Optimization of immobilization of Pseudomonas cepacia lipase on multiwalled carbon nanotubes functionalized with glycyrrhizin and Tween 80
In the present study, multiwalled carbon nanotubes (MWCNTs) were functionalized with glycyrrhizin and Tween 80 and applied for immobilization of Pseudomonas cepacia lipase ( Pc L). Characterization of f-MWCNTs was performed through Fourier-transform infrared spectroscopy, thermal gravimetric, field...
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| Veröffentlicht in: | 3 Biotech 2021-06, Vol.11 (6), p.260-260, Article 260 |
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| creator | Ameri, Atefeh Forootanfar, Hamid Behnam, Behzad Shakibaie, Mojtaba Ameri, Alieh Daneshpajooh, Mohammad Najafi, Amir Amirheidari, Bagher |
| description | In the present study, multiwalled carbon nanotubes (MWCNTs) were functionalized with glycyrrhizin and Tween 80 and applied for immobilization of
Pseudomonas cepacia
lipase (
Pc
L). Characterization of f-MWCNTs was performed through Fourier-transform infrared spectroscopy, thermal gravimetric, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy analysis. The optimum specific activity of immobilized
Pc
L (studied by Plackett–Burman statistical design) occurred at 0.3 mg/mL of f-MWCNTs, 25 mM of phosphate buffer (pH 6.0), 15 min sonication time, 8 U/mL of enzyme concentration, and 24 h immobilization time at 4 °C in the absence of glutaraldehyde. In these conditions, the specific activity was 16.57 ± 0.71 U/mg, which was very close to the predicted amount (16.62 ± 0.64 U/mg). The results of thermal and pH stability showed that the stability of immobilized
Pc
L was higher than that of the free
Pc
L. The activity of immobilized
Pc
L on f-MWCNTs held 93% after being incubated for 60 min at 70 °C. Moreover, the immobilized
Pc
L on f-MWCNTs retained about 65% of its initial activity after 30 days of storage at 25 °C. In addition, about 50% of initial activity of immobilized
Pc
L retained after 10 cycles of uses. Therefore, f-MWCNTs could be introduced as suitable support for enzymes immobilization. |
| doi_str_mv | 10.1007/s13205-021-02813-9 |
| format | Article |
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Pseudomonas cepacia
lipase (
Pc
L). Characterization of f-MWCNTs was performed through Fourier-transform infrared spectroscopy, thermal gravimetric, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy analysis. The optimum specific activity of immobilized
Pc
L (studied by Plackett–Burman statistical design) occurred at 0.3 mg/mL of f-MWCNTs, 25 mM of phosphate buffer (pH 6.0), 15 min sonication time, 8 U/mL of enzyme concentration, and 24 h immobilization time at 4 °C in the absence of glutaraldehyde. In these conditions, the specific activity was 16.57 ± 0.71 U/mg, which was very close to the predicted amount (16.62 ± 0.64 U/mg). The results of thermal and pH stability showed that the stability of immobilized
Pc
L was higher than that of the free
Pc
L. The activity of immobilized
Pc
L on f-MWCNTs held 93% after being incubated for 60 min at 70 °C. Moreover, the immobilized
Pc
L on f-MWCNTs retained about 65% of its initial activity after 30 days of storage at 25 °C. In addition, about 50% of initial activity of immobilized
Pc
L retained after 10 cycles of uses. Therefore, f-MWCNTs could be introduced as suitable support for enzymes immobilization.</description><identifier>ISSN: 2190-572X</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-021-02813-9</identifier><identifier>PMID: 33996372</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Agriculture ; Bioinformatics ; Biomaterials ; Biotechnology ; Burkholderia cepacia ; Cancer Research ; carbon nanotubes ; carboxylic ester hydrolases ; Chemistry ; Chemistry and Materials Science ; electron microscopy ; Emission analysis ; energy-dispersive X-ray analysis ; Field emission microscopy ; Field emission spectroscopy ; Fourier transform infrared spectroscopy ; Fourier transforms ; glutaraldehyde ; Glycyrrhizin ; Gravimetric analysis ; Immobilization ; Infrared spectroscopy ; Lipase ; Multi wall carbon nanotubes ; Nanotubes ; Optimization ; Original ; Original Article ; pH effects ; pH stability ; phosphates ; polysorbates ; Pseudomonas ; Pseudomonas cepacia ; Scanning electron microscopy ; Sonication ; Spectrum analysis ; Stability ; Stem Cells ; X-ray spectroscopy</subject><ispartof>3 Biotech, 2021-06, Vol.11 (6), p.260-260, Article 260</ispartof><rights>King Abdulaziz City for Science and Technology 2021</rights><rights>King Abdulaziz City for Science and Technology 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-faf3bbf1315a3352e9bb4d4a7cca427a4a15f67cb57a99bde4d9987e5d7cc9a93</citedby><cites>FETCH-LOGICAL-c507t-faf3bbf1315a3352e9bb4d4a7cca427a4a15f67cb57a99bde4d9987e5d7cc9a93</cites><orcidid>0000-0001-5049-7674</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110682/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110682/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,41469,42538,51300,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33996372$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ameri, Atefeh</creatorcontrib><creatorcontrib>Forootanfar, Hamid</creatorcontrib><creatorcontrib>Behnam, Behzad</creatorcontrib><creatorcontrib>Shakibaie, Mojtaba</creatorcontrib><creatorcontrib>Ameri, Alieh</creatorcontrib><creatorcontrib>Daneshpajooh, Mohammad</creatorcontrib><creatorcontrib>Najafi, Amir</creatorcontrib><creatorcontrib>Amirheidari, Bagher</creatorcontrib><title>Optimization of immobilization of Pseudomonas cepacia lipase on multiwalled carbon nanotubes functionalized with glycyrrhizin and Tween 80</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>In the present study, multiwalled carbon nanotubes (MWCNTs) were functionalized with glycyrrhizin and Tween 80 and applied for immobilization of
Pseudomonas cepacia
lipase (
Pc
L). Characterization of f-MWCNTs was performed through Fourier-transform infrared spectroscopy, thermal gravimetric, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy analysis. The optimum specific activity of immobilized
Pc
L (studied by Plackett–Burman statistical design) occurred at 0.3 mg/mL of f-MWCNTs, 25 mM of phosphate buffer (pH 6.0), 15 min sonication time, 8 U/mL of enzyme concentration, and 24 h immobilization time at 4 °C in the absence of glutaraldehyde. In these conditions, the specific activity was 16.57 ± 0.71 U/mg, which was very close to the predicted amount (16.62 ± 0.64 U/mg). The results of thermal and pH stability showed that the stability of immobilized
Pc
L was higher than that of the free
Pc
L. The activity of immobilized
Pc
L on f-MWCNTs held 93% after being incubated for 60 min at 70 °C. Moreover, the immobilized
Pc
L on f-MWCNTs retained about 65% of its initial activity after 30 days of storage at 25 °C. In addition, about 50% of initial activity of immobilized
Pc
L retained after 10 cycles of uses. Therefore, f-MWCNTs could be introduced as suitable support for enzymes immobilization.</description><subject>Agriculture</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Burkholderia cepacia</subject><subject>Cancer Research</subject><subject>carbon nanotubes</subject><subject>carboxylic ester hydrolases</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>electron microscopy</subject><subject>Emission analysis</subject><subject>energy-dispersive X-ray analysis</subject><subject>Field emission microscopy</subject><subject>Field emission spectroscopy</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Fourier transforms</subject><subject>glutaraldehyde</subject><subject>Glycyrrhizin</subject><subject>Gravimetric analysis</subject><subject>Immobilization</subject><subject>Infrared spectroscopy</subject><subject>Lipase</subject><subject>Multi wall carbon nanotubes</subject><subject>Nanotubes</subject><subject>Optimization</subject><subject>Original</subject><subject>Original Article</subject><subject>pH effects</subject><subject>pH stability</subject><subject>phosphates</subject><subject>polysorbates</subject><subject>Pseudomonas</subject><subject>Pseudomonas cepacia</subject><subject>Scanning electron microscopy</subject><subject>Sonication</subject><subject>Spectrum analysis</subject><subject>Stability</subject><subject>Stem Cells</subject><subject>X-ray spectroscopy</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqNks9qFTEUxgdRbKl9ARcScONmNH8mN5ONIEVboVAXFdyFk0zm3pRMMiYzvdw-gk9tprdeqwsxEHI453e-nISvql4S_JZgLN5lwijmNaak7JawWj6pjimRuOaCtU8PMf12VJ3mfIPL4oRLgp9XR4xJuWKCHlc_rsbJDe4OJhcDij1ywxC1848yX7KduzjEABkZO4JxgLwbIVtUgGH2k9uC97ZDBpIuqQAhTrO2GfVzMIsMFL1S37ppg9Z-Z3YpbdydCwhCh6631gbU4hfVsx58tqcP50n19dPH67OL-vLq_PPZh8vacCymuoeead0TRjgwxqmVWjddA8IYaKiABgjvV8JoLkBK3dmmk7IVlneFkCDZSfV-rzvOerCdsWFK4NWY3ABppyI49WcluI1ax1vVEoJXLS0Cbx4EUvw-2zypwWVjvYdg45wV5ZzIlZSN-A-Utg2jAi_o67_Qmzin8nX3VCOJbNpleLqnTIo5J9sf5iZYLcZQe2OoYgx1bwy1NL16_OJDyy8bFIDtgVxKYW3T77v_IfsTcWDH_Q</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Ameri, Atefeh</creator><creator>Forootanfar, Hamid</creator><creator>Behnam, Behzad</creator><creator>Shakibaie, Mojtaba</creator><creator>Ameri, Alieh</creator><creator>Daneshpajooh, Mohammad</creator><creator>Najafi, Amir</creator><creator>Amirheidari, Bagher</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5049-7674</orcidid></search><sort><creationdate>20210601</creationdate><title>Optimization of immobilization of Pseudomonas cepacia lipase on multiwalled carbon nanotubes functionalized with glycyrrhizin and Tween 80</title><author>Ameri, Atefeh ; Forootanfar, Hamid ; Behnam, Behzad ; Shakibaie, Mojtaba ; Ameri, Alieh ; Daneshpajooh, Mohammad ; Najafi, Amir ; Amirheidari, Bagher</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-faf3bbf1315a3352e9bb4d4a7cca427a4a15f67cb57a99bde4d9987e5d7cc9a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agriculture</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Burkholderia cepacia</topic><topic>Cancer Research</topic><topic>carbon nanotubes</topic><topic>carboxylic ester hydrolases</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>electron microscopy</topic><topic>Emission analysis</topic><topic>energy-dispersive X-ray analysis</topic><topic>Field emission microscopy</topic><topic>Field emission spectroscopy</topic><topic>Fourier transform infrared spectroscopy</topic><topic>Fourier transforms</topic><topic>glutaraldehyde</topic><topic>Glycyrrhizin</topic><topic>Gravimetric analysis</topic><topic>Immobilization</topic><topic>Infrared spectroscopy</topic><topic>Lipase</topic><topic>Multi wall carbon nanotubes</topic><topic>Nanotubes</topic><topic>Optimization</topic><topic>Original</topic><topic>Original Article</topic><topic>pH effects</topic><topic>pH stability</topic><topic>phosphates</topic><topic>polysorbates</topic><topic>Pseudomonas</topic><topic>Pseudomonas cepacia</topic><topic>Scanning electron microscopy</topic><topic>Sonication</topic><topic>Spectrum analysis</topic><topic>Stability</topic><topic>Stem Cells</topic><topic>X-ray spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ameri, Atefeh</creatorcontrib><creatorcontrib>Forootanfar, Hamid</creatorcontrib><creatorcontrib>Behnam, Behzad</creatorcontrib><creatorcontrib>Shakibaie, Mojtaba</creatorcontrib><creatorcontrib>Ameri, Alieh</creatorcontrib><creatorcontrib>Daneshpajooh, Mohammad</creatorcontrib><creatorcontrib>Najafi, Amir</creatorcontrib><creatorcontrib>Amirheidari, Bagher</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ameri, Atefeh</au><au>Forootanfar, Hamid</au><au>Behnam, Behzad</au><au>Shakibaie, Mojtaba</au><au>Ameri, Alieh</au><au>Daneshpajooh, Mohammad</au><au>Najafi, Amir</au><au>Amirheidari, Bagher</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of immobilization of Pseudomonas cepacia lipase on multiwalled carbon nanotubes functionalized with glycyrrhizin and Tween 80</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2021-06-01</date><risdate>2021</risdate><volume>11</volume><issue>6</issue><spage>260</spage><epage>260</epage><pages>260-260</pages><artnum>260</artnum><issn>2190-572X</issn><eissn>2190-5738</eissn><abstract>In the present study, multiwalled carbon nanotubes (MWCNTs) were functionalized with glycyrrhizin and Tween 80 and applied for immobilization of
Pseudomonas cepacia
lipase (
Pc
L). Characterization of f-MWCNTs was performed through Fourier-transform infrared spectroscopy, thermal gravimetric, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy analysis. The optimum specific activity of immobilized
Pc
L (studied by Plackett–Burman statistical design) occurred at 0.3 mg/mL of f-MWCNTs, 25 mM of phosphate buffer (pH 6.0), 15 min sonication time, 8 U/mL of enzyme concentration, and 24 h immobilization time at 4 °C in the absence of glutaraldehyde. In these conditions, the specific activity was 16.57 ± 0.71 U/mg, which was very close to the predicted amount (16.62 ± 0.64 U/mg). The results of thermal and pH stability showed that the stability of immobilized
Pc
L was higher than that of the free
Pc
L. The activity of immobilized
Pc
L on f-MWCNTs held 93% after being incubated for 60 min at 70 °C. Moreover, the immobilized
Pc
L on f-MWCNTs retained about 65% of its initial activity after 30 days of storage at 25 °C. In addition, about 50% of initial activity of immobilized
Pc
L retained after 10 cycles of uses. Therefore, f-MWCNTs could be introduced as suitable support for enzymes immobilization.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>33996372</pmid><doi>10.1007/s13205-021-02813-9</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5049-7674</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Agriculture Bioinformatics Biomaterials Biotechnology Burkholderia cepacia Cancer Research carbon nanotubes carboxylic ester hydrolases Chemistry Chemistry and Materials Science electron microscopy Emission analysis energy-dispersive X-ray analysis Field emission microscopy Field emission spectroscopy Fourier transform infrared spectroscopy Fourier transforms glutaraldehyde Glycyrrhizin Gravimetric analysis Immobilization Infrared spectroscopy Lipase Multi wall carbon nanotubes Nanotubes Optimization Original Original Article pH effects pH stability phosphates polysorbates Pseudomonas Pseudomonas cepacia Scanning electron microscopy Sonication Spectrum analysis Stability Stem Cells X-ray spectroscopy |
| title | Optimization of immobilization of Pseudomonas cepacia lipase on multiwalled carbon nanotubes functionalized with glycyrrhizin and Tween 80 |
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