Isolation and characterization of glacier VMY22, a novel lytic cold-active bacteriophage of Bacillus cereus
As a unique ecological system with low temperature and low nutrient levels, glaciers are considered a "living fossil" for the research of evolution. In this work, a lytic cold-active bacteriophage designated VMY22 against Bacillus cereus MYB41-22 was isolated from Mingyong Glacier...
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| Veröffentlicht in: | Virologica Sinica 2015-02, Vol.30 (1), p.52-58 |
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| creator | Ji, Xiuling Zhang, Chunjing Fang, Yuan Zhang, Qi Lin, Lianbing Tang, Bing Wei, Yunlin |
| description | As a unique ecological system with low temperature and low nutrient levels, glaciers are considered a "living fossil" for the research of evolution. In this work, a lytic cold-active bacteriophage designated VMY22 against Bacillus cereus MYB41-22 was isolated from Mingyong Glacier in China, and its characteristics were studied. Electron microscopy revealed that VMY22 has an icosahedral head(59.2 nm in length, 31.9 nm in width) and a tail(43.2 nm in length). Bacteriophage VMY22 was classified as a Podoviridae with an approximate genome size of 18 to 20 kb. A one-step growth curve revealed that the latent and the burst periods were 70 and 70 min, respectively, with an average burst size of 78 bacteriophage particles per infected cell. The pH and thermal stability of bacteriophage VMY22 were also investigated. The maximum stability of the bacteriophage was observed to be at pH 8.0 and it was comparatively stable at p H 5.0–9.0. As VMY22 is a cold-active bacteriophage with low production temperature, its characterization and the relationship between MYB41-22 and Bacillus cereus bacteriophage deserve further study. |
| doi_str_mv | 10.1007/s12250-014-3529-4 |
| format | Article |
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In this work, a lytic cold-active bacteriophage designated VMY22 against Bacillus cereus MYB41-22 was isolated from Mingyong Glacier in China, and its characteristics were studied. Electron microscopy revealed that VMY22 has an icosahedral head(59.2 nm in length, 31.9 nm in width) and a tail(43.2 nm in length). Bacteriophage VMY22 was classified as a Podoviridae with an approximate genome size of 18 to 20 kb. A one-step growth curve revealed that the latent and the burst periods were 70 and 70 min, respectively, with an average burst size of 78 bacteriophage particles per infected cell. The pH and thermal stability of bacteriophage VMY22 were also investigated. The maximum stability of the bacteriophage was observed to be at pH 8.0 and it was comparatively stable at p H 5.0–9.0. As VMY22 is a cold-active bacteriophage with low production temperature, its characterization and the relationship between MYB41-22 and Bacillus cereus bacteriophage deserve further study.</description><identifier>ISSN: 1674-0769</identifier><identifier>EISSN: 1995-820X</identifier><identifier>DOI: 10.1007/s12250-014-3529-4</identifier><identifier>PMID: 25680445</identifier><language>eng</language><publisher>Heidelberg: Wuhan Institute of Virology, CAS</publisher><subject>Bacillus ; Bacillus cereus ; Bacillus cereus - virology ; Bacteriophages - classification ; Bacteriophages - genetics ; Bacteriophages - isolation & purification ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; cereus ; characterization ; China ; Cold Temperature ; cold-active ; Genome, Viral ; Ice Cover - virology ; lytic ; Medical Microbiology ; Microbial Genetics and Genomics ; Microbiology ; Molecular Sequence Data ; Oncology ; phage ; Phylogeny ; Podoviridae ; Podoviridae - classification ; Podoviridae - genetics ; Podoviridae - isolation & purification ; Research Article ; Virology</subject><ispartof>Virologica Sinica, 2015-02, Vol.30 (1), p.52-58</ispartof><rights>Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c519t-e001dd716a91e357c2cc01a322df5d858778efb399628abf8a9a56ab61886d3c3</citedby><cites>FETCH-LOGICAL-c519t-e001dd716a91e357c2cc01a322df5d858778efb399628abf8a9a56ab61886d3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/92590B/92590B.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200904/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200904/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,41467,42536,51297,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25680445$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ji, Xiuling</creatorcontrib><creatorcontrib>Zhang, Chunjing</creatorcontrib><creatorcontrib>Fang, Yuan</creatorcontrib><creatorcontrib>Zhang, Qi</creatorcontrib><creatorcontrib>Lin, Lianbing</creatorcontrib><creatorcontrib>Tang, Bing</creatorcontrib><creatorcontrib>Wei, Yunlin</creatorcontrib><title>Isolation and characterization of glacier VMY22, a novel lytic cold-active bacteriophage of Bacillus cereus</title><title>Virologica Sinica</title><addtitle>Virol. Sin</addtitle><addtitle>中国病毒学(英文版)</addtitle><description>As a unique ecological system with low temperature and low nutrient levels, glaciers are considered a &quot;living fossil&quot; for the research of evolution. In this work, a lytic cold-active bacteriophage designated VMY22 against Bacillus cereus MYB41-22 was isolated from Mingyong Glacier in China, and its characteristics were studied. Electron microscopy revealed that VMY22 has an icosahedral head(59.2 nm in length, 31.9 nm in width) and a tail(43.2 nm in length). Bacteriophage VMY22 was classified as a Podoviridae with an approximate genome size of 18 to 20 kb. A one-step growth curve revealed that the latent and the burst periods were 70 and 70 min, respectively, with an average burst size of 78 bacteriophage particles per infected cell. The pH and thermal stability of bacteriophage VMY22 were also investigated. The maximum stability of the bacteriophage was observed to be at pH 8.0 and it was comparatively stable at p H 5.0–9.0. As VMY22 is a cold-active bacteriophage with low production temperature, its characterization and the relationship between MYB41-22 and Bacillus cereus bacteriophage deserve further study.</description><subject>Bacillus</subject><subject>Bacillus cereus</subject><subject>Bacillus cereus - virology</subject><subject>Bacteriophages - classification</subject><subject>Bacteriophages - genetics</subject><subject>Bacteriophages - isolation & purification</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>cereus</subject><subject>characterization</subject><subject>China</subject><subject>Cold Temperature</subject><subject>cold-active</subject><subject>Genome, Viral</subject><subject>Ice Cover - virology</subject><subject>lytic</subject><subject>Medical Microbiology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Oncology</subject><subject>phage</subject><subject>Phylogeny</subject><subject>Podoviridae</subject><subject>Podoviridae - classification</subject><subject>Podoviridae - genetics</subject><subject>Podoviridae - isolation & purification</subject><subject>Research Article</subject><subject>Virology</subject><issn>1674-0769</issn><issn>1995-820X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU1v1DAUjBCIlsIP4IJ85IDh-TP2BYlWFCoVcQEEJ8txnGyKN97ayUrtr8dRlhVcEAfbT34zo3lvquo5gdcEoH6TCaUCMBCOmaAa8wfVKdFaYEXh-8NSy5pjqKU-qZ7kfAMgqWLscXVChVTAuTitfl7lGOw0xBHZsUVuY5N1k0_D_foZO9QH6waf0LdPPyh9hSwa494HFO6mwSEXQ4sLY9h71KzMuNvY3i_M80IMYc7I-eTn_LR61NmQ_bPDe1Z9vXz_5eIjvv784eri3TV2gugJewDStjWRVhPPRO2oc0Aso7TtRKuEqmvlu4ZpXcaxTaestkLaRhKlZMscO6verrq7udn61vlxSjaYXRq2Nt2ZaAfzd2ccNqaPe1PWBhp4EXh5EEjxdvZ5MtshOx-CHX2csyl7lYxLDfo_oEIIygllBUpWqEsx5-S7oyMCZsnTrHmakqdZ8jSLkxd_jnJk_A6wAOgKyKU19j6Zmzinsaz3n6rs4GQTx_628I7CGqSUSikBXHEt2HKXo0TNfgEMcLtu</recordid><startdate>20150201</startdate><enddate>20150201</enddate><creator>Ji, Xiuling</creator><creator>Zhang, Chunjing</creator><creator>Fang, Yuan</creator><creator>Zhang, Qi</creator><creator>Lin, Lianbing</creator><creator>Tang, Bing</creator><creator>Wei, Yunlin</creator><general>Wuhan Institute of Virology, CAS</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>20150201</creationdate><title>Isolation and characterization of glacier VMY22, a novel lytic cold-active bacteriophage of Bacillus cereus</title><author>Ji, Xiuling ; Zhang, Chunjing ; Fang, Yuan ; Zhang, Qi ; Lin, Lianbing ; Tang, Bing ; Wei, Yunlin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c519t-e001dd716a91e357c2cc01a322df5d858778efb399628abf8a9a56ab61886d3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bacillus</topic><topic>Bacillus cereus</topic><topic>Bacillus cereus - virology</topic><topic>Bacteriophages - classification</topic><topic>Bacteriophages - genetics</topic><topic>Bacteriophages - isolation & purification</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>cereus</topic><topic>characterization</topic><topic>China</topic><topic>Cold Temperature</topic><topic>cold-active</topic><topic>Genome, Viral</topic><topic>Ice Cover - virology</topic><topic>lytic</topic><topic>Medical Microbiology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Oncology</topic><topic>phage</topic><topic>Phylogeny</topic><topic>Podoviridae</topic><topic>Podoviridae - classification</topic><topic>Podoviridae - genetics</topic><topic>Podoviridae - isolation & purification</topic><topic>Research Article</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ji, Xiuling</creatorcontrib><creatorcontrib>Zhang, Chunjing</creatorcontrib><creatorcontrib>Fang, Yuan</creatorcontrib><creatorcontrib>Zhang, Qi</creatorcontrib><creatorcontrib>Lin, Lianbing</creatorcontrib><creatorcontrib>Tang, Bing</creatorcontrib><creatorcontrib>Wei, Yunlin</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Virologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ji, Xiuling</au><au>Zhang, Chunjing</au><au>Fang, Yuan</au><au>Zhang, Qi</au><au>Lin, Lianbing</au><au>Tang, Bing</au><au>Wei, Yunlin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of glacier VMY22, a novel lytic cold-active bacteriophage of Bacillus cereus</atitle><jtitle>Virologica Sinica</jtitle><stitle>Virol. Sin</stitle><addtitle>中国病毒学(英文版)</addtitle><date>2015-02-01</date><risdate>2015</risdate><volume>30</volume><issue>1</issue><spage>52</spage><epage>58</epage><pages>52-58</pages><issn>1674-0769</issn><eissn>1995-820X</eissn><abstract>As a unique ecological system with low temperature and low nutrient levels, glaciers are considered a &quot;living fossil&quot; for the research of evolution. In this work, a lytic cold-active bacteriophage designated VMY22 against Bacillus cereus MYB41-22 was isolated from Mingyong Glacier in China, and its characteristics were studied. Electron microscopy revealed that VMY22 has an icosahedral head(59.2 nm in length, 31.9 nm in width) and a tail(43.2 nm in length). Bacteriophage VMY22 was classified as a Podoviridae with an approximate genome size of 18 to 20 kb. A one-step growth curve revealed that the latent and the burst periods were 70 and 70 min, respectively, with an average burst size of 78 bacteriophage particles per infected cell. The pH and thermal stability of bacteriophage VMY22 were also investigated. The maximum stability of the bacteriophage was observed to be at pH 8.0 and it was comparatively stable at p H 5.0–9.0. As VMY22 is a cold-active bacteriophage with low production temperature, its characterization and the relationship between MYB41-22 and Bacillus cereus bacteriophage deserve further study.</abstract><cop>Heidelberg</cop><pub>Wuhan Institute of Virology, CAS</pub><pmid>25680445</pmid><doi>10.1007/s12250-014-3529-4</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Virologica Sinica, 2015-02, Vol.30 (1), p.52-58 |
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| subjects | Bacillus Bacillus cereus Bacillus cereus - virology Bacteriophages - classification Bacteriophages - genetics Bacteriophages - isolation & purification Biochemistry Biomedical and Life Sciences Biomedicine cereus characterization China Cold Temperature cold-active Genome, Viral Ice Cover - virology lytic Medical Microbiology Microbial Genetics and Genomics Microbiology Molecular Sequence Data Oncology phage Phylogeny Podoviridae Podoviridae - classification Podoviridae - genetics Podoviridae - isolation & purification Research Article Virology |
| title | Isolation and characterization of glacier VMY22, a novel lytic cold-active bacteriophage of Bacillus cereus |
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