Gene expression Immunoglobulin Fc Fragments - genetics Immunoglobulin G Immunoglobulin G - chemistry Immunoglobulin G - genetics Life Sciences Microbiology Original Research Paper Protein Domains Protein Engineering Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Swine Transcription Vaccine Development Vaccines Vector-borne diseases Viral Envelope Proteins - chemistry Viral Envelope Proteins - genetics Yeast Yeasts |
| title | Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae |
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