3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells
3,4,5‑Trihydroxycinnamic acid (THCA) exhibits anti‑inflammatory activity in acute or chronic inflammatory disorders, such as acute lung injury and asthma. The present study investigated the anti‑inflammatory activity of THCA in a tumor necrosis factor‑α/interferon‑γ (TI) mixture‑stimulated human ker...
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| Veröffentlicht in: | Molecular medicine reports 2021-07, Vol.24 (1), Article 509 |
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| creator | Park, Ji-Won Oh, Jae-Hoon Hwang, Daseul Kim, Seong-Man Min, Jae-Hong Seo, Ji-Yun Chun, Wanjoo Lee, Hee Jae Oh, Sei-Ryang Lee, Jae-Won Ahn, Kyung-Seop |
| description | 3,4,5‑Trihydroxycinnamic acid (THCA) exhibits anti‑inflammatory activity in acute or chronic inflammatory disorders, such as acute lung injury and asthma. The present study investigated the anti‑inflammatory activity of THCA in a tumor necrosis factor‑α/interferon‑γ (TI) mixture‑stimulated human keratinocyte cell line. The results of ELISA and reverse transcription‑quantitative PCR revealed that THCA reduced the secretion and mRNA expression levels of interleukin (IL)‑6; IL‑8; thymus and activation‑regulated chemokine; macrophage‑derived chemokine; regulated upon activation, normal T cell expressed and secreted; and monocyte chemoattractant protein‑1 in TI mixture‑stimulated HaCaT cells. In addition, the results of western blot analysis demonstrated that THCA exerted inhibitory activity on the activation of AKT, ERK and nuclear factor‑κB in TI mixture‑stimulated HaCaT cells. Furthermore, THCA upregulated the expression levels of heme oxygenase‑1 and NAD(P)H:quinone oxidoreductase 1, and the activation of nuclear factor erythroid 2‑related factor 2 in HaCaT cells. These results demonstrated that THCA may exhibit anti‑inflammatory activity in activated HaCaT cells. |
| doi_str_mv | 10.3892/mmr.2021.12148 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8134876</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2536745802</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-8fd67fe140bf694a64d00b4ecf1a01d57491a1b532d00267575a7e23f90d90673</originalsourceid><addsrcrecordid>eNpdkc9OVDEYxRuDEUS3LM1N2Lhghv7v7YaETBwhIbgZ102nt4WS23Zo7yXMzlfwUYzv4UP4JPbKSNRN-6Xn15Pv5ABwhOCctBKfhpDnGGI0RxjR9gU4QEKiGYGQ7u1mLKXYB69LuYOQM8zkK7BPiGyx4PgAbMgJPWE_v3xdZX-77XJ63Bofow7eNNr4rrGPNg-l0XHwlfLR9ToEPaS8baxz1lQtxWZ1vazqj2-nl8vrafhejzL4MPZ6sF1zoRd61Rjb9-UNeOl0X-zb3X0IPi8_rBYXs6tPHy8X51czQ1E7zFrXceEsonDtuKSa0w7CNbXGIQ1RxwSVSKM1I7i-Yy6YYFpYTJyEnYRckENw9uS7GdfBdsbGIetebbIPOm9V0l79q0R_q27Sg2oRoa3g1eD9ziCn-9GWQQVfpgg62jQWhRnmBDIucUWP_0Pv0phjjVcpwgVlLZyo-RNlciolW_e8DIJqKlPVMtVUpvpdZv3w7u8Iz_if9sgvvQOhNg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2536745802</pqid></control><display><type>article</type><title>3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells</title><source>Spandidos Publications Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Park, Ji-Won ; Oh, Jae-Hoon ; Hwang, Daseul ; Kim, Seong-Man ; Min, Jae-Hong ; Seo, Ji-Yun ; Chun, Wanjoo ; Lee, Hee Jae ; Oh, Sei-Ryang ; Lee, Jae-Won ; Ahn, Kyung-Seop</creator><creatorcontrib>Park, Ji-Won ; Oh, Jae-Hoon ; Hwang, Daseul ; Kim, Seong-Man ; Min, Jae-Hong ; Seo, Ji-Yun ; Chun, Wanjoo ; Lee, Hee Jae ; Oh, Sei-Ryang ; Lee, Jae-Won ; Ahn, Kyung-Seop</creatorcontrib><description>3,4,5‑Trihydroxycinnamic acid (THCA) exhibits anti‑inflammatory activity in acute or chronic inflammatory disorders, such as acute lung injury and asthma. The present study investigated the anti‑inflammatory activity of THCA in a tumor necrosis factor‑α/interferon‑γ (TI) mixture‑stimulated human keratinocyte cell line. The results of ELISA and reverse transcription‑quantitative PCR revealed that THCA reduced the secretion and mRNA expression levels of interleukin (IL)‑6; IL‑8; thymus and activation‑regulated chemokine; macrophage‑derived chemokine; regulated upon activation, normal T cell expressed and secreted; and monocyte chemoattractant protein‑1 in TI mixture‑stimulated HaCaT cells. In addition, the results of western blot analysis demonstrated that THCA exerted inhibitory activity on the activation of AKT, ERK and nuclear factor‑κB in TI mixture‑stimulated HaCaT cells. Furthermore, THCA upregulated the expression levels of heme oxygenase‑1 and NAD(P)H:quinone oxidoreductase 1, and the activation of nuclear factor erythroid 2‑related factor 2 in HaCaT cells. These results demonstrated that THCA may exhibit anti‑inflammatory activity in activated HaCaT cells.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2021.12148</identifier><identifier>PMID: 33982762</identifier><language>eng</language><publisher>Greece: Spandidos Publications UK Ltd</publisher><subject>Acids ; AKT protein ; Anti-inflammatory agents ; Asthma ; CCL17 protein ; CCL22 protein ; Cell activation ; Cell culture ; Chemokines ; Cytokines ; Eczema ; Gene expression ; Heme ; Heme oxygenase (decyclizing) ; Inflammatory diseases ; Interleukin 8 ; Kinases ; Lymphocytes T ; Monocyte chemoattractant protein ; Monocyte chemoattractant protein 1 ; Monocytes ; NAD ; NADPH quinone oxidoreductase ; Oxygenase ; Phosphorylation ; Proteins ; Quinone oxidoreductase ; Reverse transcription ; Thymus ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; γ-Interferon</subject><ispartof>Molecular medicine reports, 2021-07, Vol.24 (1), Article 509</ispartof><rights>Copyright Spandidos Publications UK Ltd. 2021</rights><rights>Copyright: © Park et al. 2021</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-8fd67fe140bf694a64d00b4ecf1a01d57491a1b532d00267575a7e23f90d90673</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33982762$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Ji-Won</creatorcontrib><creatorcontrib>Oh, Jae-Hoon</creatorcontrib><creatorcontrib>Hwang, Daseul</creatorcontrib><creatorcontrib>Kim, Seong-Man</creatorcontrib><creatorcontrib>Min, Jae-Hong</creatorcontrib><creatorcontrib>Seo, Ji-Yun</creatorcontrib><creatorcontrib>Chun, Wanjoo</creatorcontrib><creatorcontrib>Lee, Hee Jae</creatorcontrib><creatorcontrib>Oh, Sei-Ryang</creatorcontrib><creatorcontrib>Lee, Jae-Won</creatorcontrib><creatorcontrib>Ahn, Kyung-Seop</creatorcontrib><title>3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>3,4,5‑Trihydroxycinnamic acid (THCA) exhibits anti‑inflammatory activity in acute or chronic inflammatory disorders, such as acute lung injury and asthma. The present study investigated the anti‑inflammatory activity of THCA in a tumor necrosis factor‑α/interferon‑γ (TI) mixture‑stimulated human keratinocyte cell line. The results of ELISA and reverse transcription‑quantitative PCR revealed that THCA reduced the secretion and mRNA expression levels of interleukin (IL)‑6; IL‑8; thymus and activation‑regulated chemokine; macrophage‑derived chemokine; regulated upon activation, normal T cell expressed and secreted; and monocyte chemoattractant protein‑1 in TI mixture‑stimulated HaCaT cells. In addition, the results of western blot analysis demonstrated that THCA exerted inhibitory activity on the activation of AKT, ERK and nuclear factor‑κB in TI mixture‑stimulated HaCaT cells. Furthermore, THCA upregulated the expression levels of heme oxygenase‑1 and NAD(P)H:quinone oxidoreductase 1, and the activation of nuclear factor erythroid 2‑related factor 2 in HaCaT cells. These results demonstrated that THCA may exhibit anti‑inflammatory activity in activated HaCaT cells.</description><subject>Acids</subject><subject>AKT protein</subject><subject>Anti-inflammatory agents</subject><subject>Asthma</subject><subject>CCL17 protein</subject><subject>CCL22 protein</subject><subject>Cell activation</subject><subject>Cell culture</subject><subject>Chemokines</subject><subject>Cytokines</subject><subject>Eczema</subject><subject>Gene expression</subject><subject>Heme</subject><subject>Heme oxygenase (decyclizing)</subject><subject>Inflammatory diseases</subject><subject>Interleukin 8</subject><subject>Kinases</subject><subject>Lymphocytes T</subject><subject>Monocyte chemoattractant protein</subject><subject>Monocyte chemoattractant protein 1</subject><subject>Monocytes</subject><subject>NAD</subject><subject>NADPH quinone oxidoreductase</subject><subject>Oxygenase</subject><subject>Phosphorylation</subject><subject>Proteins</subject><subject>Quinone oxidoreductase</subject><subject>Reverse transcription</subject><subject>Thymus</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>γ-Interferon</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkc9OVDEYxRuDEUS3LM1N2Lhghv7v7YaETBwhIbgZ102nt4WS23Zo7yXMzlfwUYzv4UP4JPbKSNRN-6Xn15Pv5ABwhOCctBKfhpDnGGI0RxjR9gU4QEKiGYGQ7u1mLKXYB69LuYOQM8zkK7BPiGyx4PgAbMgJPWE_v3xdZX-77XJ63Bofow7eNNr4rrGPNg-l0XHwlfLR9ToEPaS8baxz1lQtxWZ1vazqj2-nl8vrafhejzL4MPZ6sF1zoRd61Rjb9-UNeOl0X-zb3X0IPi8_rBYXs6tPHy8X51czQ1E7zFrXceEsonDtuKSa0w7CNbXGIQ1RxwSVSKM1I7i-Yy6YYFpYTJyEnYRckENw9uS7GdfBdsbGIetebbIPOm9V0l79q0R_q27Sg2oRoa3g1eD9ziCn-9GWQQVfpgg62jQWhRnmBDIucUWP_0Pv0phjjVcpwgVlLZyo-RNlciolW_e8DIJqKlPVMtVUpvpdZv3w7u8Iz_if9sgvvQOhNg</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Park, Ji-Won</creator><creator>Oh, Jae-Hoon</creator><creator>Hwang, Daseul</creator><creator>Kim, Seong-Man</creator><creator>Min, Jae-Hong</creator><creator>Seo, Ji-Yun</creator><creator>Chun, Wanjoo</creator><creator>Lee, Hee Jae</creator><creator>Oh, Sei-Ryang</creator><creator>Lee, Jae-Won</creator><creator>Ahn, Kyung-Seop</creator><general>Spandidos Publications UK Ltd</general><general>D.A. 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The present study investigated the anti‑inflammatory activity of THCA in a tumor necrosis factor‑α/interferon‑γ (TI) mixture‑stimulated human keratinocyte cell line. The results of ELISA and reverse transcription‑quantitative PCR revealed that THCA reduced the secretion and mRNA expression levels of interleukin (IL)‑6; IL‑8; thymus and activation‑regulated chemokine; macrophage‑derived chemokine; regulated upon activation, normal T cell expressed and secreted; and monocyte chemoattractant protein‑1 in TI mixture‑stimulated HaCaT cells. In addition, the results of western blot analysis demonstrated that THCA exerted inhibitory activity on the activation of AKT, ERK and nuclear factor‑κB in TI mixture‑stimulated HaCaT cells. Furthermore, THCA upregulated the expression levels of heme oxygenase‑1 and NAD(P)H:quinone oxidoreductase 1, and the activation of nuclear factor erythroid 2‑related factor 2 in HaCaT cells. These results demonstrated that THCA may exhibit anti‑inflammatory activity in activated HaCaT cells.</abstract><cop>Greece</cop><pub>Spandidos Publications UK Ltd</pub><pmid>33982762</pmid><doi>10.3892/mmr.2021.12148</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Acids AKT protein Anti-inflammatory agents Asthma CCL17 protein CCL22 protein Cell activation Cell culture Chemokines Cytokines Eczema Gene expression Heme Heme oxygenase (decyclizing) Inflammatory diseases Interleukin 8 Kinases Lymphocytes T Monocyte chemoattractant protein Monocyte chemoattractant protein 1 Monocytes NAD NADPH quinone oxidoreductase Oxygenase Phosphorylation Proteins Quinone oxidoreductase Reverse transcription Thymus Tumor necrosis factor-TNF Tumor necrosis factor-α γ-Interferon |
| title | 3,4,5‑Trihydroxycinnamic acid exerts anti‑inflammatory effects on TNF‑α/IFN‑γ‑stimulated HaCaT cells |
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