Alterations in T and B cell function persist in convalescent COVID-19 patients
Emerging studies indicate that some coronavirus disease 2019 (COVID-19) patients suffer from persistent symptoms, including breathlessness and chronic fatigue; however, the long-term immune response in these patients presently remains ill-defined. Here, we describe the phenotypic and functional char...
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| Veröffentlicht in: | Med (New York, N.Y. : Online) N.Y. : Online), 2021-06, Vol.2 (6), p.720-735.e4 |
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| creator | Shuwa, Halima A. Shaw, Tovah N. Knight, Sean B. Wemyss, Kelly McClure, Flora A. Pearmain, Laurence Prise, Ian Jagger, Christopher Morgan, David J. Khan, Saba Brand, Oliver Mann, Elizabeth R. Ustianowski, Andrew Bakerly, Nawar Diar Dark, Paul Brightling, Christopher E. Brij, Seema Ahmed, Rohan Avery, Miriam Birchall, Katharine Charsley, Evelyn Chenery, Alistair Chew, Christine Clark, Richard Connolly, Emma Connolly, Karen Dawson, Simon Durrans, Laura Durrington, Hannah Egan, Jasmine Filbey, Kara Fox, Claire Francis, Helen Franklin, Miriam Glasgow, Susannah Godfrey, Nicola Gray, Kathryn J. Grundy, Seamus Guerin, Jacinta Hackney, Pamela Hayes, Chantelle Hardy, Emma Harris, Jade John, Anu Jolly, Bethany Kästele, Verena Kerry, Gina Lui, Sylvia Lin, Lijing Mathioudakis, Alex G. Mitchell, Joanne Moizer, Clare Moore, Katrina Moss, Stuart Baker, Syed Murtuza Oliver, Rob Padden, Grace Parkinson, Christina Phuycharoen, Michael Saha, Ananya Salcman, Barbora Scott, Nicholas A. Sharma, Seema Shaw, Jane Shaw, Joanne Shepley, Elizabeth Smith, Lara Stephan, Simon Stephens, Ruth Tavernier, Gael Tudge, Rhys Wareing, Louis Warren, Roanna Williams, Thomas Willmore, Lisa Younas, Mehwish Felton, Timothy Simpson, Angela Grainger, John R. Hussell, Tracy Konkel, Joanne E. Menon, Madhvi |
| description | Emerging studies indicate that some coronavirus disease 2019 (COVID-19) patients suffer from persistent symptoms, including breathlessness and chronic fatigue; however, the long-term immune response in these patients presently remains ill-defined.
Here, we describe the phenotypic and functional characteristics of B and T cells in hospitalized COVID-19 patients during acute disease and at 3–6 months of convalescence.
We report that the alterations in B cell subsets observed in acute COVID-19 patients were largely recovered in convalescent patients. In contrast, T cells from convalescent patients displayed continued alterations with persistence of a cytotoxic program evident in CD8+ T cells as well as elevated production of type 1 cytokines and interleukin-17 (IL-17). Interestingly, B cells from patients with acute COVID-19 displayed an IL-6/IL-10 cytokine imbalance in response to Toll-like receptor activation, skewed toward a pro-inflammatory phenotype. Whereas the frequency of IL-6+ B cells was restored in convalescent patients irrespective of clinical outcome, the recovery of IL-10+ B cells was associated with the resolution of lung pathology.
Our data detail lymphocyte alterations in previously hospitalized COVID-19 patients up to 6 months following hospital discharge and identify 3 subgroups of convalescent patients based on distinct lymphocyte phenotypes, with 1 subgroup associated with poorer clinical outcome. We propose that alterations in B and T cell function following hospitalization with COVID-19 could affect longer-term immunity and contribute to some persistent symptoms observed in convalescent COVID-19 patients.
Provided by UKRI, Lister Institute of Preventative Medicine, the Wellcome Trust, The Kennedy Trust for Rheumatology Research, and 3M Global Giving.
[Display omitted]
Lymphocytes were examined during COVID-19 and at up to 6 months of convalescenceB cell changes seen during acute COVID-19 were largely restored in convalescenceT cells from convalescent COVID-19 patients displayed persistent changesLymphocyte signatures defined 3 convalescent patient groups, one with poorer outcomes
The coronavirus disease 2019 (COVID-19) pandemic, caused by a novel coronavirus strain, has resulted in >100 million infections worldwide. Emerging evidence suggests that some COVID-19 patients suffer persistent symptoms, including fatigue, fibrotic lung disease, and myalgia; however, the long-term immune response in these patients remains ill-defined. Here, we |
| doi_str_mv | 10.1016/j.medj.2021.03.013 |
| format | Article |
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Here, we describe the phenotypic and functional characteristics of B and T cells in hospitalized COVID-19 patients during acute disease and at 3–6 months of convalescence.
We report that the alterations in B cell subsets observed in acute COVID-19 patients were largely recovered in convalescent patients. In contrast, T cells from convalescent patients displayed continued alterations with persistence of a cytotoxic program evident in CD8+ T cells as well as elevated production of type 1 cytokines and interleukin-17 (IL-17). Interestingly, B cells from patients with acute COVID-19 displayed an IL-6/IL-10 cytokine imbalance in response to Toll-like receptor activation, skewed toward a pro-inflammatory phenotype. Whereas the frequency of IL-6+ B cells was restored in convalescent patients irrespective of clinical outcome, the recovery of IL-10+ B cells was associated with the resolution of lung pathology.
Our data detail lymphocyte alterations in previously hospitalized COVID-19 patients up to 6 months following hospital discharge and identify 3 subgroups of convalescent patients based on distinct lymphocyte phenotypes, with 1 subgroup associated with poorer clinical outcome. We propose that alterations in B and T cell function following hospitalization with COVID-19 could affect longer-term immunity and contribute to some persistent symptoms observed in convalescent COVID-19 patients.
Provided by UKRI, Lister Institute of Preventative Medicine, the Wellcome Trust, The Kennedy Trust for Rheumatology Research, and 3M Global Giving.
[Display omitted]
Lymphocytes were examined during COVID-19 and at up to 6 months of convalescenceB cell changes seen during acute COVID-19 were largely restored in convalescenceT cells from convalescent COVID-19 patients displayed persistent changesLymphocyte signatures defined 3 convalescent patient groups, one with poorer outcomes
The coronavirus disease 2019 (COVID-19) pandemic, caused by a novel coronavirus strain, has resulted in >100 million infections worldwide. Emerging evidence suggests that some COVID-19 patients suffer persistent symptoms, including fatigue, fibrotic lung disease, and myalgia; however, the long-term immune response in these patients remains ill-defined. Here, we conducted an observational study examining lymphocyte populations in COVID-19 patients during hospitalization and at up to 6 months of convalescence. We identified a number of lymphocyte alterations that persisted in convalescent patients. Moreover, the compilation of lymphocyte parameters in convalescent COVID-19 patients identified 3 distinct patient subgroups, with 1 subgroup associated with poorer clinical outcome. Our study outlines lymphocyte changes in convalescent COVID-19 patients associated with negative effects on subsequent health.
Shuwa et al. examine lymphocyte characteristics in acute and convalescent COVID-19 patients, detailing persistent alterations in lymphocyte phenotype up to 6 months following hospital discharge. In this report, they identify 3 subgroups of convalescent patients based on distinct lymphocyte signatures, with 1 subgroup associated with poorer clinical outcomes.</description><identifier>ISSN: 2666-6340</identifier><identifier>ISSN: 2666-6359</identifier><identifier>EISSN: 2666-6340</identifier><identifier>DOI: 10.1016/j.medj.2021.03.013</identifier><identifier>PMID: 33821250</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>B cells ; CD8-Positive T-Lymphocytes ; Clinical and Translational Report ; convalescent patients ; COVID-19 ; Cytokines ; Humans ; Interleukin-10 ; Interleukin-6 ; long COVID ; SARS-CoV-2 ; T cells ; viral Infection</subject><ispartof>Med (New York, N.Y. : Online), 2021-06, Vol.2 (6), p.720-735.e4</ispartof><rights>2021 The Author(s)</rights><rights>2021 The Author(s).</rights><rights>2021 The Author(s) 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-afc725d3ece8d4364379fd19ec602540069103e6056f2c848367638c103ba22a3</citedby><cites>FETCH-LOGICAL-c455t-afc725d3ece8d4364379fd19ec602540069103e6056f2c848367638c103ba22a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33821250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shuwa, Halima A.</creatorcontrib><creatorcontrib>Shaw, Tovah N.</creatorcontrib><creatorcontrib>Knight, Sean B.</creatorcontrib><creatorcontrib>Wemyss, Kelly</creatorcontrib><creatorcontrib>McClure, Flora A.</creatorcontrib><creatorcontrib>Pearmain, Laurence</creatorcontrib><creatorcontrib>Prise, Ian</creatorcontrib><creatorcontrib>Jagger, Christopher</creatorcontrib><creatorcontrib>Morgan, David J.</creatorcontrib><creatorcontrib>Khan, Saba</creatorcontrib><creatorcontrib>Brand, Oliver</creatorcontrib><creatorcontrib>Mann, Elizabeth R.</creatorcontrib><creatorcontrib>Ustianowski, Andrew</creatorcontrib><creatorcontrib>Bakerly, Nawar Diar</creatorcontrib><creatorcontrib>Dark, Paul</creatorcontrib><creatorcontrib>Brightling, Christopher E.</creatorcontrib><creatorcontrib>Brij, Seema</creatorcontrib><creatorcontrib>Ahmed, Rohan</creatorcontrib><creatorcontrib>Avery, Miriam</creatorcontrib><creatorcontrib>Birchall, Katharine</creatorcontrib><creatorcontrib>Charsley, Evelyn</creatorcontrib><creatorcontrib>Chenery, Alistair</creatorcontrib><creatorcontrib>Chew, Christine</creatorcontrib><creatorcontrib>Clark, Richard</creatorcontrib><creatorcontrib>Connolly, Emma</creatorcontrib><creatorcontrib>Connolly, Karen</creatorcontrib><creatorcontrib>Dawson, Simon</creatorcontrib><creatorcontrib>Durrans, Laura</creatorcontrib><creatorcontrib>Durrington, Hannah</creatorcontrib><creatorcontrib>Egan, Jasmine</creatorcontrib><creatorcontrib>Filbey, Kara</creatorcontrib><creatorcontrib>Fox, Claire</creatorcontrib><creatorcontrib>Francis, Helen</creatorcontrib><creatorcontrib>Franklin, Miriam</creatorcontrib><creatorcontrib>Glasgow, Susannah</creatorcontrib><creatorcontrib>Godfrey, Nicola</creatorcontrib><creatorcontrib>Gray, Kathryn J.</creatorcontrib><creatorcontrib>Grundy, Seamus</creatorcontrib><creatorcontrib>Guerin, Jacinta</creatorcontrib><creatorcontrib>Hackney, Pamela</creatorcontrib><creatorcontrib>Hayes, Chantelle</creatorcontrib><creatorcontrib>Hardy, Emma</creatorcontrib><creatorcontrib>Harris, Jade</creatorcontrib><creatorcontrib>John, Anu</creatorcontrib><creatorcontrib>Jolly, Bethany</creatorcontrib><creatorcontrib>Kästele, Verena</creatorcontrib><creatorcontrib>Kerry, Gina</creatorcontrib><creatorcontrib>Lui, Sylvia</creatorcontrib><creatorcontrib>Lin, Lijing</creatorcontrib><creatorcontrib>Mathioudakis, Alex G.</creatorcontrib><creatorcontrib>Mitchell, Joanne</creatorcontrib><creatorcontrib>Moizer, Clare</creatorcontrib><creatorcontrib>Moore, Katrina</creatorcontrib><creatorcontrib>Moss, Stuart</creatorcontrib><creatorcontrib>Baker, Syed Murtuza</creatorcontrib><creatorcontrib>Oliver, Rob</creatorcontrib><creatorcontrib>Padden, Grace</creatorcontrib><creatorcontrib>Parkinson, Christina</creatorcontrib><creatorcontrib>Phuycharoen, Michael</creatorcontrib><creatorcontrib>Saha, Ananya</creatorcontrib><creatorcontrib>Salcman, Barbora</creatorcontrib><creatorcontrib>Scott, Nicholas A.</creatorcontrib><creatorcontrib>Sharma, Seema</creatorcontrib><creatorcontrib>Shaw, Jane</creatorcontrib><creatorcontrib>Shaw, Joanne</creatorcontrib><creatorcontrib>Shepley, Elizabeth</creatorcontrib><creatorcontrib>Smith, Lara</creatorcontrib><creatorcontrib>Stephan, Simon</creatorcontrib><creatorcontrib>Stephens, Ruth</creatorcontrib><creatorcontrib>Tavernier, Gael</creatorcontrib><creatorcontrib>Tudge, Rhys</creatorcontrib><creatorcontrib>Wareing, Louis</creatorcontrib><creatorcontrib>Warren, Roanna</creatorcontrib><creatorcontrib>Williams, Thomas</creatorcontrib><creatorcontrib>Willmore, Lisa</creatorcontrib><creatorcontrib>Younas, Mehwish</creatorcontrib><creatorcontrib>Felton, Timothy</creatorcontrib><creatorcontrib>Simpson, Angela</creatorcontrib><creatorcontrib>Grainger, John R.</creatorcontrib><creatorcontrib>Hussell, Tracy</creatorcontrib><creatorcontrib>Konkel, Joanne E.</creatorcontrib><creatorcontrib>Menon, Madhvi</creatorcontrib><creatorcontrib>CIRCO</creatorcontrib><title>Alterations in T and B cell function persist in convalescent COVID-19 patients</title><title>Med (New York, N.Y. : Online)</title><addtitle>Med (N Y)</addtitle><description>Emerging studies indicate that some coronavirus disease 2019 (COVID-19) patients suffer from persistent symptoms, including breathlessness and chronic fatigue; however, the long-term immune response in these patients presently remains ill-defined.
Here, we describe the phenotypic and functional characteristics of B and T cells in hospitalized COVID-19 patients during acute disease and at 3–6 months of convalescence.
We report that the alterations in B cell subsets observed in acute COVID-19 patients were largely recovered in convalescent patients. In contrast, T cells from convalescent patients displayed continued alterations with persistence of a cytotoxic program evident in CD8+ T cells as well as elevated production of type 1 cytokines and interleukin-17 (IL-17). Interestingly, B cells from patients with acute COVID-19 displayed an IL-6/IL-10 cytokine imbalance in response to Toll-like receptor activation, skewed toward a pro-inflammatory phenotype. Whereas the frequency of IL-6+ B cells was restored in convalescent patients irrespective of clinical outcome, the recovery of IL-10+ B cells was associated with the resolution of lung pathology.
Our data detail lymphocyte alterations in previously hospitalized COVID-19 patients up to 6 months following hospital discharge and identify 3 subgroups of convalescent patients based on distinct lymphocyte phenotypes, with 1 subgroup associated with poorer clinical outcome. We propose that alterations in B and T cell function following hospitalization with COVID-19 could affect longer-term immunity and contribute to some persistent symptoms observed in convalescent COVID-19 patients.
Provided by UKRI, Lister Institute of Preventative Medicine, the Wellcome Trust, The Kennedy Trust for Rheumatology Research, and 3M Global Giving.
[Display omitted]
Lymphocytes were examined during COVID-19 and at up to 6 months of convalescenceB cell changes seen during acute COVID-19 were largely restored in convalescenceT cells from convalescent COVID-19 patients displayed persistent changesLymphocyte signatures defined 3 convalescent patient groups, one with poorer outcomes
The coronavirus disease 2019 (COVID-19) pandemic, caused by a novel coronavirus strain, has resulted in >100 million infections worldwide. Emerging evidence suggests that some COVID-19 patients suffer persistent symptoms, including fatigue, fibrotic lung disease, and myalgia; however, the long-term immune response in these patients remains ill-defined. Here, we conducted an observational study examining lymphocyte populations in COVID-19 patients during hospitalization and at up to 6 months of convalescence. We identified a number of lymphocyte alterations that persisted in convalescent patients. Moreover, the compilation of lymphocyte parameters in convalescent COVID-19 patients identified 3 distinct patient subgroups, with 1 subgroup associated with poorer clinical outcome. Our study outlines lymphocyte changes in convalescent COVID-19 patients associated with negative effects on subsequent health.
Shuwa et al. examine lymphocyte characteristics in acute and convalescent COVID-19 patients, detailing persistent alterations in lymphocyte phenotype up to 6 months following hospital discharge. In this report, they identify 3 subgroups of convalescent patients based on distinct lymphocyte signatures, with 1 subgroup associated with poorer clinical outcomes.</description><subject>B cells</subject><subject>CD8-Positive T-Lymphocytes</subject><subject>Clinical and Translational Report</subject><subject>convalescent patients</subject><subject>COVID-19</subject><subject>Cytokines</subject><subject>Humans</subject><subject>Interleukin-10</subject><subject>Interleukin-6</subject><subject>long COVID</subject><subject>SARS-CoV-2</subject><subject>T cells</subject><subject>viral Infection</subject><issn>2666-6340</issn><issn>2666-6359</issn><issn>2666-6340</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1LxDAQDaKorP4BD5Kjl9ZJ0qYtiKDr14K4l9VriOlUU7rtmrQL_ntTVhe9eJph5s3Ly3uEnDCIGTB5XsdLLOuYA2cxiBiY2CGHXEoZSZHA7q_-gBx7XwMAT5lgBd8nB0LknPEUDsnTVdOj073tWk9tSxdUtyW9pgabhlZDa8YNXaHz1vcjwHTtWjfoDbY9nc5fZjcRK-gqMISBPyJ7lW48Hn_XCXm-u11MH6LH-f1sevUYmSRN-0hXJuNpKdBgXiZCJiIrqpIVaGRQmQDIgoFACamsuMmTXMhMityE4avmXIsJudzwrobX4MMoxulGrZxdavepOm3V301r39Vbt1Y5MCbzIhCcfRO47mNA36ul9eOndYvd4FUwp-AZ54kIUL6BGtd577DaPsNAjVmoWo1ZqDELBUKFLMLR6W-B25Mf5wPgYgPAYNPaolPeBAsNltah6VXZ2f_4vwDiQZlD</recordid><startdate>20210611</startdate><enddate>20210611</enddate><creator>Shuwa, Halima A.</creator><creator>Shaw, Tovah N.</creator><creator>Knight, Sean B.</creator><creator>Wemyss, Kelly</creator><creator>McClure, Flora A.</creator><creator>Pearmain, Laurence</creator><creator>Prise, 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Elizabeth</creator><creator>Smith, Lara</creator><creator>Stephan, Simon</creator><creator>Stephens, Ruth</creator><creator>Tavernier, Gael</creator><creator>Tudge, Rhys</creator><creator>Wareing, Louis</creator><creator>Warren, Roanna</creator><creator>Williams, Thomas</creator><creator>Willmore, Lisa</creator><creator>Younas, Mehwish</creator><creator>Felton, Timothy</creator><creator>Simpson, Angela</creator><creator>Grainger, John R.</creator><creator>Hussell, Tracy</creator><creator>Konkel, Joanne E.</creator><creator>Menon, Madhvi</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20210611</creationdate><title>Alterations in T and B cell function persist in convalescent COVID-19 patients</title><author>Shuwa, Halima A. ; Shaw, Tovah N. ; Knight, Sean B. ; Wemyss, Kelly ; McClure, Flora A. ; Pearmain, Laurence ; Prise, Ian ; Jagger, Christopher ; Morgan, David J. ; Khan, Saba ; Brand, Oliver ; Mann, Elizabeth R. ; Ustianowski, Andrew ; Bakerly, Nawar Diar ; Dark, Paul ; Brightling, Christopher E. ; Brij, Seema ; Ahmed, Rohan ; Avery, Miriam ; Birchall, Katharine ; Charsley, Evelyn ; Chenery, Alistair ; Chew, Christine ; Clark, Richard ; Connolly, Emma ; Connolly, Karen ; Dawson, Simon ; Durrans, Laura ; Durrington, Hannah ; Egan, Jasmine ; Filbey, Kara ; Fox, Claire ; Francis, Helen ; Franklin, Miriam ; Glasgow, Susannah ; Godfrey, Nicola ; Gray, Kathryn J. ; Grundy, Seamus ; Guerin, Jacinta ; Hackney, Pamela ; Hayes, Chantelle ; Hardy, Emma ; Harris, Jade ; John, Anu ; Jolly, Bethany ; Kästele, Verena ; Kerry, Gina ; Lui, Sylvia ; Lin, Lijing ; Mathioudakis, Alex G. ; Mitchell, Joanne ; Moizer, Clare ; Moore, Katrina ; Moss, Stuart ; Baker, Syed Murtuza ; Oliver, Rob ; Padden, Grace ; Parkinson, Christina ; Phuycharoen, Michael ; Saha, Ananya ; Salcman, Barbora ; Scott, Nicholas A. ; Sharma, Seema ; Shaw, Jane ; Shaw, Joanne ; Shepley, Elizabeth ; Smith, Lara ; Stephan, Simon ; Stephens, Ruth ; Tavernier, Gael ; Tudge, Rhys ; Wareing, Louis ; Warren, Roanna ; Williams, Thomas ; Willmore, Lisa ; Younas, Mehwish ; Felton, Timothy ; Simpson, Angela ; Grainger, John R. ; Hussell, Tracy ; Konkel, Joanne E. ; Menon, Madhvi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-afc725d3ece8d4364379fd19ec602540069103e6056f2c848367638c103ba22a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>B cells</topic><topic>CD8-Positive T-Lymphocytes</topic><topic>Clinical and Translational Report</topic><topic>convalescent patients</topic><topic>COVID-19</topic><topic>Cytokines</topic><topic>Humans</topic><topic>Interleukin-10</topic><topic>Interleukin-6</topic><topic>long COVID</topic><topic>SARS-CoV-2</topic><topic>T cells</topic><topic>viral Infection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shuwa, Halima A.</creatorcontrib><creatorcontrib>Shaw, Tovah N.</creatorcontrib><creatorcontrib>Knight, Sean B.</creatorcontrib><creatorcontrib>Wemyss, Kelly</creatorcontrib><creatorcontrib>McClure, Flora A.</creatorcontrib><creatorcontrib>Pearmain, Laurence</creatorcontrib><creatorcontrib>Prise, Ian</creatorcontrib><creatorcontrib>Jagger, Christopher</creatorcontrib><creatorcontrib>Morgan, David J.</creatorcontrib><creatorcontrib>Khan, Saba</creatorcontrib><creatorcontrib>Brand, Oliver</creatorcontrib><creatorcontrib>Mann, Elizabeth R.</creatorcontrib><creatorcontrib>Ustianowski, Andrew</creatorcontrib><creatorcontrib>Bakerly, Nawar Diar</creatorcontrib><creatorcontrib>Dark, Paul</creatorcontrib><creatorcontrib>Brightling, Christopher E.</creatorcontrib><creatorcontrib>Brij, Seema</creatorcontrib><creatorcontrib>Ahmed, Rohan</creatorcontrib><creatorcontrib>Avery, Miriam</creatorcontrib><creatorcontrib>Birchall, Katharine</creatorcontrib><creatorcontrib>Charsley, Evelyn</creatorcontrib><creatorcontrib>Chenery, Alistair</creatorcontrib><creatorcontrib>Chew, Christine</creatorcontrib><creatorcontrib>Clark, Richard</creatorcontrib><creatorcontrib>Connolly, Emma</creatorcontrib><creatorcontrib>Connolly, Karen</creatorcontrib><creatorcontrib>Dawson, Simon</creatorcontrib><creatorcontrib>Durrans, Laura</creatorcontrib><creatorcontrib>Durrington, Hannah</creatorcontrib><creatorcontrib>Egan, Jasmine</creatorcontrib><creatorcontrib>Filbey, Kara</creatorcontrib><creatorcontrib>Fox, Claire</creatorcontrib><creatorcontrib>Francis, Helen</creatorcontrib><creatorcontrib>Franklin, Miriam</creatorcontrib><creatorcontrib>Glasgow, Susannah</creatorcontrib><creatorcontrib>Godfrey, Nicola</creatorcontrib><creatorcontrib>Gray, Kathryn J.</creatorcontrib><creatorcontrib>Grundy, Seamus</creatorcontrib><creatorcontrib>Guerin, Jacinta</creatorcontrib><creatorcontrib>Hackney, Pamela</creatorcontrib><creatorcontrib>Hayes, Chantelle</creatorcontrib><creatorcontrib>Hardy, Emma</creatorcontrib><creatorcontrib>Harris, Jade</creatorcontrib><creatorcontrib>John, Anu</creatorcontrib><creatorcontrib>Jolly, Bethany</creatorcontrib><creatorcontrib>Kästele, Verena</creatorcontrib><creatorcontrib>Kerry, Gina</creatorcontrib><creatorcontrib>Lui, Sylvia</creatorcontrib><creatorcontrib>Lin, Lijing</creatorcontrib><creatorcontrib>Mathioudakis, Alex G.</creatorcontrib><creatorcontrib>Mitchell, Joanne</creatorcontrib><creatorcontrib>Moizer, Clare</creatorcontrib><creatorcontrib>Moore, Katrina</creatorcontrib><creatorcontrib>Moss, Stuart</creatorcontrib><creatorcontrib>Baker, Syed Murtuza</creatorcontrib><creatorcontrib>Oliver, Rob</creatorcontrib><creatorcontrib>Padden, Grace</creatorcontrib><creatorcontrib>Parkinson, Christina</creatorcontrib><creatorcontrib>Phuycharoen, Michael</creatorcontrib><creatorcontrib>Saha, Ananya</creatorcontrib><creatorcontrib>Salcman, Barbora</creatorcontrib><creatorcontrib>Scott, Nicholas A.</creatorcontrib><creatorcontrib>Sharma, Seema</creatorcontrib><creatorcontrib>Shaw, Jane</creatorcontrib><creatorcontrib>Shaw, Joanne</creatorcontrib><creatorcontrib>Shepley, Elizabeth</creatorcontrib><creatorcontrib>Smith, Lara</creatorcontrib><creatorcontrib>Stephan, Simon</creatorcontrib><creatorcontrib>Stephens, Ruth</creatorcontrib><creatorcontrib>Tavernier, Gael</creatorcontrib><creatorcontrib>Tudge, Rhys</creatorcontrib><creatorcontrib>Wareing, Louis</creatorcontrib><creatorcontrib>Warren, Roanna</creatorcontrib><creatorcontrib>Williams, Thomas</creatorcontrib><creatorcontrib>Willmore, Lisa</creatorcontrib><creatorcontrib>Younas, Mehwish</creatorcontrib><creatorcontrib>Felton, Timothy</creatorcontrib><creatorcontrib>Simpson, Angela</creatorcontrib><creatorcontrib>Grainger, John R.</creatorcontrib><creatorcontrib>Hussell, Tracy</creatorcontrib><creatorcontrib>Konkel, Joanne E.</creatorcontrib><creatorcontrib>Menon, Madhvi</creatorcontrib><creatorcontrib>CIRCO</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Med (New York, N.Y. : Online)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shuwa, Halima A.</au><au>Shaw, Tovah N.</au><au>Knight, Sean B.</au><au>Wemyss, Kelly</au><au>McClure, Flora A.</au><au>Pearmain, Laurence</au><au>Prise, Ian</au><au>Jagger, Christopher</au><au>Morgan, David J.</au><au>Khan, Saba</au><au>Brand, Oliver</au><au>Mann, Elizabeth R.</au><au>Ustianowski, Andrew</au><au>Bakerly, Nawar Diar</au><au>Dark, Paul</au><au>Brightling, Christopher E.</au><au>Brij, Seema</au><au>Ahmed, Rohan</au><au>Avery, Miriam</au><au>Birchall, Katharine</au><au>Charsley, Evelyn</au><au>Chenery, Alistair</au><au>Chew, Christine</au><au>Clark, Richard</au><au>Connolly, Emma</au><au>Connolly, Karen</au><au>Dawson, Simon</au><au>Durrans, Laura</au><au>Durrington, Hannah</au><au>Egan, Jasmine</au><au>Filbey, Kara</au><au>Fox, Claire</au><au>Francis, Helen</au><au>Franklin, Miriam</au><au>Glasgow, Susannah</au><au>Godfrey, Nicola</au><au>Gray, Kathryn J.</au><au>Grundy, Seamus</au><au>Guerin, Jacinta</au><au>Hackney, Pamela</au><au>Hayes, Chantelle</au><au>Hardy, Emma</au><au>Harris, Jade</au><au>John, Anu</au><au>Jolly, Bethany</au><au>Kästele, Verena</au><au>Kerry, Gina</au><au>Lui, Sylvia</au><au>Lin, Lijing</au><au>Mathioudakis, Alex G.</au><au>Mitchell, Joanne</au><au>Moizer, Clare</au><au>Moore, Katrina</au><au>Moss, Stuart</au><au>Baker, Syed Murtuza</au><au>Oliver, Rob</au><au>Padden, Grace</au><au>Parkinson, Christina</au><au>Phuycharoen, Michael</au><au>Saha, Ananya</au><au>Salcman, Barbora</au><au>Scott, Nicholas A.</au><au>Sharma, Seema</au><au>Shaw, Jane</au><au>Shaw, Joanne</au><au>Shepley, Elizabeth</au><au>Smith, Lara</au><au>Stephan, Simon</au><au>Stephens, Ruth</au><au>Tavernier, Gael</au><au>Tudge, Rhys</au><au>Wareing, Louis</au><au>Warren, Roanna</au><au>Williams, Thomas</au><au>Willmore, Lisa</au><au>Younas, Mehwish</au><au>Felton, Timothy</au><au>Simpson, Angela</au><au>Grainger, John R.</au><au>Hussell, Tracy</au><au>Konkel, Joanne E.</au><au>Menon, Madhvi</au><aucorp>CIRCO</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alterations in T and B cell function persist in convalescent COVID-19 patients</atitle><jtitle>Med (New York, N.Y. : Online)</jtitle><addtitle>Med (N Y)</addtitle><date>2021-06-11</date><risdate>2021</risdate><volume>2</volume><issue>6</issue><spage>720</spage><epage>735.e4</epage><pages>720-735.e4</pages><issn>2666-6340</issn><issn>2666-6359</issn><eissn>2666-6340</eissn><abstract>Emerging studies indicate that some coronavirus disease 2019 (COVID-19) patients suffer from persistent symptoms, including breathlessness and chronic fatigue; however, the long-term immune response in these patients presently remains ill-defined.
Here, we describe the phenotypic and functional characteristics of B and T cells in hospitalized COVID-19 patients during acute disease and at 3–6 months of convalescence.
We report that the alterations in B cell subsets observed in acute COVID-19 patients were largely recovered in convalescent patients. In contrast, T cells from convalescent patients displayed continued alterations with persistence of a cytotoxic program evident in CD8+ T cells as well as elevated production of type 1 cytokines and interleukin-17 (IL-17). Interestingly, B cells from patients with acute COVID-19 displayed an IL-6/IL-10 cytokine imbalance in response to Toll-like receptor activation, skewed toward a pro-inflammatory phenotype. Whereas the frequency of IL-6+ B cells was restored in convalescent patients irrespective of clinical outcome, the recovery of IL-10+ B cells was associated with the resolution of lung pathology.
Our data detail lymphocyte alterations in previously hospitalized COVID-19 patients up to 6 months following hospital discharge and identify 3 subgroups of convalescent patients based on distinct lymphocyte phenotypes, with 1 subgroup associated with poorer clinical outcome. We propose that alterations in B and T cell function following hospitalization with COVID-19 could affect longer-term immunity and contribute to some persistent symptoms observed in convalescent COVID-19 patients.
Provided by UKRI, Lister Institute of Preventative Medicine, the Wellcome Trust, The Kennedy Trust for Rheumatology Research, and 3M Global Giving.
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Lymphocytes were examined during COVID-19 and at up to 6 months of convalescenceB cell changes seen during acute COVID-19 were largely restored in convalescenceT cells from convalescent COVID-19 patients displayed persistent changesLymphocyte signatures defined 3 convalescent patient groups, one with poorer outcomes
The coronavirus disease 2019 (COVID-19) pandemic, caused by a novel coronavirus strain, has resulted in >100 million infections worldwide. Emerging evidence suggests that some COVID-19 patients suffer persistent symptoms, including fatigue, fibrotic lung disease, and myalgia; however, the long-term immune response in these patients remains ill-defined. Here, we conducted an observational study examining lymphocyte populations in COVID-19 patients during hospitalization and at up to 6 months of convalescence. We identified a number of lymphocyte alterations that persisted in convalescent patients. Moreover, the compilation of lymphocyte parameters in convalescent COVID-19 patients identified 3 distinct patient subgroups, with 1 subgroup associated with poorer clinical outcome. Our study outlines lymphocyte changes in convalescent COVID-19 patients associated with negative effects on subsequent health.
Shuwa et al. examine lymphocyte characteristics in acute and convalescent COVID-19 patients, detailing persistent alterations in lymphocyte phenotype up to 6 months following hospital discharge. In this report, they identify 3 subgroups of convalescent patients based on distinct lymphocyte signatures, with 1 subgroup associated with poorer clinical outcomes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>33821250</pmid><doi>10.1016/j.medj.2021.03.013</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2666-6340 |
| ispartof | Med (New York, N.Y. : Online), 2021-06, Vol.2 (6), p.720-735.e4 |
| issn | 2666-6340 2666-6359 2666-6340 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8011689 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | B cells CD8-Positive T-Lymphocytes Clinical and Translational Report convalescent patients COVID-19 Cytokines Humans Interleukin-10 Interleukin-6 long COVID SARS-CoV-2 T cells viral Infection |
| title | Alterations in T and B cell function persist in convalescent COVID-19 patients |
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