SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2 to regulate anthocyanin biosynthesis by activating SlDFR expression in tomato
Anthocyanins play vital roles in plant stress tolerance and growth regulation. Previously, we reported that the photomorphogenesis-related transcription factor SlBBX20 regulates anthocyanin accumulation in tomato. However, the underlying mechanism remains unclear. Here, we showed that SlBBX20 promot...
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| Veröffentlicht in: | Horticulture research 2021-07, Vol.8 (1), Article 163 |
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| creator | Luo, Dan Xiong, Cheng Lin, Aihua Zhang, Chunli Sun, Wenhui Zhang, Junhong Yang, Changxian Lu, Yongen Li, Hanxia Ye, Zhibiao He, Ping Wang, Taotao |
| description | Anthocyanins play vital roles in plant stress tolerance and growth regulation. Previously, we reported that the photomorphogenesis-related transcription factor SlBBX20 regulates anthocyanin accumulation in tomato. However, the underlying mechanism remains unclear. Here, we showed that SlBBX20 promotes anthocyanin biosynthesis by binding the promoter of the anthocyanin biosynthesis gene
SlDFR
, suggesting that SlBBX20 directly activates anthocyanin biosynthesis genes. Furthermore, we found by yeast two-hybrid screening that SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2, and the interaction was confirmed by bimolecular fluorescence complementation and coimmunoprecipitation assays.
SlCSN5
gene silencing led to anthocyanin hyperaccumulation in the transgenic tomato calli and shoots, and
SlCSN5-2
overexpression decreased anthocyanin accumulation, suggesting thSlCSN5-2 enhanced the ubiquitination of SlBBX20 and promoted the degradation of SlBBX20 in vivo. Consistently, silencing the
SlCSN5-2
homolog in tobacco significantly increased the accumulation of the SlBBX20 protein. Since SlBBX20 is a vital regulator of photomorphogenesis, the SlBBX20-SlCSN5-2 module may represent a novel regulatory pathway in light-induced anthocyanin biosynthesis. |
| doi_str_mv | 10.1038/s41438-021-00595-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8245592</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2546785408</sourcerecordid><originalsourceid>FETCH-LOGICAL-c451t-16cb25fc63fa55c20e8dd1bb3d829c3fb4b164fb1b17066c17ef4646e124c4e03</originalsourceid><addsrcrecordid>eNp9kd9qFDEUxoMotrR9Aa8CXkfzfzI3gl1bFYoVV8G7kGQzsymzyZpkqvMKPnWjW1q88eqcw_m-34HzAfCC4FcEM_W6cMKZQpgShLHoBVqegGOKBUUd7eTT1ktJkVQEH4GzUm4wxkRwykT3HBwxTnqmhDgGv9fT-fl3imGI1WfjaoE_Q93CuvVwdf25hyWM0UyppJ2HZbZzDBWup9X6k0AU1gSzH-fJVA9NrNvkFhNDhDaksrTZl1CgXWDjhltTQxyb993lF-h_7bMvJaTYDjfMztR0Cp4NZir-7L6egG-XF19XH9DV9fuPq7dXyHFBKiLSWSoGJ9lghHAUe7XZEGvZRtHescFySyQfLLGkw1I60vmBSy49odxxj9kJeHPg7me78xvnY81m0vscdiYvOpmg_93EsNVjutWKciF62gAv7wE5_Zh9qfomzbl9qWgquOyU4Fg1FT2oXE6lZD88XCBY_4lQHyLULUL9N0K9NBM7mEoTx9HnR_R_XHdjX6Az</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2546785408</pqid></control><display><type>article</type><title>SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2 to regulate anthocyanin biosynthesis by activating SlDFR expression in tomato</title><source>Oxford Journals Open Access Collection</source><source>Nature Free</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Springer Nature OA Free Journals</source><creator>Luo, Dan ; Xiong, Cheng ; Lin, Aihua ; Zhang, Chunli ; Sun, Wenhui ; Zhang, Junhong ; Yang, Changxian ; Lu, Yongen ; Li, Hanxia ; Ye, Zhibiao ; He, Ping ; Wang, Taotao</creator><creatorcontrib>Luo, Dan ; Xiong, Cheng ; Lin, Aihua ; Zhang, Chunli ; Sun, Wenhui ; Zhang, Junhong ; Yang, Changxian ; Lu, Yongen ; Li, Hanxia ; Ye, Zhibiao ; He, Ping ; Wang, Taotao</creatorcontrib><description>Anthocyanins play vital roles in plant stress tolerance and growth regulation. Previously, we reported that the photomorphogenesis-related transcription factor SlBBX20 regulates anthocyanin accumulation in tomato. However, the underlying mechanism remains unclear. Here, we showed that SlBBX20 promotes anthocyanin biosynthesis by binding the promoter of the anthocyanin biosynthesis gene
SlDFR
, suggesting that SlBBX20 directly activates anthocyanin biosynthesis genes. Furthermore, we found by yeast two-hybrid screening that SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2, and the interaction was confirmed by bimolecular fluorescence complementation and coimmunoprecipitation assays.
SlCSN5
gene silencing led to anthocyanin hyperaccumulation in the transgenic tomato calli and shoots, and
SlCSN5-2
overexpression decreased anthocyanin accumulation, suggesting thSlCSN5-2 enhanced the ubiquitination of SlBBX20 and promoted the degradation of SlBBX20 in vivo. Consistently, silencing the
SlCSN5-2
homolog in tobacco significantly increased the accumulation of the SlBBX20 protein. Since SlBBX20 is a vital regulator of photomorphogenesis, the SlBBX20-SlCSN5-2 module may represent a novel regulatory pathway in light-induced anthocyanin biosynthesis.</description><identifier>ISSN: 2662-6810</identifier><identifier>EISSN: 2052-7276</identifier><identifier>DOI: 10.1038/s41438-021-00595-y</identifier><identifier>PMID: 34193855</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/449/1659 ; 631/61/447/2311 ; Accumulation ; Agriculture ; Anthocyanins ; Biomedical and Life Sciences ; Biosynthesis ; Complementation ; Ecology ; Fluorescence ; Gene silencing ; Homology ; Life Sciences ; Photomorphogenesis ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant Sciences ; Plant stress ; Shoots ; Tobacco ; Tomatoes ; Ubiquitination ; Yeasts</subject><ispartof>Horticulture research, 2021-07, Vol.8 (1), Article 163</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-16cb25fc63fa55c20e8dd1bb3d829c3fb4b164fb1b17066c17ef4646e124c4e03</citedby><cites>FETCH-LOGICAL-c451t-16cb25fc63fa55c20e8dd1bb3d829c3fb4b164fb1b17066c17ef4646e124c4e03</cites><orcidid>0000-0002-9602-583X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245592/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245592/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids></links><search><creatorcontrib>Luo, Dan</creatorcontrib><creatorcontrib>Xiong, Cheng</creatorcontrib><creatorcontrib>Lin, Aihua</creatorcontrib><creatorcontrib>Zhang, Chunli</creatorcontrib><creatorcontrib>Sun, Wenhui</creatorcontrib><creatorcontrib>Zhang, Junhong</creatorcontrib><creatorcontrib>Yang, Changxian</creatorcontrib><creatorcontrib>Lu, Yongen</creatorcontrib><creatorcontrib>Li, Hanxia</creatorcontrib><creatorcontrib>Ye, Zhibiao</creatorcontrib><creatorcontrib>He, Ping</creatorcontrib><creatorcontrib>Wang, Taotao</creatorcontrib><title>SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2 to regulate anthocyanin biosynthesis by activating SlDFR expression in tomato</title><title>Horticulture research</title><addtitle>Hortic Res</addtitle><description>Anthocyanins play vital roles in plant stress tolerance and growth regulation. Previously, we reported that the photomorphogenesis-related transcription factor SlBBX20 regulates anthocyanin accumulation in tomato. However, the underlying mechanism remains unclear. Here, we showed that SlBBX20 promotes anthocyanin biosynthesis by binding the promoter of the anthocyanin biosynthesis gene
SlDFR
, suggesting that SlBBX20 directly activates anthocyanin biosynthesis genes. Furthermore, we found by yeast two-hybrid screening that SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2, and the interaction was confirmed by bimolecular fluorescence complementation and coimmunoprecipitation assays.
SlCSN5
gene silencing led to anthocyanin hyperaccumulation in the transgenic tomato calli and shoots, and
SlCSN5-2
overexpression decreased anthocyanin accumulation, suggesting thSlCSN5-2 enhanced the ubiquitination of SlBBX20 and promoted the degradation of SlBBX20 in vivo. Consistently, silencing the
SlCSN5-2
homolog in tobacco significantly increased the accumulation of the SlBBX20 protein. Since SlBBX20 is a vital regulator of photomorphogenesis, the SlBBX20-SlCSN5-2 module may represent a novel regulatory pathway in light-induced anthocyanin biosynthesis.</description><subject>631/449/1659</subject><subject>631/61/447/2311</subject><subject>Accumulation</subject><subject>Agriculture</subject><subject>Anthocyanins</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Complementation</subject><subject>Ecology</subject><subject>Fluorescence</subject><subject>Gene silencing</subject><subject>Homology</subject><subject>Life Sciences</subject><subject>Photomorphogenesis</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Sciences</subject><subject>Plant stress</subject><subject>Shoots</subject><subject>Tobacco</subject><subject>Tomatoes</subject><subject>Ubiquitination</subject><subject>Yeasts</subject><issn>2662-6810</issn><issn>2052-7276</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kd9qFDEUxoMotrR9Aa8CXkfzfzI3gl1bFYoVV8G7kGQzsymzyZpkqvMKPnWjW1q88eqcw_m-34HzAfCC4FcEM_W6cMKZQpgShLHoBVqegGOKBUUd7eTT1ktJkVQEH4GzUm4wxkRwykT3HBwxTnqmhDgGv9fT-fl3imGI1WfjaoE_Q93CuvVwdf25hyWM0UyppJ2HZbZzDBWup9X6k0AU1gSzH-fJVA9NrNvkFhNDhDaksrTZl1CgXWDjhltTQxyb993lF-h_7bMvJaTYDjfMztR0Cp4NZir-7L6egG-XF19XH9DV9fuPq7dXyHFBKiLSWSoGJ9lghHAUe7XZEGvZRtHescFySyQfLLGkw1I60vmBSy49odxxj9kJeHPg7me78xvnY81m0vscdiYvOpmg_93EsNVjutWKciF62gAv7wE5_Zh9qfomzbl9qWgquOyU4Fg1FT2oXE6lZD88XCBY_4lQHyLULUL9N0K9NBM7mEoTx9HnR_R_XHdjX6Az</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Luo, Dan</creator><creator>Xiong, Cheng</creator><creator>Lin, Aihua</creator><creator>Zhang, Chunli</creator><creator>Sun, Wenhui</creator><creator>Zhang, Junhong</creator><creator>Yang, Changxian</creator><creator>Lu, Yongen</creator><creator>Li, Hanxia</creator><creator>Ye, Zhibiao</creator><creator>He, Ping</creator><creator>Wang, Taotao</creator><general>Nature Publishing Group UK</general><general>Oxford University Press</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9602-583X</orcidid></search><sort><creationdate>20210701</creationdate><title>SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2 to regulate anthocyanin biosynthesis by activating SlDFR expression in tomato</title><author>Luo, Dan ; Xiong, Cheng ; Lin, Aihua ; Zhang, Chunli ; Sun, Wenhui ; Zhang, Junhong ; Yang, Changxian ; Lu, Yongen ; Li, Hanxia ; Ye, Zhibiao ; He, Ping ; Wang, Taotao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-16cb25fc63fa55c20e8dd1bb3d829c3fb4b164fb1b17066c17ef4646e124c4e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>631/449/1659</topic><topic>631/61/447/2311</topic><topic>Accumulation</topic><topic>Agriculture</topic><topic>Anthocyanins</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Complementation</topic><topic>Ecology</topic><topic>Fluorescence</topic><topic>Gene silencing</topic><topic>Homology</topic><topic>Life Sciences</topic><topic>Photomorphogenesis</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Sciences</topic><topic>Plant stress</topic><topic>Shoots</topic><topic>Tobacco</topic><topic>Tomatoes</topic><topic>Ubiquitination</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Dan</creatorcontrib><creatorcontrib>Xiong, Cheng</creatorcontrib><creatorcontrib>Lin, Aihua</creatorcontrib><creatorcontrib>Zhang, Chunli</creatorcontrib><creatorcontrib>Sun, Wenhui</creatorcontrib><creatorcontrib>Zhang, Junhong</creatorcontrib><creatorcontrib>Yang, Changxian</creatorcontrib><creatorcontrib>Lu, Yongen</creatorcontrib><creatorcontrib>Li, Hanxia</creatorcontrib><creatorcontrib>Ye, Zhibiao</creatorcontrib><creatorcontrib>He, Ping</creatorcontrib><creatorcontrib>Wang, Taotao</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Horticulture research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Dan</au><au>Xiong, Cheng</au><au>Lin, Aihua</au><au>Zhang, Chunli</au><au>Sun, Wenhui</au><au>Zhang, Junhong</au><au>Yang, Changxian</au><au>Lu, Yongen</au><au>Li, Hanxia</au><au>Ye, Zhibiao</au><au>He, Ping</au><au>Wang, Taotao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2 to regulate anthocyanin biosynthesis by activating SlDFR expression in tomato</atitle><jtitle>Horticulture research</jtitle><stitle>Hortic Res</stitle><date>2021-07-01</date><risdate>2021</risdate><volume>8</volume><issue>1</issue><artnum>163</artnum><issn>2662-6810</issn><eissn>2052-7276</eissn><abstract>Anthocyanins play vital roles in plant stress tolerance and growth regulation. Previously, we reported that the photomorphogenesis-related transcription factor SlBBX20 regulates anthocyanin accumulation in tomato. However, the underlying mechanism remains unclear. Here, we showed that SlBBX20 promotes anthocyanin biosynthesis by binding the promoter of the anthocyanin biosynthesis gene
SlDFR
, suggesting that SlBBX20 directly activates anthocyanin biosynthesis genes. Furthermore, we found by yeast two-hybrid screening that SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2, and the interaction was confirmed by bimolecular fluorescence complementation and coimmunoprecipitation assays.
SlCSN5
gene silencing led to anthocyanin hyperaccumulation in the transgenic tomato calli and shoots, and
SlCSN5-2
overexpression decreased anthocyanin accumulation, suggesting thSlCSN5-2 enhanced the ubiquitination of SlBBX20 and promoted the degradation of SlBBX20 in vivo. Consistently, silencing the
SlCSN5-2
homolog in tobacco significantly increased the accumulation of the SlBBX20 protein. Since SlBBX20 is a vital regulator of photomorphogenesis, the SlBBX20-SlCSN5-2 module may represent a novel regulatory pathway in light-induced anthocyanin biosynthesis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>34193855</pmid><doi>10.1038/s41438-021-00595-y</doi><orcidid>https://orcid.org/0000-0002-9602-583X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 631/449/1659 631/61/447/2311 Accumulation Agriculture Anthocyanins Biomedical and Life Sciences Biosynthesis Complementation Ecology Fluorescence Gene silencing Homology Life Sciences Photomorphogenesis Plant Breeding/Biotechnology Plant Genetics and Genomics Plant Sciences Plant stress Shoots Tobacco Tomatoes Ubiquitination Yeasts |
| title | SlBBX20 interacts with the COP9 signalosome subunit SlCSN5-2 to regulate anthocyanin biosynthesis by activating SlDFR expression in tomato |
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