new indicator cell line established to monitor bovine foamy virus infection
In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro, we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter (LTR, from −7 to 1012). The B...
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| Veröffentlicht in: | Virologica Sinica 2011-10, Vol.26 (5), p.315-323 |
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| creator | Guo, Hong-yan Liang, Zhi-bin Li, Yue Tan, Juan Chen, Qi-min Qiao, Wen-tao |
| description | In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro, we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter (LTR, from −7 to 1012). The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR. One clone, designated BFVL, was selected from ten neomycin-resistant clones. BFVL showed a specific and inducible dose- and time-dependent luciferase activity in response to BFV infection. Although the changes in luciferase activity of BFVL peaked at 84 h post infection, it was possible to differentiate infected and uninfected cells at 48 h post infection. A linear relationship was established between the multiplicity of infection (MOI) of BFV and the activated ratio of luciferase expression in BFVL. Moreover, the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection. These findings suggest that the BFVL-based assay is rapid, easy, sensitive, quantitative and specific for detection of BFV infection. |
| doi_str_mv | 10.1007/s12250-011-3204-y |
| format | Article |
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The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR. One clone, designated BFVL, was selected from ten neomycin-resistant clones. BFVL showed a specific and inducible dose- and time-dependent luciferase activity in response to BFV infection. Although the changes in luciferase activity of BFVL peaked at 84 h post infection, it was possible to differentiate infected and uninfected cells at 48 h post infection. A linear relationship was established between the multiplicity of infection (MOI) of BFV and the activated ratio of luciferase expression in BFVL. Moreover, the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection. These findings suggest that the BFVL-based assay is rapid, easy, sensitive, quantitative and specific for detection of BFV infection.</description><identifier>ISSN: 1674-0769</identifier><identifier>EISSN: 1995-820X</identifier><identifier>DOI: 10.1007/s12250-011-3204-y</identifier><identifier>PMID: 21979571</identifier><language>eng</language><publisher>Heidelberg: Springer-Verlag</publisher><subject>Animals ; Artificial Gene Fusion ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bovine foamy virus ; Cattle ; Cattle Diseases - virology ; Cell Line ; clones ; Cricetinae ; Fluorescence ; Foamy virus ; Genes, Reporter ; hamsters ; kidneys ; luciferase ; Luciferases - genetics ; Luciferases - metabolism ; Medical Microbiology ; Microbial Genetics and Genomics ; Microbiology ; Oncology ; Plasmids ; Retroviridae Infections - diagnosis ; Retroviridae Infections - veterinary ; Retroviridae Infections - virology ; Sensitivity and Specificity ; Spumavirus - isolation & purification ; terminal repeat sequences ; Viral Load - methods ; Virology</subject><ispartof>Virologica Sinica, 2011-10, Vol.26 (5), p.315-323</ispartof><rights>Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2011</rights><rights>Copyright © Wanfang Data Co. 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All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4434-5e6c88dc4e230c82f7ffa83dcd3fc5685a92d30203c68149efe492fdecdd4e0b3</citedby><cites>FETCH-LOGICAL-c4434-5e6c88dc4e230c82f7ffa83dcd3fc5685a92d30203c68149efe492fdecdd4e0b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zgbdx/zgbdx.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222431/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222431/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21979571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Hong-yan</creatorcontrib><creatorcontrib>Liang, Zhi-bin</creatorcontrib><creatorcontrib>Li, Yue</creatorcontrib><creatorcontrib>Tan, Juan</creatorcontrib><creatorcontrib>Chen, Qi-min</creatorcontrib><creatorcontrib>Qiao, Wen-tao</creatorcontrib><title>new indicator cell line established to monitor bovine foamy virus infection</title><title>Virologica Sinica</title><addtitle>Virol. Sin</addtitle><addtitle>Virol Sin</addtitle><description>In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro, we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter (LTR, from −7 to 1012). The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR. One clone, designated BFVL, was selected from ten neomycin-resistant clones. BFVL showed a specific and inducible dose- and time-dependent luciferase activity in response to BFV infection. Although the changes in luciferase activity of BFVL peaked at 84 h post infection, it was possible to differentiate infected and uninfected cells at 48 h post infection. A linear relationship was established between the multiplicity of infection (MOI) of BFV and the activated ratio of luciferase expression in BFVL. Moreover, the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection. These findings suggest that the BFVL-based assay is rapid, easy, sensitive, quantitative and specific for detection of BFV infection.</description><subject>Animals</subject><subject>Artificial Gene Fusion</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bovine foamy virus</subject><subject>Cattle</subject><subject>Cattle Diseases - virology</subject><subject>Cell Line</subject><subject>clones</subject><subject>Cricetinae</subject><subject>Fluorescence</subject><subject>Foamy virus</subject><subject>Genes, Reporter</subject><subject>hamsters</subject><subject>kidneys</subject><subject>luciferase</subject><subject>Luciferases - genetics</subject><subject>Luciferases - metabolism</subject><subject>Medical Microbiology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Oncology</subject><subject>Plasmids</subject><subject>Retroviridae Infections - diagnosis</subject><subject>Retroviridae Infections - veterinary</subject><subject>Retroviridae Infections - virology</subject><subject>Sensitivity and Specificity</subject><subject>Spumavirus - isolation & purification</subject><subject>terminal repeat sequences</subject><subject>Viral Load - methods</subject><subject>Virology</subject><issn>1674-0769</issn><issn>1995-820X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkstu1jAQhS0EoqXwAGwgK1gFxpck9gYJVVwqKnUBldhZji-pq8QudvKXv0-Po5RCN7CypfnO8cwcI_QcwxsM0L3NmJAGasC4pgRYvX-ADrEQTc0JfH9Y7m3HauhacYCe5HwJ0BJO6WN0QLDoRNPhQ_Ql2OvKB-O1mmOqtB3HavTBVjbPqh99vrCmmmM1xeBXoI-7teqimvbVzqclF7WzevYxPEWPnBqzfXZ7HqHzjx--HX-uT88-nRy_P601Y5TVjW0150YzSyhoTlznnOLUaEOdblreKEEMBQJUtxwzYZ1lgjhjtTHMQk-P0LvN92rpJ2u0DXNSo7xKflJpL6Py8n4l-As5xJ3khBBGcTF4tRlcq-BUGORlXFIoLcuboTc_SVkoNACsgK9vX0rxx1JWIief1x2pYOOSpQDGOODm_yQXLScd7aCQeCN1ijkn6-4axyDXWOUWqyxdyDVWuS-aF39PfKf4nWMByAbkUgqDTX9G-pfry03kVJRqSD7L869leAblp7RYcPoLLhu4pg</recordid><startdate>201110</startdate><enddate>201110</enddate><creator>Guo, Hong-yan</creator><creator>Liang, Zhi-bin</creator><creator>Li, Yue</creator><creator>Tan, Juan</creator><creator>Chen, Qi-min</creator><creator>Qiao, Wen-tao</creator><general>Springer-Verlag</general><general>SP Wuhan Institute of Virology, CAS</general><general>Key Laboratory of Molecular Microbiology and Biotechnology,Ministry of Education,and Key Laboratory of Microbial Functional Genomics of Tianjin,College of Life Sciences,Nankai University,Tianjin 300071,China</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>201110</creationdate><title>new indicator cell line established to monitor bovine foamy virus infection</title><author>Guo, Hong-yan ; Liang, Zhi-bin ; Li, Yue ; Tan, Juan ; Chen, Qi-min ; Qiao, Wen-tao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4434-5e6c88dc4e230c82f7ffa83dcd3fc5685a92d30203c68149efe492fdecdd4e0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Artificial Gene Fusion</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bovine foamy virus</topic><topic>Cattle</topic><topic>Cattle Diseases - virology</topic><topic>Cell Line</topic><topic>clones</topic><topic>Cricetinae</topic><topic>Fluorescence</topic><topic>Foamy virus</topic><topic>Genes, Reporter</topic><topic>hamsters</topic><topic>kidneys</topic><topic>luciferase</topic><topic>Luciferases - genetics</topic><topic>Luciferases - metabolism</topic><topic>Medical Microbiology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Oncology</topic><topic>Plasmids</topic><topic>Retroviridae Infections - diagnosis</topic><topic>Retroviridae Infections - veterinary</topic><topic>Retroviridae Infections - virology</topic><topic>Sensitivity and Specificity</topic><topic>Spumavirus - isolation & purification</topic><topic>terminal repeat sequences</topic><topic>Viral Load - methods</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Hong-yan</creatorcontrib><creatorcontrib>Liang, Zhi-bin</creatorcontrib><creatorcontrib>Li, Yue</creatorcontrib><creatorcontrib>Tan, Juan</creatorcontrib><creatorcontrib>Chen, Qi-min</creatorcontrib><creatorcontrib>Qiao, Wen-tao</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Hong-yan</au><au>Liang, Zhi-bin</au><au>Li, Yue</au><au>Tan, Juan</au><au>Chen, Qi-min</au><au>Qiao, Wen-tao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>new indicator cell line established to monitor bovine foamy virus infection</atitle><jtitle>Virologica Sinica</jtitle><stitle>Virol. Sin</stitle><addtitle>Virol Sin</addtitle><date>2011-10</date><risdate>2011</risdate><volume>26</volume><issue>5</issue><spage>315</spage><epage>323</epage><pages>315-323</pages><issn>1674-0769</issn><eissn>1995-820X</eissn><abstract>In order to improve the accuracy for quantitating the bovine foamy virus (BFV) in vitro, we developed a baby hamster kidney cell (BHK)-21-derived indicator cell line containing a plasmid that encodes the firefly luciferase driven by the BFV long terminal repeat promoter (LTR, from −7 to 1012). The BFV titer could be determined by detecting the luciferase expression since the viral trans-activator BTas protein activates the promoter activity of the LTR. One clone, designated BFVL, was selected from ten neomycin-resistant clones. BFVL showed a specific and inducible dose- and time-dependent luciferase activity in response to BFV infection. Although the changes in luciferase activity of BFVL peaked at 84 h post infection, it was possible to differentiate infected and uninfected cells at 48 h post infection. A linear relationship was established between the multiplicity of infection (MOI) of BFV and the activated ratio of luciferase expression in BFVL. Moreover, the sensitivity of the BFVL-based assay for detecting infectious BFV was 10,000 times higher than the conventional CPE-based assay at 48 h post infection. These findings suggest that the BFVL-based assay is rapid, easy, sensitive, quantitative and specific for detection of BFV infection.</abstract><cop>Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21979571</pmid><doi>10.1007/s12250-011-3204-y</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Virologica Sinica, 2011-10, Vol.26 (5), p.315-323 |
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| language | eng |
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| source | MEDLINE; SpringerNature Complete Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Artificial Gene Fusion Biochemistry Biomedical and Life Sciences Biomedicine Bovine foamy virus Cattle Cattle Diseases - virology Cell Line clones Cricetinae Fluorescence Foamy virus Genes, Reporter hamsters kidneys luciferase Luciferases - genetics Luciferases - metabolism Medical Microbiology Microbial Genetics and Genomics Microbiology Oncology Plasmids Retroviridae Infections - diagnosis Retroviridae Infections - veterinary Retroviridae Infections - virology Sensitivity and Specificity Spumavirus - isolation & purification terminal repeat sequences Viral Load - methods Virology |
| title | new indicator cell line established to monitor bovine foamy virus infection |
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