An improved in vivo tethering assay with single molecule FISH reveals that a nematode Nanos enhances reporter expression and mRNA stability

Robust methods are critical for testing the in vivo regulatory mechanism of RNA binding proteins. Here we report improvement of a protein-mRNA tethering assay to probe the function of an RNA binding protein in its natural context within the adult germline. The assay relies on a dual reporter express...

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Veröffentlicht in:	RNA (Cambridge) 2021-06, Vol.27 (6), p.643-652
Hauptverfasser:	Doenier, Jonathan, Lynch, Tina R, Kimble, Judith, Aoki, Scott T
Format:	Artikel
Sprache:	eng
Schlagworte:	3' Untranslated regions Cell fate Gene expression Immunofluorescence Kinases mRNA stability Nanos protein Post-transcription Proteins Quantitation RNA-binding protein Zinc finger proteins
Online-Zugang:	Volltext
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