Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering
Decellularized vascular grafts are useful for the construction of biological small-diameter tissue-engineered vascular grafts (≤6 mm). Traditional chemical decellularization requires a long treatment time, which may damage the structure and alter the mechanical properties. Decellularization using so...
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| Veröffentlicht in: | Polymers 2021-05, Vol.13 (11), p.1699 |
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| creator | Lin, Chih-Hsun Hsia, Kai Su, Chih-Kuan Chen, Chien-Chin Yeh, Chang-Ching Ma, Hsu Lu, Jen-Her |
| description | Decellularized vascular grafts are useful for the construction of biological small-diameter tissue-engineered vascular grafts (≤6 mm). Traditional chemical decellularization requires a long treatment time, which may damage the structure and alter the mechanical properties. Decellularization using sonication is expected to solve this problem. The aim of this study was to develop an effective decellularization method using ultrasound followed by washing. Different power values of sonication at 40 kHz were tested for 2, 4, and 8 h followed by a washing procedure. The efficacy of sonication of decellularized human umbilical artery (sDHUA) was evaluated via DNA content, histological staining, mechanical properties, and biocompatibility. The sDHUAs were further implanted into rats for up to 90 days and magnetic resonance angiography (MRA) was performed for the implanted grafts. The results demonstrated that treatment of human umbilical artery (HUA) by sonication at ultrasonic power of 204 W for 4 h followed by washing for 24 h in 2% SDS buffer could eliminate more than 90% of cells and retain similar mechanical properties of the HUA. Recellularization was assessed by scanning electron microscopy (SEM), which indicated that sDHUA provided niches for human umbilical vein endothelial cells (HUVECs) to reside, indicating in vitro cytocompatibility. Further implantation tests also indicated the fitness of the sonication-treated HUA as a scaffold for small-caliber tissue engineering vascular grafts. |
| doi_str_mv | 10.3390/polym13111699 |
| format | Article |
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Traditional chemical decellularization requires a long treatment time, which may damage the structure and alter the mechanical properties. Decellularization using sonication is expected to solve this problem. The aim of this study was to develop an effective decellularization method using ultrasound followed by washing. Different power values of sonication at 40 kHz were tested for 2, 4, and 8 h followed by a washing procedure. The efficacy of sonication of decellularized human umbilical artery (sDHUA) was evaluated via DNA content, histological staining, mechanical properties, and biocompatibility. The sDHUAs were further implanted into rats for up to 90 days and magnetic resonance angiography (MRA) was performed for the implanted grafts. The results demonstrated that treatment of human umbilical artery (HUA) by sonication at ultrasonic power of 204 W for 4 h followed by washing for 24 h in 2% SDS buffer could eliminate more than 90% of cells and retain similar mechanical properties of the HUA. Recellularization was assessed by scanning electron microscopy (SEM), which indicated that sDHUA provided niches for human umbilical vein endothelial cells (HUVECs) to reside, indicating in vitro cytocompatibility. Further implantation tests also indicated the fitness of the sonication-treated HUA as a scaffold for small-caliber tissue engineering vascular grafts.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym13111699</identifier><identifier>PMID: 34067495</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Angiography ; Biocompatibility ; Biomechanics ; Cavitation ; Collagen ; Coronary vessels ; Endothelial cells ; Extracellular matrix ; Grafting ; Histology ; Hospitals ; Laboratory animals ; Life sciences ; Magnetic resonance ; Mechanical properties ; Tissue engineering ; Ultrasonic testing ; Vascular tissue ; Veins & arteries ; Washing</subject><ispartof>Polymers, 2021-05, Vol.13 (11), p.1699</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-4d91680289aabb5ee864ec6068aa9c9a707369a850881c90717d4590d17ab2613</citedby><cites>FETCH-LOGICAL-c415t-4d91680289aabb5ee864ec6068aa9c9a707369a850881c90717d4590d17ab2613</cites><orcidid>0000-0002-9771-5816 ; 0000-0001-9599-0884 ; 0000-0003-0690-7503 ; 0000-0003-2056-3065</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196986/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196986/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34067495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Chih-Hsun</creatorcontrib><creatorcontrib>Hsia, Kai</creatorcontrib><creatorcontrib>Su, Chih-Kuan</creatorcontrib><creatorcontrib>Chen, Chien-Chin</creatorcontrib><creatorcontrib>Yeh, Chang-Ching</creatorcontrib><creatorcontrib>Ma, Hsu</creatorcontrib><creatorcontrib>Lu, Jen-Her</creatorcontrib><title>Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering</title><title>Polymers</title><addtitle>Polymers (Basel)</addtitle><description>Decellularized vascular grafts are useful for the construction of biological small-diameter tissue-engineered vascular grafts (≤6 mm). Traditional chemical decellularization requires a long treatment time, which may damage the structure and alter the mechanical properties. Decellularization using sonication is expected to solve this problem. The aim of this study was to develop an effective decellularization method using ultrasound followed by washing. Different power values of sonication at 40 kHz were tested for 2, 4, and 8 h followed by a washing procedure. The efficacy of sonication of decellularized human umbilical artery (sDHUA) was evaluated via DNA content, histological staining, mechanical properties, and biocompatibility. The sDHUAs were further implanted into rats for up to 90 days and magnetic resonance angiography (MRA) was performed for the implanted grafts. The results demonstrated that treatment of human umbilical artery (HUA) by sonication at ultrasonic power of 204 W for 4 h followed by washing for 24 h in 2% SDS buffer could eliminate more than 90% of cells and retain similar mechanical properties of the HUA. Recellularization was assessed by scanning electron microscopy (SEM), which indicated that sDHUA provided niches for human umbilical vein endothelial cells (HUVECs) to reside, indicating in vitro cytocompatibility. Further implantation tests also indicated the fitness of the sonication-treated HUA as a scaffold for small-caliber tissue engineering vascular grafts.</description><subject>Angiography</subject><subject>Biocompatibility</subject><subject>Biomechanics</subject><subject>Cavitation</subject><subject>Collagen</subject><subject>Coronary vessels</subject><subject>Endothelial cells</subject><subject>Extracellular matrix</subject><subject>Grafting</subject><subject>Histology</subject><subject>Hospitals</subject><subject>Laboratory animals</subject><subject>Life sciences</subject><subject>Magnetic resonance</subject><subject>Mechanical properties</subject><subject>Tissue engineering</subject><subject>Ultrasonic testing</subject><subject>Vascular tissue</subject><subject>Veins & arteries</subject><subject>Washing</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkTFvFDEQhS0EIlFISYss0dAs2Gev126QTkdCkIIoktBas965iyOvfdi7iOPXx5eEKMHNWJpvnubNI-QtZx-FMOzTNoXdyAXnXBnzghwuWCcaKRR7-eR_QI5LuWH1yVYp3r0mB0Iy1UnTHpI_Fyl6B5NPsVmW4suEA_2O03Ua6Dpl-gUdhjAHyP7vHUXTmp7NI0R6NfY-1NlAl3nCvLvjL0YIoVlB8D1m-hOK28_SS1_KjPQkbnxEzD5u3pBXawgFjx_qEbk6PblcnTXnP75-Wy3PGyd5OzVyMFxpttAGoO9bRK0kOsWUBjDOQFddKgO6ZVpzZ1jHu0G2hg28g36huDgin-91t3M_4uAwThmC3WY_Qt7ZBN4-70R_bTfpt9XcKKNVFfjwIJDTrxnLZEdf9keBiGkudtEKJZVuhano-__QmzTnWO3tKWPq2lJUqrmnXE6lZFw_LsOZ3cdqn8Va-XdPHTzS_0IUt_23n-A</recordid><startdate>20210522</startdate><enddate>20210522</enddate><creator>Lin, Chih-Hsun</creator><creator>Hsia, Kai</creator><creator>Su, Chih-Kuan</creator><creator>Chen, Chien-Chin</creator><creator>Yeh, Chang-Ching</creator><creator>Ma, Hsu</creator><creator>Lu, Jen-Her</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9771-5816</orcidid><orcidid>https://orcid.org/0000-0001-9599-0884</orcidid><orcidid>https://orcid.org/0000-0003-0690-7503</orcidid><orcidid>https://orcid.org/0000-0003-2056-3065</orcidid></search><sort><creationdate>20210522</creationdate><title>Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering</title><author>Lin, Chih-Hsun ; Hsia, Kai ; Su, Chih-Kuan ; Chen, Chien-Chin ; Yeh, Chang-Ching ; Ma, Hsu ; Lu, Jen-Her</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-4d91680289aabb5ee864ec6068aa9c9a707369a850881c90717d4590d17ab2613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Angiography</topic><topic>Biocompatibility</topic><topic>Biomechanics</topic><topic>Cavitation</topic><topic>Collagen</topic><topic>Coronary vessels</topic><topic>Endothelial cells</topic><topic>Extracellular matrix</topic><topic>Grafting</topic><topic>Histology</topic><topic>Hospitals</topic><topic>Laboratory animals</topic><topic>Life sciences</topic><topic>Magnetic resonance</topic><topic>Mechanical properties</topic><topic>Tissue engineering</topic><topic>Ultrasonic testing</topic><topic>Vascular tissue</topic><topic>Veins & arteries</topic><topic>Washing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Chih-Hsun</creatorcontrib><creatorcontrib>Hsia, Kai</creatorcontrib><creatorcontrib>Su, Chih-Kuan</creatorcontrib><creatorcontrib>Chen, Chien-Chin</creatorcontrib><creatorcontrib>Yeh, Chang-Ching</creatorcontrib><creatorcontrib>Ma, Hsu</creatorcontrib><creatorcontrib>Lu, Jen-Her</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Chih-Hsun</au><au>Hsia, Kai</au><au>Su, Chih-Kuan</au><au>Chen, Chien-Chin</au><au>Yeh, Chang-Ching</au><au>Ma, Hsu</au><au>Lu, Jen-Her</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering</atitle><jtitle>Polymers</jtitle><addtitle>Polymers (Basel)</addtitle><date>2021-05-22</date><risdate>2021</risdate><volume>13</volume><issue>11</issue><spage>1699</spage><pages>1699-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>Decellularized vascular grafts are useful for the construction of biological small-diameter tissue-engineered vascular grafts (≤6 mm). Traditional chemical decellularization requires a long treatment time, which may damage the structure and alter the mechanical properties. Decellularization using sonication is expected to solve this problem. The aim of this study was to develop an effective decellularization method using ultrasound followed by washing. Different power values of sonication at 40 kHz were tested for 2, 4, and 8 h followed by a washing procedure. The efficacy of sonication of decellularized human umbilical artery (sDHUA) was evaluated via DNA content, histological staining, mechanical properties, and biocompatibility. The sDHUAs were further implanted into rats for up to 90 days and magnetic resonance angiography (MRA) was performed for the implanted grafts. The results demonstrated that treatment of human umbilical artery (HUA) by sonication at ultrasonic power of 204 W for 4 h followed by washing for 24 h in 2% SDS buffer could eliminate more than 90% of cells and retain similar mechanical properties of the HUA. Recellularization was assessed by scanning electron microscopy (SEM), which indicated that sDHUA provided niches for human umbilical vein endothelial cells (HUVECs) to reside, indicating in vitro cytocompatibility. Further implantation tests also indicated the fitness of the sonication-treated HUA as a scaffold for small-caliber tissue engineering vascular grafts.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>34067495</pmid><doi>10.3390/polym13111699</doi><orcidid>https://orcid.org/0000-0002-9771-5816</orcidid><orcidid>https://orcid.org/0000-0001-9599-0884</orcidid><orcidid>https://orcid.org/0000-0003-0690-7503</orcidid><orcidid>https://orcid.org/0000-0003-2056-3065</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Angiography Biocompatibility Biomechanics Cavitation Collagen Coronary vessels Endothelial cells Extracellular matrix Grafting Histology Hospitals Laboratory animals Life sciences Magnetic resonance Mechanical properties Tissue engineering Ultrasonic testing Vascular tissue Veins & arteries Washing |
| title | Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering |
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