In Vitro α-Amylase and α-Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Hagenia abyssinica Leaves
Background. The leaves of Hagenia abyssinica have been used in the management of diabetes mellitus in Ethiopian folk medicine. Thus, this study is aimed at investigating the in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the crude extract and solvent fractions of H. ab...
Gespeichert in:
| Veröffentlicht in: | BioMed research international 2021, Vol.2021 (1), p.6652777-6652777 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 6652777 |
|---|---|
| container_issue | 1 |
| container_start_page | 6652777 |
| container_title | BioMed research international |
| container_volume | 2021 |
| creator | Kifle, Zemene Demelash Debeb, Simachew Gidey Belayneh, Yaschilal Muche |
| description | Background. The leaves of Hagenia abyssinica have been used in the management of diabetes mellitus in Ethiopian folk medicine. Thus, this study is aimed at investigating the in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the crude extract and solvent fractions of H. abyssinica leaves. Methods. The in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the plant extract were assessed using 3,5-dinitrosalicylic acid (DNSA), p-nitro-phenyl-a-D glucopyranoside (p-NPG), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays, respectively. Each value of percent inhibition of α-amylase, α-glucosidase, and DPPH scavenging effect was presented as means±SEM (n=3). Results. The α-amylase inhibitory activity of the crude extract and solvent fractions was found to be concentration-dependent. The strongest activity was exhibited by the crude extract at the highest concentration with a percentage inhibition of 74.52% (IC50, 14.52 μg/ml) followed by water fraction 68.24% (IC50, 16.31 μg/ml), ethyl acetate fraction 61.57% (IC50, 18.73 μg/ml), and chloroform fraction 56.87% (IC50, 21.57 μg/ml) of H. abyssinica leaves. In the α-glucosidase inhibition assay, the maximum activity was exhibited by the aqueous fraction 62.54% (IC50, 11.67 μg/ml) followed by ethyl acetate fraction 54.97% (IC50, 15.89 μg/ml), crude extract 46.79% (IC50, >16.5 μg/ml), and chloroform fraction 36.44% (IC50, >16.5 μg/ml). In the antioxidant assay, the crude extract exhibited the highest antioxidant activity 86.36% (IC50, 10.25 μg/ml) followed by water fraction 78.59% (IC50, 13.86 μg/ml), ethyl acetate fraction 71.58% (IC50, 16.34 μg/ml), and chloroform fraction 63.65% (IC50, 18.83 μg/ml). Conclusion. This study has revealed that H. abyssinica leaves possess noticeable in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities. |
| doi_str_mv | 10.1155/2021/6652777 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8079187</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2520674623</sourcerecordid><originalsourceid>FETCH-LOGICAL-c448t-746bfd5cce0486ead825314230b8d0e33d0fc7875f2a6ab9be7cb06506beae943</originalsourceid><addsrcrecordid>eNp9kc1uEzEUhUcIRKvSHWtkiQ0SDLXHHtuzQYqi_kSKxIKfreXx3GlcTexie9LmPXgRXoRnwpOECFjgje17Ph3do1MULwl-T0hdX1S4Ihec15UQ4klxWlHCSk4YeXp8U3pSnMd4h_ORhOOGPy9OKG2kYIydFt8XDn21KXj080c5W28HHQFp103f62E0PtpuGi3cyrY2-bDdqTOXrH_MkktoZpLd2GQhIt-jtAI0D2MH6PIxBW3Sjv_khw1k9mqaWO926I2-BWc10u02Ruus0WgJegPxRfGs10OE88N9Vny5uvw8vymXH68X89myNIzJVArG276rjQHMJAfdyarOoSuKW9lhoLTDvRFS1H2luW6bFoRpMa8xb0FDw-hZ8WHvez-2a-hM3jDoQd0Hu9Zhq7y26m_F2ZW69RslsWiIFNngzcEg-G8jxKTWNhoYBu3Aj1FVdSWJxIQ2GX39D3rnx-ByvInCPIepaKbe7SkTfIwB-uMyBKupcTU1rg6NZ_zVnwGO8O9-M_B2D6ys6_SD_b_dL8Oetq0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2520674623</pqid></control><display><type>article</type><title>In Vitro α-Amylase and α-Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Hagenia abyssinica Leaves</title><source>MEDLINE</source><source>Wiley Online Library Open Access</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>PubMed Central Open Access</source><creator>Kifle, Zemene Demelash ; Debeb, Simachew Gidey ; Belayneh, Yaschilal Muche</creator><contributor>Al Attar, Atef M.</contributor><creatorcontrib>Kifle, Zemene Demelash ; Debeb, Simachew Gidey ; Belayneh, Yaschilal Muche ; Al Attar, Atef M.</creatorcontrib><description>Background. The leaves of Hagenia abyssinica have been used in the management of diabetes mellitus in Ethiopian folk medicine. Thus, this study is aimed at investigating the in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the crude extract and solvent fractions of H. abyssinica leaves. Methods. The in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the plant extract were assessed using 3,5-dinitrosalicylic acid (DNSA), p-nitro-phenyl-a-D glucopyranoside (p-NPG), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays, respectively. Each value of percent inhibition of α-amylase, α-glucosidase, and DPPH scavenging effect was presented as means±SEM (n=3). Results. The α-amylase inhibitory activity of the crude extract and solvent fractions was found to be concentration-dependent. The strongest activity was exhibited by the crude extract at the highest concentration with a percentage inhibition of 74.52% (IC50, 14.52 μg/ml) followed by water fraction 68.24% (IC50, 16.31 μg/ml), ethyl acetate fraction 61.57% (IC50, 18.73 μg/ml), and chloroform fraction 56.87% (IC50, 21.57 μg/ml) of H. abyssinica leaves. In the α-glucosidase inhibition assay, the maximum activity was exhibited by the aqueous fraction 62.54% (IC50, 11.67 μg/ml) followed by ethyl acetate fraction 54.97% (IC50, 15.89 μg/ml), crude extract 46.79% (IC50, >16.5 μg/ml), and chloroform fraction 36.44% (IC50, >16.5 μg/ml). In the antioxidant assay, the crude extract exhibited the highest antioxidant activity 86.36% (IC50, 10.25 μg/ml) followed by water fraction 78.59% (IC50, 13.86 μg/ml), ethyl acetate fraction 71.58% (IC50, 16.34 μg/ml), and chloroform fraction 63.65% (IC50, 18.83 μg/ml). Conclusion. This study has revealed that H. abyssinica leaves possess noticeable in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2021/6652777</identifier><identifier>PMID: 33987444</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Acetic acid ; alpha-Amylases - antagonists & inhibitors ; alpha-Amylases - metabolism ; alpha-Glucosidases - metabolism ; Amylases ; Antioxidants ; Antioxidants - pharmacology ; Assaying ; Chloroform ; Diabetes ; Diabetes mellitus ; Drug dosages ; Enzymes ; Ethyl acetate ; Fractions ; Free radicals ; Gene expression ; Glucosidase ; Glycoside Hydrolase Inhibitors - pharmacology ; Hagenia abyssinica ; Herbal medicine ; Hyperglycemia ; In vitro methods and tests ; Insulin resistance ; Kinases ; Leaves ; Phytochemicals - chemistry ; Phytochemicals - pharmacology ; Plant extracts ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Potassium ; Regression analysis ; Rosaceae - chemistry ; Scavenging ; Solvents ; Solvents - chemistry ; α-Amylase ; α-Glucosidase</subject><ispartof>BioMed research international, 2021, Vol.2021 (1), p.6652777-6652777</ispartof><rights>Copyright © 2021 Zemene Demelash Kifle et al.</rights><rights>Copyright © 2021 Zemene Demelash Kifle et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Zemene Demelash Kifle et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-746bfd5cce0486ead825314230b8d0e33d0fc7875f2a6ab9be7cb06506beae943</citedby><cites>FETCH-LOGICAL-c448t-746bfd5cce0486ead825314230b8d0e33d0fc7875f2a6ab9be7cb06506beae943</cites><orcidid>0000-0001-5564-7176 ; 0000-0001-7030-2782</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079187/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079187/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33987444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Al Attar, Atef M.</contributor><creatorcontrib>Kifle, Zemene Demelash</creatorcontrib><creatorcontrib>Debeb, Simachew Gidey</creatorcontrib><creatorcontrib>Belayneh, Yaschilal Muche</creatorcontrib><title>In Vitro α-Amylase and α-Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Hagenia abyssinica Leaves</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Background. The leaves of Hagenia abyssinica have been used in the management of diabetes mellitus in Ethiopian folk medicine. Thus, this study is aimed at investigating the in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the crude extract and solvent fractions of H. abyssinica leaves. Methods. The in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the plant extract were assessed using 3,5-dinitrosalicylic acid (DNSA), p-nitro-phenyl-a-D glucopyranoside (p-NPG), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays, respectively. Each value of percent inhibition of α-amylase, α-glucosidase, and DPPH scavenging effect was presented as means±SEM (n=3). Results. The α-amylase inhibitory activity of the crude extract and solvent fractions was found to be concentration-dependent. The strongest activity was exhibited by the crude extract at the highest concentration with a percentage inhibition of 74.52% (IC50, 14.52 μg/ml) followed by water fraction 68.24% (IC50, 16.31 μg/ml), ethyl acetate fraction 61.57% (IC50, 18.73 μg/ml), and chloroform fraction 56.87% (IC50, 21.57 μg/ml) of H. abyssinica leaves. In the α-glucosidase inhibition assay, the maximum activity was exhibited by the aqueous fraction 62.54% (IC50, 11.67 μg/ml) followed by ethyl acetate fraction 54.97% (IC50, 15.89 μg/ml), crude extract 46.79% (IC50, >16.5 μg/ml), and chloroform fraction 36.44% (IC50, >16.5 μg/ml). In the antioxidant assay, the crude extract exhibited the highest antioxidant activity 86.36% (IC50, 10.25 μg/ml) followed by water fraction 78.59% (IC50, 13.86 μg/ml), ethyl acetate fraction 71.58% (IC50, 16.34 μg/ml), and chloroform fraction 63.65% (IC50, 18.83 μg/ml). Conclusion. This study has revealed that H. abyssinica leaves possess noticeable in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities.</description><subject>Acetic acid</subject><subject>alpha-Amylases - antagonists & inhibitors</subject><subject>alpha-Amylases - metabolism</subject><subject>alpha-Glucosidases - metabolism</subject><subject>Amylases</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Assaying</subject><subject>Chloroform</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Drug dosages</subject><subject>Enzymes</subject><subject>Ethyl acetate</subject><subject>Fractions</subject><subject>Free radicals</subject><subject>Gene expression</subject><subject>Glucosidase</subject><subject>Glycoside Hydrolase Inhibitors - pharmacology</subject><subject>Hagenia abyssinica</subject><subject>Herbal medicine</subject><subject>Hyperglycemia</subject><subject>In vitro methods and tests</subject><subject>Insulin resistance</subject><subject>Kinases</subject><subject>Leaves</subject><subject>Phytochemicals - chemistry</subject><subject>Phytochemicals - pharmacology</subject><subject>Plant extracts</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Potassium</subject><subject>Regression analysis</subject><subject>Rosaceae - chemistry</subject><subject>Scavenging</subject><subject>Solvents</subject><subject>Solvents - chemistry</subject><subject>α-Amylase</subject><subject>α-Glucosidase</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1uEzEUhUcIRKvSHWtkiQ0SDLXHHtuzQYqi_kSKxIKfreXx3GlcTexie9LmPXgRXoRnwpOECFjgje17Ph3do1MULwl-T0hdX1S4Ihec15UQ4klxWlHCSk4YeXp8U3pSnMd4h_ORhOOGPy9OKG2kYIydFt8XDn21KXj080c5W28HHQFp103f62E0PtpuGi3cyrY2-bDdqTOXrH_MkktoZpLd2GQhIt-jtAI0D2MH6PIxBW3Sjv_khw1k9mqaWO926I2-BWc10u02Ruus0WgJegPxRfGs10OE88N9Vny5uvw8vymXH68X89myNIzJVArG276rjQHMJAfdyarOoSuKW9lhoLTDvRFS1H2luW6bFoRpMa8xb0FDw-hZ8WHvez-2a-hM3jDoQd0Hu9Zhq7y26m_F2ZW69RslsWiIFNngzcEg-G8jxKTWNhoYBu3Aj1FVdSWJxIQ2GX39D3rnx-ByvInCPIepaKbe7SkTfIwB-uMyBKupcTU1rg6NZ_zVnwGO8O9-M_B2D6ys6_SD_b_dL8Oetq0</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Kifle, Zemene Demelash</creator><creator>Debeb, Simachew Gidey</creator><creator>Belayneh, Yaschilal Muche</creator><general>Hindawi</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5564-7176</orcidid><orcidid>https://orcid.org/0000-0001-7030-2782</orcidid></search><sort><creationdate>2021</creationdate><title>In Vitro α-Amylase and α-Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Hagenia abyssinica Leaves</title><author>Kifle, Zemene Demelash ; Debeb, Simachew Gidey ; Belayneh, Yaschilal Muche</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-746bfd5cce0486ead825314230b8d0e33d0fc7875f2a6ab9be7cb06506beae943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acetic acid</topic><topic>alpha-Amylases - antagonists & inhibitors</topic><topic>alpha-Amylases - metabolism</topic><topic>alpha-Glucosidases - metabolism</topic><topic>Amylases</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Assaying</topic><topic>Chloroform</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Drug dosages</topic><topic>Enzymes</topic><topic>Ethyl acetate</topic><topic>Fractions</topic><topic>Free radicals</topic><topic>Gene expression</topic><topic>Glucosidase</topic><topic>Glycoside Hydrolase Inhibitors - pharmacology</topic><topic>Hagenia abyssinica</topic><topic>Herbal medicine</topic><topic>Hyperglycemia</topic><topic>In vitro methods and tests</topic><topic>Insulin resistance</topic><topic>Kinases</topic><topic>Leaves</topic><topic>Phytochemicals - chemistry</topic><topic>Phytochemicals - pharmacology</topic><topic>Plant extracts</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Potassium</topic><topic>Regression analysis</topic><topic>Rosaceae - chemistry</topic><topic>Scavenging</topic><topic>Solvents</topic><topic>Solvents - chemistry</topic><topic>α-Amylase</topic><topic>α-Glucosidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kifle, Zemene Demelash</creatorcontrib><creatorcontrib>Debeb, Simachew Gidey</creatorcontrib><creatorcontrib>Belayneh, Yaschilal Muche</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kifle, Zemene Demelash</au><au>Debeb, Simachew Gidey</au><au>Belayneh, Yaschilal Muche</au><au>Al Attar, Atef M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vitro α-Amylase and α-Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Hagenia abyssinica Leaves</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2021</date><risdate>2021</risdate><volume>2021</volume><issue>1</issue><spage>6652777</spage><epage>6652777</epage><pages>6652777-6652777</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Background. The leaves of Hagenia abyssinica have been used in the management of diabetes mellitus in Ethiopian folk medicine. Thus, this study is aimed at investigating the in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the crude extract and solvent fractions of H. abyssinica leaves. Methods. The in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities of the plant extract were assessed using 3,5-dinitrosalicylic acid (DNSA), p-nitro-phenyl-a-D glucopyranoside (p-NPG), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays, respectively. Each value of percent inhibition of α-amylase, α-glucosidase, and DPPH scavenging effect was presented as means±SEM (n=3). Results. The α-amylase inhibitory activity of the crude extract and solvent fractions was found to be concentration-dependent. The strongest activity was exhibited by the crude extract at the highest concentration with a percentage inhibition of 74.52% (IC50, 14.52 μg/ml) followed by water fraction 68.24% (IC50, 16.31 μg/ml), ethyl acetate fraction 61.57% (IC50, 18.73 μg/ml), and chloroform fraction 56.87% (IC50, 21.57 μg/ml) of H. abyssinica leaves. In the α-glucosidase inhibition assay, the maximum activity was exhibited by the aqueous fraction 62.54% (IC50, 11.67 μg/ml) followed by ethyl acetate fraction 54.97% (IC50, 15.89 μg/ml), crude extract 46.79% (IC50, >16.5 μg/ml), and chloroform fraction 36.44% (IC50, >16.5 μg/ml). In the antioxidant assay, the crude extract exhibited the highest antioxidant activity 86.36% (IC50, 10.25 μg/ml) followed by water fraction 78.59% (IC50, 13.86 μg/ml), ethyl acetate fraction 71.58% (IC50, 16.34 μg/ml), and chloroform fraction 63.65% (IC50, 18.83 μg/ml). Conclusion. This study has revealed that H. abyssinica leaves possess noticeable in vitro α-amylase and α-glucosidase inhibitory and antioxidant activities.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>33987444</pmid><doi>10.1155/2021/6652777</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5564-7176</orcidid><orcidid>https://orcid.org/0000-0001-7030-2782</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2314-6133 |
| ispartof | BioMed research international, 2021, Vol.2021 (1), p.6652777-6652777 |
| issn | 2314-6133 2314-6141 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8079187 |
| source | MEDLINE; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Acetic acid alpha-Amylases - antagonists & inhibitors alpha-Amylases - metabolism alpha-Glucosidases - metabolism Amylases Antioxidants Antioxidants - pharmacology Assaying Chloroform Diabetes Diabetes mellitus Drug dosages Enzymes Ethyl acetate Fractions Free radicals Gene expression Glucosidase Glycoside Hydrolase Inhibitors - pharmacology Hagenia abyssinica Herbal medicine Hyperglycemia In vitro methods and tests Insulin resistance Kinases Leaves Phytochemicals - chemistry Phytochemicals - pharmacology Plant extracts Plant Extracts - chemistry Plant Extracts - pharmacology Plant Leaves - chemistry Potassium Regression analysis Rosaceae - chemistry Scavenging Solvents Solvents - chemistry α-Amylase α-Glucosidase |
| title | In Vitro α-Amylase and α-Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Hagenia abyssinica Leaves |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T23%3A24%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20Vitro%20%CE%B1-Amylase%20and%20%CE%B1-Glucosidase%20Inhibitory%20and%20Antioxidant%20Activities%20of%20the%20Crude%20Extract%20and%20Solvent%20Fractions%20of%20Hagenia%20abyssinica%20Leaves&rft.jtitle=BioMed%20research%20international&rft.au=Kifle,%20Zemene%20Demelash&rft.date=2021&rft.volume=2021&rft.issue=1&rft.spage=6652777&rft.epage=6652777&rft.pages=6652777-6652777&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2021/6652777&rft_dat=%3Cproquest_pubme%3E2520674623%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2520674623&rft_id=info:pmid/33987444&rfr_iscdi=true |