Native Spider Silk-Based Antimicrobial Hydrogels for Biomedical Applications
Novel antimicrobial natural polymeric hybrid hydrogels based on hyaluronic acid (HA) and spider silk (Ss) were prepared using the chemical crosslinking method. The effects of the component ratios on the hydrogel characteristics were observed parallel to the primary physicochemical characterization o...
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| Veröffentlicht in: | Polymers 2021-05, Vol.13 (11), p.1796 |
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| creator | Withanage, Sinith Savin, Artemii Nikolaeva, Valeria Kiseleva, Aleksandra Dukhinova, Marina Krivoshapkin, Pavel Krivoshapkina, Elena |
| description | Novel antimicrobial natural polymeric hybrid hydrogels based on hyaluronic acid (HA) and spider silk (Ss) were prepared using the chemical crosslinking method. The effects of the component ratios on the hydrogel characteristics were observed parallel to the primary physicochemical characterization of the hydrogels with scanning electron microscopic imaging, Fourier-transform infrared spectroscopy, and contact angle measurements, which confirmed the successful crosslinking, regular porous structure, exact composition, and hydrophilic properties of hyaluronic acid/spider silk-based hydrogels. Further characterizations of the hydrogels were performed with the swelling degree, enzymatic degradability, viscosity, conductivity, and shrinking ability tests. The hyaluronic acid/spider silk-based hydrogels do not show drastic cytotoxicity over human postnatal fibroblasts (HPF). Hydrogels show extraordinary antimicrobial ability on both gram-negative and gram-positive bacteria. These hydrogels could be an excellent alternative that aids in overcoming antimicrobial drug resistance, which is considered to be one of the major global problems in the biomedical industry. Hyaluronic acid/spider silk-based hydrogels are a promising material for collaborated antimicrobial and anti-inflammatory drug delivery systems for external use. The rheological properties of the hydrogels show shear-thinning properties, which suggest that the hydrogels could be applied in 3D printing, such as in the 3D printing of antimicrobial surgical meshes. |
| doi_str_mv | 10.3390/polym13111796 |
| format | Article |
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The effects of the component ratios on the hydrogel characteristics were observed parallel to the primary physicochemical characterization of the hydrogels with scanning electron microscopic imaging, Fourier-transform infrared spectroscopy, and contact angle measurements, which confirmed the successful crosslinking, regular porous structure, exact composition, and hydrophilic properties of hyaluronic acid/spider silk-based hydrogels. Further characterizations of the hydrogels were performed with the swelling degree, enzymatic degradability, viscosity, conductivity, and shrinking ability tests. The hyaluronic acid/spider silk-based hydrogels do not show drastic cytotoxicity over human postnatal fibroblasts (HPF). Hydrogels show extraordinary antimicrobial ability on both gram-negative and gram-positive bacteria. These hydrogels could be an excellent alternative that aids in overcoming antimicrobial drug resistance, which is considered to be one of the major global problems in the biomedical industry. Hyaluronic acid/spider silk-based hydrogels are a promising material for collaborated antimicrobial and anti-inflammatory drug delivery systems for external use. The rheological properties of the hydrogels show shear-thinning properties, which suggest that the hydrogels could be applied in 3D printing, such as in the 3D printing of antimicrobial surgical meshes.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym13111796</identifier><identifier>PMID: 34072375</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animal behavior ; Antiinfectives and antibacterials ; Antimicrobial agents ; Biocompatibility ; Biomedical materials ; Biopolymers ; Collagen ; Contact angle ; Crosslinking ; Drug delivery systems ; Fibroblasts ; Fourier transforms ; Hyaluronic acid ; Hydrogels ; Infrared imaging ; Investigations ; Pain ; Polymers ; Proteins ; Rheological properties ; Shear thinning (liquids) ; Silk ; Skin ; Spiders ; Surgical mesh ; Three dimensional printing ; Toxicity</subject><ispartof>Polymers, 2021-05, Vol.13 (11), p.1796</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-871eb2511922786dfc8463b07340bd79ec4dd35c854b92c6c7b4e14c56bb469f3</citedby><cites>FETCH-LOGICAL-c345t-871eb2511922786dfc8463b07340bd79ec4dd35c854b92c6c7b4e14c56bb469f3</cites><orcidid>0000-0001-6981-5134 ; 0000-0002-1121-4974 ; 0000-0002-3457-6791</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198725/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198725/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34072375$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Withanage, Sinith</creatorcontrib><creatorcontrib>Savin, Artemii</creatorcontrib><creatorcontrib>Nikolaeva, Valeria</creatorcontrib><creatorcontrib>Kiseleva, Aleksandra</creatorcontrib><creatorcontrib>Dukhinova, Marina</creatorcontrib><creatorcontrib>Krivoshapkin, Pavel</creatorcontrib><creatorcontrib>Krivoshapkina, Elena</creatorcontrib><title>Native Spider Silk-Based Antimicrobial Hydrogels for Biomedical Applications</title><title>Polymers</title><addtitle>Polymers (Basel)</addtitle><description>Novel antimicrobial natural polymeric hybrid hydrogels based on hyaluronic acid (HA) and spider silk (Ss) were prepared using the chemical crosslinking method. The effects of the component ratios on the hydrogel characteristics were observed parallel to the primary physicochemical characterization of the hydrogels with scanning electron microscopic imaging, Fourier-transform infrared spectroscopy, and contact angle measurements, which confirmed the successful crosslinking, regular porous structure, exact composition, and hydrophilic properties of hyaluronic acid/spider silk-based hydrogels. Further characterizations of the hydrogels were performed with the swelling degree, enzymatic degradability, viscosity, conductivity, and shrinking ability tests. The hyaluronic acid/spider silk-based hydrogels do not show drastic cytotoxicity over human postnatal fibroblasts (HPF). Hydrogels show extraordinary antimicrobial ability on both gram-negative and gram-positive bacteria. These hydrogels could be an excellent alternative that aids in overcoming antimicrobial drug resistance, which is considered to be one of the major global problems in the biomedical industry. Hyaluronic acid/spider silk-based hydrogels are a promising material for collaborated antimicrobial and anti-inflammatory drug delivery systems for external use. The rheological properties of the hydrogels show shear-thinning properties, which suggest that the hydrogels could be applied in 3D printing, such as in the 3D printing of antimicrobial surgical meshes.</description><subject>Animal behavior</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Biopolymers</subject><subject>Collagen</subject><subject>Contact angle</subject><subject>Crosslinking</subject><subject>Drug delivery systems</subject><subject>Fibroblasts</subject><subject>Fourier transforms</subject><subject>Hyaluronic acid</subject><subject>Hydrogels</subject><subject>Infrared imaging</subject><subject>Investigations</subject><subject>Pain</subject><subject>Polymers</subject><subject>Proteins</subject><subject>Rheological properties</subject><subject>Shear thinning (liquids)</subject><subject>Silk</subject><subject>Skin</subject><subject>Spiders</subject><subject>Surgical mesh</subject><subject>Three dimensional printing</subject><subject>Toxicity</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkc1LAzEQxYMoWqpHr7LgxctqvjbZXIS2qBWKHtRz2GSzNZrdrMm20P_eiFWqc5mB-fF4Mw-AUwQvCRHwqvdu0yKCEOKC7YERhpzklDC4vzMfgZMY32AqWjCG-CE4IhRyTHgxAouHarBrkz31tjYhe7LuPZ9W0dTZpBtsa3XwylYum2_q4JfGxazxIZta35ra6rSY9L1Lw2B9F4_BQVO5aE62fQxebm-eZ_N88Xh3P5ssck1oMeQlR0bhAiGBMS9Z3eiSMqKSXwpVzYXRtK5JocuCKoE101xRg6gumFKUiYaMwfW3br9SyYc23RAqJ_tg2ypspK-s_Lvp7Ktc-rUskSg5LpLAxVYg-I-ViYNsbdTGuaozfhVlQliylP6V0PN_6JtfhS6d90UJwTDENFH5N5X-FWMwza8ZBOVXVPJPVIk_273gl_4JhnwC3oCPrQ</recordid><startdate>20210529</startdate><enddate>20210529</enddate><creator>Withanage, Sinith</creator><creator>Savin, Artemii</creator><creator>Nikolaeva, Valeria</creator><creator>Kiseleva, Aleksandra</creator><creator>Dukhinova, Marina</creator><creator>Krivoshapkin, Pavel</creator><creator>Krivoshapkina, Elena</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6981-5134</orcidid><orcidid>https://orcid.org/0000-0002-1121-4974</orcidid><orcidid>https://orcid.org/0000-0002-3457-6791</orcidid></search><sort><creationdate>20210529</creationdate><title>Native Spider Silk-Based Antimicrobial Hydrogels for Biomedical Applications</title><author>Withanage, Sinith ; 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The effects of the component ratios on the hydrogel characteristics were observed parallel to the primary physicochemical characterization of the hydrogels with scanning electron microscopic imaging, Fourier-transform infrared spectroscopy, and contact angle measurements, which confirmed the successful crosslinking, regular porous structure, exact composition, and hydrophilic properties of hyaluronic acid/spider silk-based hydrogels. Further characterizations of the hydrogels were performed with the swelling degree, enzymatic degradability, viscosity, conductivity, and shrinking ability tests. The hyaluronic acid/spider silk-based hydrogels do not show drastic cytotoxicity over human postnatal fibroblasts (HPF). Hydrogels show extraordinary antimicrobial ability on both gram-negative and gram-positive bacteria. These hydrogels could be an excellent alternative that aids in overcoming antimicrobial drug resistance, which is considered to be one of the major global problems in the biomedical industry. Hyaluronic acid/spider silk-based hydrogels are a promising material for collaborated antimicrobial and anti-inflammatory drug delivery systems for external use. The rheological properties of the hydrogels show shear-thinning properties, which suggest that the hydrogels could be applied in 3D printing, such as in the 3D printing of antimicrobial surgical meshes.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>34072375</pmid><doi>10.3390/polym13111796</doi><orcidid>https://orcid.org/0000-0001-6981-5134</orcidid><orcidid>https://orcid.org/0000-0002-1121-4974</orcidid><orcidid>https://orcid.org/0000-0002-3457-6791</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Animal behavior Antiinfectives and antibacterials Antimicrobial agents Biocompatibility Biomedical materials Biopolymers Collagen Contact angle Crosslinking Drug delivery systems Fibroblasts Fourier transforms Hyaluronic acid Hydrogels Infrared imaging Investigations Pain Polymers Proteins Rheological properties Shear thinning (liquids) Silk Skin Spiders Surgical mesh Three dimensional printing Toxicity |
| title | Native Spider Silk-Based Antimicrobial Hydrogels for Biomedical Applications |
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