Effects of different aperture-sized type I collagen/silk fibroin scaffolds on the proliferation and differentiation of human dental pulp cells
Abstract This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on the proliferation and differentiation of human dental pulp cells (HDPCs). The CSF scaffolds were designed with 3D mapping software Solidworks. Three different aperture-sized...
Gespeichert in:
| Veröffentlicht in: | Regenerative Biomaterials 2021-08, Vol.8 (4), p.1 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 4 |
| container_start_page | 1 |
| container_title | Regenerative Biomaterials |
| container_volume | 8 |
| creator | Jiang, Shihui Yu, Zhaoxia Zhang, Lanrui Wang, Guanhua Dai, Xiaohua Lian, Xiaoli Yan, Yan Zhang, Linpu Wang, Yue Li, Ruixin Zou, Huiru |
| description | Abstract
This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on the proliferation and differentiation of human dental pulp cells (HDPCs). The CSF scaffolds were designed with 3D mapping software Solidworks. Three different aperture-sized scaffolds (CSF1–CSF3) were prepared by low-temperature deposition 3D printing technology. The morphology was observed by scanning electron microscope (SEM) and optical coherence tomography. The porosity, hydrophilicity and mechanical capacity of the scaffold were detected, respectively. HDPCs (third passage, 1 × 105 cells) were seeded into each scaffold and investigated by SEM, CCK-8, alkaline phosphatase (ALP) activity and HE staining. The CSF scaffolds had porous structures with macropores and micropores. The macropore size of CSF1 to CSF3 was 421 ± 27 μm, 579 ± 36 μm and 707 ± 43 μm, respectively. The porosity was 69.8 ± 2.2%, 80.1 ± 2.8% and 86.5 ± 3.3%, respectively. All these scaffolds enhanced the adhesion and proliferation of HDPCs. The ALP activity in the CSF1 group was higher than that in the CSF3 groups (P |
| doi_str_mv | 10.1093/rb/rbab028 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8226109</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A712556140</galeid><oup_id>10.1093/rb/rbab028</oup_id><sourcerecordid>A712556140</sourcerecordid><originalsourceid>FETCH-LOGICAL-c518t-89d0af67a3a97c92ee0a17fcf774927bd4fd7e2a2fef7fbe72e4f6639474c4173</originalsourceid><addsrcrecordid>eNp9kcFq3DAQhkVpacI2lz6BLr0UnEiyLNmXQghpGwjk0p6FLM3sqtVaRrIL6UP0maPFIaWXogENv_75ZhgR8p6zS86G9iqPNezIRP-KnAvWqaaVQr1-yXl_Ri5K-cEY41Irxbu35Owk90Mnz8mfW0RwS6EJqQ81zzAt1M6QlzVDU8Jv8HR5nIHeUZditHuYrkqIPymGMacw0eIsYoq-Iia6HIDOOcVQQXYJVbGT_wsOm1Z7Hdajnaivmo10XuNMHcRY3pE3aGOBi-d7R75_vv1287W5f_hyd3N937iO90vTD55ZVNq2dtBuEADMco0OtZaD0KOX6DUIKxBQ4whagESl2kFq6STX7Y582rjzOh7BuzpHttHMORxtfjTJBvPvyxQOZp9-mV4Iddr7jlxugL2NYMKEqdpcPR6OwaUJMFT9WnPRdYpLVgs-bgUup1Iy4EszzsyJaPJonn-ymj9s5rTO__M9AQxEodk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Effects of different aperture-sized type I collagen/silk fibroin scaffolds on the proliferation and differentiation of human dental pulp cells</title><source>Oxford Journals Open Access Collection</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Jiang, Shihui ; Yu, Zhaoxia ; Zhang, Lanrui ; Wang, Guanhua ; Dai, Xiaohua ; Lian, Xiaoli ; Yan, Yan ; Zhang, Linpu ; Wang, Yue ; Li, Ruixin ; Zou, Huiru</creator><creatorcontrib>Jiang, Shihui ; Yu, Zhaoxia ; Zhang, Lanrui ; Wang, Guanhua ; Dai, Xiaohua ; Lian, Xiaoli ; Yan, Yan ; Zhang, Linpu ; Wang, Yue ; Li, Ruixin ; Zou, Huiru</creatorcontrib><description>Abstract
This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on the proliferation and differentiation of human dental pulp cells (HDPCs). The CSF scaffolds were designed with 3D mapping software Solidworks. Three different aperture-sized scaffolds (CSF1–CSF3) were prepared by low-temperature deposition 3D printing technology. The morphology was observed by scanning electron microscope (SEM) and optical coherence tomography. The porosity, hydrophilicity and mechanical capacity of the scaffold were detected, respectively. HDPCs (third passage, 1 × 105 cells) were seeded into each scaffold and investigated by SEM, CCK-8, alkaline phosphatase (ALP) activity and HE staining. The CSF scaffolds had porous structures with macropores and micropores. The macropore size of CSF1 to CSF3 was 421 ± 27 μm, 579 ± 36 μm and 707 ± 43 μm, respectively. The porosity was 69.8 ± 2.2%, 80.1 ± 2.8% and 86.5 ± 3.3%, respectively. All these scaffolds enhanced the adhesion and proliferation of HDPCs. The ALP activity in the CSF1 group was higher than that in the CSF3 groups (P < 0.01). HE staining showed HDPCs grew in multilayer within the scaffolds. CSF scaffolds significantly improved the adhesion and ALP activity of HDPCs. CSF scaffolds were promising candidates in dentine-pulp complex regeneration.</description><identifier>ISSN: 2056-3418</identifier><identifier>EISSN: 2056-3426</identifier><identifier>DOI: 10.1093/rb/rbab028</identifier><identifier>PMID: 34188954</identifier><language>eng</language><publisher>Oxford University Press</publisher><subject>3D printing ; Collagen ; Mapping software ; Phosphatases ; Porosity ; Silk ; Tissue engineering</subject><ispartof>Regenerative Biomaterials, 2021-08, Vol.8 (4), p.1</ispartof><rights>The Author(s) 2021. Published by Oxford University Press. 2021</rights><rights>COPYRIGHT 2021 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-89d0af67a3a97c92ee0a17fcf774927bd4fd7e2a2fef7fbe72e4f6639474c4173</citedby><cites>FETCH-LOGICAL-c518t-89d0af67a3a97c92ee0a17fcf774927bd4fd7e2a2fef7fbe72e4f6639474c4173</cites><orcidid>0000-0001-7931-5264</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226109/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226109/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1598,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Jiang, Shihui</creatorcontrib><creatorcontrib>Yu, Zhaoxia</creatorcontrib><creatorcontrib>Zhang, Lanrui</creatorcontrib><creatorcontrib>Wang, Guanhua</creatorcontrib><creatorcontrib>Dai, Xiaohua</creatorcontrib><creatorcontrib>Lian, Xiaoli</creatorcontrib><creatorcontrib>Yan, Yan</creatorcontrib><creatorcontrib>Zhang, Linpu</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Li, Ruixin</creatorcontrib><creatorcontrib>Zou, Huiru</creatorcontrib><title>Effects of different aperture-sized type I collagen/silk fibroin scaffolds on the proliferation and differentiation of human dental pulp cells</title><title>Regenerative Biomaterials</title><description>Abstract
This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on the proliferation and differentiation of human dental pulp cells (HDPCs). The CSF scaffolds were designed with 3D mapping software Solidworks. Three different aperture-sized scaffolds (CSF1–CSF3) were prepared by low-temperature deposition 3D printing technology. The morphology was observed by scanning electron microscope (SEM) and optical coherence tomography. The porosity, hydrophilicity and mechanical capacity of the scaffold were detected, respectively. HDPCs (third passage, 1 × 105 cells) were seeded into each scaffold and investigated by SEM, CCK-8, alkaline phosphatase (ALP) activity and HE staining. The CSF scaffolds had porous structures with macropores and micropores. The macropore size of CSF1 to CSF3 was 421 ± 27 μm, 579 ± 36 μm and 707 ± 43 μm, respectively. The porosity was 69.8 ± 2.2%, 80.1 ± 2.8% and 86.5 ± 3.3%, respectively. All these scaffolds enhanced the adhesion and proliferation of HDPCs. The ALP activity in the CSF1 group was higher than that in the CSF3 groups (P < 0.01). HE staining showed HDPCs grew in multilayer within the scaffolds. CSF scaffolds significantly improved the adhesion and ALP activity of HDPCs. CSF scaffolds were promising candidates in dentine-pulp complex regeneration.</description><subject>3D printing</subject><subject>Collagen</subject><subject>Mapping software</subject><subject>Phosphatases</subject><subject>Porosity</subject><subject>Silk</subject><subject>Tissue engineering</subject><issn>2056-3418</issn><issn>2056-3426</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>TOX</sourceid><recordid>eNp9kcFq3DAQhkVpacI2lz6BLr0UnEiyLNmXQghpGwjk0p6FLM3sqtVaRrIL6UP0maPFIaWXogENv_75ZhgR8p6zS86G9iqPNezIRP-KnAvWqaaVQr1-yXl_Ri5K-cEY41Irxbu35Owk90Mnz8mfW0RwS6EJqQ81zzAt1M6QlzVDU8Jv8HR5nIHeUZditHuYrkqIPymGMacw0eIsYoq-Iia6HIDOOcVQQXYJVbGT_wsOm1Z7Hdajnaivmo10XuNMHcRY3pE3aGOBi-d7R75_vv1287W5f_hyd3N937iO90vTD55ZVNq2dtBuEADMco0OtZaD0KOX6DUIKxBQ4whagESl2kFq6STX7Y582rjzOh7BuzpHttHMORxtfjTJBvPvyxQOZp9-mV4Iddr7jlxugL2NYMKEqdpcPR6OwaUJMFT9WnPRdYpLVgs-bgUup1Iy4EszzsyJaPJonn-ymj9s5rTO__M9AQxEodk</recordid><startdate>20210801</startdate><enddate>20210801</enddate><creator>Jiang, Shihui</creator><creator>Yu, Zhaoxia</creator><creator>Zhang, Lanrui</creator><creator>Wang, Guanhua</creator><creator>Dai, Xiaohua</creator><creator>Lian, Xiaoli</creator><creator>Yan, Yan</creator><creator>Zhang, Linpu</creator><creator>Wang, Yue</creator><creator>Li, Ruixin</creator><creator>Zou, Huiru</creator><general>Oxford University Press</general><scope>TOX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7931-5264</orcidid></search><sort><creationdate>20210801</creationdate><title>Effects of different aperture-sized type I collagen/silk fibroin scaffolds on the proliferation and differentiation of human dental pulp cells</title><author>Jiang, Shihui ; Yu, Zhaoxia ; Zhang, Lanrui ; Wang, Guanhua ; Dai, Xiaohua ; Lian, Xiaoli ; Yan, Yan ; Zhang, Linpu ; Wang, Yue ; Li, Ruixin ; Zou, Huiru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-89d0af67a3a97c92ee0a17fcf774927bd4fd7e2a2fef7fbe72e4f6639474c4173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>3D printing</topic><topic>Collagen</topic><topic>Mapping software</topic><topic>Phosphatases</topic><topic>Porosity</topic><topic>Silk</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Shihui</creatorcontrib><creatorcontrib>Yu, Zhaoxia</creatorcontrib><creatorcontrib>Zhang, Lanrui</creatorcontrib><creatorcontrib>Wang, Guanhua</creatorcontrib><creatorcontrib>Dai, Xiaohua</creatorcontrib><creatorcontrib>Lian, Xiaoli</creatorcontrib><creatorcontrib>Yan, Yan</creatorcontrib><creatorcontrib>Zhang, Linpu</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Li, Ruixin</creatorcontrib><creatorcontrib>Zou, Huiru</creatorcontrib><collection>Oxford Journals Open Access Collection</collection><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Regenerative Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Shihui</au><au>Yu, Zhaoxia</au><au>Zhang, Lanrui</au><au>Wang, Guanhua</au><au>Dai, Xiaohua</au><au>Lian, Xiaoli</au><au>Yan, Yan</au><au>Zhang, Linpu</au><au>Wang, Yue</au><au>Li, Ruixin</au><au>Zou, Huiru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of different aperture-sized type I collagen/silk fibroin scaffolds on the proliferation and differentiation of human dental pulp cells</atitle><jtitle>Regenerative Biomaterials</jtitle><date>2021-08-01</date><risdate>2021</risdate><volume>8</volume><issue>4</issue><spage>1</spage><pages>1-</pages><issn>2056-3418</issn><eissn>2056-3426</eissn><abstract>Abstract
This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on the proliferation and differentiation of human dental pulp cells (HDPCs). The CSF scaffolds were designed with 3D mapping software Solidworks. Three different aperture-sized scaffolds (CSF1–CSF3) were prepared by low-temperature deposition 3D printing technology. The morphology was observed by scanning electron microscope (SEM) and optical coherence tomography. The porosity, hydrophilicity and mechanical capacity of the scaffold were detected, respectively. HDPCs (third passage, 1 × 105 cells) were seeded into each scaffold and investigated by SEM, CCK-8, alkaline phosphatase (ALP) activity and HE staining. The CSF scaffolds had porous structures with macropores and micropores. The macropore size of CSF1 to CSF3 was 421 ± 27 μm, 579 ± 36 μm and 707 ± 43 μm, respectively. The porosity was 69.8 ± 2.2%, 80.1 ± 2.8% and 86.5 ± 3.3%, respectively. All these scaffolds enhanced the adhesion and proliferation of HDPCs. The ALP activity in the CSF1 group was higher than that in the CSF3 groups (P < 0.01). HE staining showed HDPCs grew in multilayer within the scaffolds. CSF scaffolds significantly improved the adhesion and ALP activity of HDPCs. CSF scaffolds were promising candidates in dentine-pulp complex regeneration.</abstract><pub>Oxford University Press</pub><pmid>34188954</pmid><doi>10.1093/rb/rbab028</doi><orcidid>https://orcid.org/0000-0001-7931-5264</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2056-3418 |
| ispartof | Regenerative Biomaterials, 2021-08, Vol.8 (4), p.1 |
| issn | 2056-3418 2056-3426 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8226109 |
| source | Oxford Journals Open Access Collection; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | 3D printing Collagen Mapping software Phosphatases Porosity Silk Tissue engineering |
| title | Effects of different aperture-sized type I collagen/silk fibroin scaffolds on the proliferation and differentiation of human dental pulp cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T17%3A15%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20different%20aperture-sized%20type%20I%20collagen/silk%20fibroin%20scaffolds%20on%20the%20proliferation%20and%20differentiation%20of%20human%20dental%20pulp%20cells&rft.jtitle=Regenerative%20Biomaterials&rft.au=Jiang,%20Shihui&rft.date=2021-08-01&rft.volume=8&rft.issue=4&rft.spage=1&rft.pages=1-&rft.issn=2056-3418&rft.eissn=2056-3426&rft_id=info:doi/10.1093/rb/rbab028&rft_dat=%3Cgale_pubme%3EA712556140%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/34188954&rft_galeid=A712556140&rft_oup_id=10.1093/rb/rbab028&rfr_iscdi=true |