Serologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil

Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. To improve the information regarding flaviviruses in Southern Brazil, hor...

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Veröffentlicht in:Brazilian journal of microbiology 2021-06, Vol.52 (2), p.1021-1027
Hauptverfasser: Weber, Matheus N., Mosena, Ana C. S., Baumbach, Letícia F., da Silva, Mariana S., Canova, Raíssa, dos Santos, Débora R. L., Budaszewski, Renata da F., de Oliveira, Livia V., Soane, Michel M., Saraiva, Natália B., Bellucco, Fernanda T., Mazurek, Bruno Amaral, Diehl, Gustavo N., Gil, Laura H. V. G., Borba, Mauro R., Corbellini, Luis G., Canal, Cláudio W.
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container_end_page 1027
container_issue 2
container_start_page 1021
container_title Brazilian journal of microbiology
container_volume 52
creator Weber, Matheus N.
Mosena, Ana C. S.
Baumbach, Letícia F.
da Silva, Mariana S.
Canova, Raíssa
dos Santos, Débora R. L.
Budaszewski, Renata da F.
de Oliveira, Livia V.
Soane, Michel M.
Saraiva, Natália B.
Bellucco, Fernanda T.
Mazurek, Bruno Amaral
Diehl, Gustavo N.
Gil, Laura H. V. G.
Borba, Mauro R.
Corbellini, Luis G.
Canal, Cláudio W.
description Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. To improve the information regarding flaviviruses in Southern Brazil, horse serum samples were analyzed using RT-qPCR and a commercial ELISA-Ab against WNV followed by PRNT 75 . All 1000 samples analyzed by real-time RT-PCR resulted negative. The 465 subsampled samples were analyzed by a commercial ELISA-Ab against WNV, and the 18.5% (86/465) positive samples were further analyzed by PRNT 75 . In the PRNT 75 , 13/86 and 2/86 horses were positive for SLEV and WNV, respectively. It was observed that 5.8% (13/226) of the farms presented at least one positive animal for SLEV in PRNT 75 , whereas 0.9% (2/226) for WNV. Apart from the lower seroprevalences identified when compared to data previously reported in other Brazilian regions, our results suggest that public health professionals must be aware of the presence of these potential zoonotic pathogens.
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S. ; Baumbach, Letícia F. ; da Silva, Mariana S. ; Canova, Raíssa ; dos Santos, Débora R. L. ; Budaszewski, Renata da F. ; de Oliveira, Livia V. ; Soane, Michel M. ; Saraiva, Natália B. ; Bellucco, Fernanda T. ; Mazurek, Bruno Amaral ; Diehl, Gustavo N. ; Gil, Laura H. V. G. ; Borba, Mauro R. ; Corbellini, Luis G. ; Canal, Cláudio W.</creator><creatorcontrib>Weber, Matheus N. ; Mosena, Ana C. S. ; Baumbach, Letícia F. ; da Silva, Mariana S. ; Canova, Raíssa ; dos Santos, Débora R. L. ; Budaszewski, Renata da F. ; de Oliveira, Livia V. ; Soane, Michel M. ; Saraiva, Natália B. ; Bellucco, Fernanda T. ; Mazurek, Bruno Amaral ; Diehl, Gustavo N. ; Gil, Laura H. V. G. ; Borba, Mauro R. ; Corbellini, Luis G. ; Canal, Cláudio W.</creatorcontrib><description>Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. 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G.</creatorcontrib><creatorcontrib>Borba, Mauro R.</creatorcontrib><creatorcontrib>Corbellini, Luis G.</creatorcontrib><creatorcontrib>Canal, Cláudio W.</creatorcontrib><title>Serologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. To improve the information regarding flaviviruses in Southern Brazil, horse serum samples were analyzed using RT-qPCR and a commercial ELISA-Ab against WNV followed by PRNT 75 . All 1000 samples analyzed by real-time RT-PCR resulted negative. 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subjects Animals
Antibodies, Viral - blood
Biomedical and Life Sciences
Brazil - epidemiology
Encephalitis
Encephalitis Virus, St. Louis - genetics
Encephalitis Virus, St. Louis - immunology
Encephalitis Virus, St. Louis - isolation & purification
Encephalitis, Arbovirus - blood
Encephalitis, Arbovirus - epidemiology
Encephalitis, Arbovirus - veterinary
Encephalitis, Arbovirus - virology
Farms
Flavivirus Infections - blood
Flavivirus Infections - epidemiology
Flavivirus Infections - veterinary
Flavivirus Infections - virology
Food Microbiology
Geography
Horse Diseases - blood
Horse Diseases - epidemiology
Horse Diseases - virology
Horses
Life Sciences
Medical Microbiology
Medical personnel
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Mycology
Polymerase chain reaction
Public health
RNA, Viral - genetics
Seroepidemiologic Studies
Serology
Vector-borne diseases
Veterinary Microbiology - Research Paper
Viruses
West Nile virus
West Nile virus - genetics
West Nile virus - immunology
West Nile virus - isolation & purification
title Serologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil
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