Clinical impact of panel-based error-corrected next generation sequencing versus flow cytometry to detect measurable residual disease (MRD) in acute myeloid leukemia (AML)

We accrued 201 patients of adult AML treated with conventional therapy, in morphological remission, and evaluated MRD using sensitive error-corrected next generation sequencing (NGS-MRD) and multiparameter flow cytometry (FCM-MRD) at the end of induction (PI) and consolidation (PC). Nearly 71% of pa...

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Veröffentlicht in:	Leukemia 2021-05, Vol.35 (5), p.1392-1404
Hauptverfasser:	Patkar, Nikhil, Kakirde, Chinmayee, Shaikh, Anam Fatima, Salve, Rakhi, Bhanshe, Prasanna, Chatterjee, Gaurav, Rajpal, Sweta, Joshi, Swapnali, Chaudhary, Shruti, Kodgule, Rohan, Ghoghale, Sitaram, Deshpande, Nilesh, Shetty, Dhanalaxmi, Khizer, Syed Hasan, Jain, Hasmukh, Bagal, Bhausaheb, Menon, Hari, Khattry, Navin, Sengar, Manju, Tembhare, Prashant, Subramanian, Papagudi, Gujral, Sumeet
Format:	Artikel
Sprache:	eng
Schlagworte:	45 631/67/69 692/308/575 Acute myeloid leukemia Adolescent Adult Cancer cells Cancer Research Comparative analysis Critical Care Medicine Cytogenetics Disease Progression DNA sequencing Error correction Female Flow cytometry Flow Cytometry - methods Genetic aspects Hematology Hematopoietic Stem Cell Transplantation - methods High-Throughput Nucleotide Sequencing - methods Humans Identification and classification Intensive Internal Medicine Leukemia Leukemia, Myeloid, Acute - genetics Leukemia, Myeloid, Acute - pathology Male Measurement Medicine Medicine & Public Health Middle Aged Mutation - genetics Myeloid leukemia Neoplasm, Residual - diagnosis Neoplasm, Residual - genetics Neoplasm, Residual - pathology Next-generation sequencing Nucleotide sequencing Oncology Recurrence Remission Risk Sensitivity analysis Survival Young Adult
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Zusammenfassung:	We accrued 201 patients of adult AML treated with conventional therapy, in morphological remission, and evaluated MRD using sensitive error-corrected next generation sequencing (NGS-MRD) and multiparameter flow cytometry (FCM-MRD) at the end of induction (PI) and consolidation (PC). Nearly 71% of patients were PI NGS-MRD + and 40.9% PC NGS-MRD + (median VAF 0.76%). NGS-MRD + patients had a significantly higher cumulative incidence of relapse ( p = 0.003), inferior overall survival ( p = 0.001) and relapse free survival ( p
ISSN:	0887-6924 1476-5551
DOI:	10.1038/s41375-021-01131-6