Transcriptome Analysis of Listeria monocytogenes Exposed to Beef Fat Reveals Antimicrobial and Pathogenicity Attenuation Mechanisms

is a deadly intracellular pathogen mostly associated with consumption of ready-to-eat foods. This study investigated the effectiveness of total beef fat (BF-T) from flaxseed-fed cattle and its fractions enriched with monounsaturated fatty acids (BF-MUFA) and polyunsaturated fatty acids (BF-PUFA), al...

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Veröffentlicht in:	Applied and environmental microbiology 2021-04, Vol.87 (9), p.1
Hauptverfasser:	Chen, Yuan Yao, Kommadath, Arun, Vahmani, Payam, Visvalingam, Jeyachchandran, Dugan, Michael E R, Yang, Xianqin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal fat Animals Anti-Bacterial Agents - pharmacology Antiinfectives and antibacterials Antimicrobial agents Attenuation Beef Beef cattle Bile Cattle Fats - pharmacology Fatty acids Fatty Acids - pharmacology Food Food consumption Food Microbiology Food processing Foodborne pathogens Functional analysis Gene expression Gene Expression Profiling Gene Expression Regulation, Bacterial - drug effects Gene sequencing Genes Listeria Listeria monocytogenes Listeria monocytogenes - drug effects Listeria monocytogenes - genetics Listeria monocytogenes - growth & development Minimum inhibitory concentration Mode of action Nutrient transport Oxidation resistance Oxidative stress Pathogenicity Pathogens Polyunsaturated fatty acids Red Meat Sigma factor Transcription Transcriptomes Virulence
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Zusammenfassung:	is a deadly intracellular pathogen mostly associated with consumption of ready-to-eat foods. This study investigated the effectiveness of total beef fat (BF-T) from flaxseed-fed cattle and its fractions enriched with monounsaturated fatty acids (BF-MUFA) and polyunsaturated fatty acids (BF-PUFA), along with commercially available long-chain fatty acids (LC-FA), as natural antimicrobials against BF-T was ineffective at concentrations up to 6 mg/ml, while was susceptible to BF-MUFA and BF-PUFA, with MICs at pH 7 of 0.33 ± 0.21 mg/ml and 0.06 ± 0.03 mg/ml, respectively. The MIC of C14:0 was significantly lower than those of C16:0 and C18:0 ( 0.05). Fatty acids 9-C16:1, C18:2n-6, and C18:3n-3 showed stronger inhibitory activity than 9-C18:1 and conjugated C18:2, with MICs of
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.03027-20