Extended culture and imaging of normal and regenerating adult zebrafish hearts in a fluidic device
Myocardial infarction and heart failure are leading causes of death worldwide, in large part because adult human myocardium has extremely limited regeneration capacity. Zebrafish are a powerful model for identifying new strategies for human cardiac repair because their hearts regenerate after relati...
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| Veröffentlicht in: | Lab on a chip 2020-01, Vol.2 (2), p.274-284 |
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| creator | Yip, Joycelyn K Harrison, Michael Villafuerte, Jessi Fernandez, G. Esteban Petersen, Andrew P Lien, Ching-Ling McCain, Megan L |
| description | Myocardial infarction and heart failure are leading causes of death worldwide, in large part because adult human myocardium has extremely limited regeneration capacity. Zebrafish are a powerful model for identifying new strategies for human cardiac repair because their hearts regenerate after relatively severe injuries. Zebrafish are also relatively scalable and compatible with many genetic tools. However, characterizing the regeneration process in live adult zebrafish hearts has proved challenging because adult fish are opaque, preventing live imaging
in vivo
. An alternative strategy is to explant and culture intact adult zebrafish hearts and investigate them
ex vivo
. However, explanted hearts maintained in conventional culture conditions experience rapid declines in morphology and physiology. To overcome these limitations, we designed and fabricated a fluidic device for culturing explanted adult zebrafish hearts with constant media perfusion that is also compatible with live imaging. We then compared the morphology and calcium activity of hearts cultured in the device, hearts cultured statically in dishes, and freshly explanted hearts. After one week of culture, hearts in the device experienced significantly less morphological degradation compared to hearts cultured in dishes. Hearts cultured in devices for one week also maintained capture rates similar to fresh hearts, unlike hearts cultured in dishes. We then cultured explanted injured hearts in the device and used live imaging techniques to continuously record the myocardial revascularization process over several days, demonstrating how our device is compatible with long-term live imaging and thereby enables unprecedented visual access to the multi-day process of adult zebrafish heart regeneration.
Culturing adult zebrafish hearts in a fluidic device reduces morphological and functional declines and enables live imaging of heart regeneration. |
| doi_str_mv | 10.1039/c9lc01044k |
| format | Article |
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in vivo
. An alternative strategy is to explant and culture intact adult zebrafish hearts and investigate them
ex vivo
. However, explanted hearts maintained in conventional culture conditions experience rapid declines in morphology and physiology. To overcome these limitations, we designed and fabricated a fluidic device for culturing explanted adult zebrafish hearts with constant media perfusion that is also compatible with live imaging. We then compared the morphology and calcium activity of hearts cultured in the device, hearts cultured statically in dishes, and freshly explanted hearts. After one week of culture, hearts in the device experienced significantly less morphological degradation compared to hearts cultured in dishes. Hearts cultured in devices for one week also maintained capture rates similar to fresh hearts, unlike hearts cultured in dishes. We then cultured explanted injured hearts in the device and used live imaging techniques to continuously record the myocardial revascularization process over several days, demonstrating how our device is compatible with long-term live imaging and thereby enables unprecedented visual access to the multi-day process of adult zebrafish heart regeneration.
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in vivo
. An alternative strategy is to explant and culture intact adult zebrafish hearts and investigate them
ex vivo
. However, explanted hearts maintained in conventional culture conditions experience rapid declines in morphology and physiology. To overcome these limitations, we designed and fabricated a fluidic device for culturing explanted adult zebrafish hearts with constant media perfusion that is also compatible with live imaging. We then compared the morphology and calcium activity of hearts cultured in the device, hearts cultured statically in dishes, and freshly explanted hearts. After one week of culture, hearts in the device experienced significantly less morphological degradation compared to hearts cultured in dishes. Hearts cultured in devices for one week also maintained capture rates similar to fresh hearts, unlike hearts cultured in dishes. We then cultured explanted injured hearts in the device and used live imaging techniques to continuously record the myocardial revascularization process over several days, demonstrating how our device is compatible with long-term live imaging and thereby enables unprecedented visual access to the multi-day process of adult zebrafish heart regeneration.
Culturing adult zebrafish hearts in a fluidic device reduces morphological and functional declines and enables live imaging of heart regeneration.</description><subject>Animals</subject><subject>Compatibility</subject><subject>Culture</subject><subject>Heart - diagnostic imaging</subject><subject>Imaging</subject><subject>Imaging techniques</subject><subject>Lab-On-A-Chip Devices</subject><subject>Morphology</subject><subject>Myocardial infarction</subject><subject>Myocardium</subject><subject>Regeneration</subject><subject>Tissue Culture Techniques</subject><subject>Zebrafish</subject><issn>1473-0197</issn><issn>1473-0189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtPGzEURi0EApqyYd_KqBuElNaveXiDhCIeFZG6adeWH3cS04kn2DOI9tfjkBBoF6xs-Rx9utcfQseUfKWEy29WtpZQIsTvHXRIRcXHhNZyd3uX1QH6kNIdIbQQZb2PDjitK8YIOUTm8rGH4MBhO7T9EAHr4LBf6JkPM9w1OHRxodvn1wgzCBB1v0LaZR__BRN149Mcz0HHPmEfsMZNO3jnLXbw4C18RHuNbhMcbc4R-nV1-XNyM57-uP4-uZiOrZC0H9umNlYTJoUuG1FQoEaQwjBimDCOMiiFZJKUFJyzUkoOTHBnKSmZkbLUfITO17nLwSzAWQh91K1axrxN_KM67dW_JPi5mnUPqs7_UuXAETrdBMTufoDUq4VPFtpWB-iGpBjnhPNSFkVWv_yn3nVDDHm9bAnGqpzHsnW2tmzsUorQbIehRK2qUxM5nTxXd5vlz2_H36ovXWXh01qIyW7pa_eZn7zH1dI1_AksHKoJ</recordid><startdate>20200121</startdate><enddate>20200121</enddate><creator>Yip, Joycelyn K</creator><creator>Harrison, Michael</creator><creator>Villafuerte, Jessi</creator><creator>Fernandez, G. Esteban</creator><creator>Petersen, Andrew P</creator><creator>Lien, Ching-Ling</creator><creator>McCain, Megan L</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1908-6783</orcidid></search><sort><creationdate>20200121</creationdate><title>Extended culture and imaging of normal and regenerating adult zebrafish hearts in a fluidic device</title><author>Yip, Joycelyn K ; Harrison, Michael ; Villafuerte, Jessi ; Fernandez, G. 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Esteban</creatorcontrib><creatorcontrib>Petersen, Andrew P</creatorcontrib><creatorcontrib>Lien, Ching-Ling</creatorcontrib><creatorcontrib>McCain, Megan L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Lab on a chip</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yip, Joycelyn K</au><au>Harrison, Michael</au><au>Villafuerte, Jessi</au><au>Fernandez, G. 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However, characterizing the regeneration process in live adult zebrafish hearts has proved challenging because adult fish are opaque, preventing live imaging
in vivo
. An alternative strategy is to explant and culture intact adult zebrafish hearts and investigate them
ex vivo
. However, explanted hearts maintained in conventional culture conditions experience rapid declines in morphology and physiology. To overcome these limitations, we designed and fabricated a fluidic device for culturing explanted adult zebrafish hearts with constant media perfusion that is also compatible with live imaging. We then compared the morphology and calcium activity of hearts cultured in the device, hearts cultured statically in dishes, and freshly explanted hearts. After one week of culture, hearts in the device experienced significantly less morphological degradation compared to hearts cultured in dishes. Hearts cultured in devices for one week also maintained capture rates similar to fresh hearts, unlike hearts cultured in dishes. We then cultured explanted injured hearts in the device and used live imaging techniques to continuously record the myocardial revascularization process over several days, demonstrating how our device is compatible with long-term live imaging and thereby enables unprecedented visual access to the multi-day process of adult zebrafish heart regeneration.
Culturing adult zebrafish hearts in a fluidic device reduces morphological and functional declines and enables live imaging of heart regeneration.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>31872200</pmid><doi>10.1039/c9lc01044k</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-1908-6783</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Compatibility Culture Heart - diagnostic imaging Imaging Imaging techniques Lab-On-A-Chip Devices Morphology Myocardial infarction Myocardium Regeneration Tissue Culture Techniques Zebrafish |
| title | Extended culture and imaging of normal and regenerating adult zebrafish hearts in a fluidic device |
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