The immunogenic reaction and bone defect repair function of ε-poly-L-lysine (EPL)-coated nanoscale PCL/HA scaffold in rabbit calvarial bone defect
Tissue engineering is a promising strategy for bone tissue defect reconstruction. Immunogenic reaction, which was induced by scaffolds degradation or contaminating microorganism, influence cellular activity, compromise the efficiency of tissue engineering, or eventually lead to the failure of regene...
Gespeichert in:
Veröffentlicht in: | Journal of materials science. Materials in medicine 2021-06, Vol.32 (6), p.63-63, Article 63 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 63 |
---|---|
container_issue | 6 |
container_start_page | 63 |
container_title | Journal of materials science. Materials in medicine |
container_volume | 32 |
creator | Tian, Bin Wang, Na Jiang, Qingsong Tian, Lijiao Hu, Lei Zhang, Zhenting |
description | Tissue engineering is a promising strategy for bone tissue defect reconstruction. Immunogenic reaction, which was induced by scaffolds degradation or contaminating microorganism, influence cellular activity, compromise the efficiency of tissue engineering, or eventually lead to the failure of regeneration. Inhibiting excessive immune response through modulating scaffold is critical important to promote tissue regeneration. Our previous study showed that ε-poly-L-lysine (EPL)-coated nanoscale polycaprolactone/hydroxyapatite (EPL/PCL/HA) composite scaffold has enhanced antibacterial and osteogenic properties in vitro. However, the bone defect repair function and immunogenic reaction of EPL/PCL/HA scaffolds in vivo remains unclear. In the present study, three nanoscale scaffolds (EPL/PCL/HA, PCL and PCL/HA) were transplanted into rabbit paraspinal muscle pouches, and T helper type 1 (Th1), T helper type 2 (Th2), T helper type 17 (Th17), and macrophage infiltration were analyzed after 1 week and 2 weeks to detect their immunogenic reaction. Then, the different scaffolds were transplanted into rabbit calvarial bone defect to compare the bone defect repair capacities. The results showed that EPL/PCL/HA composite scaffolds decreased pro-inflammatory Th1, Th17, and type I macrophage infiltration from 1 to 2 weeks, and increased anti-inflammatory Th2 infiltration into the regenerated area at 2 weeks in vivo, when compared to PCL and PCL/HA. In addition, EPL/PCL/HA showed an enhanced bone repair capacity compared to PCL and PCL/HA when transplanted into rabbit calvarial bone defects at both 4 and 8 weeks. Hence, our results suggest that EPL could regulate the immunogenic reaction and promote bone defect repair function of PCL/HA, which is a promising agent for tissue engineering scaffold modulation. |
doi_str_mv | 10.1007/s10856-021-06533-7 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8184523</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2538052030</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-a60aedebb37a5b2984c0b2601d1a5898f74883ef50c8009007c633ac689c4203</originalsourceid><addsrcrecordid>eNp9kUFu1DAYhS0EokPhAiyQJTZlYfo7tmNng1SNCkWKRBeztxzHmbrK2IOdVJpzcBauwZnwkFIKC1aW9b7_Pf9-CL2m8J4CyPNMQYmaQEUJ1IIxIp-gFRWSEa6YeopW0AhJuGBwgl7kfAsAvBHiOTphHBpJOazQt82Nw363m0PcuuAtTs7YyceATehxF4PDvRucnYqwNz7hYQ6LHgf84zvZx_FAWjIesi_o2eV1-47YaCbX42BCzNaMDl-v2_OrC1wuwxDHHvuAk-k6P-Ei35nkzfg46iV6Npgxu1f35ynafLzcrK9I--XT5_VFSyyXfCKmBuN613VMGtFVjeIWuqoG2lMjVKMGyZVibhBgFUBTfszWjBlbq8byCtgp-rDY7udu53rrwpTMqPfJ70w66Gi8_lsJ_kZv451WVHFRsWJwdm-Q4tfZ5UnvfLZuHE1wcc66EkyBKEnHrLf_oLdxTqFsd6SkqksjR6paKJtizskND4-hoI-V66VyXSrXvyrXsgy9ebzGw8jvjgvAFiAXKWxd-pP9H9uf04-4Tw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2537863400</pqid></control><display><type>article</type><title>The immunogenic reaction and bone defect repair function of ε-poly-L-lysine (EPL)-coated nanoscale PCL/HA scaffold in rabbit calvarial bone defect</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Springer Nature OA Free Journals</source><creator>Tian, Bin ; Wang, Na ; Jiang, Qingsong ; Tian, Lijiao ; Hu, Lei ; Zhang, Zhenting</creator><creatorcontrib>Tian, Bin ; Wang, Na ; Jiang, Qingsong ; Tian, Lijiao ; Hu, Lei ; Zhang, Zhenting</creatorcontrib><description>Tissue engineering is a promising strategy for bone tissue defect reconstruction. Immunogenic reaction, which was induced by scaffolds degradation or contaminating microorganism, influence cellular activity, compromise the efficiency of tissue engineering, or eventually lead to the failure of regeneration. Inhibiting excessive immune response through modulating scaffold is critical important to promote tissue regeneration. Our previous study showed that ε-poly-L-lysine (EPL)-coated nanoscale polycaprolactone/hydroxyapatite (EPL/PCL/HA) composite scaffold has enhanced antibacterial and osteogenic properties in vitro. However, the bone defect repair function and immunogenic reaction of EPL/PCL/HA scaffolds in vivo remains unclear. In the present study, three nanoscale scaffolds (EPL/PCL/HA, PCL and PCL/HA) were transplanted into rabbit paraspinal muscle pouches, and T helper type 1 (Th1), T helper type 2 (Th2), T helper type 17 (Th17), and macrophage infiltration were analyzed after 1 week and 2 weeks to detect their immunogenic reaction. Then, the different scaffolds were transplanted into rabbit calvarial bone defect to compare the bone defect repair capacities. The results showed that EPL/PCL/HA composite scaffolds decreased pro-inflammatory Th1, Th17, and type I macrophage infiltration from 1 to 2 weeks, and increased anti-inflammatory Th2 infiltration into the regenerated area at 2 weeks in vivo, when compared to PCL and PCL/HA. In addition, EPL/PCL/HA showed an enhanced bone repair capacity compared to PCL and PCL/HA when transplanted into rabbit calvarial bone defects at both 4 and 8 weeks. Hence, our results suggest that EPL could regulate the immunogenic reaction and promote bone defect repair function of PCL/HA, which is a promising agent for tissue engineering scaffold modulation.</description><identifier>ISSN: 0957-4530</identifier><identifier>EISSN: 1573-4838</identifier><identifier>DOI: 10.1007/s10856-021-06533-7</identifier><identifier>PMID: 34097140</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Biodegradation ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biomedical materials ; Bone and Bones - metabolism ; Bone healing ; Bone Regeneration - drug effects ; Cell Adhesion ; Cell Proliferation ; Ceramics ; Chemistry and Materials Science ; Composites ; Durapatite - chemistry ; Durapatite - pharmacology ; Fractures, Bone - therapy ; Glass ; Helper cells ; Hydroxyapatite ; Immune response ; Immune system ; Immunogenicity ; Immunohistochemistry ; In vivo methods and tests ; Infiltration ; Inflammation ; Lymphocytes T ; Lysine ; Macrophages ; Macrophages - cytology ; Macrophages - metabolism ; Male ; Materials Science ; Muscles ; Natural Materials ; Osteogenesis - drug effects ; Paraspinal Muscles ; Poly-L-lysine ; Polycaprolactone ; Polyesters - chemistry ; Polyesters - pharmacology ; Polylysine - chemistry ; Polymer Sciences ; Rabbits ; Regeneration ; Regenerative Medicine/Tissue Engineering ; Repair ; Scaffolds ; Surfaces and Interfaces ; Th2 Cells ; Thin Films ; Tissue engineering ; Tissue Engineering - methods ; Tissue Engineering Constructs and Cell Substrates ; Tissue Scaffolds - chemistry ; Wound Healing - drug effects</subject><ispartof>Journal of materials science. Materials in medicine, 2021-06, Vol.32 (6), p.63-63, Article 63</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-a60aedebb37a5b2984c0b2601d1a5898f74883ef50c8009007c633ac689c4203</citedby><cites>FETCH-LOGICAL-c474t-a60aedebb37a5b2984c0b2601d1a5898f74883ef50c8009007c633ac689c4203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10856-021-06533-7$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://doi.org/10.1007/s10856-021-06533-7$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,860,881,27901,27902,41096,41464,42165,42533,51294,51551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34097140$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tian, Bin</creatorcontrib><creatorcontrib>Wang, Na</creatorcontrib><creatorcontrib>Jiang, Qingsong</creatorcontrib><creatorcontrib>Tian, Lijiao</creatorcontrib><creatorcontrib>Hu, Lei</creatorcontrib><creatorcontrib>Zhang, Zhenting</creatorcontrib><title>The immunogenic reaction and bone defect repair function of ε-poly-L-lysine (EPL)-coated nanoscale PCL/HA scaffold in rabbit calvarial bone defect</title><title>Journal of materials science. Materials in medicine</title><addtitle>J Mater Sci: Mater Med</addtitle><addtitle>J Mater Sci Mater Med</addtitle><description>Tissue engineering is a promising strategy for bone tissue defect reconstruction. Immunogenic reaction, which was induced by scaffolds degradation or contaminating microorganism, influence cellular activity, compromise the efficiency of tissue engineering, or eventually lead to the failure of regeneration. Inhibiting excessive immune response through modulating scaffold is critical important to promote tissue regeneration. Our previous study showed that ε-poly-L-lysine (EPL)-coated nanoscale polycaprolactone/hydroxyapatite (EPL/PCL/HA) composite scaffold has enhanced antibacterial and osteogenic properties in vitro. However, the bone defect repair function and immunogenic reaction of EPL/PCL/HA scaffolds in vivo remains unclear. In the present study, three nanoscale scaffolds (EPL/PCL/HA, PCL and PCL/HA) were transplanted into rabbit paraspinal muscle pouches, and T helper type 1 (Th1), T helper type 2 (Th2), T helper type 17 (Th17), and macrophage infiltration were analyzed after 1 week and 2 weeks to detect their immunogenic reaction. Then, the different scaffolds were transplanted into rabbit calvarial bone defect to compare the bone defect repair capacities. The results showed that EPL/PCL/HA composite scaffolds decreased pro-inflammatory Th1, Th17, and type I macrophage infiltration from 1 to 2 weeks, and increased anti-inflammatory Th2 infiltration into the regenerated area at 2 weeks in vivo, when compared to PCL and PCL/HA. In addition, EPL/PCL/HA showed an enhanced bone repair capacity compared to PCL and PCL/HA when transplanted into rabbit calvarial bone defects at both 4 and 8 weeks. Hence, our results suggest that EPL could regulate the immunogenic reaction and promote bone defect repair function of PCL/HA, which is a promising agent for tissue engineering scaffold modulation.</description><subject>Animals</subject><subject>Biodegradation</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical materials</subject><subject>Bone and Bones - metabolism</subject><subject>Bone healing</subject><subject>Bone Regeneration - drug effects</subject><subject>Cell Adhesion</subject><subject>Cell Proliferation</subject><subject>Ceramics</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>Durapatite - chemistry</subject><subject>Durapatite - pharmacology</subject><subject>Fractures, Bone - therapy</subject><subject>Glass</subject><subject>Helper cells</subject><subject>Hydroxyapatite</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunogenicity</subject><subject>Immunohistochemistry</subject><subject>In vivo methods and tests</subject><subject>Infiltration</subject><subject>Inflammation</subject><subject>Lymphocytes T</subject><subject>Lysine</subject><subject>Macrophages</subject><subject>Macrophages - cytology</subject><subject>Macrophages - metabolism</subject><subject>Male</subject><subject>Materials Science</subject><subject>Muscles</subject><subject>Natural Materials</subject><subject>Osteogenesis - drug effects</subject><subject>Paraspinal Muscles</subject><subject>Poly-L-lysine</subject><subject>Polycaprolactone</subject><subject>Polyesters - chemistry</subject><subject>Polyesters - pharmacology</subject><subject>Polylysine - chemistry</subject><subject>Polymer Sciences</subject><subject>Rabbits</subject><subject>Regeneration</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Repair</subject><subject>Scaffolds</subject><subject>Surfaces and Interfaces</subject><subject>Th2 Cells</subject><subject>Thin Films</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Engineering Constructs and Cell Substrates</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Wound Healing - drug effects</subject><issn>0957-4530</issn><issn>1573-4838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUFu1DAYhS0EokPhAiyQJTZlYfo7tmNng1SNCkWKRBeztxzHmbrK2IOdVJpzcBauwZnwkFIKC1aW9b7_Pf9-CL2m8J4CyPNMQYmaQEUJ1IIxIp-gFRWSEa6YeopW0AhJuGBwgl7kfAsAvBHiOTphHBpJOazQt82Nw363m0PcuuAtTs7YyceATehxF4PDvRucnYqwNz7hYQ6LHgf84zvZx_FAWjIesi_o2eV1-47YaCbX42BCzNaMDl-v2_OrC1wuwxDHHvuAk-k6P-Ei35nkzfg46iV6Npgxu1f35ynafLzcrK9I--XT5_VFSyyXfCKmBuN613VMGtFVjeIWuqoG2lMjVKMGyZVibhBgFUBTfszWjBlbq8byCtgp-rDY7udu53rrwpTMqPfJ70w66Gi8_lsJ_kZv451WVHFRsWJwdm-Q4tfZ5UnvfLZuHE1wcc66EkyBKEnHrLf_oLdxTqFsd6SkqksjR6paKJtizskND4-hoI-V66VyXSrXvyrXsgy9ebzGw8jvjgvAFiAXKWxd-pP9H9uf04-4Tw</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Tian, Bin</creator><creator>Wang, Na</creator><creator>Jiang, Qingsong</creator><creator>Tian, Lijiao</creator><creator>Hu, Lei</creator><creator>Zhang, Zhenting</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0W</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210601</creationdate><title>The immunogenic reaction and bone defect repair function of ε-poly-L-lysine (EPL)-coated nanoscale PCL/HA scaffold in rabbit calvarial bone defect</title><author>Tian, Bin ; Wang, Na ; Jiang, Qingsong ; Tian, Lijiao ; Hu, Lei ; Zhang, Zhenting</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-a60aedebb37a5b2984c0b2601d1a5898f74883ef50c8009007c633ac689c4203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Biodegradation</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedical materials</topic><topic>Bone and Bones - metabolism</topic><topic>Bone healing</topic><topic>Bone Regeneration - drug effects</topic><topic>Cell Adhesion</topic><topic>Cell Proliferation</topic><topic>Ceramics</topic><topic>Chemistry and Materials Science</topic><topic>Composites</topic><topic>Durapatite - chemistry</topic><topic>Durapatite - pharmacology</topic><topic>Fractures, Bone - therapy</topic><topic>Glass</topic><topic>Helper cells</topic><topic>Hydroxyapatite</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunogenicity</topic><topic>Immunohistochemistry</topic><topic>In vivo methods and tests</topic><topic>Infiltration</topic><topic>Inflammation</topic><topic>Lymphocytes T</topic><topic>Lysine</topic><topic>Macrophages</topic><topic>Macrophages - cytology</topic><topic>Macrophages - metabolism</topic><topic>Male</topic><topic>Materials Science</topic><topic>Muscles</topic><topic>Natural Materials</topic><topic>Osteogenesis - drug effects</topic><topic>Paraspinal Muscles</topic><topic>Poly-L-lysine</topic><topic>Polycaprolactone</topic><topic>Polyesters - chemistry</topic><topic>Polyesters - pharmacology</topic><topic>Polylysine - chemistry</topic><topic>Polymer Sciences</topic><topic>Rabbits</topic><topic>Regeneration</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Repair</topic><topic>Scaffolds</topic><topic>Surfaces and Interfaces</topic><topic>Th2 Cells</topic><topic>Thin Films</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Engineering Constructs and Cell Substrates</topic><topic>Tissue Scaffolds - chemistry</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tian, Bin</creatorcontrib><creatorcontrib>Wang, Na</creatorcontrib><creatorcontrib>Jiang, Qingsong</creatorcontrib><creatorcontrib>Tian, Lijiao</creatorcontrib><creatorcontrib>Hu, Lei</creatorcontrib><creatorcontrib>Zhang, Zhenting</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>DELNET Engineering & Technology Collection</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of materials science. Materials in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tian, Bin</au><au>Wang, Na</au><au>Jiang, Qingsong</au><au>Tian, Lijiao</au><au>Hu, Lei</au><au>Zhang, Zhenting</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The immunogenic reaction and bone defect repair function of ε-poly-L-lysine (EPL)-coated nanoscale PCL/HA scaffold in rabbit calvarial bone defect</atitle><jtitle>Journal of materials science. Materials in medicine</jtitle><stitle>J Mater Sci: Mater Med</stitle><addtitle>J Mater Sci Mater Med</addtitle><date>2021-06-01</date><risdate>2021</risdate><volume>32</volume><issue>6</issue><spage>63</spage><epage>63</epage><pages>63-63</pages><artnum>63</artnum><issn>0957-4530</issn><eissn>1573-4838</eissn><abstract>Tissue engineering is a promising strategy for bone tissue defect reconstruction. Immunogenic reaction, which was induced by scaffolds degradation or contaminating microorganism, influence cellular activity, compromise the efficiency of tissue engineering, or eventually lead to the failure of regeneration. Inhibiting excessive immune response through modulating scaffold is critical important to promote tissue regeneration. Our previous study showed that ε-poly-L-lysine (EPL)-coated nanoscale polycaprolactone/hydroxyapatite (EPL/PCL/HA) composite scaffold has enhanced antibacterial and osteogenic properties in vitro. However, the bone defect repair function and immunogenic reaction of EPL/PCL/HA scaffolds in vivo remains unclear. In the present study, three nanoscale scaffolds (EPL/PCL/HA, PCL and PCL/HA) were transplanted into rabbit paraspinal muscle pouches, and T helper type 1 (Th1), T helper type 2 (Th2), T helper type 17 (Th17), and macrophage infiltration were analyzed after 1 week and 2 weeks to detect their immunogenic reaction. Then, the different scaffolds were transplanted into rabbit calvarial bone defect to compare the bone defect repair capacities. The results showed that EPL/PCL/HA composite scaffolds decreased pro-inflammatory Th1, Th17, and type I macrophage infiltration from 1 to 2 weeks, and increased anti-inflammatory Th2 infiltration into the regenerated area at 2 weeks in vivo, when compared to PCL and PCL/HA. In addition, EPL/PCL/HA showed an enhanced bone repair capacity compared to PCL and PCL/HA when transplanted into rabbit calvarial bone defects at both 4 and 8 weeks. Hence, our results suggest that EPL could regulate the immunogenic reaction and promote bone defect repair function of PCL/HA, which is a promising agent for tissue engineering scaffold modulation.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>34097140</pmid><doi>10.1007/s10856-021-06533-7</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0957-4530 |
ispartof | Journal of materials science. Materials in medicine, 2021-06, Vol.32 (6), p.63-63, Article 63 |
issn | 0957-4530 1573-4838 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8184523 |
source | MEDLINE; Springer Nature - Complete Springer Journals; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Springer Nature OA Free Journals |
subjects | Animals Biodegradation Biomaterials Biomedical Engineering and Bioengineering Biomedical materials Bone and Bones - metabolism Bone healing Bone Regeneration - drug effects Cell Adhesion Cell Proliferation Ceramics Chemistry and Materials Science Composites Durapatite - chemistry Durapatite - pharmacology Fractures, Bone - therapy Glass Helper cells Hydroxyapatite Immune response Immune system Immunogenicity Immunohistochemistry In vivo methods and tests Infiltration Inflammation Lymphocytes T Lysine Macrophages Macrophages - cytology Macrophages - metabolism Male Materials Science Muscles Natural Materials Osteogenesis - drug effects Paraspinal Muscles Poly-L-lysine Polycaprolactone Polyesters - chemistry Polyesters - pharmacology Polylysine - chemistry Polymer Sciences Rabbits Regeneration Regenerative Medicine/Tissue Engineering Repair Scaffolds Surfaces and Interfaces Th2 Cells Thin Films Tissue engineering Tissue Engineering - methods Tissue Engineering Constructs and Cell Substrates Tissue Scaffolds - chemistry Wound Healing - drug effects |
title | The immunogenic reaction and bone defect repair function of ε-poly-L-lysine (EPL)-coated nanoscale PCL/HA scaffold in rabbit calvarial bone defect |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T21%3A16%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20immunogenic%20reaction%20and%20bone%20defect%20repair%20function%20of%20%CE%B5-poly-L-lysine%20(EPL)-coated%20nanoscale%20PCL/HA%20scaffold%20in%20rabbit%20calvarial%20bone%20defect&rft.jtitle=Journal%20of%20materials%20science.%20Materials%20in%20medicine&rft.au=Tian,%20Bin&rft.date=2021-06-01&rft.volume=32&rft.issue=6&rft.spage=63&rft.epage=63&rft.pages=63-63&rft.artnum=63&rft.issn=0957-4530&rft.eissn=1573-4838&rft_id=info:doi/10.1007/s10856-021-06533-7&rft_dat=%3Cproquest_pubme%3E2538052030%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2537863400&rft_id=info:pmid/34097140&rfr_iscdi=true |