A genome-wide association study reveals cytokinin as a major component in the root defense responses against Ralstonia solanacearum

Abstract Bacterial wilt caused by the soil-borne pathogen Ralstonia solancearum is economically devastating, with no effective methods to fight the disease. This pathogen invades plants through their roots and colonizes their xylem, clogging the vasculature and causing rapid wilting. Key to preventi...

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Veröffentlicht in:Journal of experimental botany 2021-03, Vol.72 (7), p.2727-2740
Hauptverfasser: Alonso-Díaz, Alejandro, Satbhai, Santosh B, de Pedro-Jové, Roger, Berry, Hannah M, Göschl, Christian, Argueso, Cristiana T, Novak, Ondrej, Busch, Wolfgang, Valls, Marc, Coll, Núria S
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container_end_page 2740
container_issue 7
container_start_page 2727
container_title Journal of experimental botany
container_volume 72
creator Alonso-Díaz, Alejandro
Satbhai, Santosh B
de Pedro-Jové, Roger
Berry, Hannah M
Göschl, Christian
Argueso, Cristiana T
Novak, Ondrej
Busch, Wolfgang
Valls, Marc
Coll, Núria S
description Abstract Bacterial wilt caused by the soil-borne pathogen Ralstonia solancearum is economically devastating, with no effective methods to fight the disease. This pathogen invades plants through their roots and colonizes their xylem, clogging the vasculature and causing rapid wilting. Key to preventing colonization are the early defense responses triggered in the host’s root upon infection, which remain mostly unknown. Here, we have taken advantage of a high-throughput in vitro infection system to screen natural variability associated with the root growth inhibition phenotype caused by R. solanacearum in Arabidopsis during the first hours of infection. To analyze the genetic determinants of this trait, we have performed a genome-wide association study, identifying allelic variation at several loci related to cytokinin metabolism, including genes responsible for biosynthesis and degradation of cytokinin. Further, our data clearly demonstrate that cytokinin signaling is induced early during the infection process and cytokinin contributes to immunity against R. solanacearum. This study highlights a new role for cytokinin in root immunity, paving the way for future research that will help in understanding the mechanisms underpinning root defenses. Using genome-wide association analysis, we identify cytokinin as an important component in early root defense responses against the bacterial wilt pathogen Ralstonia solanacearum.
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This study highlights a new role for cytokinin in root immunity, paving the way for future research that will help in understanding the mechanisms underpinning root defenses. Using genome-wide association analysis, we identify cytokinin as an important component in early root defense responses against the bacterial wilt pathogen Ralstonia solanacearum.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/eraa610</identifier><identifier>PMID: 33475698</identifier><language>eng</language><publisher>UK: Oxford University Press</publisher><subject>Research Papers</subject><ispartof>Journal of experimental botany, 2021-03, Vol.72 (7), p.2727-2740</ispartof><rights>The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. 2021</rights><rights>The Author(s) 2021. 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title A genome-wide association study reveals cytokinin as a major component in the root defense responses against Ralstonia solanacearum
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