Serologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil
Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. To improve the information regarding flaviviruses in Southern Brazil, hor...
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creator | Weber, Matheus N. Mosena, Ana C. S. Baumbach, Letícia F. da Silva, Mariana S. Canova, Raíssa dos Santos, Débora R. L. Budaszewski, Renata da F. de Oliveira, Livia V. Soane, Michel M. Saraiva, Natália B. Bellucco, Fernanda T. Mazurek, Bruno Amaral Diehl, Gustavo N. Gil, Laura H. V. G. Borba, Mauro R. Corbellini, Luis G. Canal, Cláudio W. |
description | Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. To improve the information regarding flaviviruses in Southern Brazil, horse serum samples were analyzed using RT-qPCR and a commercial ELISA-Ab against WNV followed by PRNT
75
. All 1000 samples analyzed by real-time RT-PCR resulted negative. The 465 subsampled samples were analyzed by a commercial ELISA-Ab against WNV, and the 18.5% (86/465) positive samples were further analyzed by PRNT
75
. In the PRNT
75
, 13/86 and 2/86 horses were positive for SLEV and WNV, respectively. It was observed that 5.8% (13/226) of the farms presented at least one positive animal for SLEV in PRNT
75
, whereas 0.9% (2/226) for WNV. Apart from the lower seroprevalences identified when compared to data previously reported in other Brazilian regions, our results suggest that public health professionals must be aware of the presence of these potential zoonotic pathogens. |
doi_str_mv | 10.1007/s42770-021-00474-7 |
format | Article |
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75
. All 1000 samples analyzed by real-time RT-PCR resulted negative. The 465 subsampled samples were analyzed by a commercial ELISA-Ab against WNV, and the 18.5% (86/465) positive samples were further analyzed by PRNT
75
. In the PRNT
75
, 13/86 and 2/86 horses were positive for SLEV and WNV, respectively. It was observed that 5.8% (13/226) of the farms presented at least one positive animal for SLEV in PRNT
75
, whereas 0.9% (2/226) for WNV. Apart from the lower seroprevalences identified when compared to data previously reported in other Brazilian regions, our results suggest that public health professionals must be aware of the presence of these potential zoonotic pathogens.</description><identifier>ISSN: 1517-8382</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-021-00474-7</identifier><identifier>PMID: 33797731</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animals ; Antibodies, Viral - blood ; Biomedical and Life Sciences ; Brazil - epidemiology ; Encephalitis ; Encephalitis Virus, St. Louis - genetics ; Encephalitis Virus, St. Louis - immunology ; Encephalitis Virus, St. Louis - isolation & purification ; Encephalitis, Arbovirus - blood ; Encephalitis, Arbovirus - epidemiology ; Encephalitis, Arbovirus - veterinary ; Encephalitis, Arbovirus - virology ; Farms ; Flavivirus Infections - blood ; Flavivirus Infections - epidemiology ; Flavivirus Infections - veterinary ; Flavivirus Infections - virology ; Food Microbiology ; Geography ; Horse Diseases - blood ; Horse Diseases - epidemiology ; Horse Diseases - virology ; Horses ; Life Sciences ; Medical Microbiology ; Medical personnel ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Mycology ; Polymerase chain reaction ; Public health ; RNA, Viral - genetics ; Seroepidemiologic Studies ; Serology ; Vector-borne diseases ; Veterinary Microbiology - Research Paper ; Viruses ; West Nile virus ; West Nile virus - genetics ; West Nile virus - immunology ; West Nile virus - isolation & purification</subject><ispartof>Brazilian journal of microbiology, 2021-06, Vol.52 (2), p.1021-1027</ispartof><rights>Sociedade Brasileira de Microbiologia 2021</rights><rights>Sociedade Brasileira de Microbiologia 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-115484684bb4b01834d4020e5aacba694496e9409435d193eef582c615a3f2523</citedby><cites>FETCH-LOGICAL-c474t-115484684bb4b01834d4020e5aacba694496e9409435d193eef582c615a3f2523</cites><orcidid>0000-0001-8282-6778</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105465/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105465/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,41467,42536,51297,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33797731$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weber, Matheus N.</creatorcontrib><creatorcontrib>Mosena, Ana C. S.</creatorcontrib><creatorcontrib>Baumbach, Letícia F.</creatorcontrib><creatorcontrib>da Silva, Mariana S.</creatorcontrib><creatorcontrib>Canova, Raíssa</creatorcontrib><creatorcontrib>dos Santos, Débora R. L.</creatorcontrib><creatorcontrib>Budaszewski, Renata da F.</creatorcontrib><creatorcontrib>de Oliveira, Livia V.</creatorcontrib><creatorcontrib>Soane, Michel M.</creatorcontrib><creatorcontrib>Saraiva, Natália B.</creatorcontrib><creatorcontrib>Bellucco, Fernanda T.</creatorcontrib><creatorcontrib>Mazurek, Bruno Amaral</creatorcontrib><creatorcontrib>Diehl, Gustavo N.</creatorcontrib><creatorcontrib>Gil, Laura H. V. G.</creatorcontrib><creatorcontrib>Borba, Mauro R.</creatorcontrib><creatorcontrib>Corbellini, Luis G.</creatorcontrib><creatorcontrib>Canal, Cláudio W.</creatorcontrib><title>Serologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. To improve the information regarding flaviviruses in Southern Brazil, horse serum samples were analyzed using RT-qPCR and a commercial ELISA-Ab against WNV followed by PRNT
75
. All 1000 samples analyzed by real-time RT-PCR resulted negative. The 465 subsampled samples were analyzed by a commercial ELISA-Ab against WNV, and the 18.5% (86/465) positive samples were further analyzed by PRNT
75
. In the PRNT
75
, 13/86 and 2/86 horses were positive for SLEV and WNV, respectively. It was observed that 5.8% (13/226) of the farms presented at least one positive animal for SLEV in PRNT
75
, whereas 0.9% (2/226) for WNV. Apart from the lower seroprevalences identified when compared to data previously reported in other Brazilian regions, our results suggest that public health professionals must be aware of the presence of these potential zoonotic pathogens.</description><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Biomedical and Life Sciences</subject><subject>Brazil - epidemiology</subject><subject>Encephalitis</subject><subject>Encephalitis Virus, St. Louis - genetics</subject><subject>Encephalitis Virus, St. Louis - immunology</subject><subject>Encephalitis Virus, St. Louis - isolation & purification</subject><subject>Encephalitis, Arbovirus - blood</subject><subject>Encephalitis, Arbovirus - epidemiology</subject><subject>Encephalitis, Arbovirus - veterinary</subject><subject>Encephalitis, Arbovirus - virology</subject><subject>Farms</subject><subject>Flavivirus Infections - blood</subject><subject>Flavivirus Infections - epidemiology</subject><subject>Flavivirus Infections - veterinary</subject><subject>Flavivirus Infections - virology</subject><subject>Food Microbiology</subject><subject>Geography</subject><subject>Horse Diseases - blood</subject><subject>Horse Diseases - epidemiology</subject><subject>Horse Diseases - virology</subject><subject>Horses</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Medical personnel</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Polymerase chain reaction</subject><subject>Public health</subject><subject>RNA, Viral - genetics</subject><subject>Seroepidemiologic Studies</subject><subject>Serology</subject><subject>Vector-borne diseases</subject><subject>Veterinary Microbiology - Research Paper</subject><subject>Viruses</subject><subject>West Nile virus</subject><subject>West Nile virus - genetics</subject><subject>West Nile virus - immunology</subject><subject>West Nile virus - isolation & purification</subject><issn>1517-8382</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EoqXwBzggS1y4hPpjHDsXJFrxJa3gsCCOlpNMdl1l7cVOVoJfj7cppXDoybbmmdfzzkvIc85ec8b0eQahNauY4BVjoKHSD8gpr7WpAJh6WO6K68pII07Ik5yvGBOKgXhMTqTUjdaSn5LNGlMc48Z3FA--x9AhjQP9jnmin_2I9ODTnKkLPV07Hya6irPP9Mjtt270U3ksiA90G1PGTIcUd3Qd52mLKdCL5H758Sl5NLgx47Ob84x8e__u6-XHavXlw6fLt6uqKwaminMFBmoDbQst40ZCD0wwVM51rasbgKbGBlgDUvW8kYiDMqKruXJyEErIM_Jm0d3P7Q77DsOU3Gj3ye9c-mmj8_bfSvBbu4kHazhTUKsi8OpGIMUfc1mD3fnc4Ti6gHHOtqzQqAI3sqAv_0Ov4pxCsWePo3DgAEdBsVBdijknHG6H4cwec7RLjrbkaK9ztLo0vbhr47blT3AFkAuQSylsMP39-x7Z3_iIqL8</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Weber, Matheus N.</creator><creator>Mosena, Ana C. S.</creator><creator>Baumbach, Letícia F.</creator><creator>da Silva, Mariana S.</creator><creator>Canova, Raíssa</creator><creator>dos Santos, Débora R. L.</creator><creator>Budaszewski, Renata da F.</creator><creator>de Oliveira, Livia V.</creator><creator>Soane, Michel M.</creator><creator>Saraiva, Natália B.</creator><creator>Bellucco, Fernanda T.</creator><creator>Mazurek, Bruno Amaral</creator><creator>Diehl, Gustavo N.</creator><creator>Gil, Laura H. V. G.</creator><creator>Borba, Mauro R.</creator><creator>Corbellini, Luis G.</creator><creator>Canal, Cláudio W.</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8282-6778</orcidid></search><sort><creationdate>20210601</creationdate><title>Serologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil</title><author>Weber, Matheus N. ; Mosena, Ana C. S. ; Baumbach, Letícia F. ; da Silva, Mariana S. ; Canova, Raíssa ; dos Santos, Débora R. L. ; Budaszewski, Renata da F. ; de Oliveira, Livia V. ; Soane, Michel M. ; Saraiva, Natália B. ; Bellucco, Fernanda T. ; Mazurek, Bruno Amaral ; Diehl, Gustavo N. ; Gil, Laura H. V. G. ; Borba, Mauro R. ; Corbellini, Luis G. ; Canal, Cláudio W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-115484684bb4b01834d4020e5aacba694496e9409435d193eef582c615a3f2523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Biomedical and Life Sciences</topic><topic>Brazil - epidemiology</topic><topic>Encephalitis</topic><topic>Encephalitis Virus, St. Louis - genetics</topic><topic>Encephalitis Virus, St. Louis - immunology</topic><topic>Encephalitis Virus, St. Louis - isolation & purification</topic><topic>Encephalitis, Arbovirus - blood</topic><topic>Encephalitis, Arbovirus - epidemiology</topic><topic>Encephalitis, Arbovirus - veterinary</topic><topic>Encephalitis, Arbovirus - virology</topic><topic>Farms</topic><topic>Flavivirus Infections - blood</topic><topic>Flavivirus Infections - epidemiology</topic><topic>Flavivirus Infections - veterinary</topic><topic>Flavivirus Infections - virology</topic><topic>Food Microbiology</topic><topic>Geography</topic><topic>Horse Diseases - blood</topic><topic>Horse Diseases - epidemiology</topic><topic>Horse Diseases - virology</topic><topic>Horses</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Medical personnel</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>Polymerase chain reaction</topic><topic>Public health</topic><topic>RNA, Viral - genetics</topic><topic>Seroepidemiologic Studies</topic><topic>Serology</topic><topic>Vector-borne diseases</topic><topic>Veterinary Microbiology - Research Paper</topic><topic>Viruses</topic><topic>West Nile virus</topic><topic>West Nile virus - genetics</topic><topic>West Nile virus - immunology</topic><topic>West Nile virus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weber, Matheus N.</creatorcontrib><creatorcontrib>Mosena, Ana C. S.</creatorcontrib><creatorcontrib>Baumbach, Letícia F.</creatorcontrib><creatorcontrib>da Silva, Mariana S.</creatorcontrib><creatorcontrib>Canova, Raíssa</creatorcontrib><creatorcontrib>dos Santos, Débora R. L.</creatorcontrib><creatorcontrib>Budaszewski, Renata da F.</creatorcontrib><creatorcontrib>de Oliveira, Livia V.</creatorcontrib><creatorcontrib>Soane, Michel M.</creatorcontrib><creatorcontrib>Saraiva, Natália B.</creatorcontrib><creatorcontrib>Bellucco, Fernanda T.</creatorcontrib><creatorcontrib>Mazurek, Bruno Amaral</creatorcontrib><creatorcontrib>Diehl, Gustavo N.</creatorcontrib><creatorcontrib>Gil, Laura H. V. G.</creatorcontrib><creatorcontrib>Borba, Mauro R.</creatorcontrib><creatorcontrib>Corbellini, Luis G.</creatorcontrib><creatorcontrib>Canal, Cláudio W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weber, Matheus N.</au><au>Mosena, Ana C. S.</au><au>Baumbach, Letícia F.</au><au>da Silva, Mariana S.</au><au>Canova, Raíssa</au><au>dos Santos, Débora R. L.</au><au>Budaszewski, Renata da F.</au><au>de Oliveira, Livia V.</au><au>Soane, Michel M.</au><au>Saraiva, Natália B.</au><au>Bellucco, Fernanda T.</au><au>Mazurek, Bruno Amaral</au><au>Diehl, Gustavo N.</au><au>Gil, Laura H. V. G.</au><au>Borba, Mauro R.</au><au>Corbellini, Luis G.</au><au>Canal, Cláudio W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil</atitle><jtitle>Brazilian journal of microbiology</jtitle><stitle>Braz J Microbiol</stitle><addtitle>Braz J Microbiol</addtitle><date>2021-06-01</date><risdate>2021</risdate><volume>52</volume><issue>2</issue><spage>1021</spage><epage>1027</epage><pages>1021-1027</pages><issn>1517-8382</issn><eissn>1678-4405</eissn><abstract>Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. To improve the information regarding flaviviruses in Southern Brazil, horse serum samples were analyzed using RT-qPCR and a commercial ELISA-Ab against WNV followed by PRNT
75
. All 1000 samples analyzed by real-time RT-PCR resulted negative. The 465 subsampled samples were analyzed by a commercial ELISA-Ab against WNV, and the 18.5% (86/465) positive samples were further analyzed by PRNT
75
. In the PRNT
75
, 13/86 and 2/86 horses were positive for SLEV and WNV, respectively. It was observed that 5.8% (13/226) of the farms presented at least one positive animal for SLEV in PRNT
75
, whereas 0.9% (2/226) for WNV. Apart from the lower seroprevalences identified when compared to data previously reported in other Brazilian regions, our results suggest that public health professionals must be aware of the presence of these potential zoonotic pathogens.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>33797731</pmid><doi>10.1007/s42770-021-00474-7</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-8282-6778</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Animals Antibodies, Viral - blood Biomedical and Life Sciences Brazil - epidemiology Encephalitis Encephalitis Virus, St. Louis - genetics Encephalitis Virus, St. Louis - immunology Encephalitis Virus, St. Louis - isolation & purification Encephalitis, Arbovirus - blood Encephalitis, Arbovirus - epidemiology Encephalitis, Arbovirus - veterinary Encephalitis, Arbovirus - virology Farms Flavivirus Infections - blood Flavivirus Infections - epidemiology Flavivirus Infections - veterinary Flavivirus Infections - virology Food Microbiology Geography Horse Diseases - blood Horse Diseases - epidemiology Horse Diseases - virology Horses Life Sciences Medical Microbiology Medical personnel Microbial Ecology Microbial Genetics and Genomics Microbiology Mycology Polymerase chain reaction Public health RNA, Viral - genetics Seroepidemiologic Studies Serology Vector-borne diseases Veterinary Microbiology - Research Paper Viruses West Nile virus West Nile virus - genetics West Nile virus - immunology West Nile virus - isolation & purification |
title | Serologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil |
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