rapid and sensitive one step-SYBR green based semi quantitative real time RT-PCR for the detection of peste des petits ruminants virus in the clinical samples

A sensitive and rapid single step real time (rt) RT-PCR was standardized using one-step Brilliant SYBR Green kit® for detection and semi-quantitation of peste des petitis ruminants virus (PPRV) using the virus RNA and matrix (M) protein gene-specific primers and compared with established conventiona...

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Veröffentlicht in:	Virologica Sinica 2012-02, Vol.27 (1), p.1-9
Hauptverfasser:	Balamurugan, Vinayagamurthy, Sen, Arnab, Venkatesan, Gnanavel, Yadav, Vinita, Bhanot, Vandna, Bhanuprakash, Veerakyathappa, Singh, Raj Kumar
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biochemistry Biomedical and Life Sciences Biomedicine Cell culture Contamination detection limit DNA Primers - genetics genes Goats M gene Medical Microbiology Microbial Genetics and Genomics Microbiology Molecular Diagnostic Techniques - methods Molecular Diagnostic Techniques - standards Oncology Organic Chemicals - metabolism Peste des petits ruminants Peste-des-Petits-Ruminants - diagnosis Peste-des-petits-ruminants virus - genetics Peste-des-petits-ruminants virus - isolation & purification Polymerase chain reaction Quantitation Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Ribonucleic acid risk RNA RNA viruses RNA, Viral - genetics Ruminantia Sensitivity and Specificity Sheep Staining and Labeling - methods Time Factors Vaccines Veterinary Medicine - methods Veterinary Medicine - standards Viral Matrix Proteins - genetics Virology Virology - methods Virology - standards viruses
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