High-Yield Preparation of Outer Membrane Protein Efflux Pumps by in Vitro Refolding is Concentration Dependent

Overexpression of tripartite efflux pump systems in gram-negative bacteria is a principal component of antibiotic resistance. High-yield purification of the outer membrane component of these systems will enable biochemical and structural interrogation of their mechanisms of action and allow testing...

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Veröffentlicht in:	The Journal of membrane biology 2021-02, Vol.254 (1), p.41-50
Hauptverfasser:	Budiardjo, S. Jimmy, Ikujuni, Ayotunde Paul, Firlar, Emre, Cordova, Andrés, Kaelber, Jason T., Slusky, Joanna S. G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotic resistance Antibiotics Bacterial Outer Membrane Proteins - metabolism Biochemistry Biological Transport Biomedical and Life Sciences BLANCO-80: Thematic Collection of Structural and Thermodynamic Studies on Membranes Crystal structure Efflux Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Gel electrophoresis Gram-Negative Bacteria Homology Human Physiology Inclusion bodies Interrogation Life Sciences Lipids Membrane proteins Membrane Proteins - metabolism Membranes Micelles Oligomerization Outer membrane proteins Protein folding Proteins Sodium lauryl sulfate Urea
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