Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress
Exercise intervention has become one of the most effective methods to prevent and treat osteoporosis, which is a common age‑related disease and seriously affects the health and quality of life of the elderly. However, the molecular mechanism remains to be elucidated. The present study demonstrated t...
Gespeichert in:
| Veröffentlicht in: | Molecular medicine reports 2021-08, Vol.24 (2), Article 561 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 2 |
| container_start_page | |
| container_title | Molecular medicine reports |
| container_volume | 24 |
| creator | Qiu, Yiyan Zhu, Guozheng Zeng, Canjun Yuan, Song Qian, Yuepeng Ye, Zelin Zhao, Shanwen Li, Runguang |
| description | Exercise intervention has become one of the most effective methods to prevent and treat osteoporosis, which is a common age‑related disease and seriously affects the health and quality of life of the elderly. However, the molecular mechanism remains to be elucidated. The present study demonstrated the exercise‑induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation in femur and tibia by establishing an animal exercise model using a treadmill exercise system. MicroRNA (miRNA/miR) and long non‑coding (lnc)RNA sequencing analyses identified 16 upregulated and two downregulated miRNAs in the exercise group, as well as 44 upregulated lncRNAs and 39 downregulated lncRNAs in the exercise group. There was increased expression of miR‑9942 and miR‑7704 in both the femur and tibia and an upregulation of miR‑30d, miR‑5100 and miR‑1260 in the femur of animals from the exercise group. In addition, four of the five most downregulated lncRNAs, including lncRNA MSTRG.2625, lncRNA MSTRG.1557, lncRNA MSTRG.691 and lncRNA MSTRG.7497, were demonstrated to be suppressed in both the femur and tibia after treadmill exercise. The results of the present study provided a valuable resource for further exploring the molecular mechanisms underlying the regulation of osteoporosis by exercise. |
| doi_str_mv | 10.3892/mmr.2021.12200 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8201655</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A669888499</galeid><sourcerecordid>A669888499</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-5bdb1576eb88975656c4760b8cb67a7fa1fed89727e3f9114d64576e8076eb953</originalsourceid><addsrcrecordid>eNptkc2KFDEUhYMozji6dSkBN266TVKVv43QDP7BMILoOqRSNz2RSjImVaI7X8FX9ElM9bSDwpBFLjnfOSQ5CD2lZNspzV7GWLaMMLqljBFyD51SqemmI6S_f5yZ1vIEPar1CyGCM64fopOup0T3rD9F7hK-z79__tpDgmLnkBOu8HWB5ELa4-xxDB8vdxXbNOIpucPsS464wdhDXMpBmsMQLF7SCAVHcFc2BWcnXOcCtT5GD7ydKjw57mfo85vXn87fbS4-vH1_vrvYuJ7LecOHcaBcChiU0pILLlwvBRmUG4S00lvqYWwKk9B5TWk_in7FFVk9mndn6NVN7vUyRBgdpLnYyVyXEG35YbIN5n8lhSuzz9-MYoQKvga8OAaU3P6gziaG6mCabIK8VMN4p5XUUoqGPr9B93YCE5LPLdGtuNkJoZVSvdaN2t5BtTVCDC4n8KGd32VwJddawN_enhKzFm5a4WYt3BwKb4Zn_775Fv_bcPcHJSKnsQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2539879776</pqid></control><display><type>article</type><title>Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress</title><source>Spandidos Publications Journals</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Qiu, Yiyan ; Zhu, Guozheng ; Zeng, Canjun ; Yuan, Song ; Qian, Yuepeng ; Ye, Zelin ; Zhao, Shanwen ; Li, Runguang</creator><creatorcontrib>Qiu, Yiyan ; Zhu, Guozheng ; Zeng, Canjun ; Yuan, Song ; Qian, Yuepeng ; Ye, Zelin ; Zhao, Shanwen ; Li, Runguang</creatorcontrib><description>Exercise intervention has become one of the most effective methods to prevent and treat osteoporosis, which is a common age‑related disease and seriously affects the health and quality of life of the elderly. However, the molecular mechanism remains to be elucidated. The present study demonstrated the exercise‑induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation in femur and tibia by establishing an animal exercise model using a treadmill exercise system. MicroRNA (miRNA/miR) and long non‑coding (lnc)RNA sequencing analyses identified 16 upregulated and two downregulated miRNAs in the exercise group, as well as 44 upregulated lncRNAs and 39 downregulated lncRNAs in the exercise group. There was increased expression of miR‑9942 and miR‑7704 in both the femur and tibia and an upregulation of miR‑30d, miR‑5100 and miR‑1260 in the femur of animals from the exercise group. In addition, four of the five most downregulated lncRNAs, including lncRNA MSTRG.2625, lncRNA MSTRG.1557, lncRNA MSTRG.691 and lncRNA MSTRG.7497, were demonstrated to be suppressed in both the femur and tibia after treadmill exercise. The results of the present study provided a valuable resource for further exploring the molecular mechanisms underlying the regulation of osteoporosis by exercise.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2021.12200</identifier><identifier>PMID: 34109424</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Adipogenesis - genetics ; Animals ; Biomarkers - metabolism ; Care and treatment ; Development and progression ; Down-Regulation - genetics ; Exercise ; Femur ; Femur - metabolism ; Gene Expression Regulation ; Genetic aspects ; High-Throughput Nucleotide Sequencing ; Male ; Medical research ; Medicine, Experimental ; MicroRNAs - genetics ; Models, Animal ; Osteogenesis - genetics ; Osteoporosis ; Physical Conditioning, Animal - physiology ; Physiological aspects ; PPAR gamma - metabolism ; Rats ; Rats, Sprague-Dawley ; RNA ; RNA sequencing ; RNA, Long Noncoding - genetics ; RNA, Messenger - genetics ; Signal Transduction - genetics ; Smad2 Protein - metabolism ; Stress (Physiology) ; Stress, Mechanical ; Tibia ; Tibia - metabolism ; Transforming Growth Factor beta1 - metabolism ; Up-Regulation - genetics</subject><ispartof>Molecular medicine reports, 2021-08, Vol.24 (2), Article 561</ispartof><rights>COPYRIGHT 2021 Spandidos Publications</rights><rights>Copyright: © Qiu et al. 2021</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-5bdb1576eb88975656c4760b8cb67a7fa1fed89727e3f9114d64576e8076eb953</citedby><cites>FETCH-LOGICAL-c457t-5bdb1576eb88975656c4760b8cb67a7fa1fed89727e3f9114d64576e8076eb953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,778,782,883,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34109424$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiu, Yiyan</creatorcontrib><creatorcontrib>Zhu, Guozheng</creatorcontrib><creatorcontrib>Zeng, Canjun</creatorcontrib><creatorcontrib>Yuan, Song</creatorcontrib><creatorcontrib>Qian, Yuepeng</creatorcontrib><creatorcontrib>Ye, Zelin</creatorcontrib><creatorcontrib>Zhao, Shanwen</creatorcontrib><creatorcontrib>Li, Runguang</creatorcontrib><title>Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Exercise intervention has become one of the most effective methods to prevent and treat osteoporosis, which is a common age‑related disease and seriously affects the health and quality of life of the elderly. However, the molecular mechanism remains to be elucidated. The present study demonstrated the exercise‑induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation in femur and tibia by establishing an animal exercise model using a treadmill exercise system. MicroRNA (miRNA/miR) and long non‑coding (lnc)RNA sequencing analyses identified 16 upregulated and two downregulated miRNAs in the exercise group, as well as 44 upregulated lncRNAs and 39 downregulated lncRNAs in the exercise group. There was increased expression of miR‑9942 and miR‑7704 in both the femur and tibia and an upregulation of miR‑30d, miR‑5100 and miR‑1260 in the femur of animals from the exercise group. In addition, four of the five most downregulated lncRNAs, including lncRNA MSTRG.2625, lncRNA MSTRG.1557, lncRNA MSTRG.691 and lncRNA MSTRG.7497, were demonstrated to be suppressed in both the femur and tibia after treadmill exercise. The results of the present study provided a valuable resource for further exploring the molecular mechanisms underlying the regulation of osteoporosis by exercise.</description><subject>Adipogenesis - genetics</subject><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Care and treatment</subject><subject>Development and progression</subject><subject>Down-Regulation - genetics</subject><subject>Exercise</subject><subject>Femur</subject><subject>Femur - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Genetic aspects</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>MicroRNAs - genetics</subject><subject>Models, Animal</subject><subject>Osteogenesis - genetics</subject><subject>Osteoporosis</subject><subject>Physical Conditioning, Animal - physiology</subject><subject>Physiological aspects</subject><subject>PPAR gamma - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA</subject><subject>RNA sequencing</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Signal Transduction - genetics</subject><subject>Smad2 Protein - metabolism</subject><subject>Stress (Physiology)</subject><subject>Stress, Mechanical</subject><subject>Tibia</subject><subject>Tibia - metabolism</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>Up-Regulation - genetics</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc2KFDEUhYMozji6dSkBN266TVKVv43QDP7BMILoOqRSNz2RSjImVaI7X8FX9ElM9bSDwpBFLjnfOSQ5CD2lZNspzV7GWLaMMLqljBFyD51SqemmI6S_f5yZ1vIEPar1CyGCM64fopOup0T3rD9F7hK-z79__tpDgmLnkBOu8HWB5ELa4-xxDB8vdxXbNOIpucPsS464wdhDXMpBmsMQLF7SCAVHcFc2BWcnXOcCtT5GD7ydKjw57mfo85vXn87fbS4-vH1_vrvYuJ7LecOHcaBcChiU0pILLlwvBRmUG4S00lvqYWwKk9B5TWk_in7FFVk9mndn6NVN7vUyRBgdpLnYyVyXEG35YbIN5n8lhSuzz9-MYoQKvga8OAaU3P6gziaG6mCabIK8VMN4p5XUUoqGPr9B93YCE5LPLdGtuNkJoZVSvdaN2t5BtTVCDC4n8KGd32VwJddawN_enhKzFm5a4WYt3BwKb4Zn_775Fv_bcPcHJSKnsQ</recordid><startdate>20210801</startdate><enddate>20210801</enddate><creator>Qiu, Yiyan</creator><creator>Zhu, Guozheng</creator><creator>Zeng, Canjun</creator><creator>Yuan, Song</creator><creator>Qian, Yuepeng</creator><creator>Ye, Zelin</creator><creator>Zhao, Shanwen</creator><creator>Li, Runguang</creator><general>Spandidos Publications</general><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210801</creationdate><title>Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress</title><author>Qiu, Yiyan ; Zhu, Guozheng ; Zeng, Canjun ; Yuan, Song ; Qian, Yuepeng ; Ye, Zelin ; Zhao, Shanwen ; Li, Runguang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-5bdb1576eb88975656c4760b8cb67a7fa1fed89727e3f9114d64576e8076eb953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adipogenesis - genetics</topic><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Care and treatment</topic><topic>Development and progression</topic><topic>Down-Regulation - genetics</topic><topic>Exercise</topic><topic>Femur</topic><topic>Femur - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Genetic aspects</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Male</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>MicroRNAs - genetics</topic><topic>Models, Animal</topic><topic>Osteogenesis - genetics</topic><topic>Osteoporosis</topic><topic>Physical Conditioning, Animal - physiology</topic><topic>Physiological aspects</topic><topic>PPAR gamma - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA</topic><topic>RNA sequencing</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Signal Transduction - genetics</topic><topic>Smad2 Protein - metabolism</topic><topic>Stress (Physiology)</topic><topic>Stress, Mechanical</topic><topic>Tibia</topic><topic>Tibia - metabolism</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><topic>Up-Regulation - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Qiu, Yiyan</creatorcontrib><creatorcontrib>Zhu, Guozheng</creatorcontrib><creatorcontrib>Zeng, Canjun</creatorcontrib><creatorcontrib>Yuan, Song</creatorcontrib><creatorcontrib>Qian, Yuepeng</creatorcontrib><creatorcontrib>Ye, Zelin</creatorcontrib><creatorcontrib>Zhao, Shanwen</creatorcontrib><creatorcontrib>Li, Runguang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiu, Yiyan</au><au>Zhu, Guozheng</au><au>Zeng, Canjun</au><au>Yuan, Song</au><au>Qian, Yuepeng</au><au>Ye, Zelin</au><au>Zhao, Shanwen</au><au>Li, Runguang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2021-08-01</date><risdate>2021</risdate><volume>24</volume><issue>2</issue><artnum>561</artnum><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>Exercise intervention has become one of the most effective methods to prevent and treat osteoporosis, which is a common age‑related disease and seriously affects the health and quality of life of the elderly. However, the molecular mechanism remains to be elucidated. The present study demonstrated the exercise‑induced promotion of osteogenic differentiation and inhibition of adipogenic differentiation in femur and tibia by establishing an animal exercise model using a treadmill exercise system. MicroRNA (miRNA/miR) and long non‑coding (lnc)RNA sequencing analyses identified 16 upregulated and two downregulated miRNAs in the exercise group, as well as 44 upregulated lncRNAs and 39 downregulated lncRNAs in the exercise group. There was increased expression of miR‑9942 and miR‑7704 in both the femur and tibia and an upregulation of miR‑30d, miR‑5100 and miR‑1260 in the femur of animals from the exercise group. In addition, four of the five most downregulated lncRNAs, including lncRNA MSTRG.2625, lncRNA MSTRG.1557, lncRNA MSTRG.691 and lncRNA MSTRG.7497, were demonstrated to be suppressed in both the femur and tibia after treadmill exercise. The results of the present study provided a valuable resource for further exploring the molecular mechanisms underlying the regulation of osteoporosis by exercise.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>34109424</pmid><doi>10.3892/mmr.2021.12200</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1791-2997 |
| ispartof | Molecular medicine reports, 2021-08, Vol.24 (2), Article 561 |
| issn | 1791-2997 1791-3004 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8201655 |
| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adipogenesis - genetics Animals Biomarkers - metabolism Care and treatment Development and progression Down-Regulation - genetics Exercise Femur Femur - metabolism Gene Expression Regulation Genetic aspects High-Throughput Nucleotide Sequencing Male Medical research Medicine, Experimental MicroRNAs - genetics Models, Animal Osteogenesis - genetics Osteoporosis Physical Conditioning, Animal - physiology Physiological aspects PPAR gamma - metabolism Rats Rats, Sprague-Dawley RNA RNA sequencing RNA, Long Noncoding - genetics RNA, Messenger - genetics Signal Transduction - genetics Smad2 Protein - metabolism Stress (Physiology) Stress, Mechanical Tibia Tibia - metabolism Transforming Growth Factor beta1 - metabolism Up-Regulation - genetics |
| title | Next‑generation sequencing of miRNAs and lncRNAs from rat femur and tibia under mechanical stress |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T11%3A44%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Next%E2%80%91generation%20sequencing%20of%20miRNAs%20and%20lncRNAs%20from%20rat%20femur%20and%20tibia%20under%20mechanical%20stress&rft.jtitle=Molecular%20medicine%20reports&rft.au=Qiu,%20Yiyan&rft.date=2021-08-01&rft.volume=24&rft.issue=2&rft.artnum=561&rft.issn=1791-2997&rft.eissn=1791-3004&rft_id=info:doi/10.3892/mmr.2021.12200&rft_dat=%3Cgale_pubme%3EA669888499%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2539879776&rft_id=info:pmid/34109424&rft_galeid=A669888499&rfr_iscdi=true |