Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts

Key message Modified pEAQ-HT-DEST1 vectors were used for agroinfiltration in legumes. We demonstrate protein expression and export in pea, lentil, and faba bean; however, the method for chickpea was not successful. Agroinfiltration is a valuable research method for investigating virulence and avirul...

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Veröffentlicht in:	Plant cell reports 2021-05, Vol.40 (5), p.805-818
Hauptverfasser:	Debler, Johannes W., Henares, Bernadette M., Lee, Robert C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens - genetics Apoplast Beans Biomedical and Life Sciences Biotechnology Broad beans Cell Biology Chickpeas Confocal microscopy Effectors Expression vectors Fabaceae - genetics Fabaceae - metabolism Fluorescence Gene expression Gene Expression Regulation, Plant Genetic transformation Green fluorescent protein Histidine Leaves Legumes Life Sciences Microscopy, Confocal Necrosis Nicotiana - genetics Nicotiana - metabolism Original Original Article Pathogens Peas Peptides Pests Plant Biochemistry Plant Sciences Plant species Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Protein transport Proteins Vicia faba - genetics Vicia faba - metabolism Virulence
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