Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River before and after a Municipal Wastewater Treatment Plant Replacement
Monitoring of the Colorado River near the Moab, Utah, wastewater treatment plant (WWTP) outflow has detected pharmaceuticals, hormones, and estrogen-receptor (ER)-, glucocorticoid receptor (GR)-, and peroxisome proliferator-activated receptor-gamma (PPARγ)-mediated biological activities. The aim of...
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| Veröffentlicht in: | Environmental science & technology 2021-01, Vol.55 (2), p.974-984 |
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| creator | Cavallin, Jenna E Battaglin, William A Beihoffer, Jon Blackwell, Brett R Bradley, Paul M Cole, Alexander R Ekman, Drew R Hofer, Rachel N Kinsey, Julie Keteles, Kristen Weissinger, Rebecca Winkelman, Dana L Villeneuve, Daniel L |
| description | Monitoring of the Colorado River near the Moab, Utah, wastewater treatment plant (WWTP) outflow has detected pharmaceuticals, hormones, and estrogen-receptor (ER)-, glucocorticoid receptor (GR)-, and peroxisome proliferator-activated receptor-gamma (PPARγ)-mediated biological activities. The aim of the present multi-year study was to assess effects of a WWTP replacement on bioactive chemical (BC) concentrations. Water samples were collected bimonthly, pre- and post-replacement, at 11 sites along the Colorado River upstream and downstream of the WWTP and analyzed for in vitro bioactivities (e.g., agonism of ER, GR, and PPARγ) and BC concentrations; fathead minnows were cage deployed pre- and post-replacement at sites with varying proximities to the WWTP. Before the WWTP replacement, in vitro ER (24 ng 17β-estradiol equivalents/L)-, GR (60 ng dexamethasone equivalents/L)-, and PPARγ-mediated activities were detected at the WWTP outflow but diminished downstream. In March 2018, the WWTP effluent was acutely toxic to the fish, likely due to elevated ammonia concentrations. Following the WWTP replacement, ER, GR, and PPARγ bioactivities were reduced by approximately 60–79%, no toxicity was observed in caged fish, and there were marked decreases in concentrations of many BCs. Results suggest that replacement of the Moab WWTP achieved a significant reduction in BC concentrations to the Colorado River. |
| doi_str_mv | 10.1021/acs.est.0c05269 |
| format | Article |
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The aim of the present multi-year study was to assess effects of a WWTP replacement on bioactive chemical (BC) concentrations. Water samples were collected bimonthly, pre- and post-replacement, at 11 sites along the Colorado River upstream and downstream of the WWTP and analyzed for in vitro bioactivities (e.g., agonism of ER, GR, and PPARγ) and BC concentrations; fathead minnows were cage deployed pre- and post-replacement at sites with varying proximities to the WWTP. Before the WWTP replacement, in vitro ER (24 ng 17β-estradiol equivalents/L)-, GR (60 ng dexamethasone equivalents/L)-, and PPARγ-mediated activities were detected at the WWTP outflow but diminished downstream. In March 2018, the WWTP effluent was acutely toxic to the fish, likely due to elevated ammonia concentrations. Following the WWTP replacement, ER, GR, and PPARγ bioactivities were reduced by approximately 60–79%, no toxicity was observed in caged fish, and there were marked decreases in concentrations of many BCs. Results suggest that replacement of the Moab WWTP achieved a significant reduction in BC concentrations to the Colorado River.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/acs.est.0c05269</identifier><identifier>PMID: 33373525</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>17β-Estradiol ; Acute toxicity ; Ammonia ; Animals ; Biochemistry ; Biocompatibility ; Biological activity ; Colorado ; Contaminants in Aquatic and Terrestrial Environments ; Dexamethasone ; Downstream ; Environmental Monitoring ; Equivalence ; Estrogen receptors ; Estrogens ; Fish ; Glucocorticoids ; Hormones ; Monitoring ; Municipal wastewater ; Outflow ; Receptors ; Rivers ; Sex hormones ; Toxicity ; Utah ; Waste Disposal, Fluid ; Waste Water ; Wastewater treatment ; Wastewater treatment plants ; Water analysis ; Water outflow ; Water Pollutants, Chemical - analysis ; Water Purification ; Water sampling ; Water treatment</subject><ispartof>Environmental science & technology, 2021-01, Vol.55 (2), p.974-984</ispartof><rights>2020 American Chemical Society</rights><rights>Copyright American Chemical Society Jan 19, 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a457t-f4b6491954e8fed3029ddb3c980f2d8608dfe9054d1b907ac761d9a852225ed03</citedby><cites>FETCH-LOGICAL-a457t-f4b6491954e8fed3029ddb3c980f2d8608dfe9054d1b907ac761d9a852225ed03</cites><orcidid>0000-0003-2801-0203 ; 0000-0001-7522-8606 ; 0000-0001-7883-4740 ; 0000-0003-1296-4539</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.est.0c05269$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.est.0c05269$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33373525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cavallin, Jenna E</creatorcontrib><creatorcontrib>Battaglin, William A</creatorcontrib><creatorcontrib>Beihoffer, Jon</creatorcontrib><creatorcontrib>Blackwell, Brett R</creatorcontrib><creatorcontrib>Bradley, Paul M</creatorcontrib><creatorcontrib>Cole, Alexander R</creatorcontrib><creatorcontrib>Ekman, Drew R</creatorcontrib><creatorcontrib>Hofer, Rachel N</creatorcontrib><creatorcontrib>Kinsey, Julie</creatorcontrib><creatorcontrib>Keteles, Kristen</creatorcontrib><creatorcontrib>Weissinger, Rebecca</creatorcontrib><creatorcontrib>Winkelman, Dana L</creatorcontrib><creatorcontrib>Villeneuve, Daniel L</creatorcontrib><title>Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River before and after a Municipal Wastewater Treatment Plant Replacement</title><title>Environmental science & technology</title><addtitle>Environ. 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Before the WWTP replacement, in vitro ER (24 ng 17β-estradiol equivalents/L)-, GR (60 ng dexamethasone equivalents/L)-, and PPARγ-mediated activities were detected at the WWTP outflow but diminished downstream. In March 2018, the WWTP effluent was acutely toxic to the fish, likely due to elevated ammonia concentrations. Following the WWTP replacement, ER, GR, and PPARγ bioactivities were reduced by approximately 60–79%, no toxicity was observed in caged fish, and there were marked decreases in concentrations of many BCs. Results suggest that replacement of the Moab WWTP achieved a significant reduction in BC concentrations to the Colorado River.</description><subject>17β-Estradiol</subject><subject>Acute toxicity</subject><subject>Ammonia</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biological activity</subject><subject>Colorado</subject><subject>Contaminants in Aquatic and Terrestrial Environments</subject><subject>Dexamethasone</subject><subject>Downstream</subject><subject>Environmental Monitoring</subject><subject>Equivalence</subject><subject>Estrogen receptors</subject><subject>Estrogens</subject><subject>Fish</subject><subject>Glucocorticoids</subject><subject>Hormones</subject><subject>Monitoring</subject><subject>Municipal wastewater</subject><subject>Outflow</subject><subject>Receptors</subject><subject>Rivers</subject><subject>Sex hormones</subject><subject>Toxicity</subject><subject>Utah</subject><subject>Waste Disposal, Fluid</subject><subject>Waste Water</subject><subject>Wastewater treatment</subject><subject>Wastewater treatment plants</subject><subject>Water analysis</subject><subject>Water outflow</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Purification</subject><subject>Water sampling</subject><subject>Water treatment</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtvEzEUhS0EomlhzQ5ZYokm9WM8sTdINJSH1ApUFcHOuuNH4moyDrZT1P_BD8ajhAgWbGzp3u-ec3UPQi8omVPC6DmYPHe5zIkhgnXqEZpRwUgjpKCP0YwQyhvFu-8n6DTnO0II40Q-RSec8wUXTMzQr0vvnSm5uYDsLL6OYygxhXGFo8cXIYIp4d7h5dptgoEh43chmzWkVYVLxGVde3GICWzEN5VMuHc-JodhtBh8qQXA17sxmLCFAX-DXNxPmMq3yUHZuLHgLwPU98ZtBzBuqjxDT3z1cs8P_xn6-v7ydvmxufr84dPy7VUDrViUxrd91yqqROukd5YTpqztuVGSeGZlR6T1ThHRWtorsgCz6KhVIAVjTDhL-Bl6s9fd7vqNs6ZaJxj0NoUNpAcdIeh_O2NY61W815LW6zFeBV4dBFL8satB6Lu4S2PdWbNWckJpJ0WlzveUSTHn5PzRgRI9xahrjHqaPsRYJ17-vdiR_5NbBV7vgWny6Pk_ud96YKvF</recordid><startdate>20210119</startdate><enddate>20210119</enddate><creator>Cavallin, Jenna E</creator><creator>Battaglin, William A</creator><creator>Beihoffer, Jon</creator><creator>Blackwell, Brett R</creator><creator>Bradley, Paul M</creator><creator>Cole, Alexander R</creator><creator>Ekman, Drew R</creator><creator>Hofer, Rachel N</creator><creator>Kinsey, Julie</creator><creator>Keteles, Kristen</creator><creator>Weissinger, Rebecca</creator><creator>Winkelman, Dana L</creator><creator>Villeneuve, Daniel L</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2801-0203</orcidid><orcidid>https://orcid.org/0000-0001-7522-8606</orcidid><orcidid>https://orcid.org/0000-0001-7883-4740</orcidid><orcidid>https://orcid.org/0000-0003-1296-4539</orcidid></search><sort><creationdate>20210119</creationdate><title>Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River before and after a Municipal Wastewater Treatment Plant Replacement</title><author>Cavallin, Jenna E ; 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Sci. Technol</addtitle><date>2021-01-19</date><risdate>2021</risdate><volume>55</volume><issue>2</issue><spage>974</spage><epage>984</epage><pages>974-984</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><abstract>Monitoring of the Colorado River near the Moab, Utah, wastewater treatment plant (WWTP) outflow has detected pharmaceuticals, hormones, and estrogen-receptor (ER)-, glucocorticoid receptor (GR)-, and peroxisome proliferator-activated receptor-gamma (PPARγ)-mediated biological activities. The aim of the present multi-year study was to assess effects of a WWTP replacement on bioactive chemical (BC) concentrations. Water samples were collected bimonthly, pre- and post-replacement, at 11 sites along the Colorado River upstream and downstream of the WWTP and analyzed for in vitro bioactivities (e.g., agonism of ER, GR, and PPARγ) and BC concentrations; fathead minnows were cage deployed pre- and post-replacement at sites with varying proximities to the WWTP. Before the WWTP replacement, in vitro ER (24 ng 17β-estradiol equivalents/L)-, GR (60 ng dexamethasone equivalents/L)-, and PPARγ-mediated activities were detected at the WWTP outflow but diminished downstream. In March 2018, the WWTP effluent was acutely toxic to the fish, likely due to elevated ammonia concentrations. Following the WWTP replacement, ER, GR, and PPARγ bioactivities were reduced by approximately 60–79%, no toxicity was observed in caged fish, and there were marked decreases in concentrations of many BCs. Results suggest that replacement of the Moab WWTP achieved a significant reduction in BC concentrations to the Colorado River.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>33373525</pmid><doi>10.1021/acs.est.0c05269</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-2801-0203</orcidid><orcidid>https://orcid.org/0000-0001-7522-8606</orcidid><orcidid>https://orcid.org/0000-0001-7883-4740</orcidid><orcidid>https://orcid.org/0000-0003-1296-4539</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; American Chemical Society (ACS) Journals |
| subjects | 17β-Estradiol Acute toxicity Ammonia Animals Biochemistry Biocompatibility Biological activity Colorado Contaminants in Aquatic and Terrestrial Environments Dexamethasone Downstream Environmental Monitoring Equivalence Estrogen receptors Estrogens Fish Glucocorticoids Hormones Monitoring Municipal wastewater Outflow Receptors Rivers Sex hormones Toxicity Utah Waste Disposal, Fluid Waste Water Wastewater treatment Wastewater treatment plants Water analysis Water outflow Water Pollutants, Chemical - analysis Water Purification Water sampling Water treatment |
| title | Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River before and after a Municipal Wastewater Treatment Plant Replacement |
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