Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy
Astrocytomas are the most common and aggressive primary brain tumors, and they are classified according to the degree of malignancy on a scale of I to IV, in which grade I is the least malignant and grade IV the highest. Many factors are related to astrocytomas progression as progesterone receptor (...
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| Veröffentlicht in: | OncoTargets and therapy 2021-01, Vol.14, p.3757-3768 |
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| creator | Arcos-Montoya, Denisse Wegman-Ostrosky, Talia Mejía-Pérez, Sonia De la Fuente-Granada, Marisol Camacho-Arroyo, Ignacio García-Carrancá, Alejandro Velasco-Velázquez, Marco A Manjarrez-Marmolejo, Joaquín González-Arenas, Aliesha |
| description | Astrocytomas are the most common and aggressive primary brain tumors, and they are classified according to the degree of malignancy on a scale of I to IV, in which grade I is the least malignant and grade IV the highest. Many factors are related to astrocytomas progression as progesterone receptor (PR), whose transcriptional activity could be regulated by phosphorylation by protein kinase C alpha (PKCα) at the residue Ser400. Our aim was to investigate if PR phosphorylation together with PKCα expression could be used as a prognostic factor for astrocytomas malignancy.
By immunofluorescence, we detected the content of PKCα, PR and its phosphorylation at Ser400 in 46 biopsies from Mexican patients with different astrocytoma malignancy grades; by bioinformatic tools using TCGA data, we evaluated the expression of PR and PKCα mRNA according to astrocytoma malignancy grades. For all statistical analyses, significance was p |
| doi_str_mv | 10.2147/OTT.S280314 |
| format | Article |
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By immunofluorescence, we detected the content of PKCα, PR and its phosphorylation at Ser400 in 46 biopsies from Mexican patients with different astrocytoma malignancy grades; by bioinformatic tools using TCGA data, we evaluated the expression of PR and PKCα mRNA according to astrocytoma malignancy grades. For all statistical analyses, significance was p<0.05.
We detected a positive correlation between the tumor grade and the content of PKCα, PR and its phosphorylation at Ser400, as well as the intracellular colocalization of these proteins. Interestingly, using an in silico assay, we found that the PR and PKCα expression at mRNA level has an inverse ratio with astrocytomas tumor grade.
These results indicate that PR and its phosphorylation at Ser400 site, as well as PKCα and their colocalization, could be considered as possible malignancy biomarkers for astrocytomas grades I-IV.</description><identifier>ISSN: 1178-6930</identifier><identifier>EISSN: 1178-6930</identifier><identifier>DOI: 10.2147/OTT.S280314</identifier><identifier>PMID: 34168461</identifier><language>eng</language><publisher>New Zealand: Taylor & Francis Ltd</publisher><subject>Antibodies ; Astrocytoma ; Biopsy ; Brain cancer ; Brain tumors ; Ethanol ; Gene expression ; Immunofluorescence ; Kinases ; Ligands ; Localization ; Malignancy ; Medical prognosis ; Neurosurgery ; Original Research ; Pathology ; Phosphorylation ; Progesterone ; Protein kinase C ; Statistical analysis ; Transcription ; Tumors</subject><ispartof>OncoTargets and therapy, 2021-01, Vol.14, p.3757-3768</ispartof><rights>2021 Arcos-Montoya et al.</rights><rights>2021. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Arcos-Montoya et al. 2021 Arcos-Montoya et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-498790fd9849c23137c7f955ee29e738c9237c070d0da2a23dc14588a02e522b3</citedby><cites>FETCH-LOGICAL-c409t-498790fd9849c23137c7f955ee29e738c9237c070d0da2a23dc14588a02e522b3</cites><orcidid>0000-0001-9717-0265 ; 0000-0002-6509-7662 ; 0000-0002-5929-6761 ; 0000-0003-1220-5014 ; 0000-0002-9506-3180</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217595/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217595/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,3849,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34168461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arcos-Montoya, Denisse</creatorcontrib><creatorcontrib>Wegman-Ostrosky, Talia</creatorcontrib><creatorcontrib>Mejía-Pérez, Sonia</creatorcontrib><creatorcontrib>De la Fuente-Granada, Marisol</creatorcontrib><creatorcontrib>Camacho-Arroyo, Ignacio</creatorcontrib><creatorcontrib>García-Carrancá, Alejandro</creatorcontrib><creatorcontrib>Velasco-Velázquez, Marco A</creatorcontrib><creatorcontrib>Manjarrez-Marmolejo, Joaquín</creatorcontrib><creatorcontrib>González-Arenas, Aliesha</creatorcontrib><title>Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy</title><title>OncoTargets and therapy</title><addtitle>Onco Targets Ther</addtitle><description>Astrocytomas are the most common and aggressive primary brain tumors, and they are classified according to the degree of malignancy on a scale of I to IV, in which grade I is the least malignant and grade IV the highest. Many factors are related to astrocytomas progression as progesterone receptor (PR), whose transcriptional activity could be regulated by phosphorylation by protein kinase C alpha (PKCα) at the residue Ser400. Our aim was to investigate if PR phosphorylation together with PKCα expression could be used as a prognostic factor for astrocytomas malignancy.
By immunofluorescence, we detected the content of PKCα, PR and its phosphorylation at Ser400 in 46 biopsies from Mexican patients with different astrocytoma malignancy grades; by bioinformatic tools using TCGA data, we evaluated the expression of PR and PKCα mRNA according to astrocytoma malignancy grades. For all statistical analyses, significance was p<0.05.
We detected a positive correlation between the tumor grade and the content of PKCα, PR and its phosphorylation at Ser400, as well as the intracellular colocalization of these proteins. Interestingly, using an in silico assay, we found that the PR and PKCα expression at mRNA level has an inverse ratio with astrocytomas tumor grade.
These results indicate that PR and its phosphorylation at Ser400 site, as well as PKCα and their colocalization, could be considered as possible malignancy biomarkers for astrocytomas grades I-IV.</description><subject>Antibodies</subject><subject>Astrocytoma</subject><subject>Biopsy</subject><subject>Brain cancer</subject><subject>Brain tumors</subject><subject>Ethanol</subject><subject>Gene expression</subject><subject>Immunofluorescence</subject><subject>Kinases</subject><subject>Ligands</subject><subject>Localization</subject><subject>Malignancy</subject><subject>Medical prognosis</subject><subject>Neurosurgery</subject><subject>Original Research</subject><subject>Pathology</subject><subject>Phosphorylation</subject><subject>Progesterone</subject><subject>Protein kinase C</subject><subject>Statistical analysis</subject><subject>Transcription</subject><subject>Tumors</subject><issn>1178-6930</issn><issn>1178-6930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc1OWzEQha2qqNDAqvvKUjdIKKl_77U3laKIn6pUIAhry_jOTS66sYPttOSx-iI8E44IiLKyNfOdo5k5CH2hZMSoqL9fTKeja6YIp-ID2qO0VsNKc_LxzX8XfU7pjpCqUkx8Qrtc0EqJiu6h9jKGGaQMMXjAV-BgmUPE01LMc4j4b5fn-PLX5PEfPn5YRkipCx7bhDc6H1LuHD6xrmgSbotwnHIMbp3DojC_bd_NvPVuvY92WtsnONi-A3RzcjydnA3PL05_TsbnQyeIzkOhVa1J22gltGOc8trVrZYSgGmouXKalRKpSUMayyzjjaNCKmUJA8nYLR-gH8--y9XtAhoHPkfbm2XsFjauTbCd-b_ju7mZhT9GMVpLLYvB4dYghvtVOYxZdMlB31sPYZUMk0JKzSWrCvrtHXoXVtGX9TYUVVUlNC_U0TPlYkgpQvs6DCVmk58p-ZltfoX--nb-V_YlMP4EZW2XnQ</recordid><startdate>20210101</startdate><enddate>20210101</enddate><creator>Arcos-Montoya, Denisse</creator><creator>Wegman-Ostrosky, Talia</creator><creator>Mejía-Pérez, Sonia</creator><creator>De la Fuente-Granada, Marisol</creator><creator>Camacho-Arroyo, Ignacio</creator><creator>García-Carrancá, Alejandro</creator><creator>Velasco-Velázquez, Marco A</creator><creator>Manjarrez-Marmolejo, Joaquín</creator><creator>González-Arenas, Aliesha</creator><general>Taylor & Francis Ltd</general><general>Dove</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9717-0265</orcidid><orcidid>https://orcid.org/0000-0002-6509-7662</orcidid><orcidid>https://orcid.org/0000-0002-5929-6761</orcidid><orcidid>https://orcid.org/0000-0003-1220-5014</orcidid><orcidid>https://orcid.org/0000-0002-9506-3180</orcidid></search><sort><creationdate>20210101</creationdate><title>Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy</title><author>Arcos-Montoya, Denisse ; Wegman-Ostrosky, Talia ; Mejía-Pérez, Sonia ; De la Fuente-Granada, Marisol ; Camacho-Arroyo, Ignacio ; García-Carrancá, Alejandro ; Velasco-Velázquez, Marco A ; Manjarrez-Marmolejo, Joaquín ; González-Arenas, Aliesha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-498790fd9849c23137c7f955ee29e738c9237c070d0da2a23dc14588a02e522b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibodies</topic><topic>Astrocytoma</topic><topic>Biopsy</topic><topic>Brain cancer</topic><topic>Brain tumors</topic><topic>Ethanol</topic><topic>Gene expression</topic><topic>Immunofluorescence</topic><topic>Kinases</topic><topic>Ligands</topic><topic>Localization</topic><topic>Malignancy</topic><topic>Medical prognosis</topic><topic>Neurosurgery</topic><topic>Original Research</topic><topic>Pathology</topic><topic>Phosphorylation</topic><topic>Progesterone</topic><topic>Protein kinase C</topic><topic>Statistical analysis</topic><topic>Transcription</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arcos-Montoya, Denisse</creatorcontrib><creatorcontrib>Wegman-Ostrosky, Talia</creatorcontrib><creatorcontrib>Mejía-Pérez, Sonia</creatorcontrib><creatorcontrib>De la Fuente-Granada, Marisol</creatorcontrib><creatorcontrib>Camacho-Arroyo, Ignacio</creatorcontrib><creatorcontrib>García-Carrancá, Alejandro</creatorcontrib><creatorcontrib>Velasco-Velázquez, Marco A</creatorcontrib><creatorcontrib>Manjarrez-Marmolejo, Joaquín</creatorcontrib><creatorcontrib>González-Arenas, Aliesha</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>OncoTargets and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arcos-Montoya, Denisse</au><au>Wegman-Ostrosky, Talia</au><au>Mejía-Pérez, Sonia</au><au>De la Fuente-Granada, Marisol</au><au>Camacho-Arroyo, Ignacio</au><au>García-Carrancá, Alejandro</au><au>Velasco-Velázquez, Marco A</au><au>Manjarrez-Marmolejo, Joaquín</au><au>González-Arenas, Aliesha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy</atitle><jtitle>OncoTargets and therapy</jtitle><addtitle>Onco Targets Ther</addtitle><date>2021-01-01</date><risdate>2021</risdate><volume>14</volume><spage>3757</spage><epage>3768</epage><pages>3757-3768</pages><issn>1178-6930</issn><eissn>1178-6930</eissn><abstract>Astrocytomas are the most common and aggressive primary brain tumors, and they are classified according to the degree of malignancy on a scale of I to IV, in which grade I is the least malignant and grade IV the highest. Many factors are related to astrocytomas progression as progesterone receptor (PR), whose transcriptional activity could be regulated by phosphorylation by protein kinase C alpha (PKCα) at the residue Ser400. Our aim was to investigate if PR phosphorylation together with PKCα expression could be used as a prognostic factor for astrocytomas malignancy.
By immunofluorescence, we detected the content of PKCα, PR and its phosphorylation at Ser400 in 46 biopsies from Mexican patients with different astrocytoma malignancy grades; by bioinformatic tools using TCGA data, we evaluated the expression of PR and PKCα mRNA according to astrocytoma malignancy grades. For all statistical analyses, significance was p<0.05.
We detected a positive correlation between the tumor grade and the content of PKCα, PR and its phosphorylation at Ser400, as well as the intracellular colocalization of these proteins. Interestingly, using an in silico assay, we found that the PR and PKCα expression at mRNA level has an inverse ratio with astrocytomas tumor grade.
These results indicate that PR and its phosphorylation at Ser400 site, as well as PKCα and their colocalization, could be considered as possible malignancy biomarkers for astrocytomas grades I-IV.</abstract><cop>New Zealand</cop><pub>Taylor & Francis Ltd</pub><pmid>34168461</pmid><doi>10.2147/OTT.S280314</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-9717-0265</orcidid><orcidid>https://orcid.org/0000-0002-6509-7662</orcidid><orcidid>https://orcid.org/0000-0002-5929-6761</orcidid><orcidid>https://orcid.org/0000-0003-1220-5014</orcidid><orcidid>https://orcid.org/0000-0002-9506-3180</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Astrocytoma Biopsy Brain cancer Brain tumors Ethanol Gene expression Immunofluorescence Kinases Ligands Localization Malignancy Medical prognosis Neurosurgery Original Research Pathology Phosphorylation Progesterone Protein kinase C Statistical analysis Transcription Tumors |
| title | Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy |
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