DEAD-box helicases modulate dicing body formation in Arabidopsis
Eukaryotic cells contain numerous membraneless organelles that are made from liquid droplets of proteins and nucleic acids and that provide spatiotemporal control of various cellular processes. However, the molecular mechanisms underlying the formation and rapid stress-induced alterations of these o...
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| Veröffentlicht in: | Science advances 2021-04, Vol.7 (18) |
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| creator | Li, Qi Liu, Ningkun Liu, Qing Zheng, Xingguo Lu, Lu Gao, Wenrui Liu, Yang Liu, Yan Zhang, Shicheng Wang, Qian Pan, Jing Chen, Chen Mi, Yingjie Yang, Meiling Cheng, Xiaofei Ren, Guodong Yuan, Yao-Wu Zhang, Xiaoming |
| description | Eukaryotic cells contain numerous membraneless organelles that are made from liquid droplets of proteins and nucleic acids and that provide spatiotemporal control of various cellular processes. However, the molecular mechanisms underlying the formation and rapid stress-induced alterations of these organelles are relatively uncharacterized. Here, we investigated the roles of DEAD-box helicases in the formation and alteration of membraneless nuclear dicing bodies (D-bodies) in
We uncovered that RNA helicase 6 (RH6), RH8, and RH12 are previously unidentified D-body components. These helicases interact with and promote the phase separation of SERRATE, a key component of D-bodies, and drive the formation of D-bodies through liquid-liquid phase separations (LLPSs). The accumulation of these helicases in the nuclei decreases upon
infections, which couples with the decrease of D-bodies. Our results thus reveal the key roles of RH6, RH8, and RH12 in modulating D-body formation via LLPSs. |
| doi_str_mv | 10.1126/sciadv.abc6266 |
| format | Article |
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We uncovered that RNA helicase 6 (RH6), RH8, and RH12 are previously unidentified D-body components. These helicases interact with and promote the phase separation of SERRATE, a key component of D-bodies, and drive the formation of D-bodies through liquid-liquid phase separations (LLPSs). The accumulation of these helicases in the nuclei decreases upon
infections, which couples with the decrease of D-bodies. Our results thus reveal the key roles of RH6, RH8, and RH12 in modulating D-body formation via LLPSs.</description><identifier>ISSN: 2375-2548</identifier><identifier>EISSN: 2375-2548</identifier><identifier>DOI: 10.1126/sciadv.abc6266</identifier><identifier>PMID: 33910901</identifier><language>eng</language><publisher>United States: American Association for the Advancement of Science</publisher><subject>Arabidopsis - genetics ; Arabidopsis - metabolism ; Biochemistry ; Cell Nucleus - metabolism ; DEAD-box RNA Helicases - genetics ; DEAD-box RNA Helicases - metabolism ; DNA Helicases - metabolism ; Plant Sciences ; SciAdv r-articles</subject><ispartof>Science advances, 2021-04, Vol.7 (18)</ispartof><rights>Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).</rights><rights>Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 2021 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-344007734b77b9f87534511ab891d3ba0de26f4021ecd41ac092e83f4b0ab7c23</citedby><cites>FETCH-LOGICAL-c390t-344007734b77b9f87534511ab891d3ba0de26f4021ecd41ac092e83f4b0ab7c23</cites><orcidid>0000-0003-1376-0028 ; 0000-0002-3190-4163 ; 0000-0003-2153-5242 ; 0000-0003-3678-0654 ; 0000-0002-9771-9059 ; 0000-0002-0033-0400 ; 0000-0002-1895-7578 ; 0000-0003-1187-6780</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081359/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081359/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33910901$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Qi</creatorcontrib><creatorcontrib>Liu, Ningkun</creatorcontrib><creatorcontrib>Liu, Qing</creatorcontrib><creatorcontrib>Zheng, Xingguo</creatorcontrib><creatorcontrib>Lu, Lu</creatorcontrib><creatorcontrib>Gao, Wenrui</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Zhang, Shicheng</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Pan, Jing</creatorcontrib><creatorcontrib>Chen, Chen</creatorcontrib><creatorcontrib>Mi, Yingjie</creatorcontrib><creatorcontrib>Yang, Meiling</creatorcontrib><creatorcontrib>Cheng, Xiaofei</creatorcontrib><creatorcontrib>Ren, Guodong</creatorcontrib><creatorcontrib>Yuan, Yao-Wu</creatorcontrib><creatorcontrib>Zhang, Xiaoming</creatorcontrib><title>DEAD-box helicases modulate dicing body formation in Arabidopsis</title><title>Science advances</title><addtitle>Sci Adv</addtitle><description>Eukaryotic cells contain numerous membraneless organelles that are made from liquid droplets of proteins and nucleic acids and that provide spatiotemporal control of various cellular processes. However, the molecular mechanisms underlying the formation and rapid stress-induced alterations of these organelles are relatively uncharacterized. Here, we investigated the roles of DEAD-box helicases in the formation and alteration of membraneless nuclear dicing bodies (D-bodies) in
We uncovered that RNA helicase 6 (RH6), RH8, and RH12 are previously unidentified D-body components. These helicases interact with and promote the phase separation of SERRATE, a key component of D-bodies, and drive the formation of D-bodies through liquid-liquid phase separations (LLPSs). The accumulation of these helicases in the nuclei decreases upon
infections, which couples with the decrease of D-bodies. Our results thus reveal the key roles of RH6, RH8, and RH12 in modulating D-body formation via LLPSs.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Biochemistry</subject><subject>Cell Nucleus - metabolism</subject><subject>DEAD-box RNA Helicases - genetics</subject><subject>DEAD-box RNA Helicases - metabolism</subject><subject>DNA Helicases - metabolism</subject><subject>Plant Sciences</subject><subject>SciAdv r-articles</subject><issn>2375-2548</issn><issn>2375-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkMtLAzEQh4MottRePcoevWzNazfJRSxtfUDBi55DXttGdjc12Rb737vSWvQ0A_PNb4YPgGsEJwjh8i4Zr-xuorQpcVmegSEmrMhxQfn5n34Axil9QAgRLcsCiUswIEQgKCAagof5YjrPdfjK1q72RiWXsibYba06l1lvfLvKdLD7rAqxUZ0PbebbbBqV9jZskk9X4KJSdXLjYx2B98fF2-w5X74-vcymy9wQAbucUAohY4RqxrSoOCsILRBSmgtkiVbQOlxWFGLkjKVIGSiw46SiGirNDCYjcH_I3Wx146xxbRdVLTfRNyruZVBe_p-0fi1XYSc55IgUog-4PQbE8Ll1qZONT8bVtWpd2CaJezUcIcZ4j04OqIkhpeiq0xkE5Y95eTAvj-b7hZu_z53wX8_kG9oHgPU</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Li, Qi</creator><creator>Liu, Ningkun</creator><creator>Liu, Qing</creator><creator>Zheng, Xingguo</creator><creator>Lu, Lu</creator><creator>Gao, Wenrui</creator><creator>Liu, Yang</creator><creator>Liu, Yan</creator><creator>Zhang, Shicheng</creator><creator>Wang, Qian</creator><creator>Pan, Jing</creator><creator>Chen, Chen</creator><creator>Mi, Yingjie</creator><creator>Yang, Meiling</creator><creator>Cheng, Xiaofei</creator><creator>Ren, Guodong</creator><creator>Yuan, Yao-Wu</creator><creator>Zhang, Xiaoming</creator><general>American Association for the Advancement of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1376-0028</orcidid><orcidid>https://orcid.org/0000-0002-3190-4163</orcidid><orcidid>https://orcid.org/0000-0003-2153-5242</orcidid><orcidid>https://orcid.org/0000-0003-3678-0654</orcidid><orcidid>https://orcid.org/0000-0002-9771-9059</orcidid><orcidid>https://orcid.org/0000-0002-0033-0400</orcidid><orcidid>https://orcid.org/0000-0002-1895-7578</orcidid><orcidid>https://orcid.org/0000-0003-1187-6780</orcidid></search><sort><creationdate>20210401</creationdate><title>DEAD-box helicases modulate dicing body formation in Arabidopsis</title><author>Li, Qi ; Liu, Ningkun ; Liu, Qing ; Zheng, Xingguo ; Lu, Lu ; Gao, Wenrui ; Liu, Yang ; Liu, Yan ; Zhang, Shicheng ; Wang, Qian ; Pan, Jing ; Chen, Chen ; Mi, Yingjie ; Yang, Meiling ; Cheng, Xiaofei ; Ren, Guodong ; Yuan, Yao-Wu ; Zhang, Xiaoming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-344007734b77b9f87534511ab891d3ba0de26f4021ecd41ac092e83f4b0ab7c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Biochemistry</topic><topic>Cell Nucleus - metabolism</topic><topic>DEAD-box RNA Helicases - genetics</topic><topic>DEAD-box RNA Helicases - metabolism</topic><topic>DNA Helicases - metabolism</topic><topic>Plant Sciences</topic><topic>SciAdv r-articles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Qi</creatorcontrib><creatorcontrib>Liu, Ningkun</creatorcontrib><creatorcontrib>Liu, Qing</creatorcontrib><creatorcontrib>Zheng, Xingguo</creatorcontrib><creatorcontrib>Lu, Lu</creatorcontrib><creatorcontrib>Gao, Wenrui</creatorcontrib><creatorcontrib>Liu, Yang</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Zhang, Shicheng</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Pan, Jing</creatorcontrib><creatorcontrib>Chen, Chen</creatorcontrib><creatorcontrib>Mi, Yingjie</creatorcontrib><creatorcontrib>Yang, Meiling</creatorcontrib><creatorcontrib>Cheng, Xiaofei</creatorcontrib><creatorcontrib>Ren, Guodong</creatorcontrib><creatorcontrib>Yuan, Yao-Wu</creatorcontrib><creatorcontrib>Zhang, Xiaoming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Science advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Qi</au><au>Liu, Ningkun</au><au>Liu, Qing</au><au>Zheng, Xingguo</au><au>Lu, Lu</au><au>Gao, Wenrui</au><au>Liu, Yang</au><au>Liu, Yan</au><au>Zhang, Shicheng</au><au>Wang, Qian</au><au>Pan, Jing</au><au>Chen, Chen</au><au>Mi, Yingjie</au><au>Yang, Meiling</au><au>Cheng, Xiaofei</au><au>Ren, Guodong</au><au>Yuan, Yao-Wu</au><au>Zhang, Xiaoming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DEAD-box helicases modulate dicing body formation in Arabidopsis</atitle><jtitle>Science advances</jtitle><addtitle>Sci Adv</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>7</volume><issue>18</issue><issn>2375-2548</issn><eissn>2375-2548</eissn><abstract>Eukaryotic cells contain numerous membraneless organelles that are made from liquid droplets of proteins and nucleic acids and that provide spatiotemporal control of various cellular processes. However, the molecular mechanisms underlying the formation and rapid stress-induced alterations of these organelles are relatively uncharacterized. Here, we investigated the roles of DEAD-box helicases in the formation and alteration of membraneless nuclear dicing bodies (D-bodies) in
We uncovered that RNA helicase 6 (RH6), RH8, and RH12 are previously unidentified D-body components. These helicases interact with and promote the phase separation of SERRATE, a key component of D-bodies, and drive the formation of D-bodies through liquid-liquid phase separations (LLPSs). The accumulation of these helicases in the nuclei decreases upon
infections, which couples with the decrease of D-bodies. Our results thus reveal the key roles of RH6, RH8, and RH12 in modulating D-body formation via LLPSs.</abstract><cop>United States</cop><pub>American Association for the Advancement of Science</pub><pmid>33910901</pmid><doi>10.1126/sciadv.abc6266</doi><orcidid>https://orcid.org/0000-0003-1376-0028</orcidid><orcidid>https://orcid.org/0000-0002-3190-4163</orcidid><orcidid>https://orcid.org/0000-0003-2153-5242</orcidid><orcidid>https://orcid.org/0000-0003-3678-0654</orcidid><orcidid>https://orcid.org/0000-0002-9771-9059</orcidid><orcidid>https://orcid.org/0000-0002-0033-0400</orcidid><orcidid>https://orcid.org/0000-0002-1895-7578</orcidid><orcidid>https://orcid.org/0000-0003-1187-6780</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Arabidopsis - genetics Arabidopsis - metabolism Biochemistry Cell Nucleus - metabolism DEAD-box RNA Helicases - genetics DEAD-box RNA Helicases - metabolism DNA Helicases - metabolism Plant Sciences SciAdv r-articles |
| title | DEAD-box helicases modulate dicing body formation in Arabidopsis |
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