Increasing Natural Resistance Associated Macrophage Protein 1 serum level after Miana treatment in BALB/c induced Klebsiella pneumoniae experimental research
Miana (Coleus Scutellariodes [L] Benth) inhibits growth of bacterial pathogen inside macrophage. The aim of this study is to determine the protein level of Natural Resistance Associated Macrophage Protein 1 (NRAMP1), after administration of Miana extracts in BALB/c mice induced Klebsiella pneumoniae...
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| Veröffentlicht in: | Annals of medicine and surgery 2021-05, Vol.65, p.102262-102262, Article 102262 |
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| container_title | Annals of medicine and surgery |
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| creator | Wahyuni, Titis Dewi Hatta, Mochammad Bukhari, Agussalim Santoso, Arif Massi, Muhammad Nasrum |
| description | Miana (Coleus Scutellariodes [L] Benth) inhibits growth of bacterial pathogen inside macrophage.
The aim of this study is to determine the protein level of Natural Resistance Associated Macrophage Protein 1 (NRAMP1), after administration of Miana extracts in BALB/c mice induced Klebsiella pneumoniae.
This is an experimental study using animal model with post test-only controlled group design. Twenty healthy adult male BALB/c mice were randomly divided into four groups, negative control group (distilled water), Levofloxacin 100 mg/kg, injection intraperitoneal, first treatment group (Miana leaves extract/MLE 510 mg/kg) and second treatment group (Miana + levofloxacin). MLE were administered via gastric gavage for ten consecutive days. The blood was drawn from each mice on the first day, on the eight day of experiment (2 h after treatment), and at 10 days. The blood sample was examined by ELISA to determine the NRAMP1 protein level. Analysis of the number of lung tissue bacteria used Plate count agar to see the growth of Klebsiella pneumonia.
NRAMP1 protein level in BALB/c mice after administration of Miana extract was increased significantly in after 10 days treatment (p |
| doi_str_mv | 10.1016/j.amsu.2021.102262 |
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The aim of this study is to determine the protein level of Natural Resistance Associated Macrophage Protein 1 (NRAMP1), after administration of Miana extracts in BALB/c mice induced Klebsiella pneumoniae.
This is an experimental study using animal model with post test-only controlled group design. Twenty healthy adult male BALB/c mice were randomly divided into four groups, negative control group (distilled water), Levofloxacin 100 mg/kg, injection intraperitoneal, first treatment group (Miana leaves extract/MLE 510 mg/kg) and second treatment group (Miana + levofloxacin). MLE were administered via gastric gavage for ten consecutive days. The blood was drawn from each mice on the first day, on the eight day of experiment (2 h after treatment), and at 10 days. The blood sample was examined by ELISA to determine the NRAMP1 protein level. Analysis of the number of lung tissue bacteria used Plate count agar to see the growth of Klebsiella pneumonia.
NRAMP1 protein level in BALB/c mice after administration of Miana extract was increased significantly in after 10 days treatment (p < 0.0001). The highest increasing in protein levels was found in treatment group (Miana + levofloxacin) with an increase before treatment 3036,07 to 10010,30 pg/ml after treatment p < 0,0001.
NRAMP1 protein level in BALB/c mice were highest increasing in protein levels after administration of Miana extract and Levofloxacin compared Miana or Levofloxacin only and clinical impact proved a comparable effect on suppressing Klebsiella pneumoniae growth.
The institutional protocol number of this study is 1010/UN4.6.4.5.31/PP36/2019.
•NRAMP1 was increased significantly in after 10 days treatment of Miana (Coleus scutellaroides (L) Benth).•NRAMP1 protein level in were highest increasing after administration of Miana and Levofloxacin compared Miana or Levofloxacin only.•The administration of Levofloxacin and Miana proved a comparable effect on suppressing Klebsiella pneumoniae growth.•Further study needed to explore in the mRNA expression of NRAMP1 gene after administration of Levofloxacin and Miana.</description><identifier>ISSN: 2049-0801</identifier><identifier>EISSN: 2049-0801</identifier><identifier>DOI: 10.1016/j.amsu.2021.102262</identifier><identifier>PMID: 33898034</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>BALB/c mice ; Experimental Research ; Levofloxacin ; Miana leaves extract ; NRAMP1 protein</subject><ispartof>Annals of medicine and surgery, 2021-05, Vol.65, p.102262-102262, Article 102262</ispartof><rights>2021 The Authors</rights><rights>2021 The Authors.</rights><rights>2021 The Authors 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-e26e77c79633e4fb7020d9ca6c34c2ff66e576c8edf98c15bcdb678af704e7c03</citedby><orcidid>0000-0002-8456-4203 ; 0000-0002-6340-8615</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058902/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058902/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33898034$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wahyuni, Titis Dewi</creatorcontrib><creatorcontrib>Hatta, Mochammad</creatorcontrib><creatorcontrib>Bukhari, Agussalim</creatorcontrib><creatorcontrib>Santoso, Arif</creatorcontrib><creatorcontrib>Massi, Muhammad Nasrum</creatorcontrib><title>Increasing Natural Resistance Associated Macrophage Protein 1 serum level after Miana treatment in BALB/c induced Klebsiella pneumoniae experimental research</title><title>Annals of medicine and surgery</title><addtitle>Ann Med Surg (Lond)</addtitle><description>Miana (Coleus Scutellariodes [L] Benth) inhibits growth of bacterial pathogen inside macrophage.
The aim of this study is to determine the protein level of Natural Resistance Associated Macrophage Protein 1 (NRAMP1), after administration of Miana extracts in BALB/c mice induced Klebsiella pneumoniae.
This is an experimental study using animal model with post test-only controlled group design. Twenty healthy adult male BALB/c mice were randomly divided into four groups, negative control group (distilled water), Levofloxacin 100 mg/kg, injection intraperitoneal, first treatment group (Miana leaves extract/MLE 510 mg/kg) and second treatment group (Miana + levofloxacin). MLE were administered via gastric gavage for ten consecutive days. The blood was drawn from each mice on the first day, on the eight day of experiment (2 h after treatment), and at 10 days. The blood sample was examined by ELISA to determine the NRAMP1 protein level. Analysis of the number of lung tissue bacteria used Plate count agar to see the growth of Klebsiella pneumonia.
NRAMP1 protein level in BALB/c mice after administration of Miana extract was increased significantly in after 10 days treatment (p < 0.0001). The highest increasing in protein levels was found in treatment group (Miana + levofloxacin) with an increase before treatment 3036,07 to 10010,30 pg/ml after treatment p < 0,0001.
NRAMP1 protein level in BALB/c mice were highest increasing in protein levels after administration of Miana extract and Levofloxacin compared Miana or Levofloxacin only and clinical impact proved a comparable effect on suppressing Klebsiella pneumoniae growth.
The institutional protocol number of this study is 1010/UN4.6.4.5.31/PP36/2019.
•NRAMP1 was increased significantly in after 10 days treatment of Miana (Coleus scutellaroides (L) Benth).•NRAMP1 protein level in were highest increasing after administration of Miana and Levofloxacin compared Miana or Levofloxacin only.•The administration of Levofloxacin and Miana proved a comparable effect on suppressing Klebsiella pneumoniae growth.•Further study needed to explore in the mRNA expression of NRAMP1 gene after administration of Levofloxacin and Miana.</description><subject>BALB/c mice</subject><subject>Experimental Research</subject><subject>Levofloxacin</subject><subject>Miana leaves extract</subject><subject>NRAMP1 protein</subject><issn>2049-0801</issn><issn>2049-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9UcFu1DAUjBCIVqU_wAH5yGW3tpM4joSQtlWhFVtACM7W25eXXa8SJ9jOCj6Gf8XRtlW5cPKTPTNvPJNlrwVfCi7UxX4JfZiWkkuRLqRU8ll2KnlRL7jm4vmT-SQ7D2HPORe8zJXSL7OTPNe15nlxmv25degJgnVb9hni5KFj3yjYEMEhsVUIA1qI1LA7QD-MO9gS--qHSNYxwQL5qWcdHahj0Eby7M6CAxaTZuzJRZZgl6v15QWmqZkwCX3qaBMsdR2w0dHUD84CMfo1krczJTnwFAg87l5lL1roAp3fn2fZjw_X369uFusvH2-vVusFFmUZFyQVVRVWtcpzKtpNxSVvagSFeYGybZWislKoqWlrjaLcYLNRlYa24gVVyPOz7P1Rd5w2PTWYXKQgzJgMgf9tBrDm3xdnd2Y7HIzmpa65TAJv7wX88HOiEE1vA85_dDRMwchS6CqFr4oElUdoijMET-3jGsHNXK3Zm7laM1drjtUm0punBh8pD0UmwLsjgFJMB0veBLSUKmysJ4ymGez_9P8CY6a5fw</recordid><startdate>20210501</startdate><enddate>20210501</enddate><creator>Wahyuni, Titis Dewi</creator><creator>Hatta, Mochammad</creator><creator>Bukhari, Agussalim</creator><creator>Santoso, Arif</creator><creator>Massi, Muhammad Nasrum</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8456-4203</orcidid><orcidid>https://orcid.org/0000-0002-6340-8615</orcidid></search><sort><creationdate>20210501</creationdate><title>Increasing Natural Resistance Associated Macrophage Protein 1 serum level after Miana treatment in BALB/c induced Klebsiella pneumoniae experimental research</title><author>Wahyuni, Titis Dewi ; Hatta, Mochammad ; Bukhari, Agussalim ; Santoso, Arif ; Massi, Muhammad Nasrum</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-e26e77c79633e4fb7020d9ca6c34c2ff66e576c8edf98c15bcdb678af704e7c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>BALB/c mice</topic><topic>Experimental Research</topic><topic>Levofloxacin</topic><topic>Miana leaves extract</topic><topic>NRAMP1 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wahyuni, Titis Dewi</creatorcontrib><creatorcontrib>Hatta, Mochammad</creatorcontrib><creatorcontrib>Bukhari, Agussalim</creatorcontrib><creatorcontrib>Santoso, Arif</creatorcontrib><creatorcontrib>Massi, Muhammad Nasrum</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Annals of medicine and surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wahyuni, Titis Dewi</au><au>Hatta, Mochammad</au><au>Bukhari, Agussalim</au><au>Santoso, Arif</au><au>Massi, Muhammad Nasrum</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increasing Natural Resistance Associated Macrophage Protein 1 serum level after Miana treatment in BALB/c induced Klebsiella pneumoniae experimental research</atitle><jtitle>Annals of medicine and surgery</jtitle><addtitle>Ann Med Surg (Lond)</addtitle><date>2021-05-01</date><risdate>2021</risdate><volume>65</volume><spage>102262</spage><epage>102262</epage><pages>102262-102262</pages><artnum>102262</artnum><issn>2049-0801</issn><eissn>2049-0801</eissn><abstract>Miana (Coleus Scutellariodes [L] Benth) inhibits growth of bacterial pathogen inside macrophage.
The aim of this study is to determine the protein level of Natural Resistance Associated Macrophage Protein 1 (NRAMP1), after administration of Miana extracts in BALB/c mice induced Klebsiella pneumoniae.
This is an experimental study using animal model with post test-only controlled group design. Twenty healthy adult male BALB/c mice were randomly divided into four groups, negative control group (distilled water), Levofloxacin 100 mg/kg, injection intraperitoneal, first treatment group (Miana leaves extract/MLE 510 mg/kg) and second treatment group (Miana + levofloxacin). MLE were administered via gastric gavage for ten consecutive days. The blood was drawn from each mice on the first day, on the eight day of experiment (2 h after treatment), and at 10 days. The blood sample was examined by ELISA to determine the NRAMP1 protein level. Analysis of the number of lung tissue bacteria used Plate count agar to see the growth of Klebsiella pneumonia.
NRAMP1 protein level in BALB/c mice after administration of Miana extract was increased significantly in after 10 days treatment (p < 0.0001). The highest increasing in protein levels was found in treatment group (Miana + levofloxacin) with an increase before treatment 3036,07 to 10010,30 pg/ml after treatment p < 0,0001.
NRAMP1 protein level in BALB/c mice were highest increasing in protein levels after administration of Miana extract and Levofloxacin compared Miana or Levofloxacin only and clinical impact proved a comparable effect on suppressing Klebsiella pneumoniae growth.
The institutional protocol number of this study is 1010/UN4.6.4.5.31/PP36/2019.
•NRAMP1 was increased significantly in after 10 days treatment of Miana (Coleus scutellaroides (L) Benth).•NRAMP1 protein level in were highest increasing after administration of Miana and Levofloxacin compared Miana or Levofloxacin only.•The administration of Levofloxacin and Miana proved a comparable effect on suppressing Klebsiella pneumoniae growth.•Further study needed to explore in the mRNA expression of NRAMP1 gene after administration of Levofloxacin and Miana.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>33898034</pmid><doi>10.1016/j.amsu.2021.102262</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-8456-4203</orcidid><orcidid>https://orcid.org/0000-0002-6340-8615</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | BALB/c mice Experimental Research Levofloxacin Miana leaves extract NRAMP1 protein |
| title | Increasing Natural Resistance Associated Macrophage Protein 1 serum level after Miana treatment in BALB/c induced Klebsiella pneumoniae experimental research |
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