Target protein deglycosylation in living cells by a nanobody-fused split O-GlcNAcase

O-linked N -acetylglucosamine ( O -GlcNAc) is an essential and dynamic post-translational modification that is presented on thousands of nucleocytoplasmic proteins. Interrogating the role of O -GlcNAc on a single target protein is crucial, yet challenging to perform in cells. Herein, we developed a...

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Veröffentlicht in:	Nature chemical biology 2021-05, Vol.17 (5), p.593-600
Hauptverfasser:	Ge, Yun, Ramirez, Daniel H., Yang, Bo, D’Souza, Alexandria K., Aonbangkhen, Chanat, Wong, Stephanie, Woo, Christina M.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/92/221 631/92/458 631/92/96 Antigens, Neoplasm - chemistry Antigens, Neoplasm - genetics Antigens, Neoplasm - metabolism Biochemical Engineering Biochemistry Biological Assay Bioorganic Chemistry c-Fos protein c-Jun protein Catalytic Domain Cell Biology Cell fusion Chemistry Chemistry and Materials Science Chemistry/Food Science Deglycosylation Drug Delivery Systems - methods Gene Expression Glycosylation HEK293 Cells Histone Acetyltransferases - chemistry Histone Acetyltransferases - genetics Histone Acetyltransferases - metabolism Humans Hyaluronoglucosaminidase - chemistry Hyaluronoglucosaminidase - genetics Hyaluronoglucosaminidase - metabolism Hydrolysis JNK Mitogen-Activated Protein Kinases - genetics JNK Mitogen-Activated Protein Kinases - metabolism Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism N-Acetylglucosamine Nanobodies Nuclear Pore Complex Proteins - genetics Nuclear Pore Complex Proteins - metabolism Optimization Plasmids - chemistry Plasmids - metabolism Post-translation Protein Binding Proteins Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Single-Domain Antibodies - chemistry Single-Domain Antibodies - metabolism Sp1 Transcription Factor - genetics Sp1 Transcription Factor - metabolism Transcription factors Transcription Factors - genetics Transcription Factors - metabolism Transfection - methods
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