Serial Expression of the Truncated Fragments of the Nucleocapsid Protein of CCHFV and Identification of the Epitope Region
The Crimean-congo hemorrhagic fever virus （CCHFV） is a geographically widespread fatal pathogen Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments of...
Gespeichert in:
| Veröffentlicht in: | Virologica Sinica 2010-02, Vol.25 (1), p.45-51 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 51 |
|---|---|
| container_issue | 1 |
| container_start_page | 45 |
| container_title | Virologica Sinica |
| container_volume | 25 |
| creator | Wei, Peng-fei Luo, Yan-jun Li, Tian-xian Wang, Hua-lin Hu, Zhi-hong Zhang, Fu-chun Zhang, Yu-jiang Deng, Fei Sun, Su-rong |
| description | The Crimean-congo hemorrhagic fever virus （CCHFV） is a geographically widespread fatal pathogen Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments of the NP （ nucleocapsid protein ） gene from the S fragment of CCHFV strain YL04057 were constructed. The recombinant proteins were expressed in E.coli and purified for detection. The antigenic of the truncated fragments of NP was detected with a polyclonal serum （rabbit） and 2 monoclonal （mAbs） （ 14B7 and 43E5 ） against CCHFV by Western-blot analyses The results showed that the three expressed constructs, which all contained the region 235AA to 305AA could be detected by mAbs polyclonal serum. The results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein |
| doi_str_mv | 10.1007/s12250-010-3067-7 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8227947</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>32809277</cqvip_id><wanfj_id>zgbdx201001006</wanfj_id><sourcerecordid>zgbdx201001006</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5396-65a90dad8a2fff504fa577b17f54de1eb372bed47e0a1c762b8353201bcc6a433</originalsourceid><addsrcrecordid>eNp9ksFu1DAQhiMEoqXwAFxQBBK3wNhO7OSChFa7tFIFCAriZjnOOOuStVM7gdKnx8u228IByZJHM9_881ueLHtK4BUBEK8jobSCAggUDLgoxL3skDRNVdQUvt1PMRdlAYI3B9mjGM8BOK0Ze5gdUGg4pPgwu_qMwaohX16OAWO03uXe5NMa87MwO60m7PJVUP0G3RRvSu9nPaDXaoy2yz8GP6H907ZYHK--5sp1-UmXeGtsErgjuRzt5EfMP2Gfso-zB0YNEZ9c30fZl9XybHFcnH54d7J4e1roijW84JVqoFNdragxpoLSqEqIlghTlR0SbJmgLXalQFBEC07bmlWMAmm15qpk7Ch7s9Md53aDnU7OghrkGOxGhV_SKyv_rji7lr3_IWtKRVOKJPByJ_BTOaNcL8_9HFyyLK_6trtMo2B7-C04Bn8xY5zkxkaNw6Ac-jlKSkpS16JO4It_wL0kLYERIjjbUmRH6eBjDGj2ngnI7QLI3QLINF1uF0BuvT67-9h9x82PJ4DugJhKrsdwO_p_qs-vnay96y9Sn2yV_m7sgJLRGhoqBPsN_lPHgg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2403117638</pqid></control><display><type>article</type><title>Serial Expression of the Truncated Fragments of the Nucleocapsid Protein of CCHFV and Identification of the Epitope Region</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Wei, Peng-fei ; Luo, Yan-jun ; Li, Tian-xian ; Wang, Hua-lin ; Hu, Zhi-hong ; Zhang, Fu-chun ; Zhang, Yu-jiang ; Deng, Fei ; Sun, Su-rong</creator><creatorcontrib>Wei, Peng-fei ; Luo, Yan-jun ; Li, Tian-xian ; Wang, Hua-lin ; Hu, Zhi-hong ; Zhang, Fu-chun ; Zhang, Yu-jiang ; Deng, Fei ; Sun, Su-rong</creatorcontrib><description>The Crimean-congo hemorrhagic fever virus （CCHFV） is a geographically widespread fatal pathogen Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments of the NP （ nucleocapsid protein ） gene from the S fragment of CCHFV strain YL04057 were constructed. The recombinant proteins were expressed in E.coli and purified for detection. The antigenic of the truncated fragments of NP was detected with a polyclonal serum （rabbit） and 2 monoclonal （mAbs） （ 14B7 and 43E5 ） against CCHFV by Western-blot analyses The results showed that the three expressed constructs, which all contained the region 235AA to 305AA could be detected by mAbs polyclonal serum. The results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein</description><identifier>ISSN: 1674-0769</identifier><identifier>EISSN: 1995-820X</identifier><identifier>DOI: 10.1007/s12250-010-3067-7</identifier><identifier>PMID: 20960283</identifier><language>eng</language><publisher>Heidelberg: SP Wuhan Institute of Virology, CAS</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antibodies, Viral - immunology ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Blotting, Western ; Crimean hemorrhagic fever ; Crimean-Congo hemorrhagic fever virus ; Epidemiology ; Epitope Mapping - methods ; Epitopes ; Epitopes - genetics ; Epitopes - immunology ; Escherichia coli - genetics ; Expression vectors ; Gene Expression ; Hemorrhage ; Hemorrhagic Fever Virus, Crimean-Congo - genetics ; Hemorrhagic Fever Virus, Crimean-Congo - immunology ; Medical Microbiology ; Microbial Genetics and Genomics ; Microbiology ; Mutant Proteins - genetics ; Mutant Proteins - immunology ; NP protein ; Nucleocapsid Proteins - genetics ; Nucleocapsid Proteins - immunology ; Nucleocapsids ; Oncology ; Proteins ; Rabbits ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Sequence Deletion ; Virology ; 单克隆抗体 ; 抗原表位 ; 核衣壳 ; 衣壳蛋白</subject><ispartof>Virologica Sinica, 2010-02, Vol.25 (1), p.45-51</ispartof><rights>Wuhan Institute of Virology, CAS and Springer Berlin Heidelberg 2010</rights><rights>Wuhan Institute of Virology, CAS and Springer Berlin Heidelberg 2010.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5396-65a90dad8a2fff504fa577b17f54de1eb372bed47e0a1c762b8353201bcc6a433</citedby><cites>FETCH-LOGICAL-c5396-65a90dad8a2fff504fa577b17f54de1eb372bed47e0a1c762b8353201bcc6a433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/92590B/92590B.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227947/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227947/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20960283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, Peng-fei</creatorcontrib><creatorcontrib>Luo, Yan-jun</creatorcontrib><creatorcontrib>Li, Tian-xian</creatorcontrib><creatorcontrib>Wang, Hua-lin</creatorcontrib><creatorcontrib>Hu, Zhi-hong</creatorcontrib><creatorcontrib>Zhang, Fu-chun</creatorcontrib><creatorcontrib>Zhang, Yu-jiang</creatorcontrib><creatorcontrib>Deng, Fei</creatorcontrib><creatorcontrib>Sun, Su-rong</creatorcontrib><title>Serial Expression of the Truncated Fragments of the Nucleocapsid Protein of CCHFV and Identification of the Epitope Region</title><title>Virologica Sinica</title><addtitle>Virol. Sin</addtitle><addtitle>Virologica Sinica</addtitle><description>The Crimean-congo hemorrhagic fever virus （CCHFV） is a geographically widespread fatal pathogen Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments of the NP （ nucleocapsid protein ） gene from the S fragment of CCHFV strain YL04057 were constructed. The recombinant proteins were expressed in E.coli and purified for detection. The antigenic of the truncated fragments of NP was detected with a polyclonal serum （rabbit） and 2 monoclonal （mAbs） （ 14B7 and 43E5 ） against CCHFV by Western-blot analyses The results showed that the three expressed constructs, which all contained the region 235AA to 305AA could be detected by mAbs polyclonal serum. The results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Viral - immunology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blotting, Western</subject><subject>Crimean hemorrhagic fever</subject><subject>Crimean-Congo hemorrhagic fever virus</subject><subject>Epidemiology</subject><subject>Epitope Mapping - methods</subject><subject>Epitopes</subject><subject>Epitopes - genetics</subject><subject>Epitopes - immunology</subject><subject>Escherichia coli - genetics</subject><subject>Expression vectors</subject><subject>Gene Expression</subject><subject>Hemorrhage</subject><subject>Hemorrhagic Fever Virus, Crimean-Congo - genetics</subject><subject>Hemorrhagic Fever Virus, Crimean-Congo - immunology</subject><subject>Medical Microbiology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mutant Proteins - genetics</subject><subject>Mutant Proteins - immunology</subject><subject>NP protein</subject><subject>Nucleocapsid Proteins - genetics</subject><subject>Nucleocapsid Proteins - immunology</subject><subject>Nucleocapsids</subject><subject>Oncology</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Sequence Deletion</subject><subject>Virology</subject><subject>单克隆抗体</subject><subject>抗原表位</subject><subject>核衣壳</subject><subject>衣壳蛋白</subject><issn>1674-0769</issn><issn>1995-820X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9ksFu1DAQhiMEoqXwAFxQBBK3wNhO7OSChFa7tFIFCAriZjnOOOuStVM7gdKnx8u228IByZJHM9_881ueLHtK4BUBEK8jobSCAggUDLgoxL3skDRNVdQUvt1PMRdlAYI3B9mjGM8BOK0Ze5gdUGg4pPgwu_qMwaohX16OAWO03uXe5NMa87MwO60m7PJVUP0G3RRvSu9nPaDXaoy2yz8GP6H907ZYHK--5sp1-UmXeGtsErgjuRzt5EfMP2Gfso-zB0YNEZ9c30fZl9XybHFcnH54d7J4e1roijW84JVqoFNdragxpoLSqEqIlghTlR0SbJmgLXalQFBEC07bmlWMAmm15qpk7Ch7s9Md53aDnU7OghrkGOxGhV_SKyv_rji7lr3_IWtKRVOKJPByJ_BTOaNcL8_9HFyyLK_6trtMo2B7-C04Bn8xY5zkxkaNw6Ac-jlKSkpS16JO4It_wL0kLYERIjjbUmRH6eBjDGj2ngnI7QLI3QLINF1uF0BuvT67-9h9x82PJ4DugJhKrsdwO_p_qs-vnay96y9Sn2yV_m7sgJLRGhoqBPsN_lPHgg</recordid><startdate>201002</startdate><enddate>201002</enddate><creator>Wei, Peng-fei</creator><creator>Luo, Yan-jun</creator><creator>Li, Tian-xian</creator><creator>Wang, Hua-lin</creator><creator>Hu, Zhi-hong</creator><creator>Zhang, Fu-chun</creator><creator>Zhang, Yu-jiang</creator><creator>Deng, Fei</creator><creator>Sun, Su-rong</creator><general>SP Wuhan Institute of Virology, CAS</general><general>KeAi Publishing Communications Ltd</general><general>Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China%State Key Laboratory of Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China%The Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region,Urumqi 830002,China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>201002</creationdate><title>Serial Expression of the Truncated Fragments of the Nucleocapsid Protein of CCHFV and Identification of the Epitope Region</title><author>Wei, Peng-fei ; Luo, Yan-jun ; Li, Tian-xian ; Wang, Hua-lin ; Hu, Zhi-hong ; Zhang, Fu-chun ; Zhang, Yu-jiang ; Deng, Fei ; Sun, Su-rong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5396-65a90dad8a2fff504fa577b17f54de1eb372bed47e0a1c762b8353201bcc6a433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Viral - immunology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blotting, Western</topic><topic>Crimean hemorrhagic fever</topic><topic>Crimean-Congo hemorrhagic fever virus</topic><topic>Epidemiology</topic><topic>Epitope Mapping - methods</topic><topic>Epitopes</topic><topic>Epitopes - genetics</topic><topic>Epitopes - immunology</topic><topic>Escherichia coli - genetics</topic><topic>Expression vectors</topic><topic>Gene Expression</topic><topic>Hemorrhage</topic><topic>Hemorrhagic Fever Virus, Crimean-Congo - genetics</topic><topic>Hemorrhagic Fever Virus, Crimean-Congo - immunology</topic><topic>Medical Microbiology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Mutant Proteins - genetics</topic><topic>Mutant Proteins - immunology</topic><topic>NP protein</topic><topic>Nucleocapsid Proteins - genetics</topic><topic>Nucleocapsid Proteins - immunology</topic><topic>Nucleocapsids</topic><topic>Oncology</topic><topic>Proteins</topic><topic>Rabbits</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Sequence Deletion</topic><topic>Virology</topic><topic>单克隆抗体</topic><topic>抗原表位</topic><topic>核衣壳</topic><topic>衣壳蛋白</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Peng-fei</creatorcontrib><creatorcontrib>Luo, Yan-jun</creatorcontrib><creatorcontrib>Li, Tian-xian</creatorcontrib><creatorcontrib>Wang, Hua-lin</creatorcontrib><creatorcontrib>Hu, Zhi-hong</creatorcontrib><creatorcontrib>Zhang, Fu-chun</creatorcontrib><creatorcontrib>Zhang, Yu-jiang</creatorcontrib><creatorcontrib>Deng, Fei</creatorcontrib><creatorcontrib>Sun, Su-rong</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Peng-fei</au><au>Luo, Yan-jun</au><au>Li, Tian-xian</au><au>Wang, Hua-lin</au><au>Hu, Zhi-hong</au><au>Zhang, Fu-chun</au><au>Zhang, Yu-jiang</au><au>Deng, Fei</au><au>Sun, Su-rong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serial Expression of the Truncated Fragments of the Nucleocapsid Protein of CCHFV and Identification of the Epitope Region</atitle><jtitle>Virologica Sinica</jtitle><stitle>Virol. Sin</stitle><addtitle>Virologica Sinica</addtitle><date>2010-02</date><risdate>2010</risdate><volume>25</volume><issue>1</issue><spage>45</spage><epage>51</epage><pages>45-51</pages><issn>1674-0769</issn><eissn>1995-820X</eissn><abstract>The Crimean-congo hemorrhagic fever virus （CCHFV） is a geographically widespread fatal pathogen Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments of the NP （ nucleocapsid protein ） gene from the S fragment of CCHFV strain YL04057 were constructed. The recombinant proteins were expressed in E.coli and purified for detection. The antigenic of the truncated fragments of NP was detected with a polyclonal serum （rabbit） and 2 monoclonal （mAbs） （ 14B7 and 43E5 ） against CCHFV by Western-blot analyses The results showed that the three expressed constructs, which all contained the region 235AA to 305AA could be detected by mAbs polyclonal serum. The results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein</abstract><cop>Heidelberg</cop><pub>SP Wuhan Institute of Virology, CAS</pub><pmid>20960283</pmid><doi>10.1007/s12250-010-3067-7</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1674-0769 |
| ispartof | Virologica Sinica, 2010-02, Vol.25 (1), p.45-51 |
| issn | 1674-0769 1995-820X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8227947 |
| source | MEDLINE; SpringerLink Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Biochemistry Biomedical and Life Sciences Biomedicine Blotting, Western Crimean hemorrhagic fever Crimean-Congo hemorrhagic fever virus Epidemiology Epitope Mapping - methods Epitopes Epitopes - genetics Epitopes - immunology Escherichia coli - genetics Expression vectors Gene Expression Hemorrhage Hemorrhagic Fever Virus, Crimean-Congo - genetics Hemorrhagic Fever Virus, Crimean-Congo - immunology Medical Microbiology Microbial Genetics and Genomics Microbiology Mutant Proteins - genetics Mutant Proteins - immunology NP protein Nucleocapsid Proteins - genetics Nucleocapsid Proteins - immunology Nucleocapsids Oncology Proteins Rabbits Recombinant Proteins - genetics Recombinant Proteins - immunology Sequence Deletion Virology 单克隆抗体 抗原表位 核衣壳 衣壳蛋白 |
| title | Serial Expression of the Truncated Fragments of the Nucleocapsid Protein of CCHFV and Identification of the Epitope Region |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-16T11%3A24%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Serial%20Expression%20of%20the%20Truncated%20Fragments%20of%20the%20Nucleocapsid%20Protein%20of%20CCHFV%20and%20Identification%20of%20the%20Epitope%20Region&rft.jtitle=Virologica%20Sinica&rft.au=Wei,%20Peng-fei&rft.date=2010-02&rft.volume=25&rft.issue=1&rft.spage=45&rft.epage=51&rft.pages=45-51&rft.issn=1674-0769&rft.eissn=1995-820X&rft_id=info:doi/10.1007/s12250-010-3067-7&rft_dat=%3Cwanfang_jour_pubme%3Ezgbdx201001006%3C/wanfang_jour_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2403117638&rft_id=info:pmid/20960283&rft_cqvip_id=32809277&rft_wanfj_id=zgbdx201001006&rfr_iscdi=true |