Effect of combined colistin and meropenem against meropenem resistant Acinetobacter baumannii and Pseudomonas aeruginosa by checkerboard method: A cross sectional analytical study
BACKGROUND: Meropenem-resistant Acinetobacter baumannii and Pseudomonas aeruginosa are the two most common nosocomial pathogens causing ventilator-associated pneumonia. To combat this resistance, different combinations of antibiotics have been evaluated for their efficacy in laboratories as well as...
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creator | Gunalan, Anitha Sarumathi, Dhandapani Sastry, Apurba Ramanathan, Venkateswaran Rajaa, Sathish Sistla, Sujatha |
description | BACKGROUND: Meropenem-resistant Acinetobacter baumannii and Pseudomonas aeruginosa are the two most common nosocomial pathogens causing ventilator-associated pneumonia. To combat this resistance, different combinations of antibiotics have been evaluated for their efficacy in laboratories as well as in clinical situations.
AIM: The aim of the study was to investigate the effect of combined colistin and meropenem against meropenem-resistant isolates of A. baumannii and P. aeruginosa by checkerboard method.
MATERIALS AND METHODS: Fifty meropenem-resistant isolates of A. baumannii (n = 25) and P. aeruginosa (n = 25) from endotracheal aspirates were studied. The MIC of colistin and meropenem was found using the microbroth dilution method. The fractional inhibitory concentration was calculated for the combination of antibiotics by checkerboard assay and the antibiotic interactions were assessed. Fisher's exact test was carried out for statistical comparison of categorical variables.
RESULTS: A synergistic effect between colistin and meropenem was observed in 18/25 (72%) and 6/25 (24%) isolates of Acinetobacter baumannnii and P. Aeruginosa, respectively, with fractional inhibitory concentration indices of ≤0.5. None of the tested isolates exhibited antagonism.
CONCLUSION: Our results showed that combinations of colistin and meropenem are associated with improvement in minimum inhibitory concentration and may be a promising strategy in treating meropenem-resistant A. baumannii respiratory tract infections. |
doi_str_mv | 10.4103/ijp.ijp_1013_20 |
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AIM: The aim of the study was to investigate the effect of combined colistin and meropenem against meropenem-resistant isolates of A. baumannii and P. aeruginosa by checkerboard method.
MATERIALS AND METHODS: Fifty meropenem-resistant isolates of A. baumannii (n = 25) and P. aeruginosa (n = 25) from endotracheal aspirates were studied. The MIC of colistin and meropenem was found using the microbroth dilution method. The fractional inhibitory concentration was calculated for the combination of antibiotics by checkerboard assay and the antibiotic interactions were assessed. Fisher's exact test was carried out for statistical comparison of categorical variables.
RESULTS: A synergistic effect between colistin and meropenem was observed in 18/25 (72%) and 6/25 (24%) isolates of Acinetobacter baumannnii and P. Aeruginosa, respectively, with fractional inhibitory concentration indices of ≤0.5. None of the tested isolates exhibited antagonism.
CONCLUSION: Our results showed that combinations of colistin and meropenem are associated with improvement in minimum inhibitory concentration and may be a promising strategy in treating meropenem-resistant A. baumannii respiratory tract infections.</description><identifier>ISSN: 0253-7613</identifier><identifier>EISSN: 1998-3751</identifier><identifier>DOI: 10.4103/ijp.ijp_1013_20</identifier><identifier>PMID: 34169905</identifier><language>eng</language><publisher>India: Wolters Kluwer India Pvt. Ltd</publisher><subject>Acinetobacter baumannii ; Acinetobacter baumannii - drug effects ; Analysis ; Anti-Bacterial Agents - administration & dosage ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Colistin ; Colistin - administration & dosage ; Colistin - pharmacology ; Cross-Sectional Studies ; Drug Combinations ; Drug resistance in microorganisms ; Drug Resistance, Multiple, Bacterial - drug effects ; Drug Synergism ; Humans ; Identification and classification ; Meropenem - administration & dosage ; Meropenem - pharmacology ; Microbial Sensitivity Tests ; Physiological aspects ; Properties ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects</subject><ispartof>Indian journal of pharmacology, 2021-05, Vol.53 (3), p.207-212</ispartof><rights>COPYRIGHT 2021 Medknow Publications and Media Pvt. Ltd.</rights><rights>2021. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright: © 2021 Indian Journal of Pharmacology 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262418/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262418/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34169905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gunalan, Anitha</creatorcontrib><creatorcontrib>Sarumathi, Dhandapani</creatorcontrib><creatorcontrib>Sastry, Apurba</creatorcontrib><creatorcontrib>Ramanathan, Venkateswaran</creatorcontrib><creatorcontrib>Rajaa, Sathish</creatorcontrib><creatorcontrib>Sistla, Sujatha</creatorcontrib><title>Effect of combined colistin and meropenem against meropenem resistant Acinetobacter baumannii and Pseudomonas aeruginosa by checkerboard method: A cross sectional analytical study</title><title>Indian journal of pharmacology</title><addtitle>Indian J Pharmacol</addtitle><description>BACKGROUND: Meropenem-resistant Acinetobacter baumannii and Pseudomonas aeruginosa are the two most common nosocomial pathogens causing ventilator-associated pneumonia. To combat this resistance, different combinations of antibiotics have been evaluated for their efficacy in laboratories as well as in clinical situations.
AIM: The aim of the study was to investigate the effect of combined colistin and meropenem against meropenem-resistant isolates of A. baumannii and P. aeruginosa by checkerboard method.
MATERIALS AND METHODS: Fifty meropenem-resistant isolates of A. baumannii (n = 25) and P. aeruginosa (n = 25) from endotracheal aspirates were studied. The MIC of colistin and meropenem was found using the microbroth dilution method. The fractional inhibitory concentration was calculated for the combination of antibiotics by checkerboard assay and the antibiotic interactions were assessed. Fisher's exact test was carried out for statistical comparison of categorical variables.
RESULTS: A synergistic effect between colistin and meropenem was observed in 18/25 (72%) and 6/25 (24%) isolates of Acinetobacter baumannnii and P. Aeruginosa, respectively, with fractional inhibitory concentration indices of ≤0.5. None of the tested isolates exhibited antagonism.
CONCLUSION: Our results showed that combinations of colistin and meropenem are associated with improvement in minimum inhibitory concentration and may be a promising strategy in treating meropenem-resistant A. baumannii respiratory tract infections.</description><subject>Acinetobacter baumannii</subject><subject>Acinetobacter baumannii - drug effects</subject><subject>Analysis</subject><subject>Anti-Bacterial Agents - administration & dosage</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Colistin</subject><subject>Colistin - administration & dosage</subject><subject>Colistin - pharmacology</subject><subject>Cross-Sectional Studies</subject><subject>Drug Combinations</subject><subject>Drug resistance in microorganisms</subject><subject>Drug Resistance, Multiple, Bacterial - drug effects</subject><subject>Drug Synergism</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Meropenem - administration & dosage</subject><subject>Meropenem - pharmacology</subject><subject>Microbial Sensitivity Tests</subject><subject>Physiological aspects</subject><subject>Properties</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><issn>0253-7613</issn><issn>1998-3751</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1ks1v0zAYxiMEYmNw5oYsceHSzh-JY3NAqqbxISbBAc7WG8du3SZ2sZNV_bv4B3HbbXSoKIni2L_neR2_T1G8JnhaEswu3XI9zY8imDBF8ZPinEgpJqyuyNPiHNOKTWpO2FnxIqUlzt-l5M-LM1YSLiWuzovf19YaPaBgkQ5947xp86BzaXAegW9Rb2JYG296BHNwPg1HM9GkDIIf0Exn5RAa0IOJqIGxB--d2zt8T2ZsQx88JAQmjnPnQwLUbJFeGL0ysQkQd4WGRWjfoxnSMaSEUt6Wy6Ium0C3HZzOwzSM7fZl8cxCl8yru_dF8fPj9Y-rz5Obb5--XM1uJrrCdTsBLJuKl0CrRkqQVgsuWsZNVdfCYiqqmvCSlbS0VJeipIJTqLGQuNXSQs3ZRfHh4Lsem9602vghQqfW0fUQtyqAU49XvFuoebhVgnJaEpEN3t0ZxPBrNGlQvUvadB14E8akcjuqSpZkX-vtP-gyjDH_-J7iFRWCsb_UHDqjnLch19U7UzXjnNeUMi4zNTlBzXPL8iaDN9bl6Uf89ASfr9b0Tp8UXB4E-05FYx_OhGC1y6XaZfIol1nx5vgoH_j7IGbg6wHYhC5HKK26cWOiyuzKh83_fPNdq0OCVbDqPsHsDwCs_V0</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Gunalan, Anitha</creator><creator>Sarumathi, Dhandapani</creator><creator>Sastry, Apurba</creator><creator>Ramanathan, Venkateswaran</creator><creator>Rajaa, Sathish</creator><creator>Sistla, Sujatha</creator><general>Wolters Kluwer India Pvt. 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Sarumathi, Dhandapani ; Sastry, Apurba ; Ramanathan, Venkateswaran ; Rajaa, Sathish ; Sistla, Sujatha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507d-a09b564a25b99a9fc868d36e5778f028571643424f2c4842862a70890dc9fa763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acinetobacter baumannii</topic><topic>Acinetobacter baumannii - drug effects</topic><topic>Analysis</topic><topic>Anti-Bacterial Agents - administration & dosage</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Colistin</topic><topic>Colistin - administration & dosage</topic><topic>Colistin - pharmacology</topic><topic>Cross-Sectional Studies</topic><topic>Drug Combinations</topic><topic>Drug resistance in microorganisms</topic><topic>Drug Resistance, Multiple, Bacterial - drug effects</topic><topic>Drug Synergism</topic><topic>Humans</topic><topic>Identification and classification</topic><topic>Meropenem - administration & dosage</topic><topic>Meropenem - pharmacology</topic><topic>Microbial Sensitivity Tests</topic><topic>Physiological aspects</topic><topic>Properties</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gunalan, Anitha</creatorcontrib><creatorcontrib>Sarumathi, Dhandapani</creatorcontrib><creatorcontrib>Sastry, Apurba</creatorcontrib><creatorcontrib>Ramanathan, Venkateswaran</creatorcontrib><creatorcontrib>Rajaa, Sathish</creatorcontrib><creatorcontrib>Sistla, Sujatha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Indian journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gunalan, Anitha</au><au>Sarumathi, Dhandapani</au><au>Sastry, Apurba</au><au>Ramanathan, Venkateswaran</au><au>Rajaa, Sathish</au><au>Sistla, Sujatha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of combined colistin and meropenem against meropenem resistant Acinetobacter baumannii and Pseudomonas aeruginosa by checkerboard method: A cross sectional analytical study</atitle><jtitle>Indian journal of pharmacology</jtitle><addtitle>Indian J Pharmacol</addtitle><date>2021-05-01</date><risdate>2021</risdate><volume>53</volume><issue>3</issue><spage>207</spage><epage>212</epage><pages>207-212</pages><issn>0253-7613</issn><eissn>1998-3751</eissn><abstract>BACKGROUND: Meropenem-resistant Acinetobacter baumannii and Pseudomonas aeruginosa are the two most common nosocomial pathogens causing ventilator-associated pneumonia. To combat this resistance, different combinations of antibiotics have been evaluated for their efficacy in laboratories as well as in clinical situations.
AIM: The aim of the study was to investigate the effect of combined colistin and meropenem against meropenem-resistant isolates of A. baumannii and P. aeruginosa by checkerboard method.
MATERIALS AND METHODS: Fifty meropenem-resistant isolates of A. baumannii (n = 25) and P. aeruginosa (n = 25) from endotracheal aspirates were studied. The MIC of colistin and meropenem was found using the microbroth dilution method. The fractional inhibitory concentration was calculated for the combination of antibiotics by checkerboard assay and the antibiotic interactions were assessed. Fisher's exact test was carried out for statistical comparison of categorical variables.
RESULTS: A synergistic effect between colistin and meropenem was observed in 18/25 (72%) and 6/25 (24%) isolates of Acinetobacter baumannnii and P. Aeruginosa, respectively, with fractional inhibitory concentration indices of ≤0.5. None of the tested isolates exhibited antagonism.
CONCLUSION: Our results showed that combinations of colistin and meropenem are associated with improvement in minimum inhibitory concentration and may be a promising strategy in treating meropenem-resistant A. baumannii respiratory tract infections.</abstract><cop>India</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><pmid>34169905</pmid><doi>10.4103/ijp.ijp_1013_20</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Acinetobacter baumannii Acinetobacter baumannii - drug effects Analysis Anti-Bacterial Agents - administration & dosage Anti-Bacterial Agents - pharmacology Antibiotics Colistin Colistin - administration & dosage Colistin - pharmacology Cross-Sectional Studies Drug Combinations Drug resistance in microorganisms Drug Resistance, Multiple, Bacterial - drug effects Drug Synergism Humans Identification and classification Meropenem - administration & dosage Meropenem - pharmacology Microbial Sensitivity Tests Physiological aspects Properties Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects |
title | Effect of combined colistin and meropenem against meropenem resistant Acinetobacter baumannii and Pseudomonas aeruginosa by checkerboard method: A cross sectional analytical study |
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