Reversible RNA acylation for control of CRISPR–Cas9 gene editing

We report the development of post-transcriptional chemical methods that enable control over CRISPR–Cas9 gene editing activity both in in vitro assays and in living cells. We show that an azide-substituted acyl imidazole reagent (NAI-N 3 ) efficiently acylates CRISPR single guide RNAs (sgRNAs) in 20...

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Veröffentlicht in:	Chemical science (Cambridge) 2020, Vol.11 (4), p.1011-1016
Hauptverfasser:	Habibian, Maryam, McKinlay, Colin, Blake, Timothy R., Kietrys, Anna M., Waymouth, Robert M., Wender, Paul A., Kool, Eric T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acylation Carts Chemistry Cleavage Control methods CRISPR Deoxyribonucleic acid DNA Genetic modification Imidazole Organic chemistry Phosphines Reagents Ribonucleic acid RNA
Online-Zugang:	Volltext
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