Effects of propofol on the proliferation and migration of liver cancer cells
Liver cancer is a malignant cancer with worldwide prevalence. It has been reported that cancer cells are usually exposed to a hypoxic microenvironment, which is associated with a poor prognosis in patients with cancer. Propofol is an intravenous anesthetic that is widely used in cancer surgery. The...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2021-07, Vol.22 (1), p.733-733, Article 733 |
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| creator | Li, Chan Fu, Qingxia Cai, Jin Mei, Hongxia Shangguan, Wangning |
| description | Liver cancer is a malignant cancer with worldwide prevalence. It has been reported that cancer cells are usually exposed to a hypoxic microenvironment, which is associated with a poor prognosis in patients with cancer. Propofol is an intravenous anesthetic that is widely used in cancer surgery. The present study aimed to determine the effects of propofol stimulation on the viability, proliferation and migration of liver cancer cells under normoxia and cobalt chloride (Co[Cl.sub.2])-induced hypoxia. Under normoxia, HepG2 and HCCLM3 cells were randomly divided into six groups as follows: i) Control group; ii) 10 [micro]M propofol group; iii) 25 [micro]M propofol group; iv) 50 [micro]M propofol group; v) 100 [micro]M propofol group; and vi) DMSO group. Cell viability and proliferation were analyzed using Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays, respectively, following 24 or 48 h of propofol treatment. In addition, wound healing and Transwell migration assays were used to determine the changes in cell migration. Under Co[Cl.sub.2]-induced hypoxia, the protein levels of hypoxia inducible factor-1[alpha] (HIF-1[alpha]) of HepG2 cells were analyzed using western blotting. Subsequently, CCK-8 and wound healing assays were used to determine the effect of propofol on cell viability and migration. The results of the present study revealed that propofol stimulation had no significant effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxia. The protein levels of HIF-1[alpha] were significantly upregulated following the treatment with 200 [micro]M Co[Cl.sub.2] for 12 h. However, no significant differences were found in the viability and migration of HepG2 cells following the stimulation with propofol in the presence of Co[Cl.sub.2]. In conclusion, the findings of the present study revealed Keythat propofol exerted no effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxic and hypoxic conditions. Key words: propofol, liver cancer, viability, proliferation, migration |
| doi_str_mv | 10.3892/etm.2021.10165 |
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| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8138278</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A669991211</galeid><sourcerecordid>A669991211</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-3b30acdab1902abcbe71ca849744f51fcbde6e01025e979dce7c4b577a1c06ac3</originalsourceid><addsrcrecordid>eNptkk1LJDEQhoO4qLhz9dywFy8zppJOd3JZEPELBvaynkM6XRkj3cls0iP4703r4KKYHCpV9eQNFV5CzoCuuFTsAqdxxSiDFVBoxAE5gVaxZUnE4f5MlYRjssj5iZYlGpBSHJFjXlMhqKAnZH3tHNopV9FV2xS30cWhiqGaHnHOB-8wmcmXigl9NfrNPiv44J8xVdYEOwcchvyT_HBmyLjYx1PycHP99-puuf5ze391uV5arti05B2nxvamA0WZ6WyHLVgja9XWtRPgbNdjgxQoE6ha1Vtsbd2JtjVgaWMsPyW_33W3u27E0g9TMoPeJj-a9KKj8fpzJ_hHvYnPWgKXrJVF4HwvkOK_HeZJjz7PI5iAcZc1E1wAleWHCvrrC_oUdymU8WaK1YICr_9TGzOg9sHF8q6dRfVl0yilgAEUavUNVXaPo7cxoPOl_t0Fm2LOCd3HjED1bAFdLKBnC-g3C_BX_3eiIQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2532450134</pqid></control><display><type>article</type><title>Effects of propofol on the proliferation and migration of liver cancer cells</title><source>PubMed Central</source><creator>Li, Chan ; Fu, Qingxia ; Cai, Jin ; Mei, Hongxia ; Shangguan, Wangning</creator><creatorcontrib>Li, Chan ; Fu, Qingxia ; Cai, Jin ; Mei, Hongxia ; Shangguan, Wangning</creatorcontrib><description>Liver cancer is a malignant cancer with worldwide prevalence. It has been reported that cancer cells are usually exposed to a hypoxic microenvironment, which is associated with a poor prognosis in patients with cancer. Propofol is an intravenous anesthetic that is widely used in cancer surgery. The present study aimed to determine the effects of propofol stimulation on the viability, proliferation and migration of liver cancer cells under normoxia and cobalt chloride (Co[Cl.sub.2])-induced hypoxia. Under normoxia, HepG2 and HCCLM3 cells were randomly divided into six groups as follows: i) Control group; ii) 10 [micro]M propofol group; iii) 25 [micro]M propofol group; iv) 50 [micro]M propofol group; v) 100 [micro]M propofol group; and vi) DMSO group. Cell viability and proliferation were analyzed using Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays, respectively, following 24 or 48 h of propofol treatment. In addition, wound healing and Transwell migration assays were used to determine the changes in cell migration. Under Co[Cl.sub.2]-induced hypoxia, the protein levels of hypoxia inducible factor-1[alpha] (HIF-1[alpha]) of HepG2 cells were analyzed using western blotting. Subsequently, CCK-8 and wound healing assays were used to determine the effect of propofol on cell viability and migration. The results of the present study revealed that propofol stimulation had no significant effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxia. The protein levels of HIF-1[alpha] were significantly upregulated following the treatment with 200 [micro]M Co[Cl.sub.2] for 12 h. However, no significant differences were found in the viability and migration of HepG2 cells following the stimulation with propofol in the presence of Co[Cl.sub.2]. In conclusion, the findings of the present study revealed Keythat propofol exerted no effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxic and hypoxic conditions. Key words: propofol, liver cancer, viability, proliferation, migration</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2021.10165</identifier><identifier>PMID: 34055050</identifier><language>eng</language><publisher>Athens: Spandidos Publications</publisher><subject>Biotechnology ; Cancer cells ; Cell migration ; Cell proliferation ; Development and progression ; Hypoxia ; Investigations ; Liver cancer ; Medical prognosis ; Metastasis ; Pharmacology, Experimental ; Physiological aspects ; Propofol ; Proteins ; Surgery ; Tumors</subject><ispartof>Experimental and therapeutic medicine, 2021-07, Vol.22 (1), p.733-733, Article 733</ispartof><rights>COPYRIGHT 2021 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2021</rights><rights>Copyright: © Li et al. 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-3b30acdab1902abcbe71ca849744f51fcbde6e01025e979dce7c4b577a1c06ac3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138278/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138278/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Li, Chan</creatorcontrib><creatorcontrib>Fu, Qingxia</creatorcontrib><creatorcontrib>Cai, Jin</creatorcontrib><creatorcontrib>Mei, Hongxia</creatorcontrib><creatorcontrib>Shangguan, Wangning</creatorcontrib><title>Effects of propofol on the proliferation and migration of liver cancer cells</title><title>Experimental and therapeutic medicine</title><description>Liver cancer is a malignant cancer with worldwide prevalence. It has been reported that cancer cells are usually exposed to a hypoxic microenvironment, which is associated with a poor prognosis in patients with cancer. Propofol is an intravenous anesthetic that is widely used in cancer surgery. The present study aimed to determine the effects of propofol stimulation on the viability, proliferation and migration of liver cancer cells under normoxia and cobalt chloride (Co[Cl.sub.2])-induced hypoxia. Under normoxia, HepG2 and HCCLM3 cells were randomly divided into six groups as follows: i) Control group; ii) 10 [micro]M propofol group; iii) 25 [micro]M propofol group; iv) 50 [micro]M propofol group; v) 100 [micro]M propofol group; and vi) DMSO group. Cell viability and proliferation were analyzed using Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays, respectively, following 24 or 48 h of propofol treatment. In addition, wound healing and Transwell migration assays were used to determine the changes in cell migration. Under Co[Cl.sub.2]-induced hypoxia, the protein levels of hypoxia inducible factor-1[alpha] (HIF-1[alpha]) of HepG2 cells were analyzed using western blotting. Subsequently, CCK-8 and wound healing assays were used to determine the effect of propofol on cell viability and migration. The results of the present study revealed that propofol stimulation had no significant effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxia. The protein levels of HIF-1[alpha] were significantly upregulated following the treatment with 200 [micro]M Co[Cl.sub.2] for 12 h. However, no significant differences were found in the viability and migration of HepG2 cells following the stimulation with propofol in the presence of Co[Cl.sub.2]. In conclusion, the findings of the present study revealed Keythat propofol exerted no effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxic and hypoxic conditions. Key words: propofol, liver cancer, viability, proliferation, migration</description><subject>Biotechnology</subject><subject>Cancer cells</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Development and progression</subject><subject>Hypoxia</subject><subject>Investigations</subject><subject>Liver cancer</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>Pharmacology, Experimental</subject><subject>Physiological aspects</subject><subject>Propofol</subject><subject>Proteins</subject><subject>Surgery</subject><subject>Tumors</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkk1LJDEQhoO4qLhz9dywFy8zppJOd3JZEPELBvaynkM6XRkj3cls0iP4703r4KKYHCpV9eQNFV5CzoCuuFTsAqdxxSiDFVBoxAE5gVaxZUnE4f5MlYRjssj5iZYlGpBSHJFjXlMhqKAnZH3tHNopV9FV2xS30cWhiqGaHnHOB-8wmcmXigl9NfrNPiv44J8xVdYEOwcchvyT_HBmyLjYx1PycHP99-puuf5ze391uV5arti05B2nxvamA0WZ6WyHLVgja9XWtRPgbNdjgxQoE6ha1Vtsbd2JtjVgaWMsPyW_33W3u27E0g9TMoPeJj-a9KKj8fpzJ_hHvYnPWgKXrJVF4HwvkOK_HeZJjz7PI5iAcZc1E1wAleWHCvrrC_oUdymU8WaK1YICr_9TGzOg9sHF8q6dRfVl0yilgAEUavUNVXaPo7cxoPOl_t0Fm2LOCd3HjED1bAFdLKBnC-g3C_BX_3eiIQ</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Li, Chan</creator><creator>Fu, Qingxia</creator><creator>Cai, Jin</creator><creator>Mei, Hongxia</creator><creator>Shangguan, Wangning</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210701</creationdate><title>Effects of propofol on the proliferation and migration of liver cancer cells</title><author>Li, Chan ; Fu, Qingxia ; Cai, Jin ; Mei, Hongxia ; Shangguan, Wangning</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-3b30acdab1902abcbe71ca849744f51fcbde6e01025e979dce7c4b577a1c06ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biotechnology</topic><topic>Cancer cells</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Development and progression</topic><topic>Hypoxia</topic><topic>Investigations</topic><topic>Liver cancer</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>Pharmacology, Experimental</topic><topic>Physiological aspects</topic><topic>Propofol</topic><topic>Proteins</topic><topic>Surgery</topic><topic>Tumors</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Chan</creatorcontrib><creatorcontrib>Fu, Qingxia</creatorcontrib><creatorcontrib>Cai, Jin</creatorcontrib><creatorcontrib>Mei, Hongxia</creatorcontrib><creatorcontrib>Shangguan, Wangning</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Chan</au><au>Fu, Qingxia</au><au>Cai, Jin</au><au>Mei, Hongxia</au><au>Shangguan, Wangning</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of propofol on the proliferation and migration of liver cancer cells</atitle><jtitle>Experimental and therapeutic medicine</jtitle><date>2021-07-01</date><risdate>2021</risdate><volume>22</volume><issue>1</issue><spage>733</spage><epage>733</epage><pages>733-733</pages><artnum>733</artnum><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>Liver cancer is a malignant cancer with worldwide prevalence. It has been reported that cancer cells are usually exposed to a hypoxic microenvironment, which is associated with a poor prognosis in patients with cancer. Propofol is an intravenous anesthetic that is widely used in cancer surgery. The present study aimed to determine the effects of propofol stimulation on the viability, proliferation and migration of liver cancer cells under normoxia and cobalt chloride (Co[Cl.sub.2])-induced hypoxia. Under normoxia, HepG2 and HCCLM3 cells were randomly divided into six groups as follows: i) Control group; ii) 10 [micro]M propofol group; iii) 25 [micro]M propofol group; iv) 50 [micro]M propofol group; v) 100 [micro]M propofol group; and vi) DMSO group. Cell viability and proliferation were analyzed using Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays, respectively, following 24 or 48 h of propofol treatment. In addition, wound healing and Transwell migration assays were used to determine the changes in cell migration. Under Co[Cl.sub.2]-induced hypoxia, the protein levels of hypoxia inducible factor-1[alpha] (HIF-1[alpha]) of HepG2 cells were analyzed using western blotting. Subsequently, CCK-8 and wound healing assays were used to determine the effect of propofol on cell viability and migration. The results of the present study revealed that propofol stimulation had no significant effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxia. The protein levels of HIF-1[alpha] were significantly upregulated following the treatment with 200 [micro]M Co[Cl.sub.2] for 12 h. However, no significant differences were found in the viability and migration of HepG2 cells following the stimulation with propofol in the presence of Co[Cl.sub.2]. In conclusion, the findings of the present study revealed Keythat propofol exerted no effect on the viability, proliferation and migration of HepG2 and HCCLM3 cells under normoxic and hypoxic conditions. Key words: propofol, liver cancer, viability, proliferation, migration</abstract><cop>Athens</cop><pub>Spandidos Publications</pub><pmid>34055050</pmid><doi>10.3892/etm.2021.10165</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biotechnology Cancer cells Cell migration Cell proliferation Development and progression Hypoxia Investigations Liver cancer Medical prognosis Metastasis Pharmacology, Experimental Physiological aspects Propofol Proteins Surgery Tumors |
| title | Effects of propofol on the proliferation and migration of liver cancer cells |
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