Forensic evaluation of two nucleic acid extraction systems and validation of a RT-qPCR protocol for identification of SARS-CoV-2 in post-mortem nasopharyngeal swabs

•The INTCF has validated the molecular detection of SARS-CoV-2 for its forensic use.•Automated virus RNA extraction has greater relative efficiency than the manual one.•Molecular detection of SARS-CoV-2 with the kit tested is accurate and sensitive. The COVID-19 outbreak has represented a challenge...

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Veröffentlicht in:	Forensic science international 2021-06, Vol.323, p.110775-110775, Article 110775
Hauptverfasser:	Barrio, Pedro A., Fernández-Rodríguez, Amparo, Martín, Pablo, Fernández, Coro, Fernández, Lourdes, Alonso, Antonio
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Autopsies Autopsy Contamination Coronavirus Infectious Disease-19 (COVID-19) Coronaviruses COVID-19 Cross-reactivity Extraction procedures Forensic genetics Forensic microbiology Forensic science Forensic sciences Influenza Nucleic acids Polymerase chain reaction Quantitative reverse transcription PCR (RT-qPCR) Reproducibility Research Paper Reverse transcription Ribonucleic acid RNA RNA viruses Robots Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Toxicology Validation Viral diseases Viruses
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