Protein‐induced conformational change in glycans decreases the resolution of glycoproteins in hydrophilic interaction liquid chromatography

An understanding of why hydrophilic interaction liquid chromatography gives a higher resolution for glycans than for glycoproteins would facilitate column improvements. Separations of the glycoforms of ribonuclease B compared to its released glycans were studied using a commercial hydrophilic intera...

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Veröffentlicht in:	Journal of separation science 2021-04, Vol.44 (8), p.1581-1591
Hauptverfasser:	Bupp, Charles R., Schwartz, Cameron, Wei, Bingchuan, Wirth, Mary J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbohydrate Conformation Chromatography Chromatography, Liquid Column chromatography Copolymers Glycan glycoprofiling Glycoproteins Glycoproteins - chemistry Glycoproteins - isolation & purification Glycoproteins - metabolism Hydrogen bonding hydrophilic interaction liquid chromatography Hydrophilicity Hydrophobic and Hydrophilic Interactions intact glycoprotein Liquid chromatography Mannose Polysaccharides - analysis Polysaccharides - metabolism Proteins ribonuclease B Ribonucleases - chemistry Ribonucleases - isolation & purification Ribonucleases - metabolism Selectivity Topology Water chemistry
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