StRAP2.3, an ERF‐VII transcription factor, directly activates StInvInh2 to enhance cold-induced sweetening resistance in potato
Potato invertase inhibitor ( StInvInh2 ) positively regulates cold-induced sweetening (CIS) resistance by inhibiting the activity of vacuolar invertase. The distinct expression patterns of StInvInh2 have been thoroughly characterized in different potato genotypes, but the related CIS ability has not...
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| Veröffentlicht in: | Horticulture research 2021-04, Vol.8 (1), p.82, Article 82 |
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| creator | Shi, Weiling Song, Yuhao Liu, Tiantian Ma, Qiuqin Yin, Wang Shen, Yuchen Liu, Tengfei Jiang, Chunyan Zhang, Kai Lv, Dianqiu Song, Botao Wang, Jichun Liu, Xun |
| description | Potato invertase inhibitor (
StInvInh2
) positively regulates cold-induced sweetening (CIS) resistance by inhibiting the activity of vacuolar invertase. The distinct expression patterns of
StInvInh2
have been thoroughly characterized in different potato genotypes, but the related CIS ability has not been characterized. The understanding of the regulatory mechanisms that control
StInvInh2
transcription is unclear. In this study, we identified an ERF‐VII transcription factor,
StRAP2.3
, that directly regulates
StInvInh2
to positively modulate CIS resistance. Acting as a nuclear-localized transcriptional activator, StRAP2.3 directly binds the ACCGAC cis-element in the promoter region of
StInvInh2
, enabling promoter activity. Overexpression of
StRAP2.3
in CIS-sensitive potato tubers induced
StInvInh2
mRNA abundance and increased CIS resistance. In contrast, silencing
StRAP2.3
in CIS-resistant potato tubers repressed the expression of
StInvInh2
and decreased CIS resistance. We conclude that cold-responsive
StInvInh2
is due to the binding of StRAP2.3 to the ACCGAC cis-element in the promoter region of
StInvInh2
. Overall, these findings indicate that StRAP2.3 directly regulates
StInvInh2
to positively modulate CIS resistance, which may provide a strategy to improve the processing quality of potatoes. |
| doi_str_mv | 10.1038/s41438-021-00522-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8012585</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2507356619</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-8bc485a494ef98c24a56bc7a4f5dde8e620bb7308d14774b67d1110e29f318623</originalsourceid><addsrcrecordid>eNp9kc9uFDEMxkcIRKvSF-CAInHtlPxP5oJUVS2MVAnUAtcok3h2U22TJcku6g3egGfkSQjdUuDCybb882dbX9c9J_iYYKZfFU440z2mpMdYUNqTR90-bVmvqJKPWy4l7aUmeK87LOUaY0wEp0yop90eY2rAVA773berennynh6zI2QjOrs8__H1-6dxRDXbWFwO6xpSRLN1NeUj5EMGV1e3qNVhaysUdFXHuB3jkqKaEMSljQ6QSyvfh-g3DjwqXwAqxBAXKEMJpd4hIaJ1qramZ92T2a4KHN7Hg-7j-dmH07f9xbs34-nJRe-44rXXk-NaWD5wmAftKLdCTk5ZPgvvQYOkeJoUw9oTrhSfpPKEEAx0mBnRkrKD7vVOd72ZbsA7iO3HlVnncGPzrUk2mH87MSzNIm2NxoQKLZrAy3uBnD5voFRznTY5tpsNFVgxISUZGkV3lMuplAzzwwaCzS_nzM4505wzd84Z0oZe_H3bw8hvnxrAdkBprbiA_Gf3f2R_AoAEpbk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2507356619</pqid></control><display><type>article</type><title>StRAP2.3, an ERF‐VII transcription factor, directly activates StInvInh2 to enhance cold-induced sweetening resistance in potato</title><source>Oxford Journals Open Access Collection</source><source>Nature Free</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Springer Nature OA Free Journals</source><creator>Shi, Weiling ; Song, Yuhao ; Liu, Tiantian ; Ma, Qiuqin ; Yin, Wang ; Shen, Yuchen ; Liu, Tengfei ; Jiang, Chunyan ; Zhang, Kai ; Lv, Dianqiu ; Song, Botao ; Wang, Jichun ; Liu, Xun</creator><creatorcontrib>Shi, Weiling ; Song, Yuhao ; Liu, Tiantian ; Ma, Qiuqin ; Yin, Wang ; Shen, Yuchen ; Liu, Tengfei ; Jiang, Chunyan ; Zhang, Kai ; Lv, Dianqiu ; Song, Botao ; Wang, Jichun ; Liu, Xun</creatorcontrib><description>Potato invertase inhibitor (
StInvInh2
) positively regulates cold-induced sweetening (CIS) resistance by inhibiting the activity of vacuolar invertase. The distinct expression patterns of
StInvInh2
have been thoroughly characterized in different potato genotypes, but the related CIS ability has not been characterized. The understanding of the regulatory mechanisms that control
StInvInh2
transcription is unclear. In this study, we identified an ERF‐VII transcription factor,
StRAP2.3
, that directly regulates
StInvInh2
to positively modulate CIS resistance. Acting as a nuclear-localized transcriptional activator, StRAP2.3 directly binds the ACCGAC cis-element in the promoter region of
StInvInh2
, enabling promoter activity. Overexpression of
StRAP2.3
in CIS-sensitive potato tubers induced
StInvInh2
mRNA abundance and increased CIS resistance. In contrast, silencing
StRAP2.3
in CIS-resistant potato tubers repressed the expression of
StInvInh2
and decreased CIS resistance. We conclude that cold-responsive
StInvInh2
is due to the binding of StRAP2.3 to the ACCGAC cis-element in the promoter region of
StInvInh2
. Overall, these findings indicate that StRAP2.3 directly regulates
StInvInh2
to positively modulate CIS resistance, which may provide a strategy to improve the processing quality of potatoes.</description><identifier>ISSN: 2662-6810</identifier><identifier>EISSN: 2052-7276</identifier><identifier>DOI: 10.1038/s41438-021-00522-1</identifier><identifier>PMID: 33790269</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/337 ; 631/449 ; Agriculture ; Biomedical and Life Sciences ; Ecology ; Genotypes ; Invertase ; Life Sciences ; Low temperature resistance ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant Sciences ; Potatoes ; Regulatory mechanisms (biology) ; Transcription factors ; Tubers ; Vegetables</subject><ispartof>Horticulture research, 2021-04, Vol.8 (1), p.82, Article 82</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-8bc485a494ef98c24a56bc7a4f5dde8e620bb7308d14774b67d1110e29f318623</citedby><cites>FETCH-LOGICAL-c474t-8bc485a494ef98c24a56bc7a4f5dde8e620bb7308d14774b67d1110e29f318623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012585/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012585/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33790269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Weiling</creatorcontrib><creatorcontrib>Song, Yuhao</creatorcontrib><creatorcontrib>Liu, Tiantian</creatorcontrib><creatorcontrib>Ma, Qiuqin</creatorcontrib><creatorcontrib>Yin, Wang</creatorcontrib><creatorcontrib>Shen, Yuchen</creatorcontrib><creatorcontrib>Liu, Tengfei</creatorcontrib><creatorcontrib>Jiang, Chunyan</creatorcontrib><creatorcontrib>Zhang, Kai</creatorcontrib><creatorcontrib>Lv, Dianqiu</creatorcontrib><creatorcontrib>Song, Botao</creatorcontrib><creatorcontrib>Wang, Jichun</creatorcontrib><creatorcontrib>Liu, Xun</creatorcontrib><title>StRAP2.3, an ERF‐VII transcription factor, directly activates StInvInh2 to enhance cold-induced sweetening resistance in potato</title><title>Horticulture research</title><addtitle>Hortic Res</addtitle><addtitle>Hortic Res</addtitle><description>Potato invertase inhibitor (
StInvInh2
) positively regulates cold-induced sweetening (CIS) resistance by inhibiting the activity of vacuolar invertase. The distinct expression patterns of
StInvInh2
have been thoroughly characterized in different potato genotypes, but the related CIS ability has not been characterized. The understanding of the regulatory mechanisms that control
StInvInh2
transcription is unclear. In this study, we identified an ERF‐VII transcription factor,
StRAP2.3
, that directly regulates
StInvInh2
to positively modulate CIS resistance. Acting as a nuclear-localized transcriptional activator, StRAP2.3 directly binds the ACCGAC cis-element in the promoter region of
StInvInh2
, enabling promoter activity. Overexpression of
StRAP2.3
in CIS-sensitive potato tubers induced
StInvInh2
mRNA abundance and increased CIS resistance. In contrast, silencing
StRAP2.3
in CIS-resistant potato tubers repressed the expression of
StInvInh2
and decreased CIS resistance. We conclude that cold-responsive
StInvInh2
is due to the binding of StRAP2.3 to the ACCGAC cis-element in the promoter region of
StInvInh2
. Overall, these findings indicate that StRAP2.3 directly regulates
StInvInh2
to positively modulate CIS resistance, which may provide a strategy to improve the processing quality of potatoes.</description><subject>631/337</subject><subject>631/449</subject><subject>Agriculture</subject><subject>Biomedical and Life Sciences</subject><subject>Ecology</subject><subject>Genotypes</subject><subject>Invertase</subject><subject>Life Sciences</subject><subject>Low temperature resistance</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Sciences</subject><subject>Potatoes</subject><subject>Regulatory mechanisms (biology)</subject><subject>Transcription factors</subject><subject>Tubers</subject><subject>Vegetables</subject><issn>2662-6810</issn><issn>2052-7276</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc9uFDEMxkcIRKvSF-CAInHtlPxP5oJUVS2MVAnUAtcok3h2U22TJcku6g3egGfkSQjdUuDCybb882dbX9c9J_iYYKZfFU440z2mpMdYUNqTR90-bVmvqJKPWy4l7aUmeK87LOUaY0wEp0yop90eY2rAVA773berennynh6zI2QjOrs8__H1-6dxRDXbWFwO6xpSRLN1NeUj5EMGV1e3qNVhaysUdFXHuB3jkqKaEMSljQ6QSyvfh-g3DjwqXwAqxBAXKEMJpd4hIaJ1qramZ92T2a4KHN7Hg-7j-dmH07f9xbs34-nJRe-44rXXk-NaWD5wmAftKLdCTk5ZPgvvQYOkeJoUw9oTrhSfpPKEEAx0mBnRkrKD7vVOd72ZbsA7iO3HlVnncGPzrUk2mH87MSzNIm2NxoQKLZrAy3uBnD5voFRznTY5tpsNFVgxISUZGkV3lMuplAzzwwaCzS_nzM4505wzd84Z0oZe_H3bw8hvnxrAdkBprbiA_Gf3f2R_AoAEpbk</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Shi, Weiling</creator><creator>Song, Yuhao</creator><creator>Liu, Tiantian</creator><creator>Ma, Qiuqin</creator><creator>Yin, Wang</creator><creator>Shen, Yuchen</creator><creator>Liu, Tengfei</creator><creator>Jiang, Chunyan</creator><creator>Zhang, Kai</creator><creator>Lv, Dianqiu</creator><creator>Song, Botao</creator><creator>Wang, Jichun</creator><creator>Liu, Xun</creator><general>Nature Publishing Group UK</general><general>Oxford University Press</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20210401</creationdate><title>StRAP2.3, an ERF‐VII transcription factor, directly activates StInvInh2 to enhance cold-induced sweetening resistance in potato</title><author>Shi, Weiling ; 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StInvInh2
) positively regulates cold-induced sweetening (CIS) resistance by inhibiting the activity of vacuolar invertase. The distinct expression patterns of
StInvInh2
have been thoroughly characterized in different potato genotypes, but the related CIS ability has not been characterized. The understanding of the regulatory mechanisms that control
StInvInh2
transcription is unclear. In this study, we identified an ERF‐VII transcription factor,
StRAP2.3
, that directly regulates
StInvInh2
to positively modulate CIS resistance. Acting as a nuclear-localized transcriptional activator, StRAP2.3 directly binds the ACCGAC cis-element in the promoter region of
StInvInh2
, enabling promoter activity. Overexpression of
StRAP2.3
in CIS-sensitive potato tubers induced
StInvInh2
mRNA abundance and increased CIS resistance. In contrast, silencing
StRAP2.3
in CIS-resistant potato tubers repressed the expression of
StInvInh2
and decreased CIS resistance. We conclude that cold-responsive
StInvInh2
is due to the binding of StRAP2.3 to the ACCGAC cis-element in the promoter region of
StInvInh2
. Overall, these findings indicate that StRAP2.3 directly regulates
StInvInh2
to positively modulate CIS resistance, which may provide a strategy to improve the processing quality of potatoes.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33790269</pmid><doi>10.1038/s41438-021-00522-1</doi><oa>free_for_read</oa></addata></record> |
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| source | Oxford Journals Open Access Collection; Nature Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Springer Nature OA Free Journals |
| subjects | 631/337 631/449 Agriculture Biomedical and Life Sciences Ecology Genotypes Invertase Life Sciences Low temperature resistance Plant Breeding/Biotechnology Plant Genetics and Genomics Plant Sciences Potatoes Regulatory mechanisms (biology) Transcription factors Tubers Vegetables |
| title | StRAP2.3, an ERF‐VII transcription factor, directly activates StInvInh2 to enhance cold-induced sweetening resistance in potato |
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