Poly(ethylene-imine)-Functionalized Magnetite Nanoparticles Derivatized with Folic Acid: Heating and Targeting Properties
Magnetite nanoparticles (MNPs) coated by branched poly (ethylene-imine) (PEI) were synthesized in a one-pot. Three molecular weights of PEI were tested, namely, 1.8 kDa (sample MNP-1), 10 kDa (sample MNP-2), and 25 kDa (sample MNP-3). The MNP-1 particles were further functionalized with folic acid (...
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| Veröffentlicht in: | Polymers 2021-05, Vol.13 (10), p.1599 |
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| creator | Ortega-Muñoz, Mariano Plesselova, Simona Delgado, Angel V. Santoyo-Gonzalez, Francisco Salto-Gonzalez, Rafael Giron-Gonzalez, Maria Dolores Iglesias, Guillermo R. López-Jaramillo, Francisco Javier |
| description | Magnetite nanoparticles (MNPs) coated by branched poly (ethylene-imine) (PEI) were synthesized in a one-pot. Three molecular weights of PEI were tested, namely, 1.8 kDa (sample MNP-1), 10 kDa (sample MNP-2), and 25 kDa (sample MNP-3). The MNP-1 particles were further functionalized with folic acid (FA) (sample MNP-4). The four types of particles were found to behave magnetically as superparamagnetic, with MNP-1 showing the highest magnetization saturation. The particles were evaluated as possible hyperthermia agents by subjecting them to magnetic fields of 12 kA/m strength and frequencies ranging between 115 and 175 kHz. MNP-1 released the maximum heating power, reaching 330 W/g at the highest frequency, in the high side of reported values for spherical MNPs. In vitro cell viability assays of MNP-1 and MNP-4 against three cell lines expressing different levels of FA receptors (FR), namely, HEK (low expression), and HeLa (high expression), and HepG2 (high expression), demonstrated that they are not cytotoxic. When the cells were incubated in the presence of a 175 kHz magnetic field, a significant reduction in cell viability and clone formation was obtained for the high expressing FR cells incubated with MNP-4, suggesting that MNP-4 particles are good candidates for magnetic field hyperthermia and active targeting. |
| doi_str_mv | 10.3390/polym13101599 |
| format | Article |
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Three molecular weights of PEI were tested, namely, 1.8 kDa (sample MNP-1), 10 kDa (sample MNP-2), and 25 kDa (sample MNP-3). The MNP-1 particles were further functionalized with folic acid (FA) (sample MNP-4). The four types of particles were found to behave magnetically as superparamagnetic, with MNP-1 showing the highest magnetization saturation. The particles were evaluated as possible hyperthermia agents by subjecting them to magnetic fields of 12 kA/m strength and frequencies ranging between 115 and 175 kHz. MNP-1 released the maximum heating power, reaching 330 W/g at the highest frequency, in the high side of reported values for spherical MNPs. In vitro cell viability assays of MNP-1 and MNP-4 against three cell lines expressing different levels of FA receptors (FR), namely, HEK (low expression), and HeLa (high expression), and HepG2 (high expression), demonstrated that they are not cytotoxic. When the cells were incubated in the presence of a 175 kHz magnetic field, a significant reduction in cell viability and clone formation was obtained for the high expressing FR cells incubated with MNP-4, suggesting that MNP-4 particles are good candidates for magnetic field hyperthermia and active targeting.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym13101599</identifier><identifier>PMID: 34063481</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Acids ; Cancer therapies ; Chemical synthesis ; Ethylene ; Fever ; Folic acid ; Heating ; Hyperthermia ; Magnetic fields ; Magnetic properties ; Magnetite ; Nanoparticles ; Polymers ; Radiation ; Vitamin B</subject><ispartof>Polymers, 2021-05, Vol.13 (10), p.1599</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-cb1617f839cd2512fdf1792c76e672d463cec85624871e3d8c58e562548411493</citedby><cites>FETCH-LOGICAL-c392t-cb1617f839cd2512fdf1792c76e672d463cec85624871e3d8c58e562548411493</cites><orcidid>0000-0003-1843-5750 ; 0000-0002-5623-1516 ; 0000-0001-9638-988X ; 0000-0002-6030-7384 ; 0000-0002-7044-3611 ; 0000-0002-2142-3067</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155902/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155902/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Ortega-Muñoz, Mariano</creatorcontrib><creatorcontrib>Plesselova, Simona</creatorcontrib><creatorcontrib>Delgado, Angel V.</creatorcontrib><creatorcontrib>Santoyo-Gonzalez, Francisco</creatorcontrib><creatorcontrib>Salto-Gonzalez, Rafael</creatorcontrib><creatorcontrib>Giron-Gonzalez, Maria Dolores</creatorcontrib><creatorcontrib>Iglesias, Guillermo R.</creatorcontrib><creatorcontrib>López-Jaramillo, Francisco Javier</creatorcontrib><title>Poly(ethylene-imine)-Functionalized Magnetite Nanoparticles Derivatized with Folic Acid: Heating and Targeting Properties</title><title>Polymers</title><description>Magnetite nanoparticles (MNPs) coated by branched poly (ethylene-imine) (PEI) were synthesized in a one-pot. Three molecular weights of PEI were tested, namely, 1.8 kDa (sample MNP-1), 10 kDa (sample MNP-2), and 25 kDa (sample MNP-3). The MNP-1 particles were further functionalized with folic acid (FA) (sample MNP-4). The four types of particles were found to behave magnetically as superparamagnetic, with MNP-1 showing the highest magnetization saturation. The particles were evaluated as possible hyperthermia agents by subjecting them to magnetic fields of 12 kA/m strength and frequencies ranging between 115 and 175 kHz. MNP-1 released the maximum heating power, reaching 330 W/g at the highest frequency, in the high side of reported values for spherical MNPs. In vitro cell viability assays of MNP-1 and MNP-4 against three cell lines expressing different levels of FA receptors (FR), namely, HEK (low expression), and HeLa (high expression), and HepG2 (high expression), demonstrated that they are not cytotoxic. When the cells were incubated in the presence of a 175 kHz magnetic field, a significant reduction in cell viability and clone formation was obtained for the high expressing FR cells incubated with MNP-4, suggesting that MNP-4 particles are good candidates for magnetic field hyperthermia and active targeting.</description><subject>Acids</subject><subject>Cancer therapies</subject><subject>Chemical synthesis</subject><subject>Ethylene</subject><subject>Fever</subject><subject>Folic acid</subject><subject>Heating</subject><subject>Hyperthermia</subject><subject>Magnetic fields</subject><subject>Magnetic properties</subject><subject>Magnetite</subject><subject>Nanoparticles</subject><subject>Polymers</subject><subject>Radiation</subject><subject>Vitamin B</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkc9PFTEQxxujEQIcvW_iBQ-r_bVt14MJAZ-YIHDAc1O6s--VdNu17WKef73lQQw4l5nJfOY7kxmE3hH8kbEef5qj306EEUy6vn-F9imWrOVM4NfP4j10lPMdrsY7IYh8i_YYx4JxRfbR9rpKHEPZbD0EaN3kAnxoV0uwxcVgvPsDQ_PDrAMUV6C5NCHOJhVnPeTmDJK7N2XH_HZl06yid7Y5sW743JxDrYR1Y8LQ3Ji0hl12neIMtR_yIXozGp_h6MkfoJ-rrzen5-3F1bfvpycXrWU9La29JXXnUbHeDrQjdBxGIntqpQAh6cAFs2BVJyhXkgAblO0U1LTjihPCe3aAvjzqzsvtBIOFUJLxek5uMmmro3H6ZSW4jV7He61I1_WYVoHjJ4EUfy2Qi55ctuC9CRCXrGnHBJeUiodZ7_9D7-KS6hl3FFVCEokr1T5SNsWcE4z_liFYP_xVv_gr-wuwS5ZA</recordid><startdate>20210515</startdate><enddate>20210515</enddate><creator>Ortega-Muñoz, Mariano</creator><creator>Plesselova, Simona</creator><creator>Delgado, Angel V.</creator><creator>Santoyo-Gonzalez, Francisco</creator><creator>Salto-Gonzalez, Rafael</creator><creator>Giron-Gonzalez, Maria Dolores</creator><creator>Iglesias, Guillermo R.</creator><creator>López-Jaramillo, Francisco Javier</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1843-5750</orcidid><orcidid>https://orcid.org/0000-0002-5623-1516</orcidid><orcidid>https://orcid.org/0000-0001-9638-988X</orcidid><orcidid>https://orcid.org/0000-0002-6030-7384</orcidid><orcidid>https://orcid.org/0000-0002-7044-3611</orcidid><orcidid>https://orcid.org/0000-0002-2142-3067</orcidid></search><sort><creationdate>20210515</creationdate><title>Poly(ethylene-imine)-Functionalized Magnetite Nanoparticles Derivatized with Folic Acid: Heating and Targeting Properties</title><author>Ortega-Muñoz, Mariano ; Plesselova, Simona ; Delgado, Angel V. ; Santoyo-Gonzalez, Francisco ; Salto-Gonzalez, Rafael ; Giron-Gonzalez, Maria Dolores ; Iglesias, Guillermo R. ; López-Jaramillo, Francisco Javier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-cb1617f839cd2512fdf1792c76e672d463cec85624871e3d8c58e562548411493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acids</topic><topic>Cancer therapies</topic><topic>Chemical synthesis</topic><topic>Ethylene</topic><topic>Fever</topic><topic>Folic acid</topic><topic>Heating</topic><topic>Hyperthermia</topic><topic>Magnetic fields</topic><topic>Magnetic properties</topic><topic>Magnetite</topic><topic>Nanoparticles</topic><topic>Polymers</topic><topic>Radiation</topic><topic>Vitamin B</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ortega-Muñoz, Mariano</creatorcontrib><creatorcontrib>Plesselova, Simona</creatorcontrib><creatorcontrib>Delgado, Angel V.</creatorcontrib><creatorcontrib>Santoyo-Gonzalez, Francisco</creatorcontrib><creatorcontrib>Salto-Gonzalez, Rafael</creatorcontrib><creatorcontrib>Giron-Gonzalez, Maria Dolores</creatorcontrib><creatorcontrib>Iglesias, Guillermo R.</creatorcontrib><creatorcontrib>López-Jaramillo, Francisco Javier</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ortega-Muñoz, Mariano</au><au>Plesselova, Simona</au><au>Delgado, Angel V.</au><au>Santoyo-Gonzalez, Francisco</au><au>Salto-Gonzalez, Rafael</au><au>Giron-Gonzalez, Maria Dolores</au><au>Iglesias, Guillermo R.</au><au>López-Jaramillo, Francisco Javier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Poly(ethylene-imine)-Functionalized Magnetite Nanoparticles Derivatized with Folic Acid: Heating and Targeting Properties</atitle><jtitle>Polymers</jtitle><date>2021-05-15</date><risdate>2021</risdate><volume>13</volume><issue>10</issue><spage>1599</spage><pages>1599-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>Magnetite nanoparticles (MNPs) coated by branched poly (ethylene-imine) (PEI) were synthesized in a one-pot. Three molecular weights of PEI were tested, namely, 1.8 kDa (sample MNP-1), 10 kDa (sample MNP-2), and 25 kDa (sample MNP-3). The MNP-1 particles were further functionalized with folic acid (FA) (sample MNP-4). The four types of particles were found to behave magnetically as superparamagnetic, with MNP-1 showing the highest magnetization saturation. The particles were evaluated as possible hyperthermia agents by subjecting them to magnetic fields of 12 kA/m strength and frequencies ranging between 115 and 175 kHz. MNP-1 released the maximum heating power, reaching 330 W/g at the highest frequency, in the high side of reported values for spherical MNPs. In vitro cell viability assays of MNP-1 and MNP-4 against three cell lines expressing different levels of FA receptors (FR), namely, HEK (low expression), and HeLa (high expression), and HepG2 (high expression), demonstrated that they are not cytotoxic. When the cells were incubated in the presence of a 175 kHz magnetic field, a significant reduction in cell viability and clone formation was obtained for the high expressing FR cells incubated with MNP-4, suggesting that MNP-4 particles are good candidates for magnetic field hyperthermia and active targeting.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>34063481</pmid><doi>10.3390/polym13101599</doi><orcidid>https://orcid.org/0000-0003-1843-5750</orcidid><orcidid>https://orcid.org/0000-0002-5623-1516</orcidid><orcidid>https://orcid.org/0000-0001-9638-988X</orcidid><orcidid>https://orcid.org/0000-0002-6030-7384</orcidid><orcidid>https://orcid.org/0000-0002-7044-3611</orcidid><orcidid>https://orcid.org/0000-0002-2142-3067</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Cancer therapies Chemical synthesis Ethylene Fever Folic acid Heating Hyperthermia Magnetic fields Magnetic properties Magnetite Nanoparticles Polymers Radiation Vitamin B |
| title | Poly(ethylene-imine)-Functionalized Magnetite Nanoparticles Derivatized with Folic Acid: Heating and Targeting Properties |
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