Amplicon-sequencing of raw milk microbiota: impact of DNA extraction and library-PCR

The highly complex raw milk matrix challenges the sample preparation for amplicon-sequencing due to low bacterial counts and high amounts of eukaryotic DNA originating from the cow. In this study, we optimized the extraction of bacterial DNA from raw milk for microbiome analysis and evaluated the im...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Applied microbiology and biotechnology 2021-06, Vol.105 (11), p.4761-4773
Hauptverfasser:	Siebert, Annemarie, Hofmann, Katharina, Staib, Lena, Doll, Etienne V., Scherer, Siegfried, Wenning, Mareike
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetic acid Bacteria Biodiversity Biomedical and Life Sciences Biotechnology Casein Deoxyribonucleic acid Digestion DNA DNA sequencing Endopeptidase K Ethylenediaminetetraacetic acids Gene sequencing Genetic aspects Libraries Life Sciences Lysis Methods Methods and Protocols Micelles Microbial Genetics and Genomics Microbiology Microbiomes Microbiota Microbiota (Symbiotic organisms) Microorganisms Milk Nucleotide sequencing Physiological aspects Polymerase chain reaction Proteinase Reduction rRNA 16S Sample preparation Somatic cells Species diversity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!