Synaptosomal Actin Dynamics in the Developmental Visual Cortex Regulate Behavioral Visual Acuity in Rats
Synaptosomal actin dynamics are essential for synaptic structural stability. Whether actin dynamics are involved in structural and functional synaptic plasticity within the primary visual cortex (V1) or behavioral visual acuity in rats has still not been thoroughly investigated. Synaptosome preparat...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2021-06, Vol.62 (7), p.20 |
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| creator | Bi, Ai-Ling Zhang, Yue-Ying Lu, Zhi-Yuan Tang, Hong-Ying Zhang, Xiu-Yan Zhang, Zi-Han Li, Bo-Qin Guo, Da-Dong Gong, Sheng Li, Qian Wang, Xing-Rong Lu, Xiu-Zhen Bi, Hong-Sheng |
| description | Synaptosomal actin dynamics are essential for synaptic structural stability. Whether actin dynamics are involved in structural and functional synaptic plasticity within the primary visual cortex (V1) or behavioral visual acuity in rats has still not been thoroughly investigated.
Synaptosome preparation and western blot analysis were used to analyze synaptosomal actin dynamics. Transmission electron microscopy was used to detect synaptic density and mitochondrial area alterations. A visual water maze task was applied to assess behavioral visual acuity. Microinjection of the actin polymerization inhibitor or stabilizer detected the effect of actin dynamics on visual function.
Actin dynamics, the mitochondrial area, and synaptic density within the area of V1 are increased during the critical period for the development of binocularity. Microinjection of the actin polymerization inhibitor cytochalasin D into the V1 decreased the mitochondrial area, synaptic density, and behavioral visual acuity. Long-term monocular deprivation reduced actin dynamics, the mitochondrial area, and synaptic density within the V1 contralateral to the deprived eye compared with those ipsilateral to the deprived eye and impaired visual acuity in the amblyopic eye. In addition, the mitochondrial area, synaptic density, and behavioral visual acuity were improved by stabilization of actin polymerization by jasplakinolide microinjection.
During the critical period of visual development of binocularity, synaptosomal actin dynamics regulate synaptic structure and function and play roles in behavioral visual acuity in rats. |
| doi_str_mv | 10.1167/iovs.62.7.20 |
| format | Article |
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Synaptosome preparation and western blot analysis were used to analyze synaptosomal actin dynamics. Transmission electron microscopy was used to detect synaptic density and mitochondrial area alterations. A visual water maze task was applied to assess behavioral visual acuity. Microinjection of the actin polymerization inhibitor or stabilizer detected the effect of actin dynamics on visual function.
Actin dynamics, the mitochondrial area, and synaptic density within the area of V1 are increased during the critical period for the development of binocularity. Microinjection of the actin polymerization inhibitor cytochalasin D into the V1 decreased the mitochondrial area, synaptic density, and behavioral visual acuity. Long-term monocular deprivation reduced actin dynamics, the mitochondrial area, and synaptic density within the V1 contralateral to the deprived eye compared with those ipsilateral to the deprived eye and impaired visual acuity in the amblyopic eye. In addition, the mitochondrial area, synaptic density, and behavioral visual acuity were improved by stabilization of actin polymerization by jasplakinolide microinjection.
During the critical period of visual development of binocularity, synaptosomal actin dynamics regulate synaptic structure and function and play roles in behavioral visual acuity in rats.</description><identifier>ISSN: 1552-5783</identifier><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.62.7.20</identifier><identifier>PMID: 34137807</identifier><language>eng</language><publisher>United States: The Association for Research in Vision and Ophthalmology</publisher><subject>Actins - chemistry ; Actins - metabolism ; Amblyopia - metabolism ; Amblyopia - physiopathology ; Animals ; Antineoplastic Agents - pharmacology ; Behavior, Animal - physiology ; Depsipeptides - pharmacology ; Maze Learning ; Neuronal Plasticity - physiology ; Polymerization - drug effects ; Rats ; Synaptosomes - metabolism ; Vision, Ocular - physiology ; Visual Acuity - physiology ; Visual Cortex - physiology ; Visual Neuroscience</subject><ispartof>Investigative ophthalmology & visual science, 2021-06, Vol.62 (7), p.20</ispartof><rights>Copyright 2021 The Authors 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-d0a90c85f36e90d2941ee58d3d339b20281c4dd204297ad224a5f66b55e0bdd33</citedby><cites>FETCH-LOGICAL-c384t-d0a90c85f36e90d2941ee58d3d339b20281c4dd204297ad224a5f66b55e0bdd33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212442/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212442/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34137807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bi, Ai-Ling</creatorcontrib><creatorcontrib>Zhang, Yue-Ying</creatorcontrib><creatorcontrib>Lu, Zhi-Yuan</creatorcontrib><creatorcontrib>Tang, Hong-Ying</creatorcontrib><creatorcontrib>Zhang, Xiu-Yan</creatorcontrib><creatorcontrib>Zhang, Zi-Han</creatorcontrib><creatorcontrib>Li, Bo-Qin</creatorcontrib><creatorcontrib>Guo, Da-Dong</creatorcontrib><creatorcontrib>Gong, Sheng</creatorcontrib><creatorcontrib>Li, Qian</creatorcontrib><creatorcontrib>Wang, Xing-Rong</creatorcontrib><creatorcontrib>Lu, Xiu-Zhen</creatorcontrib><creatorcontrib>Bi, Hong-Sheng</creatorcontrib><title>Synaptosomal Actin Dynamics in the Developmental Visual Cortex Regulate Behavioral Visual Acuity in Rats</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>Synaptosomal actin dynamics are essential for synaptic structural stability. Whether actin dynamics are involved in structural and functional synaptic plasticity within the primary visual cortex (V1) or behavioral visual acuity in rats has still not been thoroughly investigated.
Synaptosome preparation and western blot analysis were used to analyze synaptosomal actin dynamics. Transmission electron microscopy was used to detect synaptic density and mitochondrial area alterations. A visual water maze task was applied to assess behavioral visual acuity. Microinjection of the actin polymerization inhibitor or stabilizer detected the effect of actin dynamics on visual function.
Actin dynamics, the mitochondrial area, and synaptic density within the area of V1 are increased during the critical period for the development of binocularity. Microinjection of the actin polymerization inhibitor cytochalasin D into the V1 decreased the mitochondrial area, synaptic density, and behavioral visual acuity. Long-term monocular deprivation reduced actin dynamics, the mitochondrial area, and synaptic density within the V1 contralateral to the deprived eye compared with those ipsilateral to the deprived eye and impaired visual acuity in the amblyopic eye. In addition, the mitochondrial area, synaptic density, and behavioral visual acuity were improved by stabilization of actin polymerization by jasplakinolide microinjection.
During the critical period of visual development of binocularity, synaptosomal actin dynamics regulate synaptic structure and function and play roles in behavioral visual acuity in rats.</description><subject>Actins - chemistry</subject><subject>Actins - metabolism</subject><subject>Amblyopia - metabolism</subject><subject>Amblyopia - physiopathology</subject><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Behavior, Animal - physiology</subject><subject>Depsipeptides - pharmacology</subject><subject>Maze Learning</subject><subject>Neuronal Plasticity - physiology</subject><subject>Polymerization - drug effects</subject><subject>Rats</subject><subject>Synaptosomes - metabolism</subject><subject>Vision, Ocular - physiology</subject><subject>Visual Acuity - physiology</subject><subject>Visual Cortex - physiology</subject><subject>Visual Neuroscience</subject><issn>1552-5783</issn><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUlLBDEQhYMo7jfP0kcPzphUkl4uwjiuIAhu15BJapxId2fspAfn35vB_VSPqo9XDx4hB4wOGcuLE-cXYZjDsBgCXSPbTEoYyKLk63_0FtkJ4ZVSYAzoJtnigvGipMU2mT0sWz2PPvhG19nIRNdm52nVOBOypOMMs3NcYO3nDbYxMc8u9GmMfRfxPbvHl77WEbMznOmF890vMTK9i8uVyb2OYY9sTHUdcP9r7pKny4vH8fXg9u7qZjy6HRheijiwVFfUlHLKc6yohUowRFlabjmvJkChZEZYC1RAVWgLILSc5vlESqQTm6BdcvrpO-8nDVqTQqdMat65RndL5bVT_y-tm6kXv1AlMBACksHRl0Hn33oMUTUuGKxr3aLvgwKZoJxKkSf0-BM1nQ-hw-nPG0bVqhy1KkfloAoFNOGHf6P9wN9t8A9bZI1t</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Bi, Ai-Ling</creator><creator>Zhang, Yue-Ying</creator><creator>Lu, Zhi-Yuan</creator><creator>Tang, Hong-Ying</creator><creator>Zhang, Xiu-Yan</creator><creator>Zhang, Zi-Han</creator><creator>Li, Bo-Qin</creator><creator>Guo, Da-Dong</creator><creator>Gong, Sheng</creator><creator>Li, Qian</creator><creator>Wang, Xing-Rong</creator><creator>Lu, Xiu-Zhen</creator><creator>Bi, Hong-Sheng</creator><general>The Association for Research in Vision and Ophthalmology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210601</creationdate><title>Synaptosomal Actin Dynamics in the Developmental Visual Cortex Regulate Behavioral Visual Acuity in Rats</title><author>Bi, Ai-Ling ; Zhang, Yue-Ying ; Lu, Zhi-Yuan ; Tang, Hong-Ying ; Zhang, Xiu-Yan ; Zhang, Zi-Han ; Li, Bo-Qin ; Guo, Da-Dong ; Gong, Sheng ; Li, Qian ; Wang, Xing-Rong ; Lu, Xiu-Zhen ; Bi, Hong-Sheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-d0a90c85f36e90d2941ee58d3d339b20281c4dd204297ad224a5f66b55e0bdd33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Actins - chemistry</topic><topic>Actins - metabolism</topic><topic>Amblyopia - metabolism</topic><topic>Amblyopia - physiopathology</topic><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Behavior, Animal - physiology</topic><topic>Depsipeptides - pharmacology</topic><topic>Maze Learning</topic><topic>Neuronal Plasticity - physiology</topic><topic>Polymerization - drug effects</topic><topic>Rats</topic><topic>Synaptosomes - metabolism</topic><topic>Vision, Ocular - physiology</topic><topic>Visual Acuity - physiology</topic><topic>Visual Cortex - physiology</topic><topic>Visual Neuroscience</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bi, Ai-Ling</creatorcontrib><creatorcontrib>Zhang, Yue-Ying</creatorcontrib><creatorcontrib>Lu, Zhi-Yuan</creatorcontrib><creatorcontrib>Tang, Hong-Ying</creatorcontrib><creatorcontrib>Zhang, Xiu-Yan</creatorcontrib><creatorcontrib>Zhang, Zi-Han</creatorcontrib><creatorcontrib>Li, Bo-Qin</creatorcontrib><creatorcontrib>Guo, Da-Dong</creatorcontrib><creatorcontrib>Gong, Sheng</creatorcontrib><creatorcontrib>Li, Qian</creatorcontrib><creatorcontrib>Wang, Xing-Rong</creatorcontrib><creatorcontrib>Lu, Xiu-Zhen</creatorcontrib><creatorcontrib>Bi, Hong-Sheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bi, Ai-Ling</au><au>Zhang, Yue-Ying</au><au>Lu, Zhi-Yuan</au><au>Tang, Hong-Ying</au><au>Zhang, Xiu-Yan</au><au>Zhang, Zi-Han</au><au>Li, Bo-Qin</au><au>Guo, Da-Dong</au><au>Gong, Sheng</au><au>Li, Qian</au><au>Wang, Xing-Rong</au><au>Lu, Xiu-Zhen</au><au>Bi, Hong-Sheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synaptosomal Actin Dynamics in the Developmental Visual Cortex Regulate Behavioral Visual Acuity in Rats</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2021-06-01</date><risdate>2021</risdate><volume>62</volume><issue>7</issue><spage>20</spage><pages>20-</pages><issn>1552-5783</issn><issn>0146-0404</issn><eissn>1552-5783</eissn><abstract>Synaptosomal actin dynamics are essential for synaptic structural stability. Whether actin dynamics are involved in structural and functional synaptic plasticity within the primary visual cortex (V1) or behavioral visual acuity in rats has still not been thoroughly investigated.
Synaptosome preparation and western blot analysis were used to analyze synaptosomal actin dynamics. Transmission electron microscopy was used to detect synaptic density and mitochondrial area alterations. A visual water maze task was applied to assess behavioral visual acuity. Microinjection of the actin polymerization inhibitor or stabilizer detected the effect of actin dynamics on visual function.
Actin dynamics, the mitochondrial area, and synaptic density within the area of V1 are increased during the critical period for the development of binocularity. Microinjection of the actin polymerization inhibitor cytochalasin D into the V1 decreased the mitochondrial area, synaptic density, and behavioral visual acuity. Long-term monocular deprivation reduced actin dynamics, the mitochondrial area, and synaptic density within the V1 contralateral to the deprived eye compared with those ipsilateral to the deprived eye and impaired visual acuity in the amblyopic eye. In addition, the mitochondrial area, synaptic density, and behavioral visual acuity were improved by stabilization of actin polymerization by jasplakinolide microinjection.
During the critical period of visual development of binocularity, synaptosomal actin dynamics regulate synaptic structure and function and play roles in behavioral visual acuity in rats.</abstract><cop>United States</cop><pub>The Association for Research in Vision and Ophthalmology</pub><pmid>34137807</pmid><doi>10.1167/iovs.62.7.20</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Investigative ophthalmology & visual science, 2021-06, Vol.62 (7), p.20 |
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| subjects | Actins - chemistry Actins - metabolism Amblyopia - metabolism Amblyopia - physiopathology Animals Antineoplastic Agents - pharmacology Behavior, Animal - physiology Depsipeptides - pharmacology Maze Learning Neuronal Plasticity - physiology Polymerization - drug effects Rats Synaptosomes - metabolism Vision, Ocular - physiology Visual Acuity - physiology Visual Cortex - physiology Visual Neuroscience |
| title | Synaptosomal Actin Dynamics in the Developmental Visual Cortex Regulate Behavioral Visual Acuity in Rats |
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