Ergosterol‐targeting fusion antifungal peptide significantly increases the Verticillium wilt resistance of cotton
Summary Increasing the targeting ability of antifungal proteins towards specific components of fungal cells has the potential to improve their antifungal activity and reduce harmful effects to nontarget cells. To obtain effective disease resistance genes against cotton Verticillium wilt, we construc...
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| Veröffentlicht in: | Plant biotechnology journal 2021-05, Vol.19 (5), p.926-936 |
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| creator | Tong, Sheng Yuan, Min Liu, Yu Li, Xianbi Jin, Dan Cheng, Xi Lin, Dongmei Ling, Haichun Yang, Danni Wang, Yang Mao, Ajing Pei, Yan Fan, Yanhua |
| description | Summary
Increasing the targeting ability of antifungal proteins towards specific components of fungal cells has the potential to improve their antifungal activity and reduce harmful effects to nontarget cells. To obtain effective disease resistance genes against cotton Verticillium wilt, we constructed several fusion genes, in which binding domains targeting chitin, sphingolipid or ergosterol in the fungal cell wall or cell membrane were individually fused to the antifungal peptide BbAFP1 from entomopathogenic fungus Beauveria bassiana. Transient expression of fusion genes in cotton cotyledons indicated that the BbAFP1::ErBD fusion peptide with an ergosterol binding domain exhibited better disease resistance against V. dahliae than wild‐type BbAFP1 and other fusion genes. BbAFP1::ErBD and BbAFP1 transgenic cotton were obtained and verified by Southern and Western blotting. Compared with BbAFP1‐expressing cotton, BbAFP1::ErBD‐expressing cotton showed higher disease resistance against V. dahliae, with smaller lesion areas (0.07 cm2 vs. 0.16 cm2) on the leaves and a lower disease index (23.9 vs. 34.5). Overexpression of BbAFP1::ErBD by transgenic tobacco also showed enhanced disease resistance against V. dahliae compared with that of the wild‐type gene. These results indicated that construction of fusion antifungal peptides that target fungal cells is a powerful strategy to obtain new anti‐disease genes, and the obtained fusion gene BbAFP1::ErBD has the potential to defend against plant fungal diseases. |
| doi_str_mv | 10.1111/pbi.13517 |
| format | Article |
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Increasing the targeting ability of antifungal proteins towards specific components of fungal cells has the potential to improve their antifungal activity and reduce harmful effects to nontarget cells. To obtain effective disease resistance genes against cotton Verticillium wilt, we constructed several fusion genes, in which binding domains targeting chitin, sphingolipid or ergosterol in the fungal cell wall or cell membrane were individually fused to the antifungal peptide BbAFP1 from entomopathogenic fungus Beauveria bassiana. Transient expression of fusion genes in cotton cotyledons indicated that the BbAFP1::ErBD fusion peptide with an ergosterol binding domain exhibited better disease resistance against V. dahliae than wild‐type BbAFP1 and other fusion genes. BbAFP1::ErBD and BbAFP1 transgenic cotton were obtained and verified by Southern and Western blotting. Compared with BbAFP1‐expressing cotton, BbAFP1::ErBD‐expressing cotton showed higher disease resistance against V. dahliae, with smaller lesion areas (0.07 cm2 vs. 0.16 cm2) on the leaves and a lower disease index (23.9 vs. 34.5). Overexpression of BbAFP1::ErBD by transgenic tobacco also showed enhanced disease resistance against V. dahliae compared with that of the wild‐type gene. These results indicated that construction of fusion antifungal peptides that target fungal cells is a powerful strategy to obtain new anti‐disease genes, and the obtained fusion gene BbAFP1::ErBD has the potential to defend against plant fungal diseases.</description><identifier>ISSN: 1467-7644</identifier><identifier>EISSN: 1467-7652</identifier><identifier>DOI: 10.1111/pbi.13517</identifier><identifier>PMID: 33217142</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Antibodies ; Antifungal activity ; Antifungal agents ; Antimicrobial agents ; Binding ; binding domain ; Cell fusion ; Cell membranes ; Cell walls ; Chitin ; Cotton ; Cotyledons ; Disease resistance ; Domains ; Entomopathogenic fungi ; Ergosterol ; Fungal diseases ; Fungi ; Fungicides ; fusion antifungal peptide ; Fusion protein ; Gene expression ; Gene fusion ; Genes ; Genetic engineering ; Glucose ; Membrane fusion ; Peptides ; Plant diseases ; Tobacco ; Toxicity ; Transgenic plants ; Verticillium ; Verticillium dahliae ; Verticillium wilt ; Western blotting</subject><ispartof>Plant biotechnology journal, 2021-05, Vol.19 (5), p.926-936</ispartof><rights>2020 The Authors. published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</rights><rights>2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4717-fff74556817321b138480a5a946512e1e38be79633181a158849bd2379974b833</citedby><cites>FETCH-LOGICAL-c4717-fff74556817321b138480a5a946512e1e38be79633181a158849bd2379974b833</cites><orcidid>0000-0001-8317-6199 ; 0000-0002-9298-2026 ; 0000-0003-1178-3998</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpbi.13517$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpbi.13517$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33217142$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tong, Sheng</creatorcontrib><creatorcontrib>Yuan, Min</creatorcontrib><creatorcontrib>Liu, Yu</creatorcontrib><creatorcontrib>Li, Xianbi</creatorcontrib><creatorcontrib>Jin, Dan</creatorcontrib><creatorcontrib>Cheng, Xi</creatorcontrib><creatorcontrib>Lin, Dongmei</creatorcontrib><creatorcontrib>Ling, Haichun</creatorcontrib><creatorcontrib>Yang, Danni</creatorcontrib><creatorcontrib>Wang, Yang</creatorcontrib><creatorcontrib>Mao, Ajing</creatorcontrib><creatorcontrib>Pei, Yan</creatorcontrib><creatorcontrib>Fan, Yanhua</creatorcontrib><title>Ergosterol‐targeting fusion antifungal peptide significantly increases the Verticillium wilt resistance of cotton</title><title>Plant biotechnology journal</title><addtitle>Plant Biotechnol J</addtitle><description>Summary
Increasing the targeting ability of antifungal proteins towards specific components of fungal cells has the potential to improve their antifungal activity and reduce harmful effects to nontarget cells. To obtain effective disease resistance genes against cotton Verticillium wilt, we constructed several fusion genes, in which binding domains targeting chitin, sphingolipid or ergosterol in the fungal cell wall or cell membrane were individually fused to the antifungal peptide BbAFP1 from entomopathogenic fungus Beauveria bassiana. Transient expression of fusion genes in cotton cotyledons indicated that the BbAFP1::ErBD fusion peptide with an ergosterol binding domain exhibited better disease resistance against V. dahliae than wild‐type BbAFP1 and other fusion genes. BbAFP1::ErBD and BbAFP1 transgenic cotton were obtained and verified by Southern and Western blotting. Compared with BbAFP1‐expressing cotton, BbAFP1::ErBD‐expressing cotton showed higher disease resistance against V. dahliae, with smaller lesion areas (0.07 cm2 vs. 0.16 cm2) on the leaves and a lower disease index (23.9 vs. 34.5). Overexpression of BbAFP1::ErBD by transgenic tobacco also showed enhanced disease resistance against V. dahliae compared with that of the wild‐type gene. These results indicated that construction of fusion antifungal peptides that target fungal cells is a powerful strategy to obtain new anti‐disease genes, and the obtained fusion gene BbAFP1::ErBD has the potential to defend against plant fungal diseases.</description><subject>Antibodies</subject><subject>Antifungal activity</subject><subject>Antifungal agents</subject><subject>Antimicrobial agents</subject><subject>Binding</subject><subject>binding domain</subject><subject>Cell fusion</subject><subject>Cell membranes</subject><subject>Cell walls</subject><subject>Chitin</subject><subject>Cotton</subject><subject>Cotyledons</subject><subject>Disease resistance</subject><subject>Domains</subject><subject>Entomopathogenic fungi</subject><subject>Ergosterol</subject><subject>Fungal diseases</subject><subject>Fungi</subject><subject>Fungicides</subject><subject>fusion antifungal peptide</subject><subject>Fusion protein</subject><subject>Gene expression</subject><subject>Gene fusion</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Glucose</subject><subject>Membrane fusion</subject><subject>Peptides</subject><subject>Plant diseases</subject><subject>Tobacco</subject><subject>Toxicity</subject><subject>Transgenic plants</subject><subject>Verticillium</subject><subject>Verticillium dahliae</subject><subject>Verticillium wilt</subject><subject>Western blotting</subject><issn>1467-7644</issn><issn>1467-7652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc9qFTEUhwdRbK0ufAEJuLGL287J_9kIWqoWCrpQtyEz98w0JTcZk4xydz5Cn9EnMXrrRQXN5gTOx4_f4Wuax9CeQH2nc-9OgAlQd5pD4FKtlBT07v7P-UHzIOfrtqUghbzfHDBGQQGnh00-T1PMBVP0377eFJsmLC5MZFyyi4HYUNy4hMl6MuNc3BpJdlNwoxvqym-JC0NCmzGTcoXkI6biBue9Wzbki_OFJMwuFxsGJHEkQywlhofNvdH6jI9u51Hz4dX5-7M3q8u3ry_OXlyuBq5ArcZxVFwIqUHVuj0wzXVrhe24FEARkOkeVScZAw0WhNa869eUqa5TvNeMHTXPd7nz0m9wPWAoyXozJ7exaWuidebPTXBXZoqfjQYGLec14NltQIqfFszFbFwe0HsbMC7ZUC4r2GkqKvr0L_Q6LinU8wwDoLrV0On_UVTQ2p8KkJU63lFDijknHPeVoTU_hJsq3PwUXtknv9-4J38ZrsDpDqg6cPvvJPPu5cUu8jtkI7Z8</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Tong, Sheng</creator><creator>Yuan, Min</creator><creator>Liu, Yu</creator><creator>Li, Xianbi</creator><creator>Jin, Dan</creator><creator>Cheng, Xi</creator><creator>Lin, Dongmei</creator><creator>Ling, Haichun</creator><creator>Yang, Danni</creator><creator>Wang, Yang</creator><creator>Mao, Ajing</creator><creator>Pei, Yan</creator><creator>Fan, Yanhua</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8317-6199</orcidid><orcidid>https://orcid.org/0000-0002-9298-2026</orcidid><orcidid>https://orcid.org/0000-0003-1178-3998</orcidid></search><sort><creationdate>202105</creationdate><title>Ergosterol‐targeting fusion antifungal peptide significantly increases the Verticillium wilt resistance of cotton</title><author>Tong, Sheng ; 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Increasing the targeting ability of antifungal proteins towards specific components of fungal cells has the potential to improve their antifungal activity and reduce harmful effects to nontarget cells. To obtain effective disease resistance genes against cotton Verticillium wilt, we constructed several fusion genes, in which binding domains targeting chitin, sphingolipid or ergosterol in the fungal cell wall or cell membrane were individually fused to the antifungal peptide BbAFP1 from entomopathogenic fungus Beauveria bassiana. Transient expression of fusion genes in cotton cotyledons indicated that the BbAFP1::ErBD fusion peptide with an ergosterol binding domain exhibited better disease resistance against V. dahliae than wild‐type BbAFP1 and other fusion genes. BbAFP1::ErBD and BbAFP1 transgenic cotton were obtained and verified by Southern and Western blotting. Compared with BbAFP1‐expressing cotton, BbAFP1::ErBD‐expressing cotton showed higher disease resistance against V. dahliae, with smaller lesion areas (0.07 cm2 vs. 0.16 cm2) on the leaves and a lower disease index (23.9 vs. 34.5). Overexpression of BbAFP1::ErBD by transgenic tobacco also showed enhanced disease resistance against V. dahliae compared with that of the wild‐type gene. These results indicated that construction of fusion antifungal peptides that target fungal cells is a powerful strategy to obtain new anti‐disease genes, and the obtained fusion gene BbAFP1::ErBD has the potential to defend against plant fungal diseases.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>33217142</pmid><doi>10.1111/pbi.13517</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8317-6199</orcidid><orcidid>https://orcid.org/0000-0002-9298-2026</orcidid><orcidid>https://orcid.org/0000-0003-1178-3998</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Antifungal activity Antifungal agents Antimicrobial agents Binding binding domain Cell fusion Cell membranes Cell walls Chitin Cotton Cotyledons Disease resistance Domains Entomopathogenic fungi Ergosterol Fungal diseases Fungi Fungicides fusion antifungal peptide Fusion protein Gene expression Gene fusion Genes Genetic engineering Glucose Membrane fusion Peptides Plant diseases Tobacco Toxicity Transgenic plants Verticillium Verticillium dahliae Verticillium wilt Western blotting |
| title | Ergosterol‐targeting fusion antifungal peptide significantly increases the Verticillium wilt resistance of cotton |
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