Molecular characterization of Feline paramyxovirus and Feline morbillivirus in cats from Brazil
This study is aimed at detecting Feline paramyxovirus (FPaV) and Feline morbillivirus (FeMV) in 35 urine samples from domestic cats, collected in 2019, with or without clinical signs of uropathies using a reverse transcription-polymerase chain reaction (RT-PCR) followed by semi-nested polymerase cha...
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| Veröffentlicht in: | Brazilian journal of microbiology 2021-06, Vol.52 (2), p.961-965 |
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| container_title | Brazilian journal of microbiology |
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| creator | Balbo, Luciana C. Fritzen, Juliana T. T. Lorenzetti, Elis Medeiros, Thais N. S. Jardim, Andressa M. Alfieri, Amauri A. Alfieri, Alice F. |
| description | This study is aimed at detecting
Feline paramyxovirus
(FPaV) and
Feline morbillivirus
(FeMV) in 35 urine samples from domestic cats, collected in 2019, with or without clinical signs of uropathies using a reverse transcription-polymerase chain reaction (RT-PCR) followed by semi-nested polymerase chain reaction (SN-PCR) assays to amplify a partial paramyxovirus L gene. Eight (22.9%) out of the 35 urine samples were positive for paramyxoviruses. Sequencing and phylogenetic analyses revealed that three samples were positive for FPaV, four samples were positive for FeMV, and it was not possible to determine which virus was present in one RT-SN-PCR positive urine sample. FPaV strains showed 100% nucleotide (nt) identity with each other and 97% nt identity with a Japanese 163 FPaV strain. The FeMV strains showed 85.9% nt identity with each other; three strains were similar to previously described Brazilian FeMV strains, and one strain clustered in a different branch of the phylogenetic tree together with the first described Chinese FeMV strain. This study provides the first description of FPaV strains in cats from Brazil and provides new information about the molecular characteristics of FPaV and FeMV strains circulating in domestic cats in Brazil. |
| doi_str_mv | 10.1007/s42770-021-00434-1 |
| format | Article |
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Feline paramyxovirus
(FPaV) and
Feline morbillivirus
(FeMV) in 35 urine samples from domestic cats, collected in 2019, with or without clinical signs of uropathies using a reverse transcription-polymerase chain reaction (RT-PCR) followed by semi-nested polymerase chain reaction (SN-PCR) assays to amplify a partial paramyxovirus L gene. Eight (22.9%) out of the 35 urine samples were positive for paramyxoviruses. Sequencing and phylogenetic analyses revealed that three samples were positive for FPaV, four samples were positive for FeMV, and it was not possible to determine which virus was present in one RT-SN-PCR positive urine sample. FPaV strains showed 100% nucleotide (nt) identity with each other and 97% nt identity with a Japanese 163 FPaV strain. The FeMV strains showed 85.9% nt identity with each other; three strains were similar to previously described Brazilian FeMV strains, and one strain clustered in a different branch of the phylogenetic tree together with the first described Chinese FeMV strain. This study provides the first description of FPaV strains in cats from Brazil and provides new information about the molecular characteristics of FPaV and FeMV strains circulating in domestic cats in Brazil.</description><identifier>ISSN: 1517-8382</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-021-00434-1</identifier><identifier>PMID: 33483894</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animals ; Animals, Domestic ; Biomedical and Life Sciences ; Brazil - epidemiology ; Cat Diseases - epidemiology ; Cat Diseases - urine ; Cat Diseases - virology ; Cats ; Domestic animals ; Food Microbiology ; L gene ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Morbillivirus - classification ; Morbillivirus - genetics ; Morbillivirus - isolation & purification ; Mycology ; Nucleotides ; Paramyxoviridae - classification ; Paramyxoviridae - genetics ; Paramyxoviridae - isolation & purification ; Paramyxoviridae Infections - epidemiology ; Paramyxoviridae Infections - urine ; Paramyxoviridae Infections - veterinary ; Paramyxoviridae Infections - virology ; Phylogenetics ; Phylogeny ; Polymerase chain reaction ; Reverse transcription ; RNA, Viral - genetics ; Sequence Analysis, DNA ; Strains (organisms) ; Urine ; Veterinary Microbiology - Research Paper ; Viral Proteins - genetics ; Viruses</subject><ispartof>Brazilian journal of microbiology, 2021-06, Vol.52 (2), p.961-965</ispartof><rights>Sociedade Brasileira de Microbiologia 2021</rights><rights>Sociedade Brasileira de Microbiologia 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-b0523ff83066397efe58c5ed7b7e18fdc9a4d0184ba2319d23113376e5ed4b713</citedby><cites>FETCH-LOGICAL-c474t-b0523ff83066397efe58c5ed7b7e18fdc9a4d0184ba2319d23113376e5ed4b713</cites><orcidid>0000-0002-5032-712X ; 0000-0001-5303-0949 ; 0000-0003-4635-5512 ; 0000-0002-8607-956X ; 0000-0002-2353-182X ; 0000-0003-2892-5593 ; 0000-0002-7024-4487</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105471/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105471/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,41486,42555,51317,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33483894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Balbo, Luciana C.</creatorcontrib><creatorcontrib>Fritzen, Juliana T. T.</creatorcontrib><creatorcontrib>Lorenzetti, Elis</creatorcontrib><creatorcontrib>Medeiros, Thais N. S.</creatorcontrib><creatorcontrib>Jardim, Andressa M.</creatorcontrib><creatorcontrib>Alfieri, Amauri A.</creatorcontrib><creatorcontrib>Alfieri, Alice F.</creatorcontrib><title>Molecular characterization of Feline paramyxovirus and Feline morbillivirus in cats from Brazil</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>This study is aimed at detecting
Feline paramyxovirus
(FPaV) and
Feline morbillivirus
(FeMV) in 35 urine samples from domestic cats, collected in 2019, with or without clinical signs of uropathies using a reverse transcription-polymerase chain reaction (RT-PCR) followed by semi-nested polymerase chain reaction (SN-PCR) assays to amplify a partial paramyxovirus L gene. Eight (22.9%) out of the 35 urine samples were positive for paramyxoviruses. Sequencing and phylogenetic analyses revealed that three samples were positive for FPaV, four samples were positive for FeMV, and it was not possible to determine which virus was present in one RT-SN-PCR positive urine sample. FPaV strains showed 100% nucleotide (nt) identity with each other and 97% nt identity with a Japanese 163 FPaV strain. The FeMV strains showed 85.9% nt identity with each other; three strains were similar to previously described Brazilian FeMV strains, and one strain clustered in a different branch of the phylogenetic tree together with the first described Chinese FeMV strain. This study provides the first description of FPaV strains in cats from Brazil and provides new information about the molecular characteristics of FPaV and FeMV strains circulating in domestic cats in Brazil.</description><subject>Animals</subject><subject>Animals, Domestic</subject><subject>Biomedical and Life Sciences</subject><subject>Brazil - epidemiology</subject><subject>Cat Diseases - epidemiology</subject><subject>Cat Diseases - urine</subject><subject>Cat Diseases - virology</subject><subject>Cats</subject><subject>Domestic animals</subject><subject>Food Microbiology</subject><subject>L gene</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Morbillivirus - classification</subject><subject>Morbillivirus - genetics</subject><subject>Morbillivirus - isolation & purification</subject><subject>Mycology</subject><subject>Nucleotides</subject><subject>Paramyxoviridae - classification</subject><subject>Paramyxoviridae - genetics</subject><subject>Paramyxoviridae - isolation & purification</subject><subject>Paramyxoviridae Infections - epidemiology</subject><subject>Paramyxoviridae Infections - urine</subject><subject>Paramyxoviridae Infections - veterinary</subject><subject>Paramyxoviridae Infections - virology</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Reverse transcription</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Strains (organisms)</subject><subject>Urine</subject><subject>Veterinary Microbiology - Research Paper</subject><subject>Viral Proteins - genetics</subject><subject>Viruses</subject><issn>1517-8382</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9vFSEUxYnR2Fr9Ai7MJG7cjF7gzoO3MdHGtiY1bnRNGOZOS8PAE2Ya208v7bT1z8INEM6Pw7k5jL3k8JYDqHcFhVLQguAtAEps-SO2zzdKt4jQPa7njqtWSy322LNSLgBEByiesj0psV5vcZ-ZLymQW4LNjTu32bqZsr-2s0-xSWNzRMFHanZVma5-pkufl9LYONwLU8q9D8Gvgo-Ns3Npxpym5mO21z48Z09GGwq9uNsP2PejT98OT9rTr8efDz-ctg4Vzm0PnZDjqCVsNnKraKROu44G1Sviehzc1uIAXGNvheTboS5cSrWhymCvuDxg71ff3dJPNDiKc7bB7LKfbL4yyXrztxL9uTlLl0Zz6PDW4M2dQU4_FiqzmXxxFIKNlJZiBGrArkbQFX39D3qRlhzreEbUMThyiaJSYqVcTqVkGh_CcDA3_Zm1P1P7M7f9mZsUr_4c4-HJfWEVkCtQqhTPKP_--z-2vwCSPad4</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Balbo, Luciana C.</creator><creator>Fritzen, Juliana T. T.</creator><creator>Lorenzetti, Elis</creator><creator>Medeiros, Thais N. 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T. ; Lorenzetti, Elis ; Medeiros, Thais N. S. ; Jardim, Andressa M. ; Alfieri, Amauri A. ; Alfieri, Alice F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-b0523ff83066397efe58c5ed7b7e18fdc9a4d0184ba2319d23113376e5ed4b713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Animals, Domestic</topic><topic>Biomedical and Life Sciences</topic><topic>Brazil - epidemiology</topic><topic>Cat Diseases - epidemiology</topic><topic>Cat Diseases - urine</topic><topic>Cat Diseases - virology</topic><topic>Cats</topic><topic>Domestic animals</topic><topic>Food Microbiology</topic><topic>L gene</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Morbillivirus - classification</topic><topic>Morbillivirus - genetics</topic><topic>Morbillivirus - isolation & purification</topic><topic>Mycology</topic><topic>Nucleotides</topic><topic>Paramyxoviridae - classification</topic><topic>Paramyxoviridae - genetics</topic><topic>Paramyxoviridae - isolation & purification</topic><topic>Paramyxoviridae Infections - epidemiology</topic><topic>Paramyxoviridae Infections - urine</topic><topic>Paramyxoviridae Infections - veterinary</topic><topic>Paramyxoviridae Infections - virology</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Reverse transcription</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Strains (organisms)</topic><topic>Urine</topic><topic>Veterinary Microbiology - Research Paper</topic><topic>Viral Proteins - genetics</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Balbo, Luciana C.</creatorcontrib><creatorcontrib>Fritzen, Juliana T. T.</creatorcontrib><creatorcontrib>Lorenzetti, Elis</creatorcontrib><creatorcontrib>Medeiros, Thais N. S.</creatorcontrib><creatorcontrib>Jardim, Andressa M.</creatorcontrib><creatorcontrib>Alfieri, Amauri A.</creatorcontrib><creatorcontrib>Alfieri, Alice F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Balbo, Luciana C.</au><au>Fritzen, Juliana T. T.</au><au>Lorenzetti, Elis</au><au>Medeiros, Thais N. S.</au><au>Jardim, Andressa M.</au><au>Alfieri, Amauri A.</au><au>Alfieri, Alice F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of Feline paramyxovirus and Feline morbillivirus in cats from Brazil</atitle><jtitle>Brazilian journal of microbiology</jtitle><stitle>Braz J Microbiol</stitle><addtitle>Braz J Microbiol</addtitle><date>2021-06-01</date><risdate>2021</risdate><volume>52</volume><issue>2</issue><spage>961</spage><epage>965</epage><pages>961-965</pages><issn>1517-8382</issn><eissn>1678-4405</eissn><abstract>This study is aimed at detecting
Feline paramyxovirus
(FPaV) and
Feline morbillivirus
(FeMV) in 35 urine samples from domestic cats, collected in 2019, with or without clinical signs of uropathies using a reverse transcription-polymerase chain reaction (RT-PCR) followed by semi-nested polymerase chain reaction (SN-PCR) assays to amplify a partial paramyxovirus L gene. Eight (22.9%) out of the 35 urine samples were positive for paramyxoviruses. Sequencing and phylogenetic analyses revealed that three samples were positive for FPaV, four samples were positive for FeMV, and it was not possible to determine which virus was present in one RT-SN-PCR positive urine sample. FPaV strains showed 100% nucleotide (nt) identity with each other and 97% nt identity with a Japanese 163 FPaV strain. The FeMV strains showed 85.9% nt identity with each other; three strains were similar to previously described Brazilian FeMV strains, and one strain clustered in a different branch of the phylogenetic tree together with the first described Chinese FeMV strain. This study provides the first description of FPaV strains in cats from Brazil and provides new information about the molecular characteristics of FPaV and FeMV strains circulating in domestic cats in Brazil.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>33483894</pmid><doi>10.1007/s42770-021-00434-1</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-5032-712X</orcidid><orcidid>https://orcid.org/0000-0001-5303-0949</orcidid><orcidid>https://orcid.org/0000-0003-4635-5512</orcidid><orcidid>https://orcid.org/0000-0002-8607-956X</orcidid><orcidid>https://orcid.org/0000-0002-2353-182X</orcidid><orcidid>https://orcid.org/0000-0003-2892-5593</orcidid><orcidid>https://orcid.org/0000-0002-7024-4487</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Domestic Biomedical and Life Sciences Brazil - epidemiology Cat Diseases - epidemiology Cat Diseases - urine Cat Diseases - virology Cats Domestic animals Food Microbiology L gene Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Morbillivirus - classification Morbillivirus - genetics Morbillivirus - isolation & purification Mycology Nucleotides Paramyxoviridae - classification Paramyxoviridae - genetics Paramyxoviridae - isolation & purification Paramyxoviridae Infections - epidemiology Paramyxoviridae Infections - urine Paramyxoviridae Infections - veterinary Paramyxoviridae Infections - virology Phylogenetics Phylogeny Polymerase chain reaction Reverse transcription RNA, Viral - genetics Sequence Analysis, DNA Strains (organisms) Urine Veterinary Microbiology - Research Paper Viral Proteins - genetics Viruses |
| title | Molecular characterization of Feline paramyxovirus and Feline morbillivirus in cats from Brazil |
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