A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation

•Purification of clade A and C HIV-1 envelope (Env) trimers by a novel non-affinity method.•Env trimers produced in pre-fusion closed conformation without antibody capture.•Non-affinity purified trimers show minimal exposure of CD4-induced or V3 epitopes.•Scalable process yields up to 0.2 g/L of pur...

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Veröffentlicht in:	Vaccine 2021-06, Vol.39 (25), p.3379-3387
Hauptverfasser:	Gulla, Krishana, Cibelli, Nicole, Cooper, Jonathan W., Fuller, Haley C., Schneiderman, Zachary, Witter, Sara, Zhang, Yaqiu, Changela, Anita, Geng, Hui, Hatcher, Christian, Narpala, Sandeep, Tsybovsky, Yaroslav, Zhang, Baoshan, VRC Production Program, McDermott, Adrian B., Kwong, Peter D., Gowetski, Daniel B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity AIDS Vaccines Antibodies Antibodies, Neutralizing Antigens CD4 antigen Chromatography Clinical trials Conformation Cyclic GMP DS-SOSIP env Gene Products, Human Immunodeficiency Virus - genetics Envelope trimer Glycan Glycoprotein Glycoprotein gp120 Glycoproteins GMP manufacturing HIV HIV Antibodies HIV Antigens HIV-1 - genetics HIV-1 vaccine Human immunodeficiency virus Immunization Manufacturing Manufacturing industry Mutation Non-affinity chromatography Peptides Prefusion-closed conformation Protein Multimerization Purification Resins Trimers Vaccines
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