Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteins

Genome engineering of primary human cells with CRISPR-Cas9 has revolutionized experimental and therapeutic approaches to cell biology, but human myeloid-lineage cells have remained largely genetically intractable. We present a method for the delivery of CRISPR-Cas9 ribonucleoprotein (RNP) complexes...

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Veröffentlicht in:	Cell reports (Cambridge) 2021-05, Vol.35 (6), p.109105-109105, Article 109105
Hauptverfasser:	Hiatt, Joseph, Cavero, Devin A., McGregor, Michael J., Zheng, Weihao, Budzik, Jonathan M., Roth, Theodore L., Haas, Kelsey M., Wu, David, Rathore, Ujjwal, Meyer-Franke, Anke, Bouzidi, Mohamed S., Shifrut, Eric, Lee, Youjin, Kumar, Vigneshwari Easwar, Dang, Eric V., Gordon, David E., Wojcechowskyj, Jason A., Hultquist, Judd F., Fontaine, Krystal A., Pillai, Satish K., Cox, Jeffery S., Ernst, Joel D., Krogan, Nevan J., Marson, Alexander
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cas9 CRISPR CRISPR-Cas Systems - genetics dendritic cells electroporation Genome - genetics host-pathogen interactions Humans knockout macrophages Mice monocytes myeloid cells Myeloid Cells - metabolism ribonculeoproteins (RNPs) Ribonucleoproteins - metabolism
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