Protective effects of recombinant 53-kDa protein of Trichinella spiralis on acute lung injury in mice via alleviating lung pyroptosis by promoting M2 macrophage polarization
Trichinella spiralis represents an effective treatment for autoimmune and inflammatory diseases. The effects of recombinant T. spiralis (TS) 53-kDa protein (rTsP53) on acute lung injury (ALI) remain unclear. Here, mice were divided randomly into a control group, LPS group, and rTsP53 + LPS group. AL...
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| Veröffentlicht in: | Innate immunity (London, England) England), 2021-05, Vol.27 (4), p.313-323 |
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| creator | Wei, Ling-yu Jiang, An-qi Jiang, Ren Duan, Si-ying Xu, Xue Su, Ze-da-zhong Xu, Jia |
| description | Trichinella spiralis represents an effective treatment for autoimmune and inflammatory diseases. The effects of recombinant T. spiralis (TS) 53-kDa protein (rTsP53) on acute lung injury (ALI) remain unclear. Here, mice were divided randomly into a control group, LPS group, and rTsP53 + LPS group. ALI was induced in BALB/c mice by LPS (10 mg/kg) injected via the tail vein. rTsP53 (200 µl; 0.4 μg/μl) was injected subcutaneously three times at an interval of 5 d before inducing ALI in the rTsP53+LPS group. Lung pathological score, the ratio and markers of classic activated macrophages (M1) and alternatively activated macrophages (M2), cytokine profiles in alveolar lavage fluid, and pyroptosis protein expression in lung tissue were investigated. RTsP53 decreased lung pathological score. Furthermore, rTsP53 suppressed inflammation by increasing IL-4, IL-10, and IL-13. There was an increase in alveolar M2 macrophage numbers, with an increase in CD206 and arginase-1-positive cells and a decrease in alveolar M1 markers such as CD197 and iNOS. In addition, the polarization of M2 macrophages induced by rTsP53 treatment could alleviate ALI by suppressing lung pyroptosis. RTsP53 was identified as a potential agent for treating LPS-induced ALI via alleviating lung pyroptosis by promoting M2 macrophage polarization. |
| doi_str_mv | 10.1177/17534259211013397 |
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The effects of recombinant T. spiralis (TS) 53-kDa protein (rTsP53) on acute lung injury (ALI) remain unclear. Here, mice were divided randomly into a control group, LPS group, and rTsP53 + LPS group. ALI was induced in BALB/c mice by LPS (10 mg/kg) injected via the tail vein. rTsP53 (200 µl; 0.4 μg/μl) was injected subcutaneously three times at an interval of 5 d before inducing ALI in the rTsP53+LPS group. Lung pathological score, the ratio and markers of classic activated macrophages (M1) and alternatively activated macrophages (M2), cytokine profiles in alveolar lavage fluid, and pyroptosis protein expression in lung tissue were investigated. RTsP53 decreased lung pathological score. Furthermore, rTsP53 suppressed inflammation by increasing IL-4, IL-10, and IL-13. There was an increase in alveolar M2 macrophage numbers, with an increase in CD206 and arginase-1-positive cells and a decrease in alveolar M1 markers such as CD197 and iNOS. In addition, the polarization of M2 macrophages induced by rTsP53 treatment could alleviate ALI by suppressing lung pyroptosis. RTsP53 was identified as a potential agent for treating LPS-induced ALI via alleviating lung pyroptosis by promoting M2 macrophage polarization.</description><identifier>ISSN: 1753-4259</identifier><identifier>EISSN: 1753-4267</identifier><identifier>DOI: 10.1177/17534259211013397</identifier><identifier>PMID: 34013820</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Acute Lung Injury - chemically induced ; Acute Lung Injury - drug therapy ; Acute Lung Injury - pathology ; Alveoli ; Animals ; Arginase ; Bronchoalveolar Lavage Fluid ; Cell Count ; Cell Polarity - drug effects ; Cytokines - chemistry ; Inflammatory diseases ; Interleukin 10 ; Interleukin 13 ; Interleukin 4 ; Interleukins - antagonists & inhibitors ; Lipopolysaccharides ; Lungs ; Macrophage Activation - drug effects ; Macrophages ; Macrophages - drug effects ; Male ; Mannose Receptor - metabolism ; Mice ; Mice, Inbred BALB C ; Nitric Oxide Synthase Type II - metabolism ; Nitric-oxide synthase ; Original ; Polarization ; Protective Agents - pharmacology ; Proteins ; Pyroptosis ; Pyroptosis - drug effects ; Recombinant Proteins - pharmacology ; Trichinella spiralis ; Trichinella spiralis - chemistry</subject><ispartof>Innate immunity (London, England), 2021-05, Vol.27 (4), p.313-323</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. 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Manuscript content on this site is licensed under Creative Commons Licenses</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-8bb98ea2b79e575ea0e4d985c79c124ca20f3d953545877f0c9f945704a4fa793</citedby><cites>FETCH-LOGICAL-c532t-8bb98ea2b79e575ea0e4d985c79c124ca20f3d953545877f0c9f945704a4fa793</cites><orcidid>0000-0001-9869-1820</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186157/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186157/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,21945,27830,27901,27902,44921,45309,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34013820$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei, Ling-yu</creatorcontrib><creatorcontrib>Jiang, An-qi</creatorcontrib><creatorcontrib>Jiang, Ren</creatorcontrib><creatorcontrib>Duan, Si-ying</creatorcontrib><creatorcontrib>Xu, Xue</creatorcontrib><creatorcontrib>Su, Ze-da-zhong</creatorcontrib><creatorcontrib>Xu, Jia</creatorcontrib><title>Protective effects of recombinant 53-kDa protein of Trichinella spiralis on acute lung injury in mice via alleviating lung pyroptosis by promoting M2 macrophage polarization</title><title>Innate immunity (London, England)</title><addtitle>Innate Immun</addtitle><description>Trichinella spiralis represents an effective treatment for autoimmune and inflammatory diseases. The effects of recombinant T. spiralis (TS) 53-kDa protein (rTsP53) on acute lung injury (ALI) remain unclear. Here, mice were divided randomly into a control group, LPS group, and rTsP53 + LPS group. ALI was induced in BALB/c mice by LPS (10 mg/kg) injected via the tail vein. rTsP53 (200 µl; 0.4 μg/μl) was injected subcutaneously three times at an interval of 5 d before inducing ALI in the rTsP53+LPS group. Lung pathological score, the ratio and markers of classic activated macrophages (M1) and alternatively activated macrophages (M2), cytokine profiles in alveolar lavage fluid, and pyroptosis protein expression in lung tissue were investigated. RTsP53 decreased lung pathological score. Furthermore, rTsP53 suppressed inflammation by increasing IL-4, IL-10, and IL-13. There was an increase in alveolar M2 macrophage numbers, with an increase in CD206 and arginase-1-positive cells and a decrease in alveolar M1 markers such as CD197 and iNOS. 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The effects of recombinant T. spiralis (TS) 53-kDa protein (rTsP53) on acute lung injury (ALI) remain unclear. Here, mice were divided randomly into a control group, LPS group, and rTsP53 + LPS group. ALI was induced in BALB/c mice by LPS (10 mg/kg) injected via the tail vein. rTsP53 (200 µl; 0.4 μg/μl) was injected subcutaneously three times at an interval of 5 d before inducing ALI in the rTsP53+LPS group. Lung pathological score, the ratio and markers of classic activated macrophages (M1) and alternatively activated macrophages (M2), cytokine profiles in alveolar lavage fluid, and pyroptosis protein expression in lung tissue were investigated. RTsP53 decreased lung pathological score. Furthermore, rTsP53 suppressed inflammation by increasing IL-4, IL-10, and IL-13. There was an increase in alveolar M2 macrophage numbers, with an increase in CD206 and arginase-1-positive cells and a decrease in alveolar M1 markers such as CD197 and iNOS. In addition, the polarization of M2 macrophages induced by rTsP53 treatment could alleviate ALI by suppressing lung pyroptosis. RTsP53 was identified as a potential agent for treating LPS-induced ALI via alleviating lung pyroptosis by promoting M2 macrophage polarization.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>34013820</pmid><doi>10.1177/17534259211013397</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-9869-1820</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Sage Journals GOLD Open Access 2024; PubMed Central; Alma/SFX Local Collection |
| subjects | Acute Lung Injury - chemically induced Acute Lung Injury - drug therapy Acute Lung Injury - pathology Alveoli Animals Arginase Bronchoalveolar Lavage Fluid Cell Count Cell Polarity - drug effects Cytokines - chemistry Inflammatory diseases Interleukin 10 Interleukin 13 Interleukin 4 Interleukins - antagonists & inhibitors Lipopolysaccharides Lungs Macrophage Activation - drug effects Macrophages Macrophages - drug effects Male Mannose Receptor - metabolism Mice Mice, Inbred BALB C Nitric Oxide Synthase Type II - metabolism Nitric-oxide synthase Original Polarization Protective Agents - pharmacology Proteins Pyroptosis Pyroptosis - drug effects Recombinant Proteins - pharmacology Trichinella spiralis Trichinella spiralis - chemistry |
| title | Protective effects of recombinant 53-kDa protein of Trichinella spiralis on acute lung injury in mice via alleviating lung pyroptosis by promoting M2 macrophage polarization |
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