In situ hybridization for the detection of infectious laryngotracheitis virus in sections of trachea from experimentally infected chickens
An in situ hybridization procedure for the detection of infectious laryngotracheitis virus (ILTV) in experimentally infected chickens is described. Formalin-fixed, paraffin-embedded sections of trachea, taken from chickens on days 3-10 post-inoculation (p.i.) with ILTV were hybridized with a mixture...
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| Veröffentlicht in: | Acta veterinaria scandinavica 1998-01, Vol.39 (4), p.415-421 |
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| creator | Nielsen, O L Handberg, K J Jørgensen, P H |
| description | An in situ hybridization procedure for the detection of infectious laryngotracheitis virus (ILTV) in experimentally infected chickens is described. Formalin-fixed, paraffin-embedded sections of trachea, taken from chickens on days 3-10 post-inoculation (p.i.) with ILTV were hybridized with a mixture of 2 biotinylated, polymerase chain reaction-generated DNA fragments. The fragments correspond to sequences of the ILTV glycoprotein C and thymidine Kinase genes. In situ hybridization was seen in 7 out of 7 chickens examined on day 3 p.i., 2 out of 2 examined on day 4 p.i. and 3 out of 3 examined on day 5 p.i. No hybridization was observed in 3 out of 3 chickens examined on day 10 p.i. ILTV nucleic acid was detected in nuclei of degenerated tracheal epithelial cells and in intranuclear inclusion bodies of syncytia. |
| doi_str_mv | 10.1186/BF03547767 |
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Formalin-fixed, paraffin-embedded sections of trachea, taken from chickens on days 3-10 post-inoculation (p.i.) with ILTV were hybridized with a mixture of 2 biotinylated, polymerase chain reaction-generated DNA fragments. The fragments correspond to sequences of the ILTV glycoprotein C and thymidine Kinase genes. In situ hybridization was seen in 7 out of 7 chickens examined on day 3 p.i., 2 out of 2 examined on day 4 p.i. and 3 out of 3 examined on day 5 p.i. No hybridization was observed in 3 out of 3 chickens examined on day 10 p.i. ILTV nucleic acid was detected in nuclei of degenerated tracheal epithelial cells and in intranuclear inclusion bodies of syncytia.</description><identifier>ISSN: 0044-605X</identifier><identifier>ISSN: 1751-0147</identifier><identifier>EISSN: 1751-0147</identifier><identifier>DOI: 10.1186/BF03547767</identifier><identifier>PMID: 9926455</identifier><language>eng</language><publisher>England: BioMed Central</publisher><subject>Administration, Oral ; Animals ; Antibodies, Viral - blood ; Chickens ; DNA Primers - chemistry ; DNA, Viral - analysis ; DNA, Viral - chemistry ; Enzyme-Linked Immunosorbent Assay - veterinary ; Herpesviridae Infections - diagnosis ; Herpesviridae Infections - veterinary ; Herpesviridae Infections - virology ; Herpesvirus 1, Gallid - genetics ; Herpesvirus 1, Gallid - immunology ; Herpesvirus 1, Gallid - isolation & purification ; In Situ Hybridization - veterinary ; Polymerase Chain Reaction - veterinary ; Poultry Diseases - virology ; Specific Pathogen-Free Organisms - immunology ; Trachea - pathology ; Trachea - virology ; Tracheal Diseases - diagnosis ; Tracheal Diseases - veterinary ; Tracheal Diseases - virology</subject><ispartof>Acta veterinaria scandinavica, 1998-01, Vol.39 (4), p.415-421</ispartof><rights>The Author(s) 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-163dfd8096c33d798263b12dbbb1970f957476a1c53328a9877f972ca6bb24c63</citedby><cites>FETCH-LOGICAL-c373t-163dfd8096c33d798263b12dbbb1970f957476a1c53328a9877f972ca6bb24c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050672/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050672/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9926455$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nielsen, O L</creatorcontrib><creatorcontrib>Handberg, K J</creatorcontrib><creatorcontrib>Jørgensen, P H</creatorcontrib><title>In situ hybridization for the detection of infectious laryngotracheitis virus in sections of trachea from experimentally infected chickens</title><title>Acta veterinaria scandinavica</title><addtitle>Acta Vet Scand</addtitle><description>An in situ hybridization procedure for the detection of infectious laryngotracheitis virus (ILTV) in experimentally infected chickens is described. Formalin-fixed, paraffin-embedded sections of trachea, taken from chickens on days 3-10 post-inoculation (p.i.) with ILTV were hybridized with a mixture of 2 biotinylated, polymerase chain reaction-generated DNA fragments. The fragments correspond to sequences of the ILTV glycoprotein C and thymidine Kinase genes. In situ hybridization was seen in 7 out of 7 chickens examined on day 3 p.i., 2 out of 2 examined on day 4 p.i. and 3 out of 3 examined on day 5 p.i. No hybridization was observed in 3 out of 3 chickens examined on day 10 p.i. ILTV nucleic acid was detected in nuclei of degenerated tracheal epithelial cells and in intranuclear inclusion bodies of syncytia.</description><subject>Administration, Oral</subject><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Chickens</subject><subject>DNA Primers - chemistry</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - chemistry</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Herpesviridae Infections - diagnosis</subject><subject>Herpesviridae Infections - veterinary</subject><subject>Herpesviridae Infections - virology</subject><subject>Herpesvirus 1, Gallid - genetics</subject><subject>Herpesvirus 1, Gallid - immunology</subject><subject>Herpesvirus 1, Gallid - isolation & purification</subject><subject>In Situ Hybridization - veterinary</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Poultry Diseases - virology</subject><subject>Specific Pathogen-Free Organisms - immunology</subject><subject>Trachea - pathology</subject><subject>Trachea - virology</subject><subject>Tracheal Diseases - diagnosis</subject><subject>Tracheal Diseases - veterinary</subject><subject>Tracheal Diseases - virology</subject><issn>0044-605X</issn><issn>1751-0147</issn><issn>1751-0147</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUtPxCAUhYnR6PjYuDdh5cKkCqVA2Zio8ZWYuNHEHaEULNopIzDG8Sf4q2Ue8bEi995zPw4cAPYxOsa4ZifnV4jQinPG18AIc4oLhCu-DkYIVVXBEH3aAtsxvuSSVSXbBJtClKyidAS-bgcYXZrCbtYE17pPlZwfoPUBps7A1iSjFx1voRvsophG2KswG559Ckp3xiUX4bsLue8ybbkQ5xvLuYI2-DE0HxMT3NgMSfX9bEUzLdSd069miLtgw6o-mr3VuQMery4fLm6Ku_vr24uzu0ITTlKBGWltWyPBNCEtF3XJSIPLtmkaLDiygvKKM4U1JaSslag5t4KXWrGmKSvNyA44XXIn02ZsWp0NBdXLSfaWXyW9cvL_ZHCdfPbvskYUMV5mwOEKEPzb1MQkxy5q0_dqMPlzJBOYspxHFh4thTr4GIOxP5dgJOfJyd_ksvjgr60f6Soq8g03HZgI</recordid><startdate>19980101</startdate><enddate>19980101</enddate><creator>Nielsen, O L</creator><creator>Handberg, K J</creator><creator>Jørgensen, P H</creator><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980101</creationdate><title>In situ hybridization for the detection of infectious laryngotracheitis virus in sections of trachea from experimentally infected chickens</title><author>Nielsen, O L ; Handberg, K J ; Jørgensen, P H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-163dfd8096c33d798263b12dbbb1970f957476a1c53328a9877f972ca6bb24c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Administration, Oral</topic><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Chickens</topic><topic>DNA Primers - chemistry</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - chemistry</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Herpesviridae Infections - diagnosis</topic><topic>Herpesviridae Infections - veterinary</topic><topic>Herpesviridae Infections - virology</topic><topic>Herpesvirus 1, Gallid - genetics</topic><topic>Herpesvirus 1, Gallid - immunology</topic><topic>Herpesvirus 1, Gallid - isolation & purification</topic><topic>In Situ Hybridization - veterinary</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Poultry Diseases - virology</topic><topic>Specific Pathogen-Free Organisms - immunology</topic><topic>Trachea - pathology</topic><topic>Trachea - virology</topic><topic>Tracheal Diseases - diagnosis</topic><topic>Tracheal Diseases - veterinary</topic><topic>Tracheal Diseases - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nielsen, O L</creatorcontrib><creatorcontrib>Handberg, K J</creatorcontrib><creatorcontrib>Jørgensen, P H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta veterinaria scandinavica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nielsen, O L</au><au>Handberg, K J</au><au>Jørgensen, P H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In situ hybridization for the detection of infectious laryngotracheitis virus in sections of trachea from experimentally infected chickens</atitle><jtitle>Acta veterinaria scandinavica</jtitle><addtitle>Acta Vet Scand</addtitle><date>1998-01-01</date><risdate>1998</risdate><volume>39</volume><issue>4</issue><spage>415</spage><epage>421</epage><pages>415-421</pages><issn>0044-605X</issn><issn>1751-0147</issn><eissn>1751-0147</eissn><abstract>An in situ hybridization procedure for the detection of infectious laryngotracheitis virus (ILTV) in experimentally infected chickens is described. Formalin-fixed, paraffin-embedded sections of trachea, taken from chickens on days 3-10 post-inoculation (p.i.) with ILTV were hybridized with a mixture of 2 biotinylated, polymerase chain reaction-generated DNA fragments. The fragments correspond to sequences of the ILTV glycoprotein C and thymidine Kinase genes. In situ hybridization was seen in 7 out of 7 chickens examined on day 3 p.i., 2 out of 2 examined on day 4 p.i. and 3 out of 3 examined on day 5 p.i. No hybridization was observed in 3 out of 3 chickens examined on day 10 p.i. ILTV nucleic acid was detected in nuclei of degenerated tracheal epithelial cells and in intranuclear inclusion bodies of syncytia.</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>9926455</pmid><doi>10.1186/BF03547767</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Administration, Oral Animals Antibodies, Viral - blood Chickens DNA Primers - chemistry DNA, Viral - analysis DNA, Viral - chemistry Enzyme-Linked Immunosorbent Assay - veterinary Herpesviridae Infections - diagnosis Herpesviridae Infections - veterinary Herpesviridae Infections - virology Herpesvirus 1, Gallid - genetics Herpesvirus 1, Gallid - immunology Herpesvirus 1, Gallid - isolation & purification In Situ Hybridization - veterinary Polymerase Chain Reaction - veterinary Poultry Diseases - virology Specific Pathogen-Free Organisms - immunology Trachea - pathology Trachea - virology Tracheal Diseases - diagnosis Tracheal Diseases - veterinary Tracheal Diseases - virology |
| title | In situ hybridization for the detection of infectious laryngotracheitis virus in sections of trachea from experimentally infected chickens |
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