Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase
We developed a Gaussia luciferase （Gluc） reporter replicon of West Nile virus （WNV） and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the...
Gespeichert in:
| Veröffentlicht in: | Virologica Sinica 2013-06, Vol.28 (3), p.161-166 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 166 |
|---|---|
| container_issue | 3 |
| container_start_page | 161 |
| container_title | Virologica Sinica |
| container_volume | 28 |
| creator | Shan, Chao Li, Xiaodan Deng, Chenglin Shang, Baodi Xu, Linlin Ye, Hanqing Yuan, Zhiming Zhang, Bo |
| description | We developed a Gaussia luciferase （Gluc） reporter replicon of West Nile virus （WNV） and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor （NITD008）, we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV. |
| doi_str_mv | 10.1007/s12250-013-3332-7 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8208408</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>46210374</cqvip_id><wanfj_id>zgbdx201303006</wanfj_id><sourcerecordid>zgbdx201303006</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4716-4497a33a13d1d1bff7e708337109922966367a071376582dbdabd83320fc560f3</originalsourceid><addsrcrecordid>eNqFUU1v1DAQtRCIloUfwAXCBXEJjD9iJ5dKaCkFaQUSpcDNchIn6yprp_amlP56ZpVlgQucYuW9mXkfhDym8JICqFeJMlZADpTnnHOWqzvkmFZVkZcMvt3Ft1QiByWrI_IgpUsAyUrO75MjxhVUUFTHpHljr-0Qxo3128z4NluuTTTN1kZ3a7Yu-Cx02VebttkHN9jsi4tTyj7ZcXANYqc3Y7QpOd9n57aJdmvb7MxM-Mdkq6lxnY0m2YfkXmeGZB_tvwty8fb08_Jdvvp49n75epU3QlGZC1Epw7mhvKUtrbtOWQWoV1GoKsYqKblUBhTlShYla-vW1C3iDLqmkNDxBTmZ945TvbFtg5aiGfQY3cbEHzoYp_9GvFvrPlxrjKsUeGpBns8LvhvfGd_ryzBFj5L1bV-3NwyDBo4pIvHF_lIMVxOmozcuNXYYjLdhSpoKisUIyfj_qWiqLKkAgVQ6U5sYUoq2O0inoHeF67lwjTr0rnCtcObJn54PE78aRgKbCQkh39v429S_tj6dhzoTtOmjS_riHO0LQPeSFzutz_Za18H3V7j5cBptU-BK8J8jgcnG</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1367881404</pqid></control><display><type>article</type><title>Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase</title><source>MEDLINE</source><source>SpringerNature Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Shan, Chao ; Li, Xiaodan ; Deng, Chenglin ; Shang, Baodi ; Xu, Linlin ; Ye, Hanqing ; Yuan, Zhiming ; Zhang, Bo</creator><creatorcontrib>Shan, Chao ; Li, Xiaodan ; Deng, Chenglin ; Shang, Baodi ; Xu, Linlin ; Ye, Hanqing ; Yuan, Zhiming ; Zhang, Bo</creatorcontrib><description>We developed a Gaussia luciferase （Gluc） reporter replicon of West Nile virus （WNV） and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor （NITD008）, we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.</description><identifier>ISSN: 1674-0769</identifier><identifier>EISSN: 1995-820X</identifier><identifier>DOI: 10.1007/s12250-013-3332-7</identifier><identifier>PMID: 23709059</identifier><language>eng</language><publisher>Heidelberg: Springer-Verlag</publisher><subject>Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cell Line ; Cricetinae ; culture media ; Culture Media - chemistry ; Drug Evaluation, Preclinical - methods ; drugs ; Flavivirus ; Gaussia ; Genes, Reporter ; luciferase ; Luciferases - genetics ; Luciferases - secretion ; Medical Microbiology ; Microbial Genetics and Genomics ; Microbiology ; Oncology ; pathogenesis ; replicon ; Research Article ; RNA ; screening ; Staining and Labeling - methods ; translation (genetics) ; Virology ; virus replication ; West Nile virus ; West Nile virus - enzymology ; West Nile virus - genetics ; 复制子 ; 病毒复制 ; 病毒抑制剂 ; 细胞分泌 ; 荧光素酶 ; 药物开发 ; 表征 ; 西尼罗河病毒</subject><ispartof>Virologica Sinica, 2013-06, Vol.28 (3), p.161-166</ispartof><rights>Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2013</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4716-4497a33a13d1d1bff7e708337109922966367a071376582dbdabd83320fc560f3</citedby><cites>FETCH-LOGICAL-c4716-4497a33a13d1d1bff7e708337109922966367a071376582dbdabd83320fc560f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/92590B/92590B.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208408/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208408/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23709059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shan, Chao</creatorcontrib><creatorcontrib>Li, Xiaodan</creatorcontrib><creatorcontrib>Deng, Chenglin</creatorcontrib><creatorcontrib>Shang, Baodi</creatorcontrib><creatorcontrib>Xu, Linlin</creatorcontrib><creatorcontrib>Ye, Hanqing</creatorcontrib><creatorcontrib>Yuan, Zhiming</creatorcontrib><creatorcontrib>Zhang, Bo</creatorcontrib><title>Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase</title><title>Virologica Sinica</title><addtitle>Virol. Sin</addtitle><addtitle>Virologica Sinica</addtitle><description>We developed a Gaussia luciferase （Gluc） reporter replicon of West Nile virus （WNV） and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor （NITD008）, we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Line</subject><subject>Cricetinae</subject><subject>culture media</subject><subject>Culture Media - chemistry</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>drugs</subject><subject>Flavivirus</subject><subject>Gaussia</subject><subject>Genes, Reporter</subject><subject>luciferase</subject><subject>Luciferases - genetics</subject><subject>Luciferases - secretion</subject><subject>Medical Microbiology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Oncology</subject><subject>pathogenesis</subject><subject>replicon</subject><subject>Research Article</subject><subject>RNA</subject><subject>screening</subject><subject>Staining and Labeling - methods</subject><subject>translation (genetics)</subject><subject>Virology</subject><subject>virus replication</subject><subject>West Nile virus</subject><subject>West Nile virus - enzymology</subject><subject>West Nile virus - genetics</subject><subject>复制子</subject><subject>病毒复制</subject><subject>病毒抑制剂</subject><subject>细胞分泌</subject><subject>荧光素酶</subject><subject>药物开发</subject><subject>表征</subject><subject>西尼罗河病毒</subject><issn>1674-0769</issn><issn>1995-820X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQtRCIloUfwAXCBXEJjD9iJ5dKaCkFaQUSpcDNchIn6yprp_amlP56ZpVlgQucYuW9mXkfhDym8JICqFeJMlZADpTnnHOWqzvkmFZVkZcMvt3Ft1QiByWrI_IgpUsAyUrO75MjxhVUUFTHpHljr-0Qxo3128z4NluuTTTN1kZ3a7Yu-Cx02VebttkHN9jsi4tTyj7ZcXANYqc3Y7QpOd9n57aJdmvb7MxM-Mdkq6lxnY0m2YfkXmeGZB_tvwty8fb08_Jdvvp49n75epU3QlGZC1Epw7mhvKUtrbtOWQWoV1GoKsYqKblUBhTlShYla-vW1C3iDLqmkNDxBTmZ945TvbFtg5aiGfQY3cbEHzoYp_9GvFvrPlxrjKsUeGpBns8LvhvfGd_ryzBFj5L1bV-3NwyDBo4pIvHF_lIMVxOmozcuNXYYjLdhSpoKisUIyfj_qWiqLKkAgVQ6U5sYUoq2O0inoHeF67lwjTr0rnCtcObJn54PE78aRgKbCQkh39v429S_tj6dhzoTtOmjS_riHO0LQPeSFzutz_Za18H3V7j5cBptU-BK8J8jgcnG</recordid><startdate>201306</startdate><enddate>201306</enddate><creator>Shan, Chao</creator><creator>Li, Xiaodan</creator><creator>Deng, Chenglin</creator><creator>Shang, Baodi</creator><creator>Xu, Linlin</creator><creator>Ye, Hanqing</creator><creator>Yuan, Zhiming</creator><creator>Zhang, Bo</creator><general>Springer-Verlag</general><general>SP Wuhan Institute of Virology, CAS</general><general>Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China%Center for Emerging Infectious Diseases, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China</general><general>Center for Emerging Infectious Diseases, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>201306</creationdate><title>Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase</title><author>Shan, Chao ; Li, Xiaodan ; Deng, Chenglin ; Shang, Baodi ; Xu, Linlin ; Ye, Hanqing ; Yuan, Zhiming ; Zhang, Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4716-4497a33a13d1d1bff7e708337109922966367a071376582dbdabd83320fc560f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Line</topic><topic>Cricetinae</topic><topic>culture media</topic><topic>Culture Media - chemistry</topic><topic>Drug Evaluation, Preclinical - methods</topic><topic>drugs</topic><topic>Flavivirus</topic><topic>Gaussia</topic><topic>Genes, Reporter</topic><topic>luciferase</topic><topic>Luciferases - genetics</topic><topic>Luciferases - secretion</topic><topic>Medical Microbiology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Oncology</topic><topic>pathogenesis</topic><topic>replicon</topic><topic>Research Article</topic><topic>RNA</topic><topic>screening</topic><topic>Staining and Labeling - methods</topic><topic>translation (genetics)</topic><topic>Virology</topic><topic>virus replication</topic><topic>West Nile virus</topic><topic>West Nile virus - enzymology</topic><topic>West Nile virus - genetics</topic><topic>复制子</topic><topic>病毒复制</topic><topic>病毒抑制剂</topic><topic>细胞分泌</topic><topic>荧光素酶</topic><topic>药物开发</topic><topic>表征</topic><topic>西尼罗河病毒</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shan, Chao</creatorcontrib><creatorcontrib>Li, Xiaodan</creatorcontrib><creatorcontrib>Deng, Chenglin</creatorcontrib><creatorcontrib>Shang, Baodi</creatorcontrib><creatorcontrib>Xu, Linlin</creatorcontrib><creatorcontrib>Ye, Hanqing</creatorcontrib><creatorcontrib>Yuan, Zhiming</creatorcontrib><creatorcontrib>Zhang, Bo</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shan, Chao</au><au>Li, Xiaodan</au><au>Deng, Chenglin</au><au>Shang, Baodi</au><au>Xu, Linlin</au><au>Ye, Hanqing</au><au>Yuan, Zhiming</au><au>Zhang, Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase</atitle><jtitle>Virologica Sinica</jtitle><stitle>Virol. Sin</stitle><addtitle>Virologica Sinica</addtitle><date>2013-06</date><risdate>2013</risdate><volume>28</volume><issue>3</issue><spage>161</spage><epage>166</epage><pages>161-166</pages><issn>1674-0769</issn><eissn>1995-820X</eissn><abstract>We developed a Gaussia luciferase （Gluc） reporter replicon of West Nile virus （WNV） and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor （NITD008）, we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.</abstract><cop>Heidelberg</cop><pub>Springer-Verlag</pub><pmid>23709059</pmid><doi>10.1007/s12250-013-3332-7</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1674-0769 |
| ispartof | Virologica Sinica, 2013-06, Vol.28 (3), p.161-166 |
| issn | 1674-0769 1995-820X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8208408 |
| source | MEDLINE; SpringerNature Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Biochemistry Biomedical and Life Sciences Biomedicine Cell Line Cricetinae culture media Culture Media - chemistry Drug Evaluation, Preclinical - methods drugs Flavivirus Gaussia Genes, Reporter luciferase Luciferases - genetics Luciferases - secretion Medical Microbiology Microbial Genetics and Genomics Microbiology Oncology pathogenesis replicon Research Article RNA screening Staining and Labeling - methods translation (genetics) Virology virus replication West Nile virus West Nile virus - enzymology West Nile virus - genetics 复制子 病毒复制 病毒抑制剂 细胞分泌 荧光素酶 药物开发 表征 西尼罗河病毒 |
| title | Development and Characterization of West Nile Virus Replicon Expressing Secreted Gaussia Luciferase |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T22%3A07%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20and%20Characterization%20of%20West%20Nile%20Virus%20Replicon%20Expressing%20Secreted%20Gaussia%20Luciferase&rft.jtitle=Virologica%20Sinica&rft.au=Shan,%20Chao&rft.date=2013-06&rft.volume=28&rft.issue=3&rft.spage=161&rft.epage=166&rft.pages=161-166&rft.issn=1674-0769&rft.eissn=1995-820X&rft_id=info:doi/10.1007/s12250-013-3332-7&rft_dat=%3Cwanfang_jour_pubme%3Ezgbdx201303006%3C/wanfang_jour_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1367881404&rft_id=info:pmid/23709059&rft_cqvip_id=46210374&rft_wanfj_id=zgbdx201303006&rfr_iscdi=true |